DETERMINATION OF THE AMOUNT OF OXIDOLIC ACIDS CONTAINED IN THE "SAFROBOS" COLLECTION

418 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz DETERMINATION OF THE AMOUNT OF OXIDOLIC ACIDS CONTAINED IN THE "SAFROBOS" COLLECTION Olimov H.K. Mirrakhimova T.A. Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan e-mail: [email protected], tel: +998903259258 https://doi.org/10.5281/zenodo.17341172 Introduction Cholear agents based on medicinal plants are in high demand in clinical practice, as their effect is milder and longer-lasting compared to synthetic drugs. In order to increase the choleretic properties of plant-derived medicines and treat concomitant diseases, various plant-based preparations are used. Medicinal products obtained on the basis of natural raw materials, compared to synthetic medicinal products, have a wide range of pharmacological effects, are cheaper in price, have fewer or no adverse effects on the body. Research objective Determination of the amount of the sum of oxidoric acids in the "Safroboz" collection using the SF method Materials and methods Optical density of solutions Spectrophotometer UVShimadzu-1800 Japan 329±2 nm length was measured (chlorogenic acid). 50% ethyl alcohol was used as a comparative solution. The amount of the sum of oxidoric acids in the "Safroboz" collection was determined by the spectrophotometric method relative to chlorogenic acid. From the "Safroboz" collection, a clear sample was taken on analytical scales using 50% ethyl alcohol and filtered (Solution A). A certain amount of the solution was diluted with 50% ethyl alcohol in a volumetric flask. 0,00700 гIn parallel, the optical density of the working standard sample (WAS) of chlorogenic acid was determined. To prepare an ICH, the chlorogenic acid was placed in a 50 ml volumetric volumetric flask and dissolved in 50% ethyl alcohol. 0.5 ml of the resulting solution was transferred to a 25 ml volumetric volumetric flask and brought to the mark of the volumetric flask with 50% ethyl alcohol. The optical density of the prepared solution was measured similarly to the tested solutions. 50% ethyl alcohol is used as the compared solution. The amount of the sum of oxidoric acids relative to chlorogenic acid was calculated as a percentage according to the following formula: here D1 - optical density of alcohol extract obtained from phytotea based on the "Safroboz" collection; D0 - OPTIC DENSITY OF CHLORIC ACID; m1raw materials mass, g; m0 - mass of the standard sample, g; C - amount of chlorogenic acid in the standard sample, % (not less than 97%); W - mass loss of the raw material sample during drying, %. The amount of the sum of oxidoric acids relative to chlorogenic acid was 2.22%. Conclusions Similar indicators obtained when determining the total amount of oxidoric acids in the composition of the "Safroboz" collection showed the repeatability of the method. The average 419 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz relative error of the applied method was 1.16%. The obtained results were within the permissible range and testified to the accuracy of the method.