Scanning electron microscope images of Chlorella sorokiniana culture 20 days old
Abstract
Scanning electron microscope images of Chlorella sorokiniana culture 20 days old without fixation in low vacuum are presented. Each image is supplemented by description of the preparation of the sample and the data on the imaging technique and equipment. The data are curated by Anna Romolo and Veronika Kralj-Iglič, University of Ljubljana, Faculty of Health Sciences, Laboratory of Clinical Biophysics, Ljubljana, Slovenia.
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Figure 1 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 2 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 3 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 4 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 5 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 6 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 7 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 8 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 9 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 16 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 17 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 18 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 19 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 20 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 21 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 22 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 23 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 24 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.
Figure 25 - SEM of Chlorella sorokiniana culture Cultivation of the algae strain CCAP 211/8K was from the culture Collection of Algae and Protozoa (CCAP) (Oban, Scotland) was grown in 50 mL aliquots of 3N-BBM+V homemade medium (https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V.pdf) in 100 ml Erlenmeier flasks placed on the orbital shaker (120 rpm) in a growth cabinet with the following conditions: temperature 22C and a continuous photo flux oF20 M/m2/s (Phillips MST TL-D Reflex 36W840 1 SLV/25 lamps, Amsterdam, Netherlands). The medium was inoculated with approximately 0.5 10 6cells/mL. The culture was taken at early stationary phase (day 20). Scanning Electron Microscopy (SEM) Samples were loaded onto aluminum stands covered with thin layer of polymers based on cyanoacrylates (Jampani et al., 2024) and examined with a Thermo Fisher Scientific Apreo 2 microscope with EDS/EBSD detector, LOW vacuum mode.