1
Molecula and Beha iou al e ec s o social isola ion on Neg 1-de icien mice.
A pana Reinsbe g1, Ka yayani Singh1, Mohan Jaya am1, Kaie Mikheim1, Ma i-Anne Philips1, Ee o Vasa 1.
qPCR esul s ables
qPCR Ta ge s
Sex
P e on al co ex
Two-Way ANOVA
Inhibi o y neu onal
ma ke s
G oup housed
Social isola ion
Fac o s
G oup W
Mean ±SEM
( ans o med da a)
G oup Neg 1-/-
Mean ±SEM
( ans o med da a)
SI W
Mean ±SEM
( ans o med da a)
SI Neg 1-/-
Mean ±SEM
( ans o med da a)
X: In e ac ion
#: T ea men
$: Geno ype
F
( ans o med
da a)
P
( ans o med
da a)
Gad1
Males
-6.135 ± 0.1100
-6.931± 0.08753
-6.314 ± 0.1371
-6571 ± 0.09341
Main e ec : -$$
In e ac ion
T ea men
Geno ype
Pos -hoc: -
1.082
0.2180
10.29
0.3066
0.6439
0.0032
Females
-5.545 ± 0.08811
-5564 ± 0.4744
-5.584 ± 0.03948
-5.615 ± 0.06440
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.009287
0.4979
0.1562
0.9238
0.4852
0.6951
Gad2
Males
-4.035 ± 0.07067
-4.101 ± 0.1117
-4.008 ± 0.09015
-3.893 ± 0.09879
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.9173
1.560
0.06734
0.3454
0.2207
0.7969
Females
-3.582 ± 0.04560
-3.474 ± 0.08224
-3.560 ± 0.05225
-3.387 ± 0.03626
Main e ec : -$
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.3230
0.09224
6.122
0.05733
0.3433
0.0182
P alb
Males
-0.9851 ± 0.08887
-1.006 ± 0.06772
0.9479 ± 0.03572
-1.053 ± 0.06306
Main e ec : -
In e ac ion
T ea men
0.3984
0.005404
0.5324
0.9419
2
Geno ype
Pos -hoc: -
0.8918
0.3521
Females
-1.313 ± 0.07495
-1.374 ± 0.04948
-1.327 ± 0.05317
-1.454 ± 0.04771
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.3412
0.6699
2.693
0.5628
0.4185
0.1095
Gad2 _SI_Unpai ed
- es _Welch’s es
Females
-3.560 ± 0.05225
-3.387 ± 0.03656
T ea men
0.0152
3
qPCR Ta ge s
Sex
Hippocampus
Two-Way ANOVA
Inhibi o y neu onal
ma ke s
G oup housed
Social isola ion
Fac o s
G oup W
Mean ±SEM
( ans o med da a)
G oup Neg 1-/-
Mean ±SEM
( ans o med da a)
SI W
Mean ±SEM
( ans o med da a)
SI Neg 1-/-
Mean ±SEM
( ans o med da a)
X: In e ac ion
#: T ea men
$: Geno ype
F
( ans o med
da a)
P
( ans o med
da a)
Gad1
Males
-4.509 ± 0.1851
-4.828 ± 0.1417
-4.474 ± 0.08811
-4.002 ± 0.1429
Main e ec : -XX##
In e ac ion
T ea men
Geno ype
Pos -hoc: **C l Neg 1-/- s SI Neg 1-/-
7.540
8.920
0.2825
0.0095
0.0051
0.5984
Females
-4.799 ± 0.1587
-4.839 ± 0.1422
-4.631 ± 0.1899
-4.722 ± 0.09087
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.6139
0.05374
0.05374
0.4386
0.8180
0.8180
Gad2
Males
-3.518 ± 0.08565
-3.686 ± 0.1017
-3.565 ± 0.06295
-3.634 ± 0.06796
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.3655
0.001450
2.012
0.5493
0.9698
0.1560
Females
-3.589 ± 0.05239
-3.580 ± 0.05646
-3.492 ± 0.04706
-3.542 ± 0.04489
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.3537
1.763
0.1669
0.5559
0.1928
0.6854
P alb
Males
-2.575 ± 0.07649
-2.809 ± 0.05187
-2.641 ± 0.06595
-3.002 ± 0.08894
Main e ec : -$$$
In e ac ion
T ea men
Geno ype
Pos -hoc: **SI W s SI Neg 1-/-
0.7538
3.096
16.39
0.3915
0.0878
0.0003
Females
-2.606 ± 0.04762
-2.706 ± 0.05406
-2.563 ± 0.09268
-2.809 ± 0.08078
Main e ec : $
In e ac ion
T ea men
Geno ype
Pos -hoc: -
1.053
0.1799
5.878
0.3117
0.6740
0.0205
4
qPCR Ta ge s
Sex
P e on al co ex
Two-Way ANOVA
Neu o ophic- ela ed
Genes
G oup housed
Social isola ion
Fac o s
G oup W
Mean ±SEM
( ans o med da a)
G oup Neg 1-/-
Mean ±SEM
( ans o med da a)
SI W
Mean ±SEM
( ans o med da a)
SI Neg 1-/-
Mean ±SEM
( ans o med da a)
X: In e ac ion
#: T ea men
$: Geno ype
F
( ans o med
da a)
P
( ans o med
da a)
Fg 2
Males
-8.735 ± 0.1374
-8.793 ± 0.1880
-8.661 ± 0.2081
-8.570 ± 0.1743
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.1644
0.6567
0.007995
0.6878
0.4235
0.9293
Females
-7.727 ± 0.1807
-8.018 ± 0.1119
-7.504 ± 0.1359
-7.904 ± 0.07371
Main e ec : -$
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.1707
1.618
6.748
0.6824
0.2128
0.0142
Bdn
Males
-4.180 ± 0.09484
-4.266 ± 0.07222
-4.156 ± 0.05230
-4.-65 ± 0.02280
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
1.942
3.093
0.001690
0.1737
0.0888
0.9675
Females
-4.503 ± 0.08394
-4.449 ± 0.08081
-4.324 ± 0.08372
-4.552 ± 0.1219
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
2.253
0.1601
0.8590
0.4121
0.6914
0.3601
N k2(T kB)
Males
-6.149 ± 0.1134
-6198 ± 0.08375
-6.004 ± 0.1182
-6.207 ± 0.04299
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.5500
0.4321
1.495
0.4637
0.5157
0.2304
Females
-5.514 ± 0.1133
-5.749 ± 0.08784
-5.504 ± 0.08871
-5793 ± 0.08427
Main e ec : -$$
In e ac ion
T ea men
Geno ype
0.07990
0.03186
7.599
0.7791
0.8594
0.0092
5
Pos -hoc: -
Tc 4
Males
-5.674 ± 0.1850
-5.815 ± 0.1477
-5.574 ± 0.1635
-5.658 ± 0.1946
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.02732
0.5524
0.4237
0.8697
0.4623
0.5193
Females
-4.712 ± 0.2134
-5.348 ± 0.07288
-5.008 ± 0.2712
-5.430 ± 0.1239
Main e ec : -$
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.3026
0.9426
7.379
0.5859
0.3385
0.0103
6
qPCR Ta ge s
Sex
Hippocampus
Two-Way ANOVA
Neu o ophic- ela ed
Genes
G oup housed
Social isola ion
Fac o s
G oup W
Mean ±SEM
( ans o med da a)
G oup Neg 1-/-
Mean ±SEM
( ans o med da a)
SI W
Mean ±SEM
( ans o med da a)
SI Neg 1-/-
Mean ±SEM
( ans o med da a)
X: In e ac ion
#: T ea men
$: Geno ype
F
( ans o med
da a)
P
( ans o med
da a)
Fg 2
Males
-6.153 ± 0.1410
-6.381 ± 0.09836
-6.109 ± 0.08745
-5.884 ± 0.06689
Main e ec : -X#
In e ac ion
T ea men
Geno ype
Pos -hoc: *C l Neg 1-/- s SI Neg 1-/-
4.759
6.756
0.0001530
0.0360
0.0136
0.9902
Females
-6.321 ± 0.1505
-6.512 ± 0.2033
-6.218 ± 0.1296
-6.306 ± 0.11156
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.1193
1.047
0.8531
0.7318
0.3132
0.3620
Bdn
Males
-3.537 ± 0.09984
-3.681 ± 0.05723
-4.047 ± 0.08875
-4.042 ± 0.1114
Main e ec : ####
In e ac ion
T ea men
Geno ype
Pos -hoc: **C l W s SI W
0.6795
23.15
0.5947
0.4154
<0.0001
0.4458
Females
-3.999 ± 0.07033
-4.082 ± 0.04816
-4.063 ± 0.04676
-4.135 ± 0.07956
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.008121
0.8225
1.423
0.9287
0.3708
0.2412
N k2(T kB)
Males
-4.368 ± 0.2485
-4.885 ± 0.1305
-4.302 ± 0.09471
-3.775 ± 0.1247
Main e ec : -XX##
In e ac ion
T ea men
Geno ype
Pos -hoc: ***C l Neg 1-/- s SI Neg 1-/-
10.09
12.81
0.0008022
0.0032
0.0011
0.9776
Females
-4.730 ± 0.2250
-4.741 ± 0.2075
-4.531 ± 0.2345
-4.583 ± 0.1211
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.01044
0.7796
0.02479
0.9192
0.3831
0.8758
7
Tc 4
Males
-2.418 ± 0.2028
-2.768 ± 0.2733
-2.714 ± 0.1015
-2.094 ± 0.1248
Main e ec : X
In e ac ion
T ea men
Geno ype
Pos -hoc: -
6.415
0.9797
0.4979
0.0160
0.3291
0.4851
Females
-2.935 ± 0.2549
-2.925 ± 0.2394
-2.722 ±0.2521
-2.719 ± 0.09106
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.0003054
0.9043
0.0008342
0.9862
0.3480
0.9771
Tc 4_Neg 1-/-_Unpai ed
_Welch’s es
Males
0.1671 ± 0.02314
0.2410 ± 0.01907
Geno ype
0.0250
8
qPCR Ta ge s
Sex
P e on al co ex
Two-Way ANOVA
Synap ic adhesion
G oup housed
Social isola ion
Fac o s
G oup W
Mean ±SEM
( ans o med da a)
G oup Neg 1-/-
Mean ±SEM
( ans o med da a)
SI W
Mean ±SEM
( ans o med da a)
SI Neg 1-/-
Mean ±SEM
( ans o med da a)
X: In e ac ion
#: T ea men
$: Geno ype
F
( ans o med
da a)
P
( ans o med
da a)
Cn n1
Males
-3.448 ± 0.09964
-3.679 ± 0.09330
-3.546 ± 0.08475
-3.639 ± 0.1167
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.4959
0.08564
2.700
0.4860
0.7715
0.1093
Females
-2.982 ± 0.06758
-3.117 ± 0.06181
-3.074 ± 0.08346
-3.181 ± 0.1001
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.0311
0.9659
2.309
0.8609
0.3325
0.1373
N m1a
Males
-4.121 ± 0.1063
-4.326 ± 0.1007
-4.178 ± 0.1233
-4.335 ± 0.1671
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.03795
0.06947
2.096
0.08467
0.7937
0.1566
Females
-3.546 ± 0.1123
-3.769 ± 0.09255
-3.556 ± 0.1069
-3.848 ± 0.1135
Main e ec : $
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.1085
0.1767
5.826
0.7438
0.6767
0.0210
N m1b
Males
-6.803 ± 0.09637
-6.756 ± 0.06743
-6.783 ± 0.1102
-6.484 ± 0.1896
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
1.113
1.483
2.081
0.2994
0.2323
0.1588
Females
-5.927 ± 0.08949
-6.108 ± 0.07492
-6.045 ± 0.1170
-6.165 ± 0.09386
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.1929
0.6034
2.659
0.66632
0.4425
0.1119
Lsamp1a
Males
-6.071 ± 0.07266
-6.130 ± 0.1241
-6.303 ± 0.1702
-6.287 ± 0.1334
Main e ec : -
9
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.080
2.154
0.025
0.7789
0.1514
0.8750
Females
-5.384 ± 0.1504
-5.346 ± 0.07430
-5.293 ± 0.04744
-5.296 ± 0.09474
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.042
0.5374
0.033
0.8371
0.4684
0.8560
Lsamp1b
Males
-5.809 ± 0.02372
-6.089 ± 0.09130
-6.103 ± 0.1440
-6.003 ± 0.1399
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
2.646
0.797
0.5956
0.1136
0.3787
0.4459
Females
-5.272 ± 0.08106
-5.338 ± 0.06186
-5.329 ± 0.04886
-5.490 ± 0.1427
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.061
0.347
0.505
0.8060
0.5591
0.4817
Neg 1
Males
-5797 ± 0.2579
-6.131 ± 0.2517
Geno ype
0.3664
Females
-5.300 ± 0.1658
-5.307 ± 0.1657
Geno ype
0.9790
16
Bdn _Neg 1-/-
_Unpai ed_Welch’s es
-3.871 ± 0.05976
-4.091 ± 0.06628
Geno ype
0.0187
N k2(T kB)_Neg 1-/-
_Unpai ed_Welch’s es
-4.809 ± 0.1233
-4200 ± 0.1272
Geno ype
0.0015
qPCR Ta ge s
Bo h sexes
combined
P e on al co ex
Two-Way ANOVA
Synap ic adhesion
G oup housed
Social isola ion
Fac o s
G oup W
Mean ±SEM
( ans o med da a)
G oup Neg 1-/-
Mean ±SEM
( ans o med da a)
SI W
Mean ±SEM
( ans o med da a)
SI Neg 1-/-
Mean ±SEM
( ans o med da a)
X: In e ac ion
#: T ea men
$: Geno ype
F
( ans o med
da a)
P
( ans o med
da a)
Cn n1
-3.165 ± 0.06545
-3.328 ± 0.08302
-3.292 ± 0.06215
-3.398 ± 0.09169
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.1392
1.639
3.078
0.7102
0.2045
0.0836
N m1a
-3834 ± 0.1001
-4.047 ± 0.09233
-3.867 ± 0.1068
-4.079 ± 0.1120
Main e ec : $
In e ac ion
T ea men
Geno ype
Pos -hoc: -
9.272e-005
0.09962
4.275
0.9923
0.7532
0.0421
N m1b
-6.270 ± 0.1239
-6.340 ± 0.1016
-6.311 ± 0.1181
-6.352 ± 0.09842
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.01725
0.05597
0.2520
0.8959
0.8137
0.6174
Lsamp1a
-5.802 ± 0.1331
-5.738 ± 0.1142
-5.798 ± 0.1443
-5.765 ± 0.1404
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.01372
0.007523
0.1337
0.9071
0.9311
0.7157
Lsamp1b
-5.600 ± 0.08593
-5.694 ± 0.1028
-5.716 ± 0.1155
-5.686 ± 0.1061
Main e ec : -
In e ac ion
T ea men
Geno ype
0.3626
0.2766
0.09582
0.5489
0.6005
0.7578
17
Pos -hoc: -
Neg 1_Unpai ed_Welch’
s es
-5.536 ± 0.1570
-5.719 ± 0.1745
Geno ype
0.4396
qPCR Ta ge s
Bo h sexes
combined
Hippocampus
Two-Way ANOVA
Synap ic adhesion
G oup housed
Social isola ion
Fac o s
G oup W
Mean ±SEM
( ans o med da a)
G oup Neg 1-/-
Mean ±SEM
( ans o med da a)
SI W
Mean ±SEM
( ans o med da a)
SI Neg 1-/-
Mean ±SEM
( ans o med da a)
X: In e ac ion
#: T ea men
$: Geno ype
F
( ans o med
da a)
P
( ans o med
da a)
Cn n1
-2.343 ± 0.06799
-2.438 ± 0.06486
-2.374 ± 0.06194
-2.276 ± 0.05240
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
2.368
1.103
0.0006099
0.1282
0.2970
0.9804
N m1a
-4.162 ± 0.08780
-4.198 ± 0.08156
-4.140 ± 0.07082
-4.020 ± 0.06680
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
0.9984
1.668
0.2974
0.3209
0.2006
0.5871
N m1b
-5.701 ± 0.1056
-5.826 ± 0.08951
-5.682 ± 0.09063
-5.516 ± 0.06606
Main e ec : -
In e ac ion
T ea men
Geno ype
Pos -hoc: -
2.635
3.386
0.05335
0.1087
0.0697
0.8180
Lsamp1a
-4.105 ± 0.09293
-4.243 ± 0.07704
-4.064 ± 0.06735
-3.869 ± 0.06862
Main e ec : X##
In e ac ion
T ea men
Geno ype
Pos -hoc: **C l Neg 1-/- s SI Neg 1-/-
4.692
7.285
0.1371
0.0336
0.0086
0.7122
Lsamp1b
-4.526 ± 0.08712
-4.672 ± 0.07602
-4.539 ± 0.06738
-4.411 ± 0.06323
Main e ec : -
In e ac ion
T ea men
Geno ype
3.407
2.764
0.01518
0.0689
0.1006
0.9023
18
Pos -hoc: -
Neg 1_Unpai ed_Welch’s
es
-4.521 ± 0.1314
-4.509 ± 0.1243
Geno ype
0.9492
