Supplementary Materials - Community Challenge towards Consensus on Characterization of Biological Tissue:C4Bio's first findings

Community Challenge towards Consensus on Characterization of Biological Tissue: First Findings Supplemental Figures Preprint submitted to Journal of Biomechanics November 22, 2024 1. Standard Deviation on Stress/Strain Figure S1: Engineering stress - engineering strain curves of longitudinal specimen types obtained in round 1, grouped per research group (RG). Vertical lines represent the standard deviation (SD) of stress values at strain levels of 0.1, 0.2, 0.3 and 0.4. Horizontal lines indicate the SD of strain values at the mean stress value corresponding to these strain levels. Measurement uncertainty on the mean stress value is shown at each strain level, accounting for a 0.1 N load cell uncertainty and 0.02 mm uncertainty in specimen thickness and width measurements. 2 Figure S2: Engineering stress - engineering strain curves of longitudinal specimen types obtained in round 2, grouped per research group (RG). Vertical lines represent the standard deviation (SD) of stress values at strain levels of 0.1, 0.2, 0.3 and 0.4. Horizontal lines indicate the SD of strain values at the mean stress value corresponding to these strain levels. Measurement uncertainty on the mean stress value is shown at each strain level, accounting for a 0.1 N load cell uncertainty and 0.02 mm uncertainty in specimen thickness and width measurements. 3 Figure S3: Engineering stress - engineering strain curves of circumferential specimen types obtained in round 1, grouped per research group (RG). Vertical lines represent the standard deviation (SD) of stress values at strain levels of 0.1, 0.2, 0.3 and 0.4. Horizontal lines indicate the SD of strain values at the mean stress value corresponding to these strain levels. Measurement uncertainty on the mean stress value is shown at each strain level, accounting for a 0.1 N load cell uncertainty and 0.02 mm uncertainty in specimen thickness and width measurements. 4 Figure S4: Engineering stress - engineering strain curves of circumferential specimen types obtained in round 2, grouped per research group (RG). Vertical lines represent the standard deviation (SD) of stress values at strain levels of 0.1, 0.2, 0.3 and 0.4. Horizontal lines indicate the SD of strain values at the mean stress value corresponding to these strain levels. Measurement uncertainty on the mean stress value is shown at each strain level, accounting for a 0.1 N load cell uncertainty and 0.02 mm uncertainty in specimen thickness and width measurements. 5 Figure S5: Engineering stress - engineering strain curves of synthetic 1A specimen types obtained in round 1, grouped per research group (RG). Vertical lines represent the standard deviation (SD) of stress values at strain levels of 0.1, 0.2, 0.3 and 0.4. Horizontal lines indicate the SD of strain values at the mean stress value corresponding to these strain levels. Measurement uncertainty on the mean stress value is shown at each strain level, accounting for a 0.1 N load cell uncertainty and 0.02 mm uncertainty in specimen thickness and width measurements. 6 Figure S6: Engineering stress - engineering strain curves of synethetic 1B specimen types obtained in round 1, grouped per research group (RG). Vertical lines represent the standard deviation (SD) of stress values at strain levels of 0.1, 0.2, 0.3 and 0.4. Horizontal lines indicate the SD of strain values at the mean stress value corresponding to these strain levels. Measurement uncertainty on the mean stress value is shown at each strain level, accounting for a 0.1 N load cell uncertainty and 0.02 mm uncertainty in specimen thickness and width measurements. 7 Figure S7: Engineering stress - engineering strain curves of synethetic 2 specimen types obtained in round 1, grouped per research group (RG). Vertical lines represent the standard deviation (SD) of stress values at strain levels of 0.1, 0.2, 0.3 and 0.4. Horizontal lines indicate the SD of strain values at the mean stress value corresponding to these strain levels. Measurement uncertainty on the mean stress value is shown at each strain level, accounting for a 0.1 N load cell uncertainty and 0.02 mm uncertainty in specimen thickness and width measurements. 8 2. Covariance of Stress/Strain Figure S8: Covariance of engineering stress versus the covariance of engineering strain at strain levels 0.1 to 0.5 with 0.1 steps. Covariance values are calculated per research group. Research groups for which both covariance values fall within the threshold of 0.33 are represented by dots, while those with either covariance value exceeding this threshold are marked with ‘×’ markers. The percentage of research groups whose covariance values remain within the threshold is indicated on each plot. The data are organised by specimen type and separated by testing round 1 (R1) and 2 (R2). 9