E ec o Mill Type and Mechanical Kneading Condi ions on Fe men a ion
Kine ics o Te dough Du ing Inje a making and Phy a e o Mine al Mola
Ra io o Inje a
Yoseph Legesse Asse a1,*, Shimelis Admassu Emi e1, Wo kineh Abebe2, Felicidad Ronda3
1Addis Ababa Uni e si y, Addis Ababa Ins i u e o Technology, School o Chemical and Bioenginee ing,
Food Enginee ing G adua e P og am, Addis Ababa, E hiopia
2E hiopian Ins i u e o Ag icul u al Resea ch, Addis Ababa, E hiopia
3College o Ag icul u al and Fo es y Enginee ing, Uni e si y o Valladolid, A . Mad id, Palencia, Spain
Abs ac
Inje a is E hiopian adi ional e men ed la b ead which made mos ly om e lou . Te g ain is
milled and he lou kneaded o ha e ba e -like dough which will e men be o e inje a baking.
The in luence o mill ype: hamme mill (HM), disc mill (DM) and blade mill (BM) and
mechanical kneading speed- ime combina ions (K1, K5, and K9), on e men a ion kine ics was
in es iga ed. Phy a e o mine al mola a io (Fe, Zn and Ca) o e inje a was also in es iga ed as
a ec ed by mill ype and di e en kneading condi ions. In bo h milling and kneading le els,
mal ose was he highes suga concen a ion ini ially, which ollowed by glucose and uc ose. As
e men a ion con inued, a simila end in mal ose b eak down was seen among HM, DM, and BM.
Howe e , di e en pa e ns o glucose and uc ose b eak down we e seen on HM han DM and
BM. Simila ly, HM had a di e en pa e n in inc emen o lac ic acid concen a ion han DM and
BM. Simila end in mal ose concen a ion was seen be ween K1, K5, and K9. Again glucose
b eakdown and he inc emen o lac ic acid in K9 we e di e en han ha o K1 and K5.
Phy a e/mine al mola a io o BM was signi ican ly di e en (p<0.05) om HM and DM. The e
was also a signi ican di e ence (p<0.05) in phy a e/mine al mola a io be ween K1, K5, and K9.
Dec eased phy a e/mine al mola a io was seen wi h inc easing o kneading speed ( pm) o a
longe pe iod o ime. The e ec o mill ype and kneading speed and ime combina ions on
e men a ion kine ics and phy a e/mine al mola a io we e signi ican .
Keywo ds: Te , milling, kneading, e men a ion kine ics, phy a e/mine al mola a io.
1. In oduc ion
Te [E ag os is e ] is an E hiopian indigenous opical ce eal c op and i has been cul i a ed o
many yea s in E hiopian highlands (Demissie, 2001; Viswana h, 2012). P oduc s o e g ain a e
nu i ionally well packed because hey a e always consumed as whole g ain wi h a high con en o
ca bohyd a e and ibe (USDA, 2007), wi h mo e i on, zinc, and calcium han o he ce eal g ains,
including so ghum, whea and ba ley (Abebe e al., 2007). Due o he absence o glu en and glu en-
like p o eins, e has ecen ly been ecei ing global a en ion, pa icula ly as a “heal hy ood”,
making i igh o celiac disease pa ien s (Spaenij-Dekking e al., 2005), and also because o o he
die a y bene i s such as slow- elease o ca bohyd a e cons i uen s use ul o diabe ic pa ien s
(Abebe and Ronda, 2014). Te is he main s aple in he coun y mos ly used o make inje a,
adi ional e men ed la b ead (Bul osa and Taylo , 2004).
Inje a is p epa ed om ba e -like dough, which is p e- e men ed o 2-3 days. Fe men a ion is
mos o he ime ini ia ed spon aneously by addi ion o wa e o e lou , allowing he na u ally
exis ing mic oo ganisms o g ow (Gashe 1985). P ima y o i s s age e men a ion can also be
s a ed by he addi ion o he s a e , e sho, which is a small amoun o ba e kep om he p e ious
dough (Pa ke e al., 1989). Du ing e men a ion s ages, he main e men ing mic oo ganisms a e
lac ic acid bac e ia (Lac obacillus species) (Gashe 1985) and yeas (Saccha omyces species)
(Gi awesen and Bis a 1982). These mic oo ganisms esul is he all o pH, gas p oduc ion and
dough ising and a e esponsible o desi ed inal p oduc la o and acidi y (Ume a and Faulks
1988).
Se e al s udies ha e epo ed he bene icial in luence o e men a ion in imp o ing bo h nu i ional
and sani a y quali ies o oods (Nou , 2009; S anbe g & Lo i, 1997). Acco ding o Nou &
Mo a jemi (1997), P oduc ion o o ganic acids wi h low molecula weigh , such as ace ic and lac ic
acid, educes pH and may hus limi con amina ion by oodbo ne pa hogens. G eine &Konie zny
(2006) also epo ed ha e men a ion can be a eason o he ac i a ion o se e al endogenous
enzymes including phy ases and may hus esul in p oduc s wi h dec eased an i-nu i ional ac o s.
Hammes e al., (2005), s a ed ha he le el o which enzymes like phy ases a e ac i a ed depends
on he e men a ion kine ics, which in u n, depends on he condi ion o aw ma e ials used.
Depending on he mechanical o ces and empe a u e du ing he g inding p ocess, di e en milling
o g inding me hods ha e been seen o p oduce lou s wi h di e en pa icle size and damage
s a ch le el (Kadan e al., 2008). A epo by Abebe e al (2015), a e also s a ed ha lou pa icle
size and deg ee o s a ch damages a e in luenced by mill ypes which a e used o g ind ce eal g ain.
On he o he hand, Li e al., (2014) s a ed lou pa icle size and damaged s a ch ex en a ec
enzyma ic hyd olysis o lou . De la He a e al., (2014) s a ed he pa icle size o lou also a ec
he amoun o ca bon dioxide which e ained du ing e men a ion. Howe e , in o ma ion is lacking
on he e ec s o mill ype on e men a ion kine ics o e dough du ing inje a making p ocess and
i s e ec on mine al o phy a e mola a io since e possessed bo h signi ican ly.
Dough p ocessing is an essen ial ac o which a ec s he quali y o b ead o inje a. Kneading is
one o he mos impo an mechanical s eps in indus ial dough p ocessing (Esselink e al., 2003).
Di e en s udies we e made on he e ec o dough mechanical kneading, on he mo-mechanical
p ope ies o dough’s (Angioloniand Rosa, 2005), dough heological p ope ies (Angioloniand
Rosa, 2007) and b ead quali y (Kim & De Rui e , 1968). All s udies showed ha in luences o
dough kneading condi ions a e signi ican . Howe e , he e ec o mechanical kneading on
e men a ion kine ics and mine al o phy a e mola a io du ing e dough e men a ion is s ill
lacking. The e o e, he aim o his s udy was o in es iga e he in luence o mill ype and
mechanical kneading condi ions on e men a ion kine ics o e dough du ing inje a making
p ocess; and mine al o phy a e mola a io o e inje a.
2. Ma e ials and me hods
2.1 Ma e ials
Based on popula i y among E hiopian e a me s and use s, Qouncho e a ie y (DZ-C -387) was
selec ed and ob ained om Deb eZei Ag icul u al Resea ch Cen e o he E hiopian Ins i u e o
Ag icul u al Resea ch (EIAR). Te sample was he me ically s o ed in cool and d y place using
polye hylene bag. Be o e milling, e g ain was cleaned by si ing.
2.2 Milling p ocess
Hamme mill (HM) (Pe en 120, Finland) i ed wi h 0.8 mm sie e, s one-disk mill (DM) (co age
e g ain-milling, Denma k) and blade mill (BM) (Nu i Bulle NB-101B, China) we e used o ge
he whole lou o e sample. The sample was milled o abou 7 min in case o BM.
2.3 Dough p epa a ion, e men a ion and inje a making
Te dough was p epa ed acco ding o Pa ke e al. (1989) and Zegeye (1997) wi h li le
modi ica ion. Amoun o s a e (E sho) equal o 60ml was ini ially added o each kg o lou . The
e lou ( om s one-disc mill) was mixed 2:3 (w/w) wi h po able wa e and kneaded by ki chen
aid (Moulinex Mas e chi 720, F ance). Dough kneading imes (1, 3 and 7 min) and kneading
speed (Speed 1, 6 and 12) we e selec ed based on inje a expo e s p ac ice and kneading machine
capaci y. Nine di e en e dough’s we e p epa ed using kneading machine by a ying ime and
speed o kneading machine. Based on he senso y esul s o inje a, Kneading # 1 (kneading by
speed 1 o 1 min and gi es mode a e o e all inje a accep abili y), #5 (kneading by speed 6 o 3
min and gi es he highe inje a accep abili y) and #9 (kneading by speed 12 o 7 min and gi es
he lowe inje a accep abili y) we e selec ed o u he s udies.
To s udy he milling e ec s, lou om HM, DM, and BM was mixed simila ly wi h wa e and
kneaded by hand in a bowl un il ob aining a homogenous mix u e in he adi ional way. The dough
was allowed o e men o 60 hou s a oom empe a u e (30 ± 5 ºC).
A e he p ima y e men a ion, 10% o he dough was mixed 1:3 ( / ) wi h boiling wa e , and
hea ed o 15 min wi h con inuous s i ing. The ho cooked dough (absi ) was hen mixed back
in o he e men ing dough, and su icien po able wa e was added o make a ba e . The ba e was
le co e ed o 2 hou s o seconda y e men a ion. Addi ional wa e was added o hin and o m
he igh consis ency o he ba e . Finally, hal a li e o ba e was pou ed on o he ho clay g iddle
in a ci cula o m. A e 2-3 min o cooking ( adi ional elec ic inje a baking equipmen ) inje a
was emo ed and placed in a baske .
2.4 Flou cha ac e iza ion
2.4.1 De e mina ion o phy a es
The colo ime ic me hod as desc ibed by Hang and Lan zseh (1983) was used o de e mine he
phy ic acid con en o he samples.
2.4.2 Mine al de e mina ion
I on, zinc and calcium we e de e mined using me hods 999.11, 968.08 and 985.35 o AOAC
(La ime , 2016).
2.5 Fe men a ion kine ics
2.5.1. Change in pH

As used by Baye e al., (2013), du ing e men a ion he pH o he slu y was eco ded using a pH
me e (Oak on-Eu ech Ins umen s PH6, F ance). The a e o change in pH (_dpH/d ) was
calcula ed o each sample as ollows: _(dpH/d ) = pH( +1) _ pH( )/( +1) _ , whe e ‘‘ ’’ s ands o
ime (hou s). The maximal alue o _(dpH/ d ) o each sample obse a ion was hen a e aged o
gi e he maximal a e o change in pH (_(dpH/d )max).
2.5.2 Ex ac ion p ocedu es
The ex ac ion me hod was ha o Be as (1991) in which a 10 g o sample was homogenized wi h
90 mL dis illed wa e using magne ic ba s i e . Fi e millili e s o 1 mol/L HClO4 solu ion was
added o a 10 mL aliquo o he homogena e. The mix u e was cen i uged o 15 min a 4000 g a
15 1C, he supe na an was neu alized (pH 7.070.1) wi h 2 mol/L KOH and he olume was
adjus ed o 25 mL wi h dis illed wa e . A e 30 min p ecipi a ion on he ice, he solu ion was
il e ed on 0.45 mm cellulose il e (Millipo e).
2.5.3 De e mina ion o suga s, o ganic acids, and e hanol
HPLC analyses we e pe o med wi h an Agilen 1200 model equipped wi h a 20 mL au oma ic
injec ion loop. De ec ion was pe o med wi h a e ac i e index de ec o (RID 156 Beckman)
connec ed o a Shima zu STANG-ST3A in eg a o . Ch oma og aphic sepa a ion was pe o med
using an ICSep ICE-COREGEL 87H3 column (T ansgenomic)(300 mm 7.8 mm, In e ac ion
Ch oma og aphy, F ance) unde he ollowing condi ions: mobile phase 0.001 N H2SO4, low a e
0.6 mL/min, column empe a u e 35 ˚C.
2.6 S a is ical analysis
Analysis o a iance was pe o med on he da a o es ablish signi ican (p <0.05) di e ences
be ween he samples. The sco es we e hen subjec ed o analysis o a iance using SPSS s a is ical
so wa e (Ve sion 16) and means o duplica e esul s we e compa ed by Tukey’s Hones ly
Signi ican Di e ence Tes .
3 Resul s and discussion
3.1 Fe men a ion kine ics
3.1.1 Changes in pH
The kine ics o dec ease in pH showed he same pa e n in all he h ee ypes o dough’s o bo h
milling and kneading a iables (Fig. 1 & 2). Howe e he mean pH a he s a o e men a ion o
BM, was highe (6.41) han ha o DM (6.32) and HM (6.23). On he o he hand, he ini ial dough
pH was no a ec ed by kneading esul ing in he pH o (6.34) o K1, K5 and K9. Du ing he i s
10h o e men a ion, pH dec eased om 6.23 o 4.22 o HM, and om, 6.32 o 4.13 and 6.41 o
4.36 o DM and BM dough espec i ely. Dec ease in pH was also obse ed due o change in
kneading condi ions i.e.; o K1 om 6.34 o 4.16, om 6.34 o 4.1 and om 6.34 o 4.23 o K5
and K9, espec i ely. Acco ding o Nou e al., (1989) he common cha ac e is ic among he
di e en dough’s is a apid d op in pH his may be due o back-slopping e ec s. The maximum
a e o pH dec ease was simila in bo h milling and kneading le els. Howe e , he e men a ion
ime o see highe pH a e o K5 was 2h, which was di e en han ha o K1 and K9 (10h) (Table
1). This di e ence migh be due o he di e ence in kneading speed/ ime combina ion which may
a ec he e men a ion kine ics. In all doughs, he a e a which pH was dec eased when he
e men a ion ex ended om 10 o 24h. In bo h milling and kneading, he pH was dec eased a
slowe a e when e men a ions ex ended om 24 o 48h. Simila esul was epo ed by Baye e
al., (2013). The inal pH o HM, DM, and BM we e 3.47, 3.22 and3.32, espec i ely. Final pH o
3.53, 3.51 and 3.80 was no ed o K1, K2 and K3, espec i ely. Le eb e e al., (2002) s a ed ha ,
when he u iliza ion o e men able ca bohyd a es and he LAB popula ion inc ease, he pH alue
o he inocula ed sou dough dec eased. Le eb e e al. (2002) epo ed he ime necessa y o ob ain
he minimum pH alue was abou 13 h hough he o al i a able acidi y con inued o inc ease un il
19 h and he pH dec ease was co ela ed wi h he p oduc ion o o ganic acids. Acco ding o
Kingamkono e al (1994) and Nou e al, (1989), he pH apidly eached alues below 4.5,
p omo ing be e hygienic condi ions.
Table 1 Kine ic pa ame e s o pH change du ing inje a sou dough e men a ion
HM, DM and BM s and o dough om he hamme , disc, and blade milled lou ; K1, K5, and K9 s and o dough
wi h h ee- ime/ pm kneading combina ions. Da a a e exp essed as mean ± s anda d de ia ions
Figu e 1 Changes in pH du ing inje a sou dough e men a ion. HM, DM and BM s and o dough om hamme , disc
and blade mill. E o ba s ep esen he s anda d de ia ion o means.
0,00
1,00
2,00
3,00
4,00
5,00
6,00
7,00
01210 24 48
pH
Fe men a ion ime (h)
Sample dough
Ini ial ( lou ) pH
(_dpH/d )
max
Time (h) o each
(_dpH/d )max
pH a 48h
Milling
HM
6.23±0.03
0.2±0.00
10
3.47±0.01
DM
6.32±0.00
0.22±0.01
10
3.22±0.00
BM
6.41±0.01
0.21±0.01
10
3.32±0.00
Kneading
K1
6.34±0.01
0.22±0.01
10
3.53±0.00
K5
6.34±0.01
0.24±0.01
2
3.51±0.00
K9
6.34±0.01
0.21±0.01
10
3.80±0.01
Figu e 2 Changes in pH du ing inje a sou dough e men a ion. K1, K5, and K9 s and o dough wi h h ee- ime/ pm
kneading combina ions.. E o ba s ep esen he s anda d de ia ion o means.
3.1.2 Kine ics o subs a e consump ion and p oduc o ma ion
A e e milled using h ee di e en mills, he ini ial suga con en o dough be o e adding s a e
cul u e (E sho) was in es iga ed (Fig 3). In all dough’s ob ained om (HM, DM, BM), he
dominan suga was mal ose which was ollowed by glucose and uc ose. This ag ees wi h a epo
by Baye e al (2013) o ba ley-whea and whea - ed so ghum dough in inje a making. The
concen a ion o mal ose a he s a o e men a ion was 535.23 ppm o HM, 615.68 ppm o DM
and 501.36 ppm o BM. A e which, i s a s dec easing o he inal alues (ze o) in all dough’s
(HM, DM, BM). This ag ees wi h Kulp & Lo enz (2003) epo which s a ed ha mal ose b eaks
down in o glucose molecules du ing sou dough e men a ion. Fo he dough om HM, dec ease
in glucose (22.64 ppm) and uc ose (56.1 ppm) concen a ion was obse ed du ing 2h o
e men a ion. Kulp & Lo enz (2003) explain glucose and uc ose a e he i s suga s o be used
du ing e men a ion. These suga s showed inc emen a 10h o e men a ion, a e which, i s a s
dec easing o each he inal alues o glucose (18.23 ppm) and uc ose (0 ppm). Kulp & Lo enz
(2003) s a ed ha , suc ose is apidly b oken down o glucose and uc ose by yeas enzymes
al eady p esen ou side he cell memb ane; his may jus i y an inc emen shown o glucose and
0,00
1,00
2,00
3,00
4,00
5,00
6,00
7,00
01210 24 48
pH
Fe men a ion ime (h)
HMI and DMI we e no a ied (Table 2). Howe e , BMI had a highe mola a io (Phy a e/i on,
phy a e/zinc, and phy a e/calcium) han ha o HMI and DMI. Al hough he samples had simila
amoun o mine als, he di e ence in phy a e con en which was as esul o using di e en mill
ype jus i ied he eason o ha ing a di e en mola a io. A simila end was seen again be ween
K1, K5, and K9 wi h a signi ican di e ence in phy a e con en which caused by mechanical
kneading lead o ha e di e en mola a io be ween samples. Phy a e/i on mola a io o samples
a ied signi ican ly in he o de o K9 (0.63) < K5 (0.81) < K1 (1.00). The esul showed ha i has
less isk o bioa ailabili y. Hallbe g e al., (1989) also s a ed, phy a e/i on mola a io >1 is
ega ded as indica i e o poo i on bioa ailabili y. Simila ly phy a e/zinc and phy a e/calcium
mola a io o samples we e signi ican ly a ied in he o de o K9 (5.98) < K5 (7.65) < K1 (9.49)
and K9 (1.13) < K5 (1.45) < K1 (1.78), espec i ely. The bioa ailabili y o calcium in HMI, DMI,
and BMI and K1, K5 and K9 we e less. Acco ding o Mo is and Ellis (1985), phy a e/calcium
mola a io >0.24 will impai calcium bioa ailabili y. On he o he hand, Tu nlund e al, (1984)
epo ed ha , zinc abso p ion is g ea ly educed and esul s in a nega i e zinc balance when he
phy a e/ zinc mola a io is 15.
4. Conclusions
The e ec o mill ype du ing inje a making p ocess a ied e men a ion kine ics and dough
composi ion signi ican ly. Fe men a ion pa e n and dough composi ions a e also in luenced by
using di e en mechanical kneading ime and speed combina ions. Bo h e milling and
mechanical kneading me hods play impo an ole in he deg ada ion o phy a e and inc ease
mine al bioa ailabili y. BMI which was made om e lou wi h la ge pa icle size had low
mine als (Fe, Zn and Ca) bioa ailabili y han DMI and HMI. Inc easing o mechanical kneading
speed o a longe pe iod o ime, can dec ease he phy a e con en o inje a and lead o ha e be e

mine al bioa ailabili y. Howe e , al hough phy a e/calcium mola a io dec eased wi h inc ease in
kneading ime and speed (K9), he calcium bioa ailabili y s ill needs o be imp o ed.
ACKNOWLEDGMENTS
The au ho s would like o hank Deb eZei Ag icul u al Resea ch Cen e o he E hiopian Ins i u e
o Ag icul u al Resea ch (EIAR) o p o iding e a ie y and Addis Ababa Uni e si y o
suppo ing wi h acili ies in his esea ch wo k. The au ho s also hank he inancial suppo o he
Minis e io de Economía y Compe i i idad and he Eu opean Regional De elopmen Fund
(AGL2015-63849-C2-2-R) and he Conseje ía de Educacion (Jun a de Cas illa y Leon)/FEDER
(P ojec VA072P17). Ma ina Villanue a hanks he Jun a de Cas illa y Leon o he doc o a e
g an .
Re e ences
Abebe, W., & Ronda, F. (2014).Rheological and ex u al p ope ies o e [E ag os is e (Zucc.)
T o e ] g ain lou gels. Jou nal o Ce eal Science, 60(1), 122-130.
Abebe, W., Colla , C., & Ronda, F. (2015).Impac o a ie y ype and pa icle size dis ibu ion on
s a ch enzyma ic hyd olysis and unc ional p ope ies o e lou s. Ca bohyd a e
polyme s, 115, 260-268.
Abebe, Y., Bogale, A., Hambidge, K. M., S oecke , B. J., Bailey, K., & Gibson, R. S. (2007).
Phy a e, zinc, i on and calcium con en o selec ed aw and p epa ed oods consumed in
u al Sidama, Sou he n E hiopia, and implica ions o bioa ailabili y. Jou nal o Food
Composi ion and Analysis, 20(3), 161-168.
Angioloni, A., & Dalla Rosa, M. (2005). Dough he mo-mechanical p ope ies: in luence o
sodium chlo ide, mixing ime and equipmen . Jou nal o Ce eal Science, 41(3), 327-331.
Angioloni, A., & Dalla Rosa, M. (2007). E ec s o cys eine and mixing condi ions on whi e/whole
dough heological p ope ies. Jou nal o ood enginee ing, 80(1), 18-23.
Axelsson, L., & Ah né, S. (2000). Lac ic acid bac e ia. In Applied mic obial sys ema ics (pp. 367-
388). Sp inge , Do d ech .
Ba b o, N., B i ma ie, S., & Cede blad, Å. K. E. (1985). Reduc ion o he phy a e con en o b an
by lea ening in b ead and i s e ec on zinc abso p ion in man. B i ish Jou nal o
Nu i ion, 53(1), 47-53.
Baye, K. (2014). Te : nu ien composi ion and heal h bene i s (Vol. 67). In l Food Policy Res
Ins .
Baye, K., Mouque -Ri ie , C., Ica d-Ve niè e, C., Roche e, I., & Guyo , J. P. (2013). In luence o
lou blend composi ion on e men a ion kine ics and phy a e hyd olysis o sou dough
used o make inje a. Food chemis y, 138(1), 430-436.
Be as, E. (1991). Mise au poin de le ains bac é iens pou la pani ica ion (Doc o al disse a ion,
Cle mon -Fe and 2).
B une, M., Rossande -Hul én, L., Hallbe g, L., Glee up, A., & Sandbe g, A. S. (1992). I on
abso p ion om b ead in humans: inhibi ing e ec s o ce eal ibe , phy a e and inosi ol
phospha es wi h di e en numbe s o phospha e g oups. The Jou nal o nu i ion, 122(3),
442-449.
Bul osa, G., & Taylo , J. (2004).Pas e and gel p ope ies and in i o diges ibili y o e [E ag os is
e (Zucc.)T o e ] s a ch. S a ch-S ä ke, 56(1), 20-28.
D'Amo e, T., Russell, I., & S ewa , G. G. (1989). Suga u iliza ion by yeas du ing
e men a ion. Jou nal o indus ial mic obiology, 4(4), 315-323.
De la He a, E., Rosell, C. M., & Gomez, M. (2014). E ec o wa e con en and lou pa icle size
on glu en- ee b ead quali y and diges ibili y. Food chemis y, 151, 526-531.
Demissie, A. (2001). Te gene ic esou ces in E hiopia. Na owing The Ri , 27-31.
Esselink, E., an Aals , H., Maliepaa d, M., Hende son, T. M., Hoeks a, N. L., & an Duynho en,
J. (2003). Impac o indus ial dough p ocessing on s uc u e: a heology, nuclea magne ic
esonance, and elec on mic oscopy s udy. Ce eal Chemis y, 80(4), 419-423.
Gashe, B. A. (1985). In ol emen o Lac ic Acid Bac e ia in he Fe men a ion o TEF (E ag os is
e ), an E hiopian Fe men ed Food.Jou nal o ood science, 50(3), 800-801.
Gi awesen, C., & Bes a , A. (1982). Yeas lo a o e men ing e (E ag os is Te ) dough. SINET:
E hiop. J. Sci., 5(1)), 21-25.
Gobbe i, M., Co se i, A., & Rossi, J. (1994). The sou dough mic o lo a. E olu ion o
ca bohyd a es du ing he sou dough e men a ion. Mic obiologie, Alimen s, Nu i ion, 12,
9-15.
Gobbe i, M., Simone i, M. S., Co se i, A., San inelli, F., Rossi, J., & Damiani, P. (1995). Vola ile
compound and o ganic acid p oduc ions by mixed whea sou dough s a e s: in luence o
e men a ion pa ame e s and dynamics du ing baking. Food Mic obiology, 12, 497-507.
G eine , R., & Konie zny, U. (2006). Phy ase o ood applica ion. Food Technology &
Bio echnology, 44(2).
Hallbe g, L., B une, M., & Rossande , L. (1989). I on abso p ion in man: asco bic acid and dose-
dependen inhibi ion by phy a e. The Ame ican jou nal o clinical nu i ion, 49(1), 140-
144.
Hammes, W. P., B and , M. J., F ancis, K. L., Rosenheim, J., Sei e , M. F., & Vogelmann, S. A.
(2005). Mic obial ecology o ce eal e men a ions. T ends in Food Science &
Technology, 16(1-3), 4-11.
Hang, W., & Lan zsch, H. J. (1983). Compa a i e me hods o he apid de e mina ion o oxala e
and phy a e in ce eal p oduc s. J. Sci. Food Ag ic, 34, 1423-1426.
Kadan, R. S., B yan , R. J., & Mille , J. A. (2008). E ec s o milling on unc ional p ope ies o
ice lou . Jou nal o ood science, 73(4).
Kim, J. C., & De Rui e , D. (1968). B ead om non-whea lou s. Food Technol, 22(7), 867-878.
Kingamkono, R., Sjög en, E., S anbe g, U., & Kaijse , B. (1994). pH and acidi y in lac ic-
e men ing ce eal g uels: e ec s on iabili y o en e opa hogenic mic oo ganisms. Wo ld
Jou nal o Mic obiology and Bio echnology, 10(6), 664-669.
Kulp, K., & Lo enz, K. (Eds.). (2003). Handbook o dough e men a ions (Vol. 127). C c P ess.
La ime , G. W. (2016). O icial me hods o analysis o AOAC In e na ional. Rock ille, MD:
AOAC In e na ional, ISBN: 978-0-935584-87-5.
Le eb e, D., Gab iel, V., Vayssie , Y., & Fon agne-Fauche , C. (2002). Simul aneous HPLC
de e mina ion o suga s, o ganic acids and e hanol in sou dough p ocess. LWT-Food
Science and Technology, 35(5), 407-414.
Li, E., Dhi al, S., & Hasjim, J. (2014). E ec s o g ain milling on s a ch s uc u es and lou /s a ch
p ope ies. S a ch‐S ä ke, 66(1-2), 15-27.
López, A. C. B., Pe ei a, A. J. G., & Junquei a, R. G. (2004). Flou mix u e o ice lou , co n and
cassa a s a ch in he p oduc ion o glu en- ee whi e b ead. B azilian A chi es o Biology
and Technology, 47(1), 63-70.
Ma, G., Jin, Y., Piao, J., Kok, F., Guusje, B., & Jacobsen, E. (2005). Phy a e, calcium, i on, and
zinc con en s and hei mola a ios in oods commonly consumed in China. Jou nal o
Ag icul u al and Food Chemis y, 53(26), 10285-10290.
Majzoobi, M., Pashangeh, S., Fa ahnaky, A., Eskanda i, M. H., & Jamalian, J. (2014). E ec o
pa icle size educ ion, hyd o he mal and e men a ion ea men s on phy ic acid con en
and some physicochemical p ope ies o whea b an. Jou nal o ood science and
echnology, 51(10), 2755-2761.
Ma hewson, P. R. (2000). Enzyma ic ac i i y du ing b ead baking. Ce eal Foods Wo ld.
MORRIS, E. R., & ELLIS, R. (1985). Bioa ailabili y o die a y calcium: e ec o phy a e on adul
men consuming non ege a ian die s.
Nou , M. J. R., & Mo a jemi, Y. (1997). Assessmen o e men a ion as a household echnology
o imp o ing ood sa e y: a join FAO/WHO wo kshop. Food Con ol, 8(5-6), 221-226.
Nou , M. R. (2009). Rich nu i ion om he poo es –Ce eal e men a ions in A ica and Asia. Food
Mic obiology, 26(7), 685-692.
Nou , M. R., Rombou s, F. M., & Ha elaa , A. (1989). E ec o accele a ed na u al lac ic
e men a ion o in an ood ing edien s on some pa hogenic mic oo ganisms. In e na ional
Jou nal o Food Mic obiology, 8(4), 351-361.
O lowski, J. H., & Ba o d, J. P. (1987). The mechanism o up ake o mul iple suga s by
Saccha omyces ce e isiae in ba ch cul u e unde ully ae obic condi ions. Applied
mic obiology and bio echnology, 25(5), 459-463.
Pa ke , M. L., Ume a, M., & Faulks, R. M. (1989). The con ibu ion o lou componen s o he
s uc u e o inje a, an E hiopian e men ed b ead made om e (E ag os is e ). Jou nal
o Ce eal Science, 10(2), 93-104.

Rasane, P., Jha, A., Sabikhi, L., Kuma , A., & Unnik ishnan, V. S. (2015). Nu i ional ad an ages
o oa s and oppo uni ies o i s p ocessing as alue added oods-a e iew. Jou nal o ood
science and echnology, 52(2), 662-675.
Röcken, W., Rick, M., & Reinkemeie , M. (1992). Con olled p oduc ion o ace ic acid in whea
sou doughsKon ollie e Essigsäu ebildung in Weizensaue eigen. Zei sch i ü
Lebensmi el-Un e suchung und Fo schung, 195(3), 259-263.
Roge s D, Langemeie J. (1995). Suga s in doughs and esul an b eads and biscui s. Technical
Bulle in, Ame ican Ins i u e o Baking Resea ch Depa men 17(5):1-5.
Se ano, R., & Dela uen e, G. (1974). Regula o y p ope ies o he cons i u i e hexose anspo in
Saccha omyces ce e isiae. Molecula and cellula biochemis y, 5(3), 161-171.
Spaenij-Dekking, L., Kooy-Winkelaa , Y., & Koning, F. (2005).The E hiopian ce eal e in celiac
disease. New England Jou nal o Medicine, 353(16), 1748-1749.
S anbe g, U., & Lo i, W. (1997). Fe men a ion and nu ien a ailabili y. Food Con ol, 8(5-6),
319-327.
Tu nlund, J. R., King, J. C., Keyes, W. R., Gong, B., & Michel, M. C. (1984). A s able iso ope
s udy o zinc abso p ion in young men: e ec s o phy a e and a-cellulose. The Ame ican
jou nal o clinical nu i ion, 40(5), 1071-1077.
Ume a, M., & Faulks, R. M. (1988).The e ec o e men a ion on he ca bohyd a es in e
(E ag os is e ).Food chemis y, 27(3), 181-189.
U ga, K., & Na asimha, H. V. (2017). T adi ional sou dough b ead (Di o Dabbo) making: I.
E ec s on phy ic acid des uc ion. The E hiopian Jou nal o Heal h De elopmen
(EJHD), 12(3).
USDA, (2007).Na ional Nu ien Da abase o S anda d Re e ence.Release 20.USDA.
Viswana h, N. (2012). The Hie a chy o Poo : The Tension Be ween Fa o ing Smallholde
Fa me s o Domes ic Consume s in E hiopian Ag icul u al De elopmen . A ica Policy
Jou nal, 8, 30.
Waley, S. G. (1981). An easy me hod o he de e mina ion o ini ial a es. Biochemical
Jou nal, 193(3), 1009-1012.
Zegeye, A. (1997). Accep abili y o inje a wi h s ewed chicken.Food quali y and p e e ence, 8(4),
293-295.