Influence of Surface Modification on Iron Oxide Peroxidase-Mimic Activity for Antibacterial Application
Simnani, Faizan Zarreen; Ramanathan, Rajesh; Bansal, Vipul; PARIS, Jérémy; Chevolot, Yann; Monnier, Virginie
- Publisher
- Zenodo
- Language
- en
Abstract
Concerns over microbial drug resistance drive extensive research for effective, stable, and broadspectrum antimicrobial agents. Iron oxide nanoparticles (IONPs) have emerged as promising candidates owing to their intrinsic catalytic, magnetic properties, and biocompatibility [1]. These IONPs exhibit peroxidase (POD)-like activity, enabling the generation of reactive oxygen species (ROS) with potent antibacterial effects [2]. However, immediate radical quenching due to ultra-short halftime hampers diffusion and bactericidal efficacy. [3]. Our study aimed to investigate the POD-like activity of diverse IONP formulations and evaluate the impact of surface modification on their catalytic activity and antibacterial efficiency. Commercial NPs employed in our investigation included superparamagnetic iron oxide NPs (SPIONs), palladium-coated SPIONs (SPION-Pd), and polymercoated γ-Fe2O3 NPs. Additionally, we have synthesized Fe3O4 NPs coated with oleic acid (OA-Fe3O4). Characterization of these IONPs was conducted using TEM, EDS, FTIR, and DLS techniques. Evaluation of POD-like activity and leached ions employed the TMB-H2O2 and ABTS-H2O2 assays, with absorbance measurements conducted at 650 nm and 405 nm, respectively. Notably, aptamer adsorption onto NPs induced significant alterations in the zeta potential of IONPs, corroborated by the presence of a phosphate peak in the FTIR spectra. Our preliminary findings unveiled distinctive responses to aptamer adsorption across various IONP formulations. Substrate-specific catalytic activity was observed, with OA-Fe3O4 and SPIONs exhibiting a notable increase in POD-like activity in the TMB-H2O2 assay upon aptamer adsorption. Conversely, in SPION-Pd and polymer-coated γ-Fe2O3, catalytic activity diminished in the TMB reaction following aptamer adsorption. In the ABTS-H2O2 assay, a decrease in POD-like activity was observed in OA-Fe3O4 and SPIONs upon aptamer adsorption. Our preliminary data indicate successful aptamer functionalization, and enhanced POD-like activity post-adsorption, suggesting potential for improved antibacterial efficacy. Future endeavors will focus on the identification of specific aptamers, antibodies, and nanobodies targeting pathogenic bacteria, alongside investigations into the capture and antibacterial activity of these nanoparticle formulations. 1. Asma Ghazzy, et al., Magnetic iron oxide-based nanozymes: from synthesis to application. Nanoscale Advances, 2024/03/12. 6(6).2. Sang, Y., et al., Construction of Nanozyme‐Hydrogel for Enhanced Capture and Elimination of Bacteria.Advanced Functional Materials, 2019/05/01. 29(22).3. Gao, L., et al., Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nature Nanotechnology 2007 2:9, 2007-08-26. 2(9).
Full text
HAL Id: hal-04725595 https://hal.science/hal-04725595v1 Submitted on 10 Oct 2024 HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Influence of Surface Modification on Iron Oxide Peroxidase-Mimic Activity for Antibacterial Application Faizan Zarreen Simnani, Rajesh Ramanathan, Vipul Bansal, Jérémy Paris, Chloé Gervasoni, Yann Chevolot, Virginie Monnier To cite this version: Faizan Zarreen Simnani, Rajesh Ramanathan, Vipul Bansal, Jérémy Paris, Chloé Gervasoni, et al.. Influence of Surface Modification on Iron Oxide Peroxidase-Mimic Activity for Antibacterial Application. 7th Plenary Days of GDR-B2i (Bioingénierie des interfaces), Jul 2024, Mulhouse, France. �hal-04725595�
Influence of Surface Modification on Iron Oxide PeroxidaseMimic Activity for Antibacterial Application Faizan Zarreen Simnani1,2, Rajesh Ramanathan2, Vipul Bansal2, Jérémy Paris3, Chloé Gervasoni3, Yann Chevolot1, Virginie Monnier1,* 1 Institut des Nanotechnologies de Lyon, CNRS, Ecole Centrale de Lyon, INSA Lyon, Universite Claude Bernard Lyon 1, CPE Lyon, INL, UMR5270, Ecully, France 2 Sir Ian Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory, RMIT University, Melbourne, Australia 3 SON Company, Dijon, France (www.sonsas.com) Introduction Methods Preliminary Results Conclusions ➢Nanoparticles are well distributed and spherically shaped with few irregularities. ➢Our preliminary data indicates successful functionalization of aptamer on the surface of nanoparticles. ➢Nanoparticles in the presence of aptamer, change their peroxidase mimicking behavior, enhanced in TMB substrate which shows potential for antibacterial activity. ➢All the nanozymes are substrate-dependent and electrostatic interaction between the nanoparticle and substrate defines the catalytic activity. ➢Leaching of iron ions is observed which influences the catalytic activity of nanoparticles. Acknowledgement Figure 1: Schematic of hydroxyl radical release from nanoparticle and depiction of its efficiency in aptamer-assisted and unassisted iron oxide nanoparticle for antibacterial activity. References ➢A. Ghazzy et al., Nanoscale Advances, vol. 6, no. 6, pp. 1611–1642, Jan. 2024. ➢Y. Sang et al., Advanced Functional Materials, vol. 29, no. 22, Apr. 2019. ➢L. Gao et al., Nature Nanotechnology, vol. 2, no. 9, pp. 577–583, Aug. 2007. ➢H. Dong et al., Nature Communications, vol. 13, no. 1, Sep. 2022. 𝐹𝑒2+ + 𝐻2𝑂2 → 𝐹𝑒3+ + ∙ 𝑂𝐻 + 𝑂𝐻− 𝐹𝑒3+ + 𝐻2𝑂2 → 𝐹𝑒2+ + ∙ 𝑂𝑂𝐻 + 𝐻+ (i) (ii) 𝐻2𝑂2 + 𝑇𝑀𝐵 𝐼𝑟𝑜𝑛 𝑂𝑥𝑖𝑑𝑒 𝑁𝑃 2 𝐻2 O + oxidized TMB 𝐻2𝑂2 + 𝐴𝐵𝑇𝑆 𝐼𝑟𝑜𝑛 𝑜𝑥𝑖𝑑𝑒 𝑁𝑃 2 𝐻2 O + oxidized ABTS Equations: (i) Catalytic cycle where Fe²⁺ is oxidized to Fe³⁺, producing hydroxyl radicals, and Fe³⁺ is reduced back to Fe²⁺ by H₂O₂. (ii) TMB (tetramethylbenzidine) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) is oxidized by hydroxyl radicals to form TMB and ABTS radical cations respectively Figure 3: TEM image: (i) SPION, (ii) OA-Fe3O4, (iii) SPIO-Pt, (iv) SPIO-Pt higher magnification, (v) SPIOPd, (vi) SPIO-Au. Scale bar: (i-iii, v, vi): 50nm; (vi): 10nm Figure 5: FT-IR spectra of iron oxide and the hybrid nanoparticles showing Iron oxide characteristic peaks. Figure 4: Atomic percent of iron and noble metals in the hybrid nanoparticles. Figure 6: Change in zeta potential after aptamer (Apt) adsorption Future Perspectives ➢Determine the substrate specificity by applying the Michaelis-Menten model. ➢Further investigate the aptamer which is highly specific and selective toward the selective bacteria. ➢Capture and antibacterial efficiency of the aptamer functionalized nanoparticles. ➢Investigate the Fenton reaction that may occur due to the leached ions in the reaction mixture. Figure 7: Influence in Peroxidase activity of iron oxide nanoparticle after aptamer (Apt) surface medication. Figure 8: Influence in Peroxidase activity of hybrid NPs after aptamer surface medication. Figure 9: Catalytic activity of leaked ions in percentage relative to the nanoparticles in two substrates, TMB (left) and ABTS (right). iii iii iv vvi 1. 2. 3. 4. 6. 7. 8. 9. Co-funded by the European Union under the Marie Skłodowska-Curie Grant Agreement No 101081465 (AUFRANDE). ➢Urgent need to address antibiotic resistance in microorganisms. ➢Extensive research on developing highly effective, stable, and broad-spectrum antimicrobial treatments. ➢Iron oxide nanoparticles (IONPs) are promising due to: Intrinsic catalytic, magnetic properties and biocompatibility. ➢Iron oxide nanoparticles exhibit peroxidase-like activity (POD), producing reactive oxygen species (ROS) with strong antibacterial properties. ➢Rapid quenching of radicals due to ultrashort half-life hinders diffusion and bactericidal activity. ➢Study aims: i) Examine POD-like activity of various IONP formulations, ii) Assess the influence of surface modification on catalytic activity and antibacterial effectiveness. Figure 2: Method (i →vi) of aptamer adsorption and investigation of catalytic behavior of aptamer functionalized IONPs. Colored product (Prominent) Colored product (Not prominent) Surface Available Surface Not Available Expected catalytic behavior POD inhibits (i) (ii) (iii) (iv) (v) (vi) (650nm) (405nm) 5.
