Supplementary material 2 from: Reinhart T, Espinosa Prieto A, Begoc T, Tinguy H, Bick F, Chanez E, Beisel J-N, Hardion L (2025) A comparative analysis of hybridisation capture and PCR-based eDNA metabarcoding for monitoring bryophytes in riparian ecosystems. Metabarcoding and Metagenomics 9: e154548. https://doi.org/10.3897/mbmg.9.154548

Supplementary material for: A comparative analysis of hybridisation capture and PCR-based eDNA metabarcoding for monitoring bryophytes in riparian ecosystems Figure S1: Visualisations of the DNA logoplot of the designed ITS2 primer pair BraF_BraR, showing where to add degenerate nucleotides to maintain universality among bryophytes (1175 sequences for Marchantiophyta, 108 Dicraniadae, 108 Pleurocarp and 14 other Bryophyta) or vascular plants (6797 sequences) and improve PCR results. The number of mismatches for each taxa for the forward (x-axis) and reverse (y-axis) are shown in pie charts. 1 Figure S2. Barcode characteristics of each ITS2 primer pair tested in in silico PCR. The mean amplicon length (bp) is displayed for each primer pair. Barcode coverage (BC) and barcode specificity (Bs) are presented as percentages.