Water relations composition among Egyptian cotton genotypes under water deficit

ACTA AGRARIA DEBRECENIENSIS 2021-2 DOI: 10.34101/ACTAAGRAR/2/9194 5 Wa e ela ions composi ion among Egyp ian co on geno ypes unde wa e de ici Maamoun A. Abdel-Moneam1 – Waleed A. E. Abido1* – Mohammed H. Ghoneima1 – Ágnes Hadházy2 – László Zsombik2 – Yase M. El-Mansy3 – Mohab W. El-Shazly3 1Ag onomy Depa men , Facul y o Ag icul u e, Mansou a Uni e si y, Egyp 2Resea ch Ins i u e o Nyi egyhaza, Ins i u es o Ag icul u al Resea ch and Educa ional Fa m, Uni e si y o Deb ecen, Hunga y 3Co on Resea ch Ins i u e, Ag icul u al Resea ch Cen e , Egyp *Co espondence: [email protected] SUMMARY Backg ound: wa e sho age is one o he majo ac o e ec s on g ow h cha ac e s and yield o mos c ops. Objec i e: his s udy was conduc ed o ge o know he eac ions o some Egyp ian co on geno ypes o wa e de ici . Me hods: The gene ic ma e ials used in his s udy included hi een co on geno ypes belonging o Gossypium ba badense L., om he Co on Resea ch Ins i u e (CRI), which was de o ed o es ablishing he expe imen al ma e ials o his in es iga ion. Resul s: he a io o GCA/SCA was less han uni y o all s udied indices, indica ing p edominance o non-addi i e gene ac ion (dominance and epis asis), which is an impo an in exploi a ion o he e osis h ough hyb id b eeding. Resul s: The da a showed signi ican educ ion in wa e ela ionship cha ac e s o all pa en al geno ypes unde s ess condi ions. The Egyp ian a ie y Giza 68 ga e high alues o mos wa e ela ionship cha ac e s. Da a e ealed ha he g ea e he alue o ole ance index is, he la ge he yield educ ion is unde wa e de ici condi ions and he highe he s ess sensi i i y is becoming. The pa en al geno ypes Giza 96 showed he highes educ ion in yield unde wa e de ici condi ions. A he same ime, he c oss combina ion Minu y x Aus aly showed highe alues o yield educ ion ollowed by he combina ions Giza 67 x Aus aly. O he male pa en s, he Russian geno ype 10229 eco ded he bes GCA alues o mos wa e ela ionship cha ac e s. A he same ime, he emale pa en s, he old Egyp ian geno ype Giza 67 eco ded he bes alues and exhibi ed good gene al combined o mos wa e ela ionship cha ac e s. The c oss combina ions Giza 86 x Pima S6, Giza 77 x Pima S6, Giza 94 x Dand a and Giza 96 x Aus aly showed signi ican desi able SCA e ec o mos cha ac e s. Conclusion: ela i e wa e con en %, osmo ic p essu e, chlo ophyll and ca o enoids con en indica es be e a ailabili y o wa e in he cell, which inc eases he pho osyn he ic a e. Also, he highe le el o p oline accumula ion in he lea es which was eco ded unde de ici wa e sugges s ha he p oduc ion o p oline is p obably a common esponse o plan unde wa e de ici condi ions. Keywo ds: co on, wa e ela ions, co ela ion coe icien s, gene al combining abili y, speci ic combining abili y INTRODUCTION Wa e sca ci y (sho age) is one o he majo limi ing ac o s o c op de elopmen and yield. The an icipa ed demand in addi ional wa e supplies o ag icul u al p oduc ion will lead o inc ease wa e sca ci y in nea u u e. Thus, i iga ed c op p oduc ion such as co on sys em needs a be e managemen o inc ease wa e use e iciency. B eeding o imp o e d ough ole an geno ypes equi es o iden i ica ion o physiological mechanisms and mo phological cha ac e s con e ing d ough ole ance. In his ega d, (Iqbal e al., 2011) de ec ed signi ican di e ences among 80 co on geno ypes o indices o d ough ole ance. Howe e , (Kashi e al., 2012) no iced signi ican a ia ion o wa e egimes wi h espec o some chemical and p oduc i i y cha ac e s. Also, (Amjid e al., 2016) s a ed ha he co on geno ypes di e ed signi ican ly o ela i e wa e con en , excised wa e loss and cell memb ane s abili y. I is in e es ing o no e ha , he co on pa en s which showed high alues o RWC% in each condi ion exhibi high alues in o al wa e con en , ee wa e and bound wa e %, also showed educed alues in LWD alues as compa ed wi h he o he pa en s. This due o oxida i e inju y a he cellula le el unde wa e s ess has high lipid pe oxida ion, which dec eased s abili ies o cell memb ane and led o loss mo e wa e om cell (Sanchez e al., 2002; Abdel-Kade e al., 2015b). The osmo ic p essu e is conside ed as one o he impo an mechanisms o wa e de ici ole ance o plan (Khan e al., 2015), which p omo es he p o ec ion o he plan cell s uc u es including memb ane and chlo oplas s. Plan s adjus o wa e s ess by lowe ing issue osmo ic po en ials by he accumula ion o ino ganic ions and /o o ganic subs ance o pe mi he main enance o cell u go . Chlo ophyll con en s as chlo ophyll A and B plays a i al ole in pho osyn he ic p ocess which ul ima ely inc ease c op g ow h and yield. The ad e se e ec o wa e de ici on chlo ophyll con en has p e iously been showed by (Pi zad e al., 2011; Abdel- Kade e al., 2015a; Kannan e al., 2017). Howe e , (Hamayun e al., 2010) s udied he e ec o d ough s ess on chlo ophyll and ound ha wa e s ess had dec eased chlo ophyll A, B and o al chlo ophyll. Te minal o s ess occu ing du ing he p oduc i e phase, lowe ing and boll, is known o induce boll educ ion and educe on boll size and weigh as well as boll 3 shedding (Zangi, 2010). Besides, he e is a signi ican dec ease in RWC% unde wa e de ici condi ions was due o educed abso p ion o wa e om he soil and inabili ies o con ol wa e loss h ough he s oma a (Rahman e al., 2008; Anan hi and Vijaya agha an, 2012; Kuma e al., 2013; Hu e al., 2013). In addi ion, unde wa e s ess condi ions, he geno ypes showed signi ican dec eased in me abolic ac o s, such as dec eased in chlo ophyll (A, B), ca o enoids con en and enhanced accumula ion o p oline (Din e al., 2011). Indi ec selec ion o s ess en i onmen based on pe o mance o i iga ed ABDEL-MONEAM, M.A. ET AL. ACTA AGRARIA DEBRECENIENSIS 2021-2 DOI: 10.34101/ACTAAGRAR/2/9194 6 condi ions would be e ec i e (Anwa e al., 2011). Thus, he p e- equisi e o success equi es de e mina ion o he ex en o geno ypic a ia ion wi hin geno ypes o hese ai s and hei ela i e con ibu ion o economic yield. The impac o wa e de ici (a ailabili ies a a m ga e) and insu icien a ailabili y o wa e du ing he sowing pe iod seems o be he main eason o lessa ac eage co on c op and educ ion in co on p oduc ion. The e o e, b eeding o d ough ole ance co on is an impo an ask and objec i e. Fo e ec i e b eeding o ole an wa e de ici co on a ie ies good selec ion c i e ia is needed o iden i y he ole an co on geno ypes. MATERIALS AND METHODS The p esen s udy was done in he Ag onomy Depa men , Facul y o Ag icul u e, Mansou a Uni e si y. The in es iga ion was ca ied ou a Sakha Ag ic. Res. S a . Ka EL-Sheikh, Ag ic. Res. Cen e , Egyp , du ing 2014 and 2015 g owing seasons. Gene ic ma e ials and expe imen al p ocedu e: The gene ic ma e ials used in his s udy included hi een co on geno ypes belonging o Gossypium ba badense L., om he Co on Resea ch Ins i u e (CRI), which de o ed o es ablish he expe imen al ma e ials o his in es iga ion. Expe imen al design and labo a o y p ocedu es: The hi een pa en s we e c ossed, in such away Line x Tes e (9x4) ma ing design by using ou pa en s as a Tes e pa en s i.e. Dand a, Pima S6, Aus aly and 10229 and nine co on geno ypes as a Line pa en s i.e. Giza 45, Minu i, Giza 67, Giza 68, Giza 86, Giza 77, Giza 94, Giza 96 and Giza 69, in 2014 c op season o p oduce 36 F1 hyb id seeds, and he o iginal pa en s we e also sel ed. In 2015 season he 36 F1 hyb ids and hei pa en s we e g own in a andomized comple e block design (RCBD) wi h h ee eplica es unde wo i iga ed condi ions. The i s one is he no mal i iga ed 7 i iga ions du ing he g owing season and he second is he s ess condi ion, h ee i iga ions only du ing he g owing season. Each expe imen al plo consis ed o one ow, measu ing i e me e s in leng h and 0.70 m in wid h, wi h plan s spaced 30 cm wi hin ow. Two plan s we e le pe hill a 4 hinning ime. The ob ained da a we e subjec ed o di e en s a is ical and biome ical echniques acco ding o hei ma ing sys em. A sepa a e analysis o a iance o each cha ac e a each en i onmen was done (Pelc e al., 1997) o de ec he signi icance o he obse ed di e ences. Fu he line x es e analysis as p oposed by (Kemp ho ne, 1957) and adop ed by (Singh and Chaudha y, 1977) was de ia ed o pa i ioning he gene ic a iance o he F1 op c osses due o lines, es e s and hei in e ac ions, p o ide in o ma ions abou gene al and speci ic combining abili ies o he pa en s and c osses (Table 1). In addi ion, i also p o ides addi ional in o ma ions abou he a ious ypes o gene e ec s. Table 1. The o m o he analysis o a iance o line x es e analysis S.O.V D M.S E.M.S Replica ion -1 MS Geno ypes g-1 MSg σ2e + σ2g Pa en s p-1 σ2e + σ2p C osses c-1 σ2e + σ2e Pa en s s C osses 1 Lines l-1 M1 σ2e + ((Co .F.s-2Co .H.s) + (Co .H.s)) Tes e s -1 M2 σ2e + ((Co .F.s-2Co .H.s) + l (Co .H.s)) Lines x Tes e s (l-1)( -1) M3 σ2e + ((Co .F.s-2Co .H.s) E o ( -1)(g-1) M4 σ2e Whe e, , g, p, c a e he numbe o eplica ions, geno ypes, pa en s and c osses espec i ely.; L = numbe o lines ( emale pa en s).; = numbe o es e s (male pa en s).; σ2e = plo en i onmen al a iance. Gene ic componen s Acco ding o (Kemp ho ne, 1957; Kea sey and Pooni, 1996) he a iance o gene al and speci ic combining abili ies o each expe imen was compu ed om he co a iance's o ull and hal sib amilies as ollows: Co Hs (lines) = M1−M3 ; Co Hs ( es e s) = M2−M3 l ; Co Hs (a e age) = 1 (2l −l− ){(l−1)(Ml)+( −1)(M ) l+ −2 −Ml } σ2 gca = Co Hs = (1+F) 4 = 1 2σ2A ; σ2 sca = Ml −Me = Co ull sib.; σ2 D = Co ull sib - 2Co Hs.; σ2 sca = (1+F 2)2= σ2 D σ2 D = σ2 sca ACTA AGRARIA DEBRECENIENSIS 2021-2 DOI: 10.34101/ACTAAGRAR/2/9194 7 whe e, Co Hs = is he co a iance hal sibs.; Co Fs = is he co a iance ull sibs.; σ2gca, σ2sca = a e he a iance o gene al and speci ic combining abili y.; σ2A, σ2 D = a e he componen o gene ic a iance due o addi i e and dominance a iances.; F = is he coe icien o inb eeding which was conside ed equal one.; σ2 G = σ2 A + σ2 D Es ima es o he e osis The alues o he e osis we e de e mined as he pe cen age de ia ion om he F1s hyb ids o e he be e pa en s (B.P.) as ollow: H (B.P.) = F1−B.P B.P 𝑋100. The signi icance o he e osis was es ed using he leas signi ican di e ences alue (L.S.D) a 0.5% le el o p obabili ies acco ding o he o mula o (S eel e al., 1997) L.S.D a 0.05 = 0.05 x sd , sd = √2𝑀𝑠𝑒 𝑟 Gene al and speci ic combining abili ies e ec s a. Gene al combining abili ies: Lines g1 = x1.. −x.. l Tes e s gj = xj… l −x… l Whe e, g1= is he gene al combining abili y e ec .; Xi... = is he o al alue o c osses in which he line in ol ed o e eplica ions.; Xj... = is he o al alue o c osses in which he es e in ol ed o e eplica ions.; X... = is he gene al o al o c osses; R, l, = a e he numbe o eplica ions, line and es e s espec i ely. b. Speci ic combining abili y: Sij = xij.. −xi.. −xj.. l −x.. l Whe e, Sij= is he speci ic combining abili y e ec s; Xij = is he o al alue o c osses be ween lines and es e s o e eplica ions. RESULTS AND DISCUSSION Analysis o a iance o wa e ela ions lea es chemical composi ion and d ough ole ance indices a e shown in Tables 2 and 3. The da a e ealed signi ican di e ences among he geno ypes o all s udied cha ac e s, indica ing he p esence o conside able amoun o gene ic a iabili ies among he e alua ed geno ypes. Such a ia ion could be a ibu ed o he a ied gene ic backg ound. Fu he pa i ioning o geno ypes means squa es o hei componen s exhibi ed ha pa en s mean squa es we e signi ican o all s udied cha ac e s unde s ess and no mal condi ions, showing su icien a iabili ies among he pa en s which can gene a e po en ial and p omising hyb ids. The a iance due o males and emales we e also signi ican o mos s udied cha ac e s and majo i y han he a iance due o in e ac ions (lines x es e s), hese esul s indica ed ha he expe imen al ma e ials possessed conside able a iabili ies and he wo ypes o combining abili ies we e in ol ed in he gene ic exp ession o hese cha ac e s. The es e s con ibu ed a majo sha e o he gene ic a iance in espec o mos physiological and chemical cha ac e s unde s ess and non-s ess condi ions. In his ega d, (Iqbal e al., 2011; Kashi e al., 2012; Amjid e al., 2016). Table 2. Analysis o a iance o line x es e and combining abili y o wa e ela ions and lea es chemical composi ion unde no mal and wa e s ess condi ions S.O.V D To al wa e con en (%) F ee wa e (%) Bound wa e (%) Lea wa e de ici (%) Rela i e wa e con en (%) N S N S N S N S N S Replica ion 2 0.14 0.12 0.05 0.01 0.36 0.06 0.01 0.14 17.52** 7.70** Geno ypes 48 67.50** 21.96** 2.34** 0.48** 45.85** 17.45** 3.52** 5.62** 74.86** 14.31** Pa en s 12 4.28** 3.10** 0.49** 0.21** 1.99** 2.38** 2.81** 1.90** 4.78** 2.99** C osses 35 27.15** 14.08** 1.03** 0.52** 18.92** 9.94** 1.76** 1.69** 30.73** 12.16** Pa en s s C osses 1 2238.3 523.6** 70.38** 2.01** 1514.9** 460.99** 73.77** 187.70** 2460.4** 225.50** Lines 8 24.99** 10.95** 1.06** 0.50** 16.75** 7.28** 2.27** 1.26** 26.35** 8.93** Tes e s 3 112.71** 21.87** 1.49** 0.32** 89.58** 17.01** 2.56** 2.80** 79.07** 16.96** Lines x Tes e s 24 17.17** 14.16** 0.96** 0.56** 10.80** 9.94** 1.49** 1.69** 26.14** 12.64** E o 96 1.15 0.85 0.05 0.02 0.88 0.68 0.10 0.11 2.36 1.20 σ2 GCA 0.18 -0.001 0.001 -0.001 0.15 -0.0001 0.01 -0.0001 0.1 -0.01 σ2 SCA 5.29 4.46 0.30 0.18 3.29 3.12 0.47 0.53 7.68 3.76 σ2 GCA/ σ2 SCA 0.03 -0.0002 0.003 -0.006 0.05 0.00 0.02 -0.0002 0.01 -0.003 CV % 1.27 1.33 1.91 1.62 1.28 1.37 3.54 3.31 2.02 1.81 ABDEL-MONEAM, M.A. ET AL. ACTA AGRARIA DEBRECENIENSIS 2021-2 DOI: 10.34101/ACTAAGRAR/2/9194 8 Table 2. con inued S.O.V D Osmo ic p essu e (ba ) Chlo ophyll A (mg g-1 dw ) Chlo ophyll B (mg g-1 dw ) Ca o enoides (mg g-1 dw ) P oline (mg g-1 w ) N S N S N S N S N S Replica ion 2 0.003 0.02 0.01 0.001 0.001 0.0001 0.003 0.001 9.47 188.30 Geno ypes 48 1.47** 1.46** 0.29** 0.10** 0.02** 0.01** 0.04** 0.02** 5014.6** 12172.9** Pa en s 12 0.73** 0.60** 0.10** 0.01* 0.01** 0.01** 0.01** 0.003** 201.58** 108.66 C osses 35 0.30** 0.42** 0.16** 0.12** 0.02** 0.02** 0.03** 0.018** 4725.39** 4713.9** Pa en s s C osses 1 51.35** 48.15** 7.02** 0.36** 0.33** 0.079** 1.05** 0.23** 72891.88** 418009.02** Lines 8 0.38** 0.31** 0.16** 0.12** 0.02** 0.02** 0.03** 0.02** 6050.74** 3523.62** Tes e s 3 0.45** 0.70** 0.13** 0.09** 0.02** 0.003** 0.02** 0.03** 6815.01** 7810.29** Lines x Tes e s 24 0.25** 0.42** 0.17** 0.12** 0.02** 0.02** 0.02** 0.02** 4022.40** 4723.55** E o 96 0.013 0.024 0.007 0.004 0.001 0.0004 0.001 0.001 189.40 229.02 σ2 GCA 0.001 -0.00 -0.0001 0.0001 -0.00 -0.00 0.00 0.00 12.64 -0.17 σ2 SCA 0.08 0.13 0.05 0.04 0.01 0.01 0.01 0.01 1257.21 1474.68 σ2 GCA/ σ2 SCA 0.01 0.00 -0.002 0.003 0.00 0.00 0.00 0.00 0.0101 -0.0001 CV % 2.96 3.13 2.25 2.08 1.77 1.56 2.32 2.22 4.37 2.56 *, ** Signi ican a 0.05 and 0.01 p obabili y le els, espec i ely, N = no mal i iga ion and S = wa e s ess Table 3. Analysis o a iance o he s udied co on geno ypes o d ough ole ance indices unde no mal and wa e s ess condi ions S.O.V D Tol M.P. H.M. S.S.I. G.M.P. S.T.I. Y.I. Y.S.I R.D.I D.I. Replica ion 2 38.76 17.30 10.87 0.05 13.58 0.003 0.003 0.003 0.01 0.01 Geno ypes 48 309.65** 376.38** 391.45** 0.55** 381.21* 0.03** 0.10** 0.03** 0.05** 0.12** Pa en s 12 367.24** 418.59** 482.05** 0.93** 446.41* 0.05** 0.13** 0.05** 0.09** 0.17** C osses 35 293.22** 299.66** 298.22** 0.43** 296.44** 0.02** 0.07** 0.02** 0.04** 0.10** Pa en s s C osses 1 193.62** 2554.96** 2567.36** 0.06 2565.43* 0.003 0.44** 0.003 0.01 0.22** Lines 8 558.07** 168.81** 150.72** 0.81** 155.63** 0.04** 0.04** 0.04** 0.07** 0.11** Tes e s 3 175.06** 490.64** 586.95** 0.49** 537.90** 0.03** 0.17** 0.03** 0.05** 0.20** Lines x Tes e s 24 219.70** 319.40** 311.29** 0.30** 313.20** 0.02** 0.07** 0.02** 0.03** 0.08** E o 96 34.28 44.70 42.84 0.05 43.46 0.003 0.009 0.003 0.01 0.01 σ2 GCA 1.32 -0.36 -0.24 0.002 -0.30 0.0001 0.00 0.0001 0.0002 0.0003 σ2 SCA 60.11 93.31 91.27 0.08 91.69 0.004 0.02 0.004 0.01 0.02 σ2 GCA/ σ2 SCA 0.02 -0.004 -0.003 0.03 -0.003 0.03 0.00 0.03 0.02 0.02 CV % 26.69 8.99 9.05 20.68 8.99 7.05 9.90 7.05 7.04 14.15 *, ** Signi ican a 0.05 and 0.01 p obabili y le els, espec i ely, N = no mal i iga ion and S = wa e s ess D ough indices which p o ide a measu e o d ough based on loss o yield unde d ough condi ions in compa es o no mal condi ions. The analysis o a iance (Table 3) showed highly signi ican o all d ough ole ance indices, which indica ed ha geno ypes we e di e ing o genes con olling such cha ac e s. Signi ican di e ences we e de ec ed among pa en s, hyb ids and pa en 's s hyb ids o mos indices, indica ing he exis ence o a iabili ies among pa en s and ansmi ed o c oss combina ions. Es ima es o a iances due o gene al and speci ic combining abili ies o indices unde s udy a e p esen ed in Table 3. Gene al combining abili y o emale pa en s, es e s, we e highly signi ican o all s udied indices and la ge in magni ude han male pa en s. Howe e , SCA a iances which due o in e ac ions o line x es e s we e also high signi ican o all s udied indices. The a io o GCA/SCA was less han uni y o all s udied indices, indica ing p edominance o non-addi i e gene ac ion (dominance and epis asis), which is an impo an in exploi a ion o he e osis h ough hyb id b eeding. Hence, subs an ial imp o emen in hese indices may be achie ed h ough ecu en selec ion ollowed by selec ion unde s ess condi ions. The SCA a iances we e g ea e han GCA o all s udied cha ac e s unde s ess and non-s ess condi ions, indica ing mo e impo an ole o non- addi i e ype o gene e ec . The p opo ional con ibu ions o lines ( emales) and es e s (males) and hei in e ac ions o he o al a iance o di e en cha ac e s a e p esen ed in Tables 4 and 5. The da a e ealed ha he maximum con ibu ion o he o al a iance o mos s udied cha ac e s in bo h no mal and s ess condi ions we e made by lines x es e s (male x emale in e ac ion). A he same ime, he 7 con ibu ion due o line pa en s we e g ea e han con ibu ion due o es e s in he s ess condi ion. The physiological pa ame e s such as ela i e lea wa e con en (RWC), lea wa e de ici (LWD), osmo ic p essu e (OPCS), chlo ophyll A, chlo ophyll B, ca o enoids and lea p oline accumula ion a e some sensi i e physiological and biochemical indica o used o s udy he esponse o co on plan s unde s ess condi ion. The da a p esen ed ACTA AGRARIA DEBRECENIENSIS 2021-2 DOI: 10.34101/ACTAAGRAR/2/9194 9 in Table 6 show signi ican educ ion in wa e ela ionship cha ac e s o all pa en al geno ypes unde s ess condi ion. The highes RWC% was obse ed by he pa en s Giza 77 ollowed by Giza 86 and Giza 67 unde no mal and s ess condi ions. On he o he side, he Egyp ian ex a-long s aple a ie y Minu y and Giza 96 showed he lowes alues o RWC% unde no mal and s ess condi ions. The Egyp ian a ie y Dand a showed dec eased in RWC% and LWD%. The Egyp ian a ie y Giza 68 (as a common pa en o mos ex a-long a ie ies) ga e high alues o mos wa e ela ionship cha ac e s. The obse ed signi ican dec ease in RWC% unde wa e de ici condi ions was due o educed abso p ion o wa e om he soil and inabili ies o con ol wa e loss h ough he s oma a. Simila esul s we e ob ained by (Anan hi and Vijaya agha an, 2012; Kuma e al., 2012, Hu e al., 2013). I is in e es ing o no e ha , he co on pa en s which showed high alues o RWC% in each condi ion exhibi high alues in o al wa e con en , ee wa e and bound wa e %, also showed educed alues in LWD alues as compa ed wi h he o he pa en s. This due o oxida i e inju y a he cellula le el unde wa e s ess has high lipid pe oxida ion, which dec eased s abili ies o cell memb ane and led o loss mo e wa e om cell (Abdel-Kade e al., 2015b). The highes RWC% was eco ded by he c oss combina ions Giza 77 x Pima S6 ollowed by Giza 86 x Dand a, Giza 86 x 10229 and Giza 99 x Dand a unde well i iga ed and de ici condi ions. These combina ions su passed all c osses in wa e ela ion cha ac e s unde s ess and no mal condi ions. Geno ypic a ia ion o lea wa e ela ion may be a ibu ed o he di e ences in abili ies o abso p ion mo e wa e om he soil and he abili ies o educe wa e loss h ough s oma a. I may be also due o di e ences in abili ies o geno ypes o main ain issue ige and hence physiological ac i i ies (Khan e al., 2015). Da a illus a ed in Table 6 e ealed ha all 13 co on pa en s exhibi ed some deg ee o osmo ic adjus men in esponse o wa e de ici . The pa en s Giza 77, Giza 68 and Giza 67 showed he lowe ing alues o osmo ic p essu e in cell issue unde no mal and de ici condi ions. Howe e , he pa en s Dand a, Giza 69, Giza 96 and Aus aly mani es ed high osmo ic alues o e bo h condi ions. The imp o emen o osmo ic p essu e usually indica es highe wa e e en ion capaci y and a lowe a e o wa e loss wi h highe RWC%. The c oss-combina ion Giza 67 x PimaS6 ollowed by Giza 68 x PimaS6, Giza 86 x 10229, Giza 94 x Dand a and Giza 77 x PimaS6 eco ded he lowes alues o osmo ic p essu e unde no mal and s ess condi ions. These c oss combina ions showed some so o ole an wa e de ici . On he o he side, he combina ions Giza 45 x PimaS6 ollowed by Giza 86 x Dand a, Giza 69 x Dand a and Giza 69 x Aus aly showed he highes O.P.C.S alues. Simila esul s was ob ained by (Rahman e al., 2008). Chlo ophyll con en s as chlo ophyll A and B plays a i al ole in pho osyn he ic p ocess which ul ima ely inc ease c op g ow h and yield. Da a illus a ed in Table 6 showed ha unde wa e de ici condi ions. Chlo ophyll A and B alues as mg g-1 d y weigh we e educed o he lowes amoun . The pa en al co on geno ypes di e ed signi ican ly o chlo ophyll con en s unde no mal and s ess condi ions. The highes alues o chlo ophyll A we e eco ded by Giza 77 ollowed by Giza 68 and Giza 86 unde no mal and s ess condi ions. On he o he side, he lowes alues we e eco ded o Giza 96 ollowed by Giza 69 and Minu y unde no mal and s ess condi ions. A he same ime, he alues o chlo ophyll B we e signi ican ly di e en among co on geno ypes. The pa en s which, eco ded high alues o chlo ophyll A unde s ess condi ion, also eco ded high alues o chlo ophyll B. A highe alues o chlo ophyll con en helps he plan s o wi hs and wa e s ess h ough be e a ailabili ies o chlo ophyll, hese esul s a e in good way wi h hose ob ained by (Hamayun e al., 2010; Pi zad e al., 2011; Abdel-Kade e al., 2015a; Kannan e al., 2017). The highes alues we e eco ded by Giza 77 ollowed by Giza 68, 10229 and PimaS6 unde no mal condi ions, while Giza 77, Giza 68 and Giza 86 eco ded he highes alues unde s ess condi ions. I is in e es o no e ha geno ypes wi h high alues o ca o enoids concen a ion unde d ough condi ions eco ded high alues o chlo ophyll A o /and B. The dec emen o chlo ophyll con en unde d ough o wa e de ici s ess could be ela ed o pho o oxida ion esul ing om oxida i e s ess, which may be o ma ion o ee adicals, which lead o causes o chlo ophyll damage h ough he oxida i e, which educes pho osyn he ic p ocess. Unde s ess condi ions, ca o enoids and o he pigmen a ion may be abso p ion o he hu ul sub which o med as a esul 9 o ee adicals and a oid he chlo ophyll om damage. D ough s ess can also al e he issue concen a ions o chlo ophyll and ca o enoids. P oline is one o he osmop o ec an s o med in issues enable he plan o main ain low wa e po en ials ha allows addi ional wa e up ake om he s ess condi ion, hus bu e ing he immedia e e ec o wa e de ici . P oline accumula ion helps he plan s o minimize he dehyd a ion damage o he cell memb ane. The esul s showed ha , unde wa e s ess condi ions, he geno ypes showed signi ican dec eased in me abolic ac o s, such as dec eased in chlo ophyll (A, B), ca o enoids con en and enhanced accumula ion o p oline (Din e al., 2011; Kannan e al., 2017). Accumula ion o p oline is a widesp ead plan esponse o wa e de ici . P oline accumula ion is belie ed o play adap i e oles in plan s ess ole ance. Wa e de ici has a posi i e inc ease in he lea accumula ed p oline le els Table 6. The pa en al geno ypes showed signi icance di e ences in p oline accumula ed unde no mal and s ess condi ions. The pa en al geno ypes Giza 77 ollowed by Giza 68 and Giza 86 eco ded he highes alues o p oline accumula ed in lea unde s ess condi ions. On he o he side, pa en al geno ypes which showed high alues o p oline accumula ion unde no mal condi ions ga e he lowes alues o p oline unde s ess condi ions. Simila esul s we e ob ained by (Kannan e al., 2017). ABDEL-MONEAM, M.A. ET AL. ACTA AGRARIA DEBRECENIENSIS 2021-2 DOI: 10.34101/ACTAAGRAR/2/9194 10 Table 4. P opo ional con ibu ion o lines, es e s and hei in e ac ion o o al a iance o wa e ela ions and lea es chemical composi ion unde no mal and wa e s ess condi ions Sou ce To al wa e con en (%) F ee wa e (%) Bound wa e (%) Lea wa e de ici (%) Rela i e wa e con en (%) N S N S N S N S N S Lines 21.04 17.76 23.51 21.81 20.24 16.74 29.43 17.05 19.60 16.78 Tes e 35.59 13.31 12.38 5.26 40.59 14.67 12.43 14.22 22.06 11.96 Lines x Tes e 43.37 68.92 64.11 72.93 39.17 68.59 58.13 68.73 58.34 71.26 N = no mal i iga ion and S = wa e s ess Table 4. con inued Sou ce Osmo ic p essu e (ba ) Chlo ophyll A (mg g-1 dw ) Chlo ophyll B (mg g-1 dw ) Ca o enoides (mg g-1 dw ) P oline (mg g-1 w ) N S N S N S N S N S Lines 29.18 17.06 22.48 22.91 19.11 23.27 26.96 22.41 29.27 17.09 Tes e 12.75 14.28 6.58 6.79 6.71 1.63 8.11 11.84 12.36 14.20 Lines x Tes e 58.06 68.65 70.94 70.30 74.18 75.11 64.93 65.76 58.37 68.71 N = no mal i iga ion and S = wa e s ess Table 5. P opo ional con ibu ion o lines, es e s and hei in e ac ion o o al a iance o d ough ole ance indices unde no mal and wa e s ess condi ions Sou ce Tol M.P. H.M. S.S.I. G.M.P. S.T.I. Y.I. Y.S.I R.D.I D.I. Lines 43.50 12.88 11.55 42.83 12 42.79 13.78 42.79 42.82 25.21 Tes e 5.12 14.03 16.87 9.61 15.55 9.62 19.68 9.62 9.63 18 Lines x Tes e 51.38 73.09 71.58 47.56 72.45 47.59 66.54 47.59 47.56 56.78 N = no mal i iga ion and S = wa e s ess Di e en d ough ole ance indices we e calcula ed on he basis o yield o he geno ypes unde well i iga ed (Yp) and wa e de ici condi ions (Ys) as shown in Table 7. Da a e ealed ha g ea e he alue o ole ance index la ge he yield educ ion unde wa e de ici condi ions and highe he s ess sensi i i y. The geno ypes, pa en s and hyb ids we e signi ican ly di e ences. The pa en al geno ypes Giza 96 ollowed by Minu y and 10229 showed he highes educ ion in yield unde wa e de ici condi ions. The c oss combina ions which in ol ed Giza 96 as a common pa en eco ded highe alues o ole ance index. In he same ime, he c oss combina ion Minu y x Aus aly showed he highe alues o yield educ ion ollowed by he combina ions Giza 67 x Aus aly and Giza 69 x Aus aly. On he e e se end, s ess ole ance index (STI), which can be used o iden i y geno ypes ha p oduce high yield unde s ess and non- s ess condi ions. A la ge alue o s ess ole ance index (STI) show mo e ole an o s ess. O he yield-based es ima es o s ess esis ance a e mean p oduc i i y, geome ic mean and 10 ha monic mean. The geome ic mean is o en used by b eede s in e es ed in ela i e pe o mance since wa e de ici s ess can a y in se e i y in ield en i onmen o e ime. S ess sensi i e index (SSI) showed low amoun alues (less han uni y), indica ed high ole ance o geno ypes o wa e de ici (Zangi, 2005). To de e mine he mos desi able d ough ole ance c i e ia, co ela ion coe icien be ween yield in well i iga ion, yield in s ess and o he ole an indices we e calcula ed Table 8. The yield (Yn) unde well i iga ed condi ions ha e a s ong associa ion wi h (Ys) yield unde s ess condi ions and wi h mos o he ole an indices depic ing ha high yield po en ial unde well condi ions ga e supe io yield unde s ess condi ions and he loss o yield was ela i ely small. The e o e, indi ec selec ion o s ess en i onmen based on pe o mance o i iga ed condi ions would be e ec i e (Anwa e al., 2011). Da a illus a ed in Table 8 e ealed ha a la ge alue o ole an index show mo e sensi i i y o wa e de ici since co ela ion coe icien s be ween ole an index and o he yield indices we e nega i e and signi ican . Thus, a smalle alue o ole an index is a o ed o selec ion, based on ole an index a o geno ypes wi h low yield po en ial and high yield unde s ess condi ions. Simila esul s we e ob ained by (Zangi, 2005). ACTA AGRARIA DEBRECENIENSIS 2021-2 DOI: 10.34101/ACTAAGRAR/2/9194 11 Table 6. Mean pe o mance o co on geno ypes o wa e ela ions and lea es chemical composi ion unde no mal and wa e s ess condi ions Geno ypes To al wa e con en (%) F ee wa e (%) Bound wa e (%) Lea wa e de ici (%) Rela i e wa e con en (%) N S N S N S N S N S L1- Giza 45 77.77 67.69 10.17 9.21 67.6 58.48 10.33 8.74 69.85 60.82 L2- Minu y 77.4 63.78 10.1 8.68 67.3 55.1 10.07 8.06 68.98 56.84 L3- Giza 67 79.01 66.65 10.51 9.22 68.5 57.43 9.08 7.28 70.21 59.23 L4- Giza 68 79.54 65.82 10.6 9.12 68.95 56.7 8.17 6.5 70.1 58.01 L5- Giza 86 78.76 65.57 10.49 9.08 68.27 56.49 9.35 7.44 70.22 58.46 L6- Giza 77 79.02 66.32 10.55 9.2 68.47 57.12 8.4 6.74 70.32 59.02 L7- Giza 94 78.53 65.69 10.42 9.07 68.11 56.62 9.34 7.8 69.41 58.06 L8- Giza 96 76.05 65.17 9.37 8.38 66.68 56.79 11.37 8.77 66.95 57.37 L9- Giza 69 75.65 65.19 9.56 8.59 66.09 56.6 10.98 8.66 67.61 58.26 T1- Dand a 76.97 67.43 9.67 8.82 67.3 58.61 10.87 8.91 66.87 58.6 T2- Pima S6 77.72 66.49 10.22 9.09 67.5 57.4 10.08 8.16 69.58 59.53 T3- Aus aly 76.94 65.56 9.9 8.78 67.04 56.78 10.35 8.59 68.14 58.05 T4- 10229 78.45 65.93 10.28 8.99 68.18 56.94 10.01 8.33 69.81 58.66 Giza 45 x Dand a 83.13 69.34 11.21 9.1 71.92 60.24 8.76 10.68 75.72 61.4 Giza 45 x Pima S6 79.6 66.49 10.32 8.36 69.28 58.13 10.1 11.86 70.12 56.8 Giza 45 x Aus aly 84.3 66.89 11.36 8.76 72.94 58.13 9.32 11.69 76.74 59.14 Giza 45 x 10229 88.25 72.19 12.07 9.67 76.19 62.53 7.96 10.02 79.64 63.39 Minu y x Dand a 87.91 72.52 12.22 9.54 75.69 62.98 7.3 9.85 82.2 63.67 Minu y x Pima S6 85.63 69.93 11.59 9.23 74.04 60.7 8.36 10.59 77.61 61.63 Minu y x Aus aly 88.85 71.66 12.15 9.42 76.7 62.24 7.76 10.08 81.48 62.89 Minu y x 10229 86.28 70.34 11.84 9.43 74.44 60.91 8.62 10.53 77.75 61.63 Giza 67 x Dand a 87.75 72.29 12.1 9.67 75.65 62.63 7.33 9.99 79.54 63.27 Giza 67 x Pima S6 88.61 71.68 11.81 9.28 76.8 62.4 7.78 10.09 79.95 62.52 Giza 67 x Aus aly 88.27 71.02 12.26 9.49 76.01 61.53 7.99 10.29 80.75 62.14 Giza 67 x 10229 90.62 70.9 12.34 9.46 78.28 61.44 7.61 10.43 81.05 63.69 Giza 68 x Dand a 83.12 67.33 11.41 9 71.71 58.33 8.82 11.57 74.23 58.38 Giza 68 x Pima S6 88.51 70.79 11.93 9.33 76.58 61.46 7.84 10.41 79.75 62.03 Giza 68 x Aus aly 89.6 73.76 11.91 9.32 77.69 64.44 7.47 9.17 81.26 63.11 Giza 68 x 10229 88.07 70.56 11.23 8.74 76.84 61.82 7.85 10.33 78.5 61.15 Giza 86 x Dand a 83.85 68.17 11.5 9.11 72.35 59.06 8.54 11.16 75.72 64.48 Giza 86 x Pima S6 90.79 71.39 11.94 9.56 75.38 61.84 8.25 10.27 77.93 61.94 Giza 86 x Aus aly 85.78 69.5 11.58 9.18 74.2 60.32 8.75 10.7 77.76 61.25 Giza 86 x 10229 89.41 71.8 12.33 9.45 77.09 62.36 7.6 10.04 83.43 63.66 Giza 77 x Dand a 81.75 66.73 11.24 8.93 70.51 57.8 9.33 12.01 73.72 58.41 Giza 77 x Pima S6 89.54 73.2 12.26 9.81 77.28 63.39 7.55 9.62 80.79 64.29 Giza 77 x Aus aly 85.79 70.15 11.37 9.07 74.43 61.09 8.74 10.51 76.93 61.16 Giza 77 x 10229 90.6 71.33 12.19 9.38 78.42 61.95 7.65 10.15 81.74 62.61 Giza 94 x Dand a 88.02 73.29 12.46 9.78 75.56 63.51 7.31 9.39 81.7 63.81 Giza 94 x Pima S6 88.58 70.21 12.18 9.41 76.41 60.8 7.86 10.56 80.17 61.52 Giza 94 x Aus aly 86.28 69.63 11.63 9.14 74.65 60.49 8.57 10.66 77.87 61.09 Giza 94 x 10229 88.63 70.83 11.48 8.93 77.15 61.9 7.71 10.32 77.97 60.57 Giza 96 x Dand a 80.05 66.04 10.2 8.17 69.85 57.88 9.87 12.06 71.44 57.17 Giza 96 x Pima S6 84.33 67.39 11.53 8.97 72.8 58.43 9.35 11.62 75.52 58.6 Giza 96 x Aus aly 88.71 72.52 12.41 9.7 76.3 62.83 7.87 9.75 81.89 59.81 Giza 96 x 10229 87.32 71.68 12.27 9.47 78.53 62.21 7.58 10.11 82.24 63.2 Giza 69 x Dand a 81.09 67.16 10.59 8.52 70.51 58.64 9.47 11.51 73.46 59.07 Giza 69 x Pima S6 84.33 66.67 10.77 8.25 73.57 58.42 9.17 11.69 75.21 57.72 Giza 69 x Aus aly 89.13 71.62 12.29 9.59 76.84 62.03 7.69 10.14 80.15 62.16 Giza 69 x 10229 87.65 70.9 11.58 9.17 76.07 61.74 8.07 10.26 78.59 61.81 LSD (0.05) 1.73 1.49 0.35 0.24 1.52 1.33 0.497 0.53 2.48 1.77 ABDEL-MONEAM, M.A. ET AL. ACTA AGRARIA DEBRECENIENSIS 2021-2 DOI: 10.34101/ACTAAGRAR/2/9194 12 Table 6. con inued Geno ypes Osmo ic p essu e (ba ) Chlo ophyll A (mg g-1 dw ) Chlo ophyll B (mg g-1 dw ) Ca o enoides (mg g-1 dw ) P oline (mg g-1 w ) N S N S N S N S N S L1- Giza 45 4.72 3.81 3.2 3.01 1.28 1.12 1.44 1.33 353.45 665.76 L2- Minu y 4.81 3.98 3.18 2.99 1.27 1.15 1.39 1.34 355.99 675.36 L3- Giza 67 4.29 3.67 3.34 3.13 1.31 1.23 1.50 1.31 343.48 684.48 L4- Giza 68 4.14 3.37 3.51 3.07 1.33 1.24 1.56 1.37 341.61 685.33 L5- Giza 86 4.43 3.61 3.45 3.16 1.32 1.23 1.52 1.36 343.84 685.82 L6- Giza 77 4.18 3.37 3.56 3.09 1.34 1.24 1.57 1.37 343.51 684.66 L7- Giza 94 4.51 3.84 3.26 3.05 1.3 1.22 1.47 1.35 345.54 679.6 L8- Giza 96 5.7 4.78 3.01 2.93 1.37 1.15 1.38 1.26 363.67 683.18 L9- Giza 69 5.48 4.64 3.07 3.00 1.4 1.17 1.44 1.29 364.22 680.53 T1- Dand a 5.22 4.34 3.17 3.14 1.38 1.18 1.47 1.29 360.71 672.25 T2- Pima S6 4.71 4.1 3.39 3.1 1.41 1.21 1.55 1.35 353.06 676.8 T3- Aus aly 5.12 4.38 3.23 3.07 1.41 1.19 1.5 1.32 359.91 675.56 T4- 10229 4.57 4.04 3.44 3.06 1.42 1.22 1.57 1.36 350.26 675.04 Giza 45 x Dand a 3.62 5.36 3.59 3.04 1.39 1.2 1.6 1.35 327.84 564.34 Giza 45 x Pima S6 4.17 5.94 3.3 2.77 1.29 1.09 1.53 1.29 393.18 626.99 Giza 45 x Aus aly 3.85 5.86 3.37 2.85 1.35 1.16 1.5 1.27 357.44 618 Giza 45 x 10229 3.29 5.02 4.07 3.43 1.58 1.34 1.79 1.5 285.55 529.72 Minu y x Dand a 3.22 5.06 4.1 3.42 1.53 1.32 1.82 1.52 250.66 520.46 Minu y x Pima S6 3.46 5.31 3.73 3.15 1.44 1.24 1.67 1.41 306.7 559.85 Minu y x Aus aly 3.2 5.06 3.96 3.36 1.51 1.3 1.78 1.5 274.97 532.89 Minu y x 10229 3.56 5.28 3.85 3.26 1.53 1.3 1.7 1.44 320.71 556.41 Giza 67 x Dand a 3.03 5.01 4 3.36 1.5 1.31 1.77 1.48 252.25 527.87 Giza 67 x Pima S6 3.02 4.94 3.88 3.29 1.47 1.26 1.73 1.47 276.3 533.15 Giza 67 x Aus aly 3.31 5.16 3.85 3.26 1.5 1.28 1.69 1.44 287.4 543.72 Giza 67 x 10229 3.15 5.22 3.93 3.28 1.52 1.3 1.72 1.44 267.04 551.13 Giza 68 x Dand a 3.65 5.79 3.6 3.02 1.42 1.24 1.57 1.31 331.28 611.4 Giza 68 x Pima S6 3.09 4.6 3.86 3.26 1.48 1.28 1.7 1.44 279.21 550.07 Giza 68 x Aus aly 3.24 5.21 3.97 3.31 1.45 1.22 1.85 1.54 280 545.84 Giza 68 x 10229 3.24 5.18 3.68 3.09 1.36 1.15 1.7 1.42 259.91 484.52 Giza 86 x Dand a 3.53 5.59 3.67 3.1 1.43 1.26 1.61 1.36 316.48 589.72 Giza 86 x Pima S6 3.41 5.15 3.96 3.34 1.56 1.32 1.72 1.45 300.88 542.67 Giza 86 x Aus aly 3.62 5.36 3.68 3.11 1.44 1.23 1.66 1.4 327.58 565.4 Giza 86 x 10229 3.15 5.03 3.84 3.22 1.46 1.24 1.72 1.44 266.78 530.51 Giza 77 x Dand a 3.19 5.08 3.51 2.98 1.39 1.23 1.56 1.32 358.24 634.65 Giza 77 x Pima S6 3.13 4.83 4.14 3.5 1.59 1.36 1.82 1.54 378.79 637.3 Giza 77 x Aus aly 3.62 5.27 3.77 3.17 1.46 1.23 1.68 1.42 327.05 555.62 Giza 77 x 10229 3.15 5.09 3.93 3.33 1.51 1.3 1.75 1.48 269.16 536.33 Giza 94 x Dand a 3.02 4.71 4.11 3.48 1.55 1.35 1.78 1.52 251.19 496.15 Giza 94 x Pima S6 3.26 5.29 3.89 3.25 1.51 1.3 1.72 1.43 280.53 558.26 Giza 94 x Aus aly 3.54 5.34 3.79 3.2 1.48 1.27 1.69 1.42 318.06 563.29 Giza 94 x 10229 3.19 5.17 3.64 3.09 1.35 1.17 1.69 1.43 272.33 545.31 Giza 96 x Dand a 4.08 6.04 3.3 2.76 1.27 1.08 1.51 1.26 264.14 508.57 Giza 96 x Pima S6 3.86 5.82 3.58 3.01 1.45 1.24 1.57 1.32 359.3 614.3 Giza 96 x Aus aly 3.25 4.89 3.98 3.38 1.54 1.32 1.75 1.48 280.79 515.44 Giza 96 x 10229 3.12 5.06 3.88 3.28 1.49 1.27 1.73 1.47 265.46 534.21 Giza 69 x Dand a 3.92 5.76 3.45 2.92 1.32 1.14 1.61 1.35 365.38 608.22 Giza 69 x Pima S6 3.79 5.85 3.34 2.8 1.29 1.1 1.53 1.28 349.51 617.74 Giza 69 x Aus aly 3.86 6.01 3.97 3.35 1.54 1.32 1.73 1.46 271.54 536.06 Giza 69 x 10229 3.34 5.14 3.87 3.23 1.48 1.25 1.6 1.46 291.63 564.34 LSD (0.05) 0.18 0.25 0.13 0.11 0.04 0.03 0.06 0.05 22.25 24.47 LSD (0.01) 0.24 0.33 0.18 0.14 0.05 0.04 0.08 0.07 29.55 32.50 ACTA AGRARIA DEBRECENIENSIS 2021-2 DOI: 10.34101/ACTAAGRAR/2/9194 13 Table 7. Mean alues o d ough ole ance indices in co on Pa en s Tol M.P. H.M. S.S.I. G.M.P S.T.I. Y.I. Y.S.I R.D.I D.I. L1- Giza 45 18.73 68.23 66.79 1.02 67.5 0.76 0.9 0.76 0.99 0.69 L2- Minu y 28.53 68.19 65.21 1.49 66.68 0.65 0.82 0.65 0.85 0.54 L3- Giza 67 11.35 84 83.61 0.55 83.8 0.87 1.19 0.87 1.14 1.04 L4- Giza 68 14.99 77.02 76.26 0.77 76.64 0.82 1.06 0.82 1.07 0.87 L5- Giza 86 19.15 67.03 65.66 1.08 66.35 0.75 0.88 0.75 0.98 0.66 L6- Giza 77 17.82 65.22 63.86 1.03 64.53 0.76 0.86 0.76 0.99 0.66 L7- Giza 94 11.53 78.34 77.91 0.59 78.13 0.86 1.11 0.86 1.12 0.96 L8- Giza 96 53.22 71.49 61.57 2.34 66.34 0.46 0.68 0.46 0.6 0.31 L9- Giza 69 15.83 67.36 66.33 0.92 66.84 0.79 0.91 0.79 1.03 0.72 T1- Dand a 13.59 59.4 58.6 0.89 59 0.79 0.8 0.79 1.03 0.64 T2- Pima S6 15.94 72.69 71.79 0.86 72.24 0.8 0.99 0.8 1.04 0.79 T3- Aus aly 15.47 62.89 61.92 0.93 62.41 0.78 0.84 0.78 1.02 0.66 T4- 10229 24.26 34.97 30.71 2.2 32.77 0.49 0.35 0.49 0.64 0.17 Giza 45 x Dand a 18.96 80.38 79.24 0.91 79.8 0.79 1.08 0.79 1.03 0.85 Giza 45 x Pima S6 11.82 83.51 83.09 0.57 83.3 0.87 1.18 0.87 1.13 1.03 Giza 45 x Aus aly 12.57 71.42 70.86 0.7 71.14 0.84 0.99 0.84 1.09 0.83 Giza 45 x 10229 18.57 64.73 63.14 1.05 63.92 0.76 0.85 0.76 0.98 0.64 Minu y x Dand a 20.16 77.99 76.53 0.97 77.26 0.77 1.04 0.77 1.01 0.81 Minu y x Pima S6 24.61 77.34 75.36 1.19 76.35 0.72 0.99 0.72 0.94 0.72 Minu y x Aus aly 56.2 87.57 78.23 2.08 82.74 0.52 0.91 0.52 0.67 0.47 Minu y x 10229 23.91 81.95 80.1 1.07 81.01 0.75 1.07 0.75 0.98 0.8 Giza 67 x Dand a 11.83 69.08 68.57 0.69 68.82 0.84 0.96 0.84 1.09 0.81 Giza 67 x Pima S6 17.42 74.15 73.06 0.9 73.6 0.79 1 0.79 1.03 0.79 Giza 67 x Aus aly 39.07 91.41 87.07 1.51 89.21 0.65 1.1 0.65 0.85 0.72 Giza 67 x 10229 12.95 73.05 72.45 0.71 72.75 0.84 1.02 0.84 1.09 0.85 Giza 68 x Dand a 24.2 81.78 79.98 1.11 80.87 0.74 1.06 0.74 0.97 0.79 Giza 68 x Pima S6 24.97 66.61 64.25 1.35 65.42 0.69 0.83 0.69 0.89 0.57 Giza 68 x Aus aly 32.01 72.04 68.48 1.57 70.24 0.64 0.85 0.64 0.83 0.54 Giza 68 x 10229 18.11 80.58 79.47 0.86 80.02 0.8 1.09 0.8 1.04 0.87 Giza 86 x Dand a 23.2 110.79 109.55 0.83 110.17 0.81 1.51 0.81 1.05 1.23 Giza 86 x Pima S6 26.69 70.91 68.26 1.37 69.57 0.68 0.88 0.68 0.89 0.61 Giza 86 x Aus aly 21.75 84.45 82.91 0.98 83.67 0.77 1.12 0.77 1.01 0.87 Giza 86 x 10229 19.51 73.61 72.27 1 72.93 0.77 0.97 0.77 1 0.75 Giza 77 x Dand a 12.25 79.69 79.2 0.61 79.44 0.86 1.12 0.86 1.12 0.96 Giza 77 x Pima S6 11.75 87.12 86.72 0.54 86.92 0.87 1.24 0.87 1.14 1.08 Giza 77 x Aus aly 20.07 78.38 76.94 0.97 77.66 0.77 1.04 0.77 1.01 0.81 Giza 77 x 10229 20.62 57.57 55.54 1.27 56.54 0.71 0.72 0.71 0.92 0.51 Giza 94 x Dand a 15.72 77.04 76.22 0.8 76.63 0.81 1.06 0.81 1.06 0.86 Giza 94 x Pima S6 13.26 72.02 71.4 0.72 71.71 0.83 1 0.83 1.08 0.83 Giza 94 x Aus aly 27.7 74.14 71.31 1.33 72.71 0.69 0.92 0.69 0.9 0.64 Giza 94 x 10229 19.02 77.6 76.37 0.92 76.98 0.79 1.04 0.79 1.02 0.81 Giza 96 x Dand a 40 93.83 89.55 1.52 91.66 0.65 1.13 0.65 0.84 0.73 Giza 96 x Pima S6 23.87 66.59 64.43 1.3 65.5 0.7 0.83 0.7 0.91 0.58 Giza 96 x Aus aly 32.16 72.04 68.41 1.57 70.2 0.64 0.85 0.64 0.83 0.54 Giza 96 x 10229 36.17 71.06 66.43 1.75 68.7 0.59 0.81 0.59 0.77 0.48 Giza 69 x Dand a 14.27 64.43 63.59 0.85 64.01 0.8 0.87 0.8 1.05 0.7 Giza 69 x Pima S6 15.26 87.52 86.83 0.68 87.18 0.84 1.22 0.84 1.1 1.02 Giza 69 x Aus aly 37.78 69.53 64.29 1.83 66.85 0.58 0.77 0.58 0.75 0.45 Giza 69 x 10229 16.26 66.17 65.14 0.92 65.65 0.79 0.89 0.79 1.02 0.69 LSD (0.05) 9.47 10.81 10.58 0.37 10.66 0.09 0.16 0.09 0.11 0.17