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Das et al. 2025 Source files for Westernblots

Lechtreck, Karl

Abstract

Das_etal2025_Source_files_WesternBlots The file contains the original uncropped images of Western blots, which will be published in "PKD2-like proteins target Chlamydomonas PKD2 to distinct subciliary regions" by Poulomi Das1, Haixia Zhou2, Nathan Chasen1, Jin Dai2, Alan Brown2, and Karl Lechtreck1# 1Department of Cellular Biology, University of Georgia, Athens, GA 30602 and 2Department of Biological Chemistry & Molecular Pharmacology, Harvard Medical School, Boston, MA 02115 Dasetal2025_MS_table_full Mass spectrometry analysis of control (g1) and pkd2 PKD2-miniT-TG samples (Mini). For each strain, eight samples were analyzed, of which seven were axonemal fractions (AXO), including two obtained from regenerating cilia ((REGEN), and one derived from the membrane and matrix (MM) fraction. Beads, samples were digested on the beads; Elute, proteins were eluted from the beads prior to analysis. The mass spectrometry source data are available at PRIDE (Project accession: PXD071255; Project DOI: 10.6019/PXD071255).

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PKD2-like proteins target Chlamydomonas PKD2 to distinct subciliary regions Poulomi Das1, Haixia Zhou2, Nathan Chasen1, Jin Dai2, Alan Brown2, and Karl Lechtreck1# Source files Figure 1A and Figure S1 Figure 1 A: 100KDa Anti-PKD2: 250KDa Anti-MST1: Anti-IC2: 75KDa 37KDa Anti-SIP: Figure 5A Anti-mNG Anti-mNG (dark; and anti-IC2, burned out) Anti-IC2 Anti-SIP Anti-IFT81 (left, right:anti-IC2) Anti-MST3 Anti-PKD2 rescue: Anti-MST1 rescue: Anti-IC2 rescue: Anti-MST1 rescue: 150KDa 100KDa 250KDa 150KDa 75KDa Figure S1 A: Figure S1 G: Anti-PKD2 (against loopdetecting both bands): 150KDa 100KDa Anti-MST1: 250KDa Anti-SIP: 100KDa Anti-PKD2 (against Cterminaldetecting one band only): 50KDa 35KDa Anti-IC2 :