Verification of the Effectiveness of Nano Liquid Fertilizer for Bread Wheat (Triticum Aestivum L.) Production in Bale Highland, Oromia, Ethiopia

@ 2025 | PUBLISHED BY GJR PUBLICATION, INDIA 80 Global Journal of Research in Agriculture & Life Sciences ISSN: 2583-4576 (Online) Volume 05 | Issue 05 | Sept.-Oct. | 2025 Journal homepage: https://gjrpublication.com/gjrals/ Research Article Verification of the Effectiveness of Nano Liquid Fertilizer for Bread Wheat (Triticum Aestivum L.) Production in Bale Highland, Oromia, Ethiopia *Mulugeta Eshetu 1, Regassa Gosa 1, Tesfaye Ketema 1, Tamene Mideksa 2 1Sinana Agricultural Research Center, Soil Fertility Improvement Team, Bale Oromia, Ethiopia. 2Center Director, Sinana Agricultural Research Center, Bale Oromia, Ethiopia. *Corresponding author: Mulugeta Eshetu Sinana Agricultural Research Center, Soil Fertility Improvement Team, Bale Oromia, Ethiopia. Introduction Fertilizers have an axial role in enhancing the food production in developing countries especially after the introduction of high yielding and fertilizer responsive crop varieties (Swati et al., 2023). The soil application of nitrogen and phosphorus fertilizers is not much accepted by scientists, but foliar spray is effective in increasing growth of crops throughout their life period. The unbalanced and indiscriminate use of these chemical fertilizers had a negative impact on soil health, human health and factor production (Spiegal et al., 2020; Jatav et al., 2022 and Poudel et al., 2023). Fertilization management is currently one of the most challenging tasks under the field management. Abstract Excessive synthetic fertilizer use harms soil, water, air, and nutrient efficiency, increasing costs. Nano-fertilizers are advanced synthetic fertilizers with readily available nutrients at the nanoscale, offering higher efficiency and environmental benefits over conventional fertilizers. Despite the fact that no research has been conducted on the effectiveness and potential of nano-fertilizers as alternative fertilizers for bread wheat production in Oromia, Ethiopia. A field experiment was conducted in Sinana and Goba Districts of Bale Highland during the 2024 main cropping season, locally named the bona season (July to December), with the objective of verifying the effectiveness/efficacy of nano fertilizer for bread wheat production in Oromia, Ethiopia. The experiment consisted of three treatments, viz., nano liquid (Nito and Ammo) fertilizer, control (without granular and nano fertilizers), and granular fertilizer. The foliar spray of nano liquid fertilizer was carried out on the 15th, 35th, and 50th days of planting using the factory recommendation. The results showed a significant difference (p < 0.05) in yield and yield components of bread wheat between treatments. The highest grain yield (5800 kgha⁻¹) was obtained from conventional (granular) fertilizer, followed by nano fertilizer (4712 kg ha⁻¹), while the lowest (2419 kg ha⁻¹) was recorded in the control. Partial budget analysis showed that conventional fertilizer resulted in the highest net benefit of 249,450 ETB with a favorable marginal rate of return of 326.31%, while nano fertilizer achieved a net benefit of 211,974.73 ETB with an exceptionally high marginal rate of return of 157,989.47%. It can be concluded that conventional (granular) fertilizer and nano-fertilizer significantly improved bread wheat yield compared to controls, with conventional fertilizer achieving the highest yield. Further research should be recommended on the optimum rate, application times, and viability for bread wheat production across different agro-ecological zones and evaluate the practicality of nano-fertilizers as a supplement or alternative to conventional fertilizers to enhance efficiency and sustainability. Keywords: Nano fertilizer, Nito, Ammo, foliar spray, conventional fertilizer (DAP and urea), bread wheat. Global J Res Agri Life Sci. 2025; 5(5), 80-89 @ 2025 | PUBLISHED BY GJR PUBLICATION, INDIA 81 Despite the fact that mineral fertilizers are necessary for the growth of plants, their prolonged usage poses environmental and health risks, such as surface and groundwater pollution. Foliar application is a method of nourishing plants by spraying liquid fertilizers directly onto their leaves, promoting enhanced absorption in the above-ground parts of the plant (Marzouk et al., 2019 and Borana et al., 2024). Nitrogen and phosphorus applied by foliar application has greater utilization efficiency than N and P supplied directly to the soil. Nano urea nitrogen and phosphorus nutrition at optimum recommended dose of fertilizer increased root length and root area resulting better uptake of other nutrients and this efficient absorption and utilization of other minerals might have supported growth of crop plant (Borana et al., 2024). Nano-fertilizers regulate the nutrient release depending on the crop requirement, making them more efficient than normal fertilizers (Liu and Lal, 2015 and Poudel et al., 2023). Nano fertilizers are a new generation of synthetic fertilizers that contain readily available nutrients in the Nano scale. Nano fertilizers are highly preferred due to their efficiency and environmental friendliness compared to conventional chemical fertilizers (Janmohammadi et al., 2016 and Al-Jubouri et al., 2022). Thus, nano fertilizers were produced as a potential solution to reduce fertilizer loss in the environment and increase fertilizer use efficiency for crop production, hence decreasing the recommended dose of traditional fertilizers. Nanotechnology-based fertilizers can be more soluble or more reactive than their conventional counterparts (Rameshaiah and Jpallavi, 2015; Mahmoud et al., 2017; Al-Jubouri et al., 2022 and Swati et al., 2023). Liquid nano fertilizer which is currently the best alternative to common urea fertilizer. One nano urea liquid particle is 30 nano meters in diameter, with 10,000 time’s higher surface area to volume size than normal granular urea chlorophyll (Rawate et al., 2022). Nano fertilizers have emerged as a promising alternative for ensuring high crop yield while remaining environmentally friendly chlorophyll. Foliar application of nano urea liquid at critical crop growth stages of a plant effectively fulfils its nitrogen requirement and leads to higher crop productivity. The newly created nano fertilizer will reduce chemical fertilizer consumption 80-100 times, saving significant foreign exchange on fertilizer imports (Poudel et al., 2023). This foliar application, is a viable strategy for increasing the availability of nutrients to crops in order to boost output. Adoption of nano fertilizers for revolutionary transformation through nanotechnology, which helps to sustain soil health at the same time ensuring high productivity (Shang et al., 2019 and Borana et al., 2024). Nano fertilizers are recognized for their precise nutrient release and targeted delivery at the nano scale, enhancing plant productivity and mitigating environmental pollution. Nano fertilizers are required in small quantity which reduces toxic effect caused by the extreme use of conventional fertilizers and have higher use efficiency than conventional fertilizers which reduces losses of nutrients. Nano fertilizers are efficiently absorbed by plant because of its ultra-high absorption feature and high surface to volume ratio (Elemike et al., 2019 and Borana et al., 2024). The nano fertilizers are better than conventional fertilizers as they are ecofriendly, cost effective, maintain soil fertility and plant health. Nanotechnology may facilitate enhanced crop yield in an environmentally sustainable and economically steady manner (Ghidan and Antary 2018, and Eid, et al., 2023). Even though nano fertilizers have such crop production, economic and environmental benefit yet not evaluated on the effectiveness/efficacy and adopted to our environmental conditions. Therefore, this studied designed with the objective: to verify the effectiveness/efficacy of Nano fertilizer for bread wheat production and to introduce nano fertilizers as alternative sources of fertilizer for bread wheat production in the region. Material and Methods Description of the study area The study was conducted in Bale districts of Oromia regional state of Ethiopia. The district are located between 6° 31’ 30” N to 7° 25’ 40’’ N and 39° 41’ 40’’ E to 40° 25’ 40’’ E in the southern parts of Oromia Regional State of Ethiopia. The elevation of the area ranges from 1500 to 3500 meter above sea level. The study was conducted in two districts of Bale highland (Goba, and Sinana). Global J Res Agri Life Sci. 2025; 5(5), 80-89 @ 2025 | PUBLISHED BY GJR PUBLICATION, INDIA 82 Figure 1. Map of the study area Climatic condition The Bale climate condition experience eight (8) months of bimodal rainfall that include a heavy rain (July-October) and a small rain (April-June). Some 600-1000 mm falls annually in the lower altitude areas, and 1000-1400 mm in the higher altitude areas. The wet seasons are comparatively warm and the dry seasons are comparably colder at night and warmer during the day. The lowest recorded temperature on the highest is -15ºC and the maximum record was 26ºC. The two cropping seasons in Bale Highlands are called the Ganna (March to June) and Bona (July to December) seasons in which the experiment conducted during this bona season. Global J Res Agri Life Sci. 2025; 5(5), 80-89 @ 2025 | PUBLISHED BY GJR PUBLICATION, INDIA 83 Figure 2. Monthly rainfall, maximum, and minimum temperature and relative humidity of Sinana District Figure 3. Sun shine and evapotranspiration of Sinana District 0 5 10 15 20 25 0 50 100 150 200 250 Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Prc (mm) RH (%) Temp min (℃) Temp max (℃) 0 20 40 60 80 100 120 140 160 180 0 5,000,000 10,000,000 15,000,000 20,000,000 25,000,000 30,000,000 Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Sun shine (J m⁻² day⁻¹) Sun shine (J m⁻² day⁻¹) Eto (mm/m) Global J Res Agri Life Sci. 2025; 5(5), 80-89 @ 2025 | PUBLISHED BY GJR PUBLICATION, INDIA 84 Figure 2. Monthly rainfall, maximum, and minimum temperature and relative humidity of Goba District Figure 3. Sun shine and evapotranspiration of Goba District Experimental design and Treatments The experiment was conducted on farmers’ fields during the main cropping season (July-December) at four (4) location in Sinana and Goba District using farmer’s replications. The land preparation was conducted using both tractor and oxen plow in accordance with conventional tillage frequency. It conducted on the plot size was 5 m x 5 m (25 m2) using 1 m the spacing between blocks and plots with 20 cm between row and plants. Improved bread wheat boku variety was used as test crop at 150 kgha-1 seeds rate. The treatments consist of factory recommended nano fertilizer (1Lha-1 AMMO + 0.5 Lha-1 NITO), Control (without nano and recommended NP) and Recommended NP (69 N kg ha-1 and 69 P2O5 kg ha-1). 0 5 10 15 20 25 0 50 100 150 200 250 300 350 Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Rainfall (mm) Prc. (mm) RH (%) Temp Min (℃) Temp. Max (℃) 0 20 40 60 80 100 120 140 160 0 5,000,000 10,000,000 15,000,000 20,000,000 25,000,000 30,000,000 Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Sun shine (J m⁻² day⁻¹) Sun shine (J m⁻² day⁻¹) Eto (mm) Global J Res Agri Life Sci. 2025; 5(5), 80-89 @ 2025 | PUBLISHED BY GJR PUBLICATION, INDIA 85 Treatments T1 = Factory Recommended Nano fertilizer (1Lha-1 AMMO + 0.5 Lha-1 NITO) T2 = Control (Negative control) T3 = Recommended NP (Glandular form) Methods of Applications Depending on the crop and its nutrient or fertilizer requires, the number of nano fertilizer sprays at 15, 35 and 50 days after germination. Before the spray the nano urea (NITO) bottle was mixed thoroughly and apply in the morning or evening to avoid dew (Table 1). In general, the following table indicated the summarizes the sources, types, times, and doses of nano fertilizer: Table 1. Fertilizers recommendation dose for bread wheat production After Germination Product Fertilizers Requirement per Water Requirement per Hectare (ha) Plot (25 m2) Hectare (ha) Plot (25 m2) 15 days AMMO 123.76 ml 0.3094 ml 123.76 L 309.4 ml NITO 61.88 ml 0.16 ml 35 days AMMO 371.28 ml 0.93 ml 371.28 L 930 ml NITO 371.28 ml 0.93 ml 50 days AMMO 495.05 ml 1.24 ml 495.05 L 1240 ml Total 990.09 ml 2.48 ml 990.09 L 2479.4 ml 433.16 ml 1.09 ml Source of Rate: AGRILABH BEEJ LTD Data collection Plant height, number of productive tillers, spike length, and seed per spike were taken from five (5) randomly selected from the net plot at harvest and were averaged to per plant basis. Aboveground dry biomass yield was recorded from the net plot area harvested and weighted then converted into kgha−1. Thousand kernels weight: It was determined by weighing 1000 kernels sampled from the net plot using a seed counter and sensitive balance. Grain yields was taken by harvesting and threshing the grain yield from total plot area and converted hector basis. Harvest index was calculated as the ratio of grain yield to the above ground dry biomass yield expressed as a percentage. 𝐇𝐚𝐫𝐯𝐞𝐬𝐭 𝐢𝐧𝐝𝐞𝐱 = (𝐄𝐜𝐨𝐧𝐨𝐦𝐢𝐜 𝐲𝐢𝐞𝐥𝐝 )∗𝟏𝟎𝟎 𝐀𝐛𝐨𝐯𝐞 𝐠𝐫𝐨𝐮𝐧𝐝 𝐛𝐢𝐨𝐦𝐚𝐬𝐬 𝐲𝐢𝐞𝐥𝐝 ………………………………… (1) The yield advantage of the fertilized plot compared to the control plot was calculated the following formula: 𝐘𝐢𝐞𝐥𝐝 𝐀𝐝𝐯𝐚𝐧𝐭𝐚𝐠𝐞 = (𝐘𝐢𝐞𝐥𝐝 𝐟𝐫𝐨𝐦 𝐅𝐞𝐫𝐭𝐢𝐥𝐢𝐳𝐞𝐝 𝐏𝐥𝐨𝐭 − 𝐘𝐢𝐞𝐥𝐝 𝐟𝐫𝐨𝐦 𝐂𝐨𝐧𝐭𝐫𝐨𝐥 𝐏𝐥𝐨𝐭)∗𝟏𝟎𝟎 𝐘𝐢𝐞𝐥𝐝 𝐟𝐫𝐨𝐦 𝐂𝐨𝐧𝐭𝐫𝐨𝐥 𝐏𝐥𝐨𝐭 ……… (2) Data Analysis The collected agronomic data were subjected to the procedure of analysis of variance (ANOVA) using GenStat 2015 software program. The comparisons among treatment means were employed by using of Least Significance Difference (LSD) fisher protected at 5% significant level. Partial budget Analysis The partial budget analysis was conducted using a partial budget procedure described by CIMMYT (1988). The price of grain yield of bread wheat, urea, and DAP fertilizers were valued during the study year (2025) at an average open market price at local towns, which were 50 ETB kg−1, 38 ETB kg−1, and 39 ETB kg−1 at Sinana and Goba Districts of Bale highland, respectively. The nano fertilizer prices landing at Addis Abeba which is NITO 18 USD (2,286 ETB) and AMMO 33 USD (4,191 ETB) were considered. Global J Res Agri Life Sci. 2025; 5(5), 80-89 @ 2025 | PUBLISHED BY GJR PUBLICATION, INDIA 86 Result and Discussion Responses of bread wheat production to different fertilizer sources Analysis of variance (ANOVA) revealed that plant height, number of productive tiller, spike length, seed per spike, biomass yield, TKW while not significant (P<0.05) difference among harvested index between the treatments. Plant height The analysis of variance showed a significant effect (p ≤ 0.05) of treatments on plant height (Table 2). The highest plant height (89.50 cm) was recorded with conventional granular fertilizer, followed by nano fertilizer (85.55 cm), while the lowest (72.90 cm) was in the control. This might be due to optimum nitrogen and phosphorus physiological process of plant as it promotes vegetative growth. The similar results were also found by (Choudhary et al (2018) and Borana et al (2024) who reported that nitrogen and phosphors promoting cell division to development, vigorous root system and higher yield. The highest plant height with nano fertilizer may be due to nutrient uptake during foliar application. These findings align with Al-Juthery et al (2018), Rawate et al (2022), Maheta et al (2023), and Swati et al (2023). The synergy between conventional and nano fertilizers enhances nutrient absorption at the cellular level, optimizing growth and metabolic functions like photosynthesis, ultimately increasing plant height (Borana et al., 2024). Number of tiller The analysis of variance indicated a significant effect (p ≤ 0.05) of treatments on the number of productive tillers (Table 2). The highest number of productive tillers (4) was observed with conventional granular fertilizer, followed by nano fertilizer (3.30), while the control recorded the lowest (2) (Table 2). The synergy between conventional and nano fertilizers boosts nutrient absorption, enhancing growth and photosynthesis (Borana et al., 2024). This increases photosynthate translocation, leading to more reproductive tillers. Spike length The ANOVA results revealed a significant effect (p ≤ 0.05) of treatments on bread wheat spike length (Table 2). The longest spike (8.16 cm) was recorded with conventional granular fertilizer, followed by nano fertilizer (7.285 cm), while the control had the shortest (4.98 cm) spike length (Table 2). Increased nutrient availability enhances photosynthate translocation, accelerating sink development and resulting in longer spike length (Borana et al., 2024). Number of seed per spike The results of the ANOVA showed that the treatments had a significant (p ≤ 0.05) effect on the number of seeds per spike (Table 2). Conventional granular fertilizer produced the highest number of seed per spike (52.95) followed by nano fertilizer (45.75), whereas the control produced lowest number of seeds per spike (35.30) (Table 2). Nano fertilizers enhance enzymatic reactions and hormone regulation, reducing ovarian abortion and improving pollination, leading to more seed per spike (Borana et al., 2024). Biomass yield The results of the ANOVA showed that the treatments had a significant (p ≤ 0.05) effect on the biomass yield (Table 2). The highest biomass yield (18640 kgha-1) was recorded with conventional granular fertilizer, followed by nano fertilizer (13210 kgha-1), while the lowest (7700 kgha-1) was in the control. The highest biomass yield in nano fertilizer might be due to the foliar application might be due to quick absorption by plant and easiness of translocation, which aided in better rates of photosynthesis and more dry matter accumulation, that give higher biomass yield. The similar results were also found by Chandana et al (2021); Rawate et al (2022); Maheta et al (2023) and Swati et al (2023). Nano fertilizer improve nutrient release resulting in a higher photosynthetic rate and ultimately, a higher biomass yield accumulation (Adhikari et al., 2014 and Poudel et al., 2023). Nano fertilizers enhance phosphorus absorption and slow-release nano DAP boosts chlorophyll synthesis, increasing biomass yield. Studies confirm high biomass like conventional fertilizers (Liu and Lal, 2014; Borana et al., 2024). Nano fertilizers significantly enhance grain yield by improving plant height, spike length, grains per spike, and 1000-grain weight (Ahmadian et al., 2021). This leads to a substantial increase in overall biological yield. Grain yield The results of the ANOVA showed that the treatments had a significant (p ≤ 0.05) effect on the grain yield (Table 2). The highest grain yield (5800 kgha-1) was recorded with conventional granular fertilizer, followed by nano fertilizer (4712 kgha-1), while the lowest (2419 kgha-1) was in the control. This might be due to nano fertilizer improves nutrient absorption, resulting in optimal growth of plant parts and improved metabolic processes such as photosynthesis, which results in higher accumulation of photosynthetic and translocation to the plant’s economic parts, improving crop growth, development and yield. A similar result was seen on broad bean, where the use of nano-fertilizer provided better nutrient Global J Res Agri Life Sci. 2025; 5(5), 80-89 @ 2025 | PUBLISHED BY GJR PUBLICATION, INDIA 87 accumulation and increased growth activity due to smart delivery system of the fertilizers (Benzon et al., 2015; El-Azizy et al., 2021 and Poudel et al., 2023). The observed yield advantage demonstrates a 139.77% increase with conventional granular fertilizer and a 94.79% increase with nano fertilizer compared to the control. This indicates that conventional granular fertilizers supply nutrients more rapidly and in greater quantities, resulting in a more substantial yield increase. In contrast, nano fertilizers, despite their higher nutrient use efficiency, release nutrients gradually, leading to a lower but still significant yield improvement. The superior yield response to conventional fertilizers suggests that crops benefit from the immediate availability of essential macronutrients, whereas the slower nutrient release from nano fertilizers contributes to steady but comparatively lower yield gains. These findings align with previous studies (Aziz et al., 2016; Borana et al., 2024), highlighting the influence of agronomic practices. Foliar nutrient sprays with nano DAP and optimal fertilization significantly enhance yield (Sahu et al., 2022; Borana et al., 2024). Nano fertilizers significantly enhance grain yield by improving plant height, spike length, grains per spike, and 1000grain weight (Ahmadian et al., 2021). This leads to a substantial increase in overall biological yield. These findings closely align with results reported by Attri et al (2022), Borana et al (2024), and others, confirming similar trends in yield improvements. Table 2. Responses of bread wheat yield and yield components to nano liquid fertilizer foliar application and granular NP fertilizers Treatment PH (cm) NT SL (cm) SPS BM (kgha-1) Gy (kgha-1) HI (%) TKW (g) LNF 85.55a 3.30b 7.285a 45.75b 13210b 4712b 36.82 42.02b Control 72.90b 2.00c 4.980b 35.30c 7700c 2419c 32.83 34.39c RNP 89.50a 4.00a 8.160a 52.95a 18640a 5800a 31.37 47.62a Mean 82.6 3.1 6.81 44.67 13183. 4310. 33.67 41.34 LSD (0.05) 6.85 0.5028 0.907 5.066 3697.1 673.9 NS 4.453 CV (%) 4.8 9.4 7.7 6.6 16.2 9 18.8 6.2 Where, LNF = Liquid nano fertilizer, RNP = recommended nitrogen and phosphors, PH = plant height, NT = number of productive tiller, SL = spike Length, SPS = seed per spike, BM = biomass yield, GY = grain yield, HI = Harvesting index, TKW = thousand kernel weight, NS = non-significant among the treatment Partial Budget Analysis Partial budget analysis showed that conventional fertilizer resulted in the highest net benefit of 249, 450 ETB with a favorable marginal rate of return of 326.31 %, while nano fertilizer achieved a net benefit of 211, 974.73 ETB with an exceptionally high marginal rate of return of 157989.47%. Table 3. Partial Budget Analysis Treatments N P2O5 AMMO NITO AdGY (kg ha-1) TB TVC NB MRR (%) kg ha-1 (L/ha) ETB ha-1 Control 0 0 0 0 2177.1 108855 0 108855 LNF 0 0 1 0.5 4240.8 212040 65.27 211974.73 157989.47 RNP 69 69 0 0 5220 261000 11550 249450 326.31 Where, LNF = Liquid nano fertilizer, RNP = recommended nitrogen and phosphors, AdGY = adjusted grain yield, TB = total benefit, TVC = total variable cost, NB = total net benefit, marginal net return Conclusion The verification study evaluating the effectiveness of liquid nano fertilizer against a control (negative control) and conventional granular fertilizer demonstrated a significant influence on bread wheat yield and its components. The highest grain yield (5800 kg ha⁻¹) was obtained from conventional (granular) fertilizer, followed by nano fertilizer (4712 kg ha⁻¹), while the lowest (2419 kg ha⁻¹) was recorded in the control. Partial budget analysis showed that conventional fertilizer resulted in the highest net benefit of 249, 450 ETB with a favorable marginal rate of return of 326.31%, while nano fertilizer achieved a net benefit of 211,974.73 ETB with an exceptionally high marginal rate of return of Global J Res Agri Life Sci. 2025; 5(5), 80-89 @ 2025 | PUBLISHED BY GJR PUBLICATION, INDIA 88 157,989.47%. These findings confirm that both conventional and nano fertilizers enhanced wheat yield compared to the control, with conventional fertilizer demonstrating the greatest yield advantage. Consequently, it can be understood that, in light of the research that has been conducted, nano fertilizer deserves to be officially registered by the agricultural authority as input for wheat production and additional for further studied. Recommendations On-farm trials across diverse agro-ecological zones are necessary to validate the consistency of the results, the nano fertilizer as a supplement, and its equivalency with conventional fertilizer recommended. Given the promising results observed with nano liquid fertilizer, it is recommended that the product be registered, and further studies be conducted across diverse agro-ecological zones to validate its effectiveness and adaptability under varying environmental and soil conditions. Further research should focus on determining the optimal rates, timing, and method of application to maximize bread wheat yield and minimize soil nutrient losses. References 1. Adhikari, T., Kundu, S., Meena, V., & Rao, A. S. (2014). Utilization of nano rock phosphate by maize (Zea mays L.) crop in a vertisol of Central India. Journal of Agricultural Science and Technology, 4, 384–394. 2. Al-Jubouri, M. A. N., Abdlrhmaam, A. J., & Al-Ardeeni, A. A. (2022). 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