The naming con en ion o he .czi iles is as ollows: he i s i e cha ac e s ep esen he
numbe o he animal (e.g., 03133); le o igh indica es hemisphe e; an e io , medial o
pos e io indica es sec ion; nex he ype(s) o s aining is (a e) indica ed, C esyl Viole (CV),
Myelin Basic P o ein (MBP), pa albumin (PV) o Neu onal Nuclea An igen and Neu on
Di e en ia ion Ma ke (NeuN) and when app op ia e he associa ed luo och ome (CY2, CY3 o
488). Fo 03133-le -pos e io he e a e 2 iles: 03133-le -pos e io _80_PV-CY3_MBP-488.czi &
03133-le -pos e io _81_PV-CY3.czi. Due o an a i ac he ex e nal ROI could no be analysed
on he o iginal sec ion (80); ins ead, i was analysed on he nex adjacen sec ion (81). All
sec ion iles con ain he anno a ions o he ROIs and laye s and can be opened in he Zen®
so wa e (Zeiss, Ge many) o in o he open sou ce so wa e.
The CSV ile, laye - hickness-CV.cs , con ains he measu ed hickness o each laye , o each
ROI, sec ion, hemisphe e and animal. The excel sp eadshee s con ain he minimum and
maximum cell diame e s measu ed o each laye , ROI, sec ion, hemisphe e and animal; each
cell ype being ep esen ed in a di e en shee . The e a e wo such iles: one measu ed om
cells s ained wi h C esyl Viole , cell-diame e -CV.xlsx, and he o he o pa albumin,
cell-diame e -PV.xlsx.
