In e na ional Con e ence on Bio ab ica ion 2023, Sep embe 17 h -20 h 2023, Saska oon, Canada
Cha ac e iza ion o mechanical p ope ies and
mic os uc u e o hyd ogel sca olds by X‑ ay
p opaga ion-based imaging
Nai ao Li1, Xiao Fan Ding1, Xiaoman Duan1, Ning Zhu1, 2, 3, Xiongbiao Chen1, 4
1Di ision o Biomedical Enginee ing, Uni e si y o Saska chewan, Saska oon, SK S7N 5A9, Canada.
2Canadian Ligh Sou ce, Saska oon, S7N 2V3, SK, Canada.
3Depa men o Chemical and Biological Enginee ing, Uni e si y o Saska chewan, Saska oon, SK S7N 5A9, Canada.
4Depa men o Mechanical Enginee ing, Uni e si y o Saska chewan, Saska oon, SK S7N 5A9, Canada.
Keywo ds — Tissue enginee ing, Hyd ogel sca old, Mechanical, Compu ed omog aphy.
INTRODUCTION
Cha ac e iza ion o he mechanical p ope ies and in e nal
mic os uc u e o sca old, once implan ed, is impe a i e in
issue enginee ing and medicine egene a ion. No ably, his
has been challenging o da e as a ious con en ional
cha ac e iza ion me hods by, o example, mechanical es ing
( o mechanical p ope ies) and mic oscope ( o in e nal
mic os uc u e) a e des uc i e as hey equi e emo ing
sca olds om he implan a ion si e and p ocessing samples
o cha ac e iza ion.
Synch o on adia ion X‑ ay p opaga ion-based compu ed
omog aphy (SR-PBI-CT) is easible and p omising o non-
des uc i e moni o ing o hyd ogel sca olds [1-2]. Wi h he
high b igh ness and high cohe ence o synch o on adia ion
sou ce, SR-PBI imaging is able o isualize and dis inguish
low-densi y so issues and hyd ogels wi h high spa ial
esolu ion and scan speed [1], while he ea u e o la ge
imaging dep h wi h X- ay CT ensu es he econs uc ion o
he h ee-dimensional (3D) sca old. As inspi ed, his s udy
aimed o pe o m a s udy on he cha ac e iza ion o
mechanical p ope ies and mic os uc u e o hyd ogel
sca olds by he SR-PBI-CT imaging.
MATERIALS AND METHODS
The bioma e ial solu ions we e p epa ed om 3% w/
algina e + 1% w/ gela in and hen p in ed by using he
needle wi h a diame e o 200μm, o o m sca olds wi h a
dimension o 10×10×5mm3. The p in ed sca olds we e
c osslinked in 100 mM/L calcium chlo ide solu ion o 48
hou s. Some o he sca olds we e deg aded in a 37°C media
o phospha e bu e ed saline o e 3 days, o he subsequen
examina ion, along wi h hose wi hou deg ada ion.
The sca olds bo h wi h and wi hou deg ada ion we e subjec
o comp essi e es ing on biodynamic sys em om BOSE,
whe e he comp ession speed was se a 0.1 mm/s o each
he s ain o 10%, 20%, 30%, 40%, and50%, espec i ely.
Once eached, he s ain was held o 5 minu es o measu ing
he o ce and hus he s ess in sca olds, yielding he s ess–
s ain cu es. Then, he SR-PBI-CT imaging we e pe o med
a he BMIT-ID beamline, Canadian Ligh Sou ce (CLS),
Canada. The sca olds we e loaded wi h he s ains as same
as comp essi e es ing and a each s ain, he sca old was
imagined and scanned wi h a pixel size o 13 µm o analyse.
RESULTS AND DISCUSSION
Fig. 1(a) shows he ep esen a i e o s ess-s ain cu e,
along wi h he s esses and Young’s moduli e alua ed e se
he s ain; while Fig. 1(b) shows he sca olds
mic os uc u es ea u ed by s and c oss-sec ion a ea, po e
size, and hyd ogel olume du ing comp ession. Ou esul s
ha e illus a ed ha he mechanical p ope ies and
mic os uc u es o sca olds, ei he being deg aded o no ,
can be examined and cha ac e ized by he SR-PBI-CT
imaging, in a non-des uc i e manne .
Fig. 1 Resul o bo h mechanical p ope ies and in e nal mic os uc u e o
he examined sca olds. S ess-s ain cu e and elas ic moduli (a). S and
c oss-sec ion a ea, po e size, and hyd ogel olume change du ing
comp ession (b).
CONCLUSION
The s udy e eals he po en ial o applying SR-PBI-CT o
isualize and cha ac e ize hyd ogel sca olds in e ms o hei
mechanical p ope ies and mic os uc u es in a non-des uc-
i e manne . This would ep esen a signi ican ad ance o
acili a ing longi ude s udies on he sca olds once implan ed
in i o.
ACKNOWLEDGEMENTS
This s udy was suppo ed by he Na u al Sciences and En-
ginee ing Resea ch Council o Canada (NSERC). Pa o e-
sea ch in his s udy we e pe o med a he Canadian Ligh
Sou ce (CLS).
REFERENCES
[1] Z. Izadi a .e al., Bioma e ials, ol. 82, pp. 151-167, 2016.
[2] L. Ning e al., ACS Applied Ma e ials & In e aces, ol. 13, no.
22, pp. 25611-25623, 2021.
