Preparation and Evaluation of Simple Floral Fragrance

*Corresponding author: Kad Avishkar Suresh Copyright © 2025 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution License 4.0. Preparation and Evaluation of Simple Floral Fragrance Avishkar Suresh Kad 1, *, Prachi Nandkumar Padwal 2, Pratham Yogesh Bangar 1, Jayesh Gangadhar Jadhav 1, Sujal Anil Jagnade 1 and Ganesh Jayram Lamkhade 3 1 Samarth Institute of Pharmacy, Belhe, Pune, Maharashtra. 2 Department of Quality Assurance Technique, Samarth Institute of Pharmacy, Belhe, Pune, Maharashtra. 3 Department of Pharmaceutics, Samarth Institute of Pharmacy, Belhe, Pune, Maharashtra. World Journal of Biology Pharmacy and Health Sciences, 2025, 24(02), 118-124 Publication history: Received on 26 September 2025; revised on 02 November 2025; accepted on 04 November 2025 Article DOI: https://doi.org/10.30574/wjbphs.2025.24.2.0981 Abstract The aim of this research study was to formulate and assess a floral scent comprised of jasmine and mint essential oils, with the intention of establishing a light, refreshing, and well-balanced floral aroma. Ethanol was utilized as the primary solvent to facilitate mixing and controlled volatility, and glycerin was incorporated as an efficient fixative to promote fragrance persistence and stability. The proportions of top, middle (heart), and base notes was carefully optimized to achieve a well-blended floral-herbal fragrance [01]. The fragrance was assessed against important characteristics of pH measurement, odor stability during storage, and skin irritation testing to confirm safety and quality assurance. The fragrance demonstrated excellent stability, a pleasant aroma, and no skin irritation indicating a high potential for future use in various applications including perfumes, body sprays, creams and other cosmetic or personal care products [13]. Keywords: Floral Fragrance; Essential Oils; Jasmine Oil; Mint Oil; Ethanol; Glycerin; Fixative; Formulation; Aroma Stability; Cosmetic Product; Ph Evaluation; Odor Stability; Skin Irritation Test; Natural Fragrance; Perfume Formulation 1. Introduction Perfumes and fragrances have been an essential part of humanity since antiquity, synonymous with beauty, luxury, and emotional expression. Fragrances can be more than an aesthetic proposition; they can also be instrumental to personal hygiene and emotional well-being, by producing pleasurable sensations and encouraging relaxation [11]. Of the various categories of aroma, floral fragrances occupy a special place in the human experience, as their fragrances are naturally calming, elegant, and pleasing to all people. In contemporary drug and cosmetic fields, fragrances are often included as part of the composition of many products, including creams, lotions, soaps, deodorants, and hair care products [21]. Fragrances not only provide sensory appeal, they also enhance consumer satisfaction and acceptance of the product. The demand for natural and sustainable ingredients in cosmetics has risen tremendously in the last decade, and more people are interested to know if their fragrances are natural and derived from plant-based essential oils rather than a synthetic aromatic component. Essential oils derived from flowers such as jasmine, rose, lavender, and mint have been popularized for their nice aroma and other therapeutic effects. These plant-based extracts feature calming, stress-reducing, and mood-enhancing benefits in addition to usual hygiene effects for body and mind. Jasmine oil is a rich, sweet floral oil with relaxant and aphrodisiac properties, while mint oil has a refreshing and cooling top note that aids in enhancing floral top notes in a balanced aroma [17]. World Journal of Biology Pharmacy and Health Sciences, 2025, 24(02), 118-124 119 Figure 1 Floral Fragrance 1.1. Uses • Perfumes and Body Sprays o Act as the primary application for floral fragrances. o Provide a long-lasting pleasant scent that enhances mood and confidence. o Jasmine offers a warm, romantic floral tone, while mint adds freshness and cooling effects. o Suitable for both men’s and women’s fragrance blends. • Creams and Lotions o Improve the sensory appeal and user acceptance of moisturizing and skincare products. o Mask unpleasant odors from active ingredients. o Jasmine oil contributes to skin nourishment and helps relieve dryness, while mint oil provides a soothing, cooling sensation. • Soaps and Body Washes o Add a refreshing and luxurious aroma that enhances bathing experience. o Natural essential oils act as mild antiseptics and refresh the skin. o Jasmine helps in relaxation during bathing, and mint revitalizes the senses. • Deodorants and Antiperspirants o Provide long-lasting freshness and mask body odor. o Mint oil contributes antibacterial properties that help reduce odor-causing bacteria. o Suitable for both aerosol and roll-on formulations. • Hair Care Products (Shampoos, Conditioners, Hair Oils) o Add a pleasant fragrance that improves the sensory profile of hair products. o Jasmine oil strengthens hair roots and enhances shine. o Mint oil provides a cooling, scalp-soothing effect and can help reduce dandruff. • Aromatherapy and Stress Relief Products o Used in diffusers, candles, and massage oils for their calming and mood-enhancing effects. o Jasmine helps reduce anxiety and promotes relaxation. o Mint refreshes the mind, enhances alertness, and relieves fatigue. • Pharmaceutical and Therapeutic Preparations o Incorporated into topical formulations (ointments, balms, gels) to improve aroma and patient compliance. o Mint oil provides analgesic and cooling effects, beneficial in pain-relief balms. o Jasmine oil supports skin healing and may aid in mild anti-inflammatory effects World Journal of Biology Pharmacy and Health Sciences, 2025, 24(02), 118-124 120 2. Materials and Methods Table 1 Ingredients list Sr. No. Ingredient Quantity (% w/v) Function / Reason for Use 1 Jasmine Essential Oil 2% Provides a sweet, floral fragrance with calming and soothing effects; serves as the primary aroma component. 2 Mint Essential Oil 1% Adds a fresh, cooling note that balances the floral tone of jasmine; offers mild antimicrobial and refreshing properties. 3 Ethanol (Solvent) 90% Acts as a solvent to dissolve essential oils uniformly; helps in fragrance dispersion and quick drying when applied. 4 Glycerin (Fixative) 5% Serves as a fixative to slow down evaporation of volatile oils, enhancing aroma longevity and smoothness. 5 Distilled Water 2% Used to adjust the concentration and clarity of the formulation; ensures purity and stability. Table 2 Apparatus List Sr. No. Apparatus Purpose / Reason for Use 1 Beaker Used for mixing and blending the essential oils, solvent, and fixative during formulation. 2 Measuring Cylinder For accurately measuring the required volume of liquids such as ethanol, glycerin, and distilled water. 3 Funnel Helps in transferring the prepared fragrance solution into storage bottles without spillage. 4 Glass Rod Used for gentle stirring to ensure uniform mixing of all ingredients. 5 Dropper Allows precise addition of essential oils dropwise to control fragrance intensity. 6 Airtight Perfume Bottles Used to store the final fragrance formulation, preventing evaporation and contamination. World Journal of Biology Pharmacy and Health Sciences, 2025, 24(02), 118-124 121 2.1.1. Method of Preparation 2.2. Evaluation Parameters for Floral Fragrance Formulation Table 3 Evaluation Table S.No. Parameter Method Purpose 1 Odor Evaluation Sensory testing by a trained panel of evaluators. To assess the intensity, pleasantness, and floral harmony of the fragrance. 2 pH Measurement Using a calibrated digital pH meter at room temperature. Ensures formulation is within the skincompatible range and prevents irritation. 3 Stability Testing Store at different conditions (room temperature, 40°C, and sunlight) for a fixed duration. To check for changes in color, odor, or phase separation indicating instability. 4 Volatility Test Samples kept in open containers; evaporation rate monitored over time. To determine fragrance longevity and fixative efficiency. 5 Irritation Test (Patch Test) Apply a small amount on the forearm and observe for 24–48 hours. To assess skin compatibility and detect irritation or sensitization. 6 Color and Appearance Visual inspection under daylight. To ensure uniformity, clarity, and absence of particulate matter. 7 Viscosity Measurement Using a Brookfield viscometer at 25°C. To ensure uniform consistency and ease of application. 8 Microbial Stability Test Total viable count or spread plate method. To ensure product is free from microbial contamination and safe for use. 9 Compatibility with Packaging Store formulation in final packaging for 1– 3 months. To verify no interaction or leaching between product and container materials. 10 Retention Study (Longevity Test) Evaluate fragrance intensity over 6–8 hours post-application. To determine fragrance persistence and lasting effect on skin or fabric. World Journal of Biology Pharmacy and Health Sciences, 2025, 24(02), 118-124 122 3. Results The aromatic floral scent derived from the oils of jasmine and mint revealed to be an agreeable, fresh scent with a balanced and pleasant aroma. The odor assessment from the panel members denotes that the combination of sweet floral notes of jasmine and cooling freshness of mint together submit an agreeable and calming scent. The final pH of the formulation fell in the skin compatible range (−6.2), indicating suitability for topical/cosmetic use. For stability consideration, there were no estimable changes in color, clarity, or odor after a period of observation, which an indication of good physical and chemical stability. The volatility test indicated a moderate evaporation rate, indicating the functioning of the glycerin as a fixative to enhance the longevity of aroma. The irritation (patch test) did not show any signs of redness, itchiness, or irritation indicating that the formulation is safe for the skin. Overall, the results indicated that the floral scent formulation was stable, no irritation, and pleasant aroma with the capacity for making perfumes, creams, lotions, soaps and cosmetic products etc. 4. Discussion The formulation of a floral fragrance using jasmine and mint essential oils aimed to combine natural aromatic properties with stability, safety, and aesthetic appeal. The successful blending of these essential oils in ethanol, with glycerin as a fixative, demonstrated an effective and economical approach to fragrance preparation. The use of ethanol as a solvent ensured uniform dispersion of oils and a quick-drying effect upon application, which is desirable in perfumes and cosmetic products. Glycerin, being a hygroscopic compound, effectively reduced the volatility of the essential oils, thereby enhancing the retention and persistence of aroma over time.The odor evaluation by sensory analysis revealed a harmonious balance between the floral sweetness of jasmine and the fresh, cooling sensation of mint. This balance provided a pleasant and soothing fragrance profile, which aligns well with consumer preferences for natural and refreshing scents in cosmetic and toiletry products. The pH measurement confirmed that the formulation was within the skin-compatible range (around pH 6), indicating that it would not cause irritation or discomfort hen applied topically 5. Conclusion The present study successfully demonstrated the formulation of a natural floral fragrance using jasmine and mint essential oils with ethanol as a solvent and glycerin as a fixative. The developed fragrance exhibited a pleasant, refreshing, and long-lasting aroma, reflecting a balanced combination of floral and herbal notes. Evaluation parameters such as pH, odor stability, volatility, and irritation testing confirmed that the formulation was stable, skin-friendly, and safe for cosmetic use. The results indicated that glycerin effectively enhanced the fixation and longevity of the fragrance, while ethanol ensured uniform mixing and quick evaporation on application. Overall, the study concludes that a simple, cost-effective, and naturally derived fragrance formulation can serve as a viable alternative to synthetic perfumes, suitable for incorporation into cosmetic, toiletry, and aromatherapy products. Compliance with ethical standards Acknowledgments The author expresses his sincere gratitude to the Department of Pharmaceutics, Samarth Institute of Pharmacy, for providing the necessary laboratory facilities and materials to accomplish this research work. Special thanks to Ms.Prachi Nandkumar Padwal , Department of Quality Assurance, for her valuable guidance, motivation and continuous support during the project. I am very grateful to faculty members and laboratory assistants of the Department of Pharmacognosy for their help during herbal extraction and formulation studies. Disclosure of conflict of interest No conflict of interest to be disclosed. References [1] Satchell AC, Saurajen A, Bell C, Barnetson R StC. Treatment of interdigital tinea pedis with 25% and 50% tea tree oil solution: a randomized, placebo-controlled, blinded study. Australas J Dermatol. 2002;43(3):175–8. World Journal of Biology Pharmacy and Health Sciences, 2025, 24(02), 118-124 123 [2] Tong MM. Tea tree oil in the treatment of tinea pedis. J Am Acad Dermatol. 1992;26(5):790–2. [3] Mahmoud DA. Antifungal activity of different neem (Azadirachta indica) leaf extracts and their effect on human pathogenic fungi. J Pharm Clin Res. 2011;4(2):75–9. [4] Shrivastava DK, Swarnkar K. Antifungal activity of neem (Azadirachta indica) leaf extract. Int J Curr Microbiol Appl Sci. 2014;3(5):305–8. [5] Ogbolu DO, Oni AA, Daini OA, Oloko AP. In vitro antimicrobial properties of coconut oil on Candida species in Ibadan, Nigeria. J Med Food. 2007;10(2):384–7. [6] Hekmatpou D, Mehrabi F, Rahzani K, Aminiyan A. The effect of Aloe vera clinical trials on prevention and wound healing: a systematic review. J Educ Health Promot. 2019;8:65. [7] Ara K, Ono K, Hama M, et al. Foot odor due to microbial metabolism and its control. J Dermatol Sci. 2006;48(2):85– 92. [8] Al-Talib H, Al-Khateeb A, Mohd Zain N. Potassium aluminium sulfate (alum) inhibits growth of human axillary malodor-producing skin flora in vitro. J Clin Health Sci. 2016;1(1):23–9. [9] Alzomor AKY, Moharram AS, Sallam NM. Formulation and evaluation of potash alum as deodorant lotion, cream, and gel. Int Curr Pharm J. 2014;3(9):303–7. [10] Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol. 2004;94(3):223–53. [11] Souza ACA, Sobhy S. Phytochemical characterization and antifungal efficacy of camphor (Cinnamomum camphora) extracts. Molecules. 2023;28(4):1527. [12] Duda-Madej A, Kruk M, Fijalkowski K, Nowak D. Is camphor the future in supporting therapy for skin infections? A review. Dermatol Ther. 2024;37(2):e15842. [13] Unalan I, Cebeci FC, Kahraman E, et al. Physical and antibacterial properties of peppermint (Mentha piperita) essential oil formulations. J Appl Polym Sci. 2019;136(12):47281. [14] Singh R, Shushni MAM, Belkheir A. Antibacterial and antioxidant activities of Mentha piperita L. Arab J Chem. 2015;8(3):322–8. [15] Shino B, Peedikayil FC, Jaiprakash SR, Bijapur GA, Kottayi S, Jose D. Comparison of antimicrobial activity of chlorhexidine, coconut oil, and other natural antimicrobials against Candida albicans. J Int Soc Prev Community Dent. 2016;6(5):447–51. [16] Abdollahi A, Jafari SM, Naghdi M, et al. Enhanced antibacterial efficacy of camphor and thymol-loaded carriers: an in-vitro study. BMC Complement Med Ther. 2024;24(1):45. [17] Ogbolu DO, Oni AA, Daini OA, Oloko AP. In vitro antimicrobial properties of coconut oil on Candida species. Afr J Med Med Sci. 2007;36(4):421–4. [18] Massoud D, El-Sayed M, Nasr M. Aloe vera and wound healing: a brief review. Braz J Pharm Sci. 2023;59:e19378. [19] Arafa NMS, Ibrahim M, Hamad AA, et al. Potential of Aloe vera gel utilization for skin wound repair and cosmetology. Egypt J Basic Appl Sci. 2025;12(3):190–8. [20] Cortés N, Guzmán C, Parra J, Arancibia-Avila P. Innovative emulsifiers in cosmetic products: patents and trends. Pharmaceutics. 2024;16(1):150. [21] SpecialChem. Stearic acid: cosmetic ingredient INCI summary and uses. SpecialChem Industry Report. 2022. [22] Cosmetic Ingredient Review (CIR) Expert Panel. Safety assessment of triethanolamine and triethanolaminecontaining ingredients as used in cosmetics. Int J Toxicol. 2013;32(Suppl 1):1S–71S. [23] Silva NCC, Fernandes Júnior A. Essential oils and their major components: an updated review on antimicrobial activity. J Appl Microbiol. 2022;132(3):1593–617. [24] Araújo E, Silva M, Carvalho F, et al. Antifungal activity of neem leaf extract and combinations with other essential oils against dermatophytes. Phytother Res. 2023;37(5):1889–97. [25] Borade BR, More SD, Patil RS, et al. Formulation and evaluation of herbal antifungal cream. Int J Res Appl Sci Eng Technol. 2023;11(7):865–72. World Journal of Biology Pharmacy and Health Sciences, 2025, 24(02), 118-124 124 [26] Patankar PR, Chopade VV, Chaudhari PD. Foot deodorizing gel formulation having antimicrobial activity. J Res Pharm. 2023;27(3):1260–9. [27] Ara K, Hama M, et al. Identification of isovaleric acid and bacterial contributors to foot odor. J Dermatol Sci. 2006;48(2):85–92. [28] American Society for Microbiology (ASM). Microbial origins of body odor: overview and mechanisms. ASM News. 2021;87(4):112–7. [29] European Patent Office. Antiperspirant/deodorant composition containing alum in emulsions. EP3079648A1. 2016. [30] Al-Zomor AKY, Moharram AS, Sallam NM. Formulation and evaluation of potash alum as deodorant lotion, cream, and gel. Int Curr Pharm J. 2014;3(9):303–7.