QUALITY CONTROL AND STANDARDIZATION OF BIOACTIVE COMPOUNDS IN THE DRY EXTRACT 'EXTRADENT'

305 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz QUALITY CONTROL AND STANDARDIZATION OF BIOACTIVE COMPOUNDS IN THE DRY EXTRACT 'EXTRADENT' Yuldasheva Sh.S. Akhmadjonova D.A. 'Alfraganus University, Tashkent city, Republic of Uzbekistan Andijan State Medical Institute, Andijan city, Republic of Uzbekistan e-mail: [email protected] https://doi.org/10.5281/zenodo.17335244 Relevance: Herbal preparations remain an important direction in pharmaceutical science due to their wide pharmacological effects and relatively low toxicity. 'Extradent' is a dry extract consisting of Calendula officinalis, Urtica dioica, Capsella bursa-pastoris, and Polygonum hydropiper. These plants are known to contain flavonoids, carotenoids, tannins, amino acids, and mineral elements that contribute to the therapeutic properties of the preparation. Keywords: Extradent, quality control, standardization, rutin, flavonoids, HPLC, UVspectrophotometry, carotenoids, tannins, amino acids, minerals. Purpose of the research.To ensure quality control and standardization of the dry extract 'Extradent' by identifying its main biologically active substances, with special attention to flavonoids. Methods.High-performance liquid chromatography (HPLC) and UV-spectrophotometry were applied. The extract was dissolved in 70% ethanol and filtered. Chromatographic analysis was carried out on a C18 column with acetonitrile, water, and acetic acid as the mobile phase. UVspectrophotometry was used to measure the total flavonoid content using the AlCl₃ complex method. Results .The chromatogram revealed several phenolic compounds, with rutin identified as the main marker. Its clear peak confirmed its suitability for standardization. Spectrophotometric analysis showed a significant presence of flavonoids beyond rutin, indicating the complex composition of the extract. Literature data confirm that carotenoids, tannins, amino acids, and minerals also strengthen the therapeutic potential of 'Extradent'. Conclusion. The combination of HPLC and UV-spectrophotometry is an effective approach for the quality control of 'Extradent'. Rutin can be used as a reliable marker, while the presence of other biologically active compounds ensures a wide spectrum of pharmacological activity. The results provide a basis for certification and broader medical application of the preparation.