Diversity of spiders in Daba Mountain National Nature Reserve, Chongqing, China (II): Two new species of Hahniidae (Arachnida, Araneae)

217 Diversity of spiders in Daba Mountain National Nature Reserve, Chongqing, China (II): Two new species of Hahniidae (Arachnida, Araneae) Yu-Jun Cai1, Lu-Yu Wang1,2 , Ben-Chao Zhu3, Wei Lu3, Zhi-Sheng Zhang1 1 Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Sciences, Southwest University, Chongqing 400715, China 2 Chongqing Urban Ecosystem Positioning Observation and Research Station, Chongqing 400036, China 3 Management Center of Daba Mountain National Nature Reserve, Chongqing 405909, China Corresponding author: Zhi-Sheng Zhang ([email protected]) Copyright: © Yu-Jun Cai et al. This is an open access article distributed under terms of the Creative Commons Attribution License (Attribution 4.0 International – CC BY 4.0). Research Article Abstract Two new species of Hahniidae, Hahnia dabashan Cai, Wang & Zhang, sp. nov. (♂) and Sinahahnia chengkou Cai, Wang & Zhang, sp. nov. (♂♀), are described and illustrated with photographs and drawings, based on material from Daba Mountain National Nature Reserve in Chongqing, southwestern China. Key words: Comb-tailed spiders, description, Hahnia, morphology, Sinahahnia, taxonomy Introduction The spider family Hahniidae is relatively small and almost globally distributed, including 242 extant and 16 fossil species, belonging to 29 extant and four fossil genera, with the highest diversity in Europe and Asia (WSC 2025). In China, 48 species in nine genera are known, with most records confined to southwest regions (WSC 2025). Among these, nine species comprising Hahnia (4), Neoantistea (3) and Sinahahnia (2) are known from Chongqing Municipality (Wang et al. 2010; Zhang and Zhang 2013; Liu et al. 2015; Wang and Zhang 2024; Zheng et al. 2025; Wang and Zhang 2025). This study is part of a series dealing with the diversity of spiders from Daba Mountain National Nature Reserve (MNNR). Following the description of three new species of Cicurina Menge, 1871 (Cicurinidae) (Wang et al. 2024), the present paper describes two new species of Hahniidae from the Reserve. Material and methods Specimens were preserved in 75% ethanol and examined, illustrated, photographed and measured using a Leica M205A stereomicroscope equipped with a drawing tube, a Leica DFC450 camera and Leica Application Suite software (ver. 4.6). Male palps and epigynes were examined and illustrated after dissection. Epigynes were cleared in pancreatin (Álvarez-Padilla and Hormiga 2007). Academic editor: Yuri Marusik Received: 3 April 2025 Accepted: 25 August 2025 Published: 2 October 2025 ZooBank: https://zoobank. org/19BAE6A9-3F31-4689-AE7A5ECC72E84A87 Citation: Cai Y-J, Wang L-Y, Zhu B-C, Lu W, Zhang Z-S (2025) Diversity of spiders in Daba Mountain National Nature Reserve, Chongqing, China (II): Two new species of Hahniidae (Arachnida, Araneae). ZooKeys 1254: 217–224. https://doi.org/10.3897/ zookeys.1254.154722 ZooKeys 1254: 217–224 (2025) DOI: 10.3897/zookeys.1254.154722 218 ZooKeys 1254: 217–224 (2025), DOI: 10.3897/zookeys.1254.154722 Yu-Jun Cai et al.: Four hahniid spiders from Daba Maountain, China Eye sizes were measured as the maximum dorsal diameter. Prosoma and opisthosoma are measured dorsally. Leg measurements are given as: total length (femur, patella+tibia, metatarsus, tarsus). All measurements are in millimetres. The depository for the material is the Collection of Spiders, School of Life Sciences, Southwest University, Chongqing, China (SWUC). Median apophysis identified here originating from the haematodochal area, follows Burgo et al. (2025), Rubio et al. (2014), Dupérré and Tapia (2024) etc., rather than ‘conductor’ of Gertsch (1934), Bosmans (1992) and Chu et al. (2023). Abbreviations used in the text: ALE, anterior lateral eye; AME, anterior median eye; MOA, median ocular area; PLE, posterior lateral eye; PME, posterior median eye. Taxonomy Family Hahniidae Bertkau, 1878 Genus Hahnia C. L. Koch, 1841 Type species. Hahnia pusilla C.L. Koch, 1841. Composition. In total, the genus comprises 105 species, among which 26 species are known from China, and four are known from Chongqing: H. subcorticicola Liu, Huang & Zhang, 2015, H. thorntoni Brignoli, 1982, H. zhejiangensis Song & Zheng, 1982 and H. zhui Zhang & Chen, 2015 (Wang et al. 2010; Zhang and Zhang 2013; Liu et al. 2015; Zhang and Chen 2015). Here, the fifth Hahnia species from Chongqing is described. Hahnia dabashan Cai, Wang & Zhang, sp. nov. https://zoobank.org/782830A5-9C32-4B69-A987-158300BDD3DF Figs 1A–C, 2A–D Type material. Holotype • ♂ (SWUC-T-HA-16-01), China, Chongqing Munic., Chengkou Co., Daba MNNR, Longtian Town, Wuli Vill., 32°04.269'N, 108°39.914'E, elev. 1286 m, 16.03.2018, Z.S. Zhang et al. leg. Paratypes • 4♂ (SWUC-T-HA-16-02~05), same data as for the holotype. Etymology. The specific name is derived from the type locality (Dabashan = Daba Mountain); noun in apposition. Diagnosis. The male palp of the new species is similar to that of H. corticicola Bösenberg & Strand, 1906 (Liu et al. 2015: 297, fig. 3A–I) in the general shape of the cymbium, embolus, tegulum, and retrolateral tibial apophysis (RTA). It can be distinguished by the following combination of characters: a thorn-like process (TF) on the proximal femur (vs. absent); a flake-like patellar apophysis with a serrated margin (vs. rod-shaped with a hooked tip); and indistinct teeth on the distal retrolateral tibial apophysis (vs. distinct teeth) (Figs 1A–C, 2B–D). Description. Male holotype (Fig. 2A) total length 1.55. Prosoma 0.78 long, 0.62 wide; opisthosoma 0.84 long, 0.61 wide. Carapace yellowish brown, with few black setae. Eye sizes and interdistances: AME 0.02, ALE 0.05, PME 0.05, PLE 0.0.05; AME–AME 0.02, AME–ALE 0.02, PME–PME 0.05, PME–PLE 0.02, ALE–PLE 0.01. MOA 0.13 long, front width 0.06, back width 0.13. Clypeus 219 ZooKeys 1254: 217–224 (2025), DOI: 10.3897/zookeys.1254.154722 Yu-Jun Cai et al.: Four hahniid spiders from Daba Maountain, China Figure 1. Hahnia dabashan sp. nov., male holotype (A, C) and paratype (B). A. Left male palp, ventral view; B. Same, retrolateral view; C. Same, dorsal view. Abbreviations: CF = cymbial furrow; Em = embolus; MA = median apophysis; PA = patellar apophysis; RTA = retrolateral tibial apophysis; T = tegulum. Figure 2. Hahnia dabashan sp. nov., male holotype (A, B, D) and paratype (C). A. Male habitus, dorsal view; B. Left male palp, ventral view; C. Same, retrolateral view; D. Same, dorsal view. height 0.08. Chelicerae yellowish brown, with 2 promarginal and 4 retromarginal teeth. Endites yellowish brown. Labium yellowish brown, wider than long. Leg measurements: I 1.63 (0.50, 0.56, 0.31, 0.26); II 1.55 (0.47, 0.50, 0.31, 0.27); III 1.35 (0.37, 0.45, 0.27, 0.26); IV 1.83 (0.50, 0.64, 0.38, 0.31). Opisthosoma oval, dorsum yellowish brown, dorsally with 3 light chevrons, venter yellowish brown. Palp (Figs 1A–C, 2B–D). Femur proximal end with thorn-like outgrowth; patellar apophysis (PA) flake-like, 3 times longer than wide in retrolateral view; distal part of retrolateral tibial apophysis (RTA) extending dorsally then proximally; cymbium c. 1.7 times longer than wide, with deep, long (as long as cymbium) and semi-transparent retrolateral furrow; embolus originating mid-retrolaterally (c. 3 o’clock position), clockwise curved along cymbial margin, then inside 220 ZooKeys 1254: 217–224 (2025), DOI: 10.3897/zookeys.1254.154722 Yu-Jun Cai et al.: Four hahniid spiders from Daba Maountain, China cymbial furrow in the left male palp; tip of embolus embedded in a transparent pocket. Median apophysis (MA) located near base of embolus. Female unknown. Variation. Males (N = 5) total length 1.55–1.75. Distribution. Known only from the type locality in Chongqing, China. Genus Sinahahnia Wang & Zhang, 2024 Type species. Sinahahnia eyu Wang & Zhang, 2024. Composition. Three species, all described from China, with two from Chongqing: S. eyu Wang & Zhang, 2024 and S. yintiaoling Wang & Zhang, 2024 (Wang and Zhang 2024). Here, the third Sinahahnia species from Chongqing is described. Sinahahnia chengkou Cai, Wang & Zhang, sp. nov. https://zoobank.org/6EC7A5EA-3B6F-46F9-B1C3-CA169719439A Figs 3A–E, 4A–G Type material. Holotype • ♂ (SWUC-T-HA-17-01), China, Chongqing Munic., Chengkou Co., Daba MNNR, Longtian Town, Wuli Vill., 32°04.269'N, 108°39.914'E, elev. 1286 m, 16.03.2018, Z.S. Zhang et al. leg. Paratypes • 2♂9♀ (SWUC-T-HA-17-02~12), same data as holotype • 1♂ (SWUC-T-HA-17-13), Wuli Vill., 32°03.726'N, 108°40.351'E, elev. 1206 m, 16.03.2018, Z.S. Zhang et al. leg. • 1♂2♀ (SWUC-T-HA-17-14~16), Wuli Vill., 32°04.443'N, 108°39.278'E, elev. 1264 m, 16.09.2012, L.Y. Wang, X.K. Jiang leg. • 2♀ (SWUC-T-HA-17-17~18), Xiuqi Town, Xumu Vill., 31°54.484'N, 109°03.556'E, elev. 1670 m, 27.03.2013, X.K. Jiang and X.W. Meng leg. Etymology. The specific name is derived from the type locality; noun in apposition. Diagnosis. The new species resembles S. eyu (Wang and Zhang 2024: 251, figs 2, 3C–G, 4B, C) in having a bifurcated patellar apophysis, long, slender embolus, large, membranous median apophysis, and long, spiral copulatory ducts, but differs from the latter by the curved retrolateral tibial apophysis (vs twisted with small thorns), the finger-shaped retrolateral patellar apophysis (vs absent; Figs 3A–C, 4C–E), and cordate spermathecae (vs reniform; Figs 3E, 4G). Description. Male holotype (Fig. 4A) total length 2.15. Prosoma 1.01 long, 0.80 wide; opisthosoma 1.18 long, 0.84 wide. Carapace yellowish brown. Fovea longitudinal. Eye sizes and interdistances: AME 0.02, ALE 0.07, PME 0.07, PLE 0.09; AME–AME 0.02, AME–ALE 0.01, PME–PME 0.04, PME–PLE 0.02, ALE– PLE 0.01. MOA 0.16 long, front width 0.06, back width 0.19. Clypeus height 0.12. Chelicerae yellowish brown, with 2 promarginal and 4 retromarginal teeth. Endites yellowish brown. Labium yellowish brown. Sternum yellowish brown. Leg measurements: I 2.85 (0.81, 0.91, 0.60, 0.53); II 2.61 (0.77, 0.83, 0.53, 0.48); III 2.08 (0.70, 0.49, 0.44, 0.45); IV 3.07 (0.89, 0.93, 0.72, 0.53). Opisthosoma oval, dorsum yellowish brown, dorsally with 6 light chevrons, venter yellowish brown. Palp (Figs 3A–C, 4C–E). Femur unmodified. Patella with 2 apophyses − proximal and distal: proximal one (PA) curved, short, with 2 tips, one of them twice larger than other; distal apophysis (DPA) finger-shaped. Tibia slightly shorter 221 ZooKeys 1254: 217–224 (2025), DOI: 10.3897/zookeys.1254.154722 Yu-Jun Cai et al.: Four hahniid spiders from Daba Maountain, China Figure 3. Sinahahnia chengkou sp. nov., male holotype (A–C) and female paratype (D–E). A. Left male palp, ventral view; B. Same, retrolateral view; C. Same, dorsal view; D. Epigyne, ventral view; E. Same, dorsal view. Abbreviations: CD = copulatory duct; CF = cymbial furrow; CO = copulatory opening; Em = embolus; FD = fertilization duct; LTA = lateral tibial apophysis; MA = median apophysis; PA = patellar apophysis; RPA = retrolateral patellar apophysis; RTA = retrolateral tibial apophysis; Sp = spermatheca; Spp = spermathecal protuberance; Ss = secondary spermatheca; T = tegulum. than patella, with 2 apophyses: retrolateral apophysis (RTA) curved retrolaterally, with intumescent base and spine-like tip; lateral apophysis (LTA) with blunt end, slightly wider than tall in dorsal view. Cymbial furrow (CF) almost as long as cymbium. Tegulum longer than wide. Embolus with wide embolic base, originating at 6-o’clock position, silk-like, curved clockwise (in left palp) as whole circle, its tip staying inside of cymbial furrow near embolic base. Distal part of median apophysis (MA) almost 7 times wider than its base; sperm duct distinct in tegulum and embolic base, curved inside of embolic base. Female paratypes total length 2.00–2.47. Paratype (SWUC-T-HA-09-02, Fig. 4B) total length 2.13. Prosoma 0.87 long, 0.63 wide; opisthosoma 1.26 long, 0.95 wide. Carapace yellowish brown. Eye sizes and interdistances: AME 0.03, ALE 0.07, PME 0.07, PLE 0.08; AME–AME 0.02, AME–ALE 0.01, PME–PME 0.05, PME–PLE 0.02, ALE–PLE 0.01. MOA 0.14 long, front width 0.07, back width 0.18. Clypeus height 0.08. Leg measurements: I 11.38 (3.09, 4.21, 2.50, 1.58); II 10.85 (3.04, 3.83, 2.43, 1.55); III 11.19 (2.95, 3.62, 3.09, 1.53); IV 16.17 (3.90, 5.11, 5.10, 2.06). 222 ZooKeys 1254: 217–224 (2025), DOI: 10.3897/zookeys.1254.154722 Yu-Jun Cai et al.: Four hahniid spiders from Daba Maountain, China Figure 4. Sinahahnia chengkou sp. nov. male holotype (A, C–E) and female paratype (B, F, G). A. Male habitus, dorsal view; B. Female habitus, dorsal view; C. Left male palp, ventral view; D. Same, retrolateral view; E. Same, dorsal view; F. Epigyne, ventral view; G. Same, dorsal view. Epigyne (Figs 3D–E, 4F–G). Plate as long as wide; copulatory opening (CO) small located slightly mid-anteriorly; copulatory duct (CD) thin, long, wrapped 3 times around secondary spermathecae (Ss); secondary spermatheca small, located anteriorly; spermatheca (Sp) about 1.5 times longer than wide, folded in half, as large as secondary spermatheca, with a small protuberance 223 ZooKeys 1254: 217–224 (2025), DOI: 10.3897/zookeys.1254.154722 Yu-Jun Cai et al.: Four hahniid spiders from Daba Maountain, China antero-laterally (Spp); spermathecae spaced slightly wider by one width, but narrower than the length of it. Variation. Males (N = 5) total length 2.08–2.22, females (N = 13) total length 2.00–2.47. Distribution. Known only from the type locality in Chongqing, China. Acknowledgements Many thanks are given to the subject editor, Yuri M. Marusik and the reviewer, Alireza Zamani for their helpful comments. And thanks are also given to Mr Xuan-Kong Jiang, Xiang-Wei Meng and Zhi-Sun Wu (SWUC previously) for their assistance during the fieldwork and collection. Additional information Conflict of interest The authors have declared that no competing interests exist. Ethical statement No ethical statement was reported. Use of AI No use of AI was reported. Funding This research was supported by the Scientific investigation project of biological resource background of Chongqing Daba Mountain National Nature Reserve. Author contributions All authors have contributed equally. Author ORCIDs Yu-Jun Cai https://orcid.org/0009-0003-8259-6783 Lu-Yu Wang https://orcid.org/0000-0002-5250-3473 Ben-Chao Zhu https://orcid.org/0009-0007-1235-8308 Wei Lu https://orcid.org/0009-0005-3504-3777 Zhi-Sheng Zhang https://orcid.org/0000-0002-9304-1789 Data availability All of the data that support the findings of this study are available in the main text. 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