Supplementary material 1 from: Matos M, Teixeira A, Nogueira AB, Padilha J, Sousa R (2025) Evaluating brown trout as a potential biological control agent of signal crayfish. In: Anastácio P, Ribeiro F, Chainho P (Eds) Invasions in Aquatic Systems. NeoBiota 102: 109-123. https://doi.org/10.3897/neobiota.102.152018

1 Table S1 Physical-chemical characterization of all the sampling sites and sampling Julian days. Day 107 Day 140 B1 B2 B3 B4 B5 B1 B2 B3 B4 B5 Temperature (°C) 11.2 11.4 11.5 10.3 10.1 12.1 12.2 12.4 10.9 10.2 Oxygen (mg/L) 10.82 10.81 10.23 9.96 10.07 10.79 10.74 10.42 10.38 10.41 Conductivity (μS/cm) 54.00 52.10 51.50 21.79 10.00 54.20 54.20 53.20 24.56 10.72 TDS (mg/L) 34.70 33.00 32.50 13.46 6.14 36.70 35.10 33.12 14.02 6.95 pH 7.38 7.35 7.34 7.23 7.20 7.33 7.32 7.30 7.24 7.23 Day 166 Day 188 B1 B2 B3 B4 B5 B1 B2 B3 B4 B5 Temperature (°C) 15.0 14.1 14.0 14.0 13.0 16.7 16.5 16.6 16.3 16.2 Oxygen (mg/L) 9.47 10.08 9.66 9.94 10.12 9.78 9.49 9.54 9.10 9.31 Conductivity μS/cm) 51.10 47.60 47.30 19.85 9.18 71.90 69.40 69.30 25.40 12.30 TDS (mg/L) 29.8 28.4 28.4 11.76 5.57 41.6 39.4 39.2 13.84 6.92 pH 7.40 7.38 7.31 7.43 7.32 7.40 7.37 7.34 7.28 7.25 Day 208 Day 226 B1 B2 B3 B4 B5 B1 B2 B3 B4 B5 Temperature (°C) 19.1 18.5 18.0 16.7 15.0 21.0 20.3 19.5 16.4 15.8 Oxygen (mg/L) 9.25 9.36 9.22 9.40 9.30 8.98 8.20 7.91 7.86 8.85 Conductivity (μS/cm) 94.9 91.10 88.70 29.50 13.82 116.60 110.20 108.70 64.30 18.56 TDS (mg/L) 51.00 49.50 48.80 16.57 8.03 70.20 65.70 57.70 37.70 12.10 pH 7.38 7.36 7.34 7.29 7.23 7.44 7.34 7.29 7.27 7.25 Day 243 Day 279 B1 B2 B3 B4 B5 B1 B2 B3 B4 B5 Temperature (°C) 20.2 20.0 19.2 16.2 15.7 15.4 14.3 13.9 13.4 13.3 Oxygen (mg/L) 8.95 8.44 8.2 8.13 9.01 9.97 9.93 9.71 9.39 9.00 Conductivity (μS/cm) 120.60 113.40 98.70 61.20 18.31 96.90 90.90 88.90 34.50 16.59 TDS (mg/L) 78.2 75.70 67.70 35.40 12.70 57.20 54.70 53.80 20.90 10.12 pH 7.49 7.38 7.27 7.25 7.23 7.44 7.36 7.34 7.26 7.25 2 Day 347 B1 B2 B3 B4 B5 Temperature (°C) 4.6 4.4 4.5 4.4 4.3 Oxygen (mg/L) 12.41 12.54 12.48 12.33 12.59 Conductivity (μS/cm) 37.7 35.6 34.91 16.69 8.07 TDS (mg/L) 28.9 27.3 26.8 12.58 5.98 pH 7.06 7.06 7.05 7.27 7.32 Table S2 Species Count in Trout Stomach Content Figure S1. Residual diagnostics of the generalized linear model (GLM) with a binomial distribution fitted to assess the abiotic and biotic factors influencing crayfish predation by brown trout. The plots include: (A) Residuals vs. Fitted Values – Checking for systematic patterns that might indicate model misspecification. (B) Normal Q-Q Plot – Assessing the normality of standardized deviance residuals. (C) Scale-Location Plot – Evaluating homoscedasticity (constant variance of residuals). (D) Residuals vs. Leverage Plot – Identifying influential observations using Cook's distance.