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Unraveling the molecular basis of andromonoecy induction under heat stress conditions in Solanum melongena

Manrique, Silvia

Abstract

Climate change poses a significant challenge to global agriculture, and the Mediterranean region is particularly susceptible to its adverse effects. In this context, understanding plant responses to stress becomes imperative for mitigating yield losses. Several species of the genus Solanum, exhibit andromonoecy, a sexual system characterized by the coexistence of hermaphroditeand male flowers. Specifically, Solanum melongena displays functional andromonoecy, where“male” flowers possess a non-functional gynoecium because the reduced development of the stigma andthe style avoids the fertilization of the ovules. Under heat stress conditions, there is an observed increasein functionally-male flowers, suggesting that this increase is a mechanism to optimize resource allocationin response to environmental challenges. Our lab developed an early-flowering, dwarf eggplant variety,(“Micromel,”) more amenable for molecular studies. Utilizing Micromel plants, we performed transcriptomic and metabolomic analyses on pistils of flowers grown under control or heat stress conditions. Our findings reveal that heat stress induces autophagy, contributingto the increase of functionally-male flowers. Understanding the molecular mechanisms underlyingthe induction of functional andromonoecy in response to heat stress not only provides insights intoplant reproductive strategies but also offers avenues for enhancing stress tolerance in agricultural systems. By deciphering how plants allocate resources under stress, we can develop strategies to mitigate the impacts of climate change on plants both for agricultural and conservation purposes.

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Ref.613/3167 Unraveling the molecular basis of andromonoecy induction under heat stress conditions in Solanum melongena Silvia Manrique1, Gloria Villanueva1, Marina Martínez-López1, Miguel Chapa1, Mariola Plazas1, Pietro Gramazio1, Santiago Vilanova 1, Aureliano Bombarely2, Jaime Prohens1 1Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universitat Politècnica de València, Valencia, Spain 1Instituto de Biología Molecular y Celular (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC)/Universitat Politècnica de València, Valencia, Spain Introduction Results Pollen tubes fail to grow in short styles, leading to lack of fertilization, so short-styled flowers behave as male flowers pistil ovary style stigma PT PT = pollen tubes PT Long style Short style Thanks to Dolores Lerma,and Marina San Martín-Ramírez and Joan Sánchez-Pascual z for help with pollen analysis and to Ricardo Mir for microscopical equipment. This work was supported by Programa María Zambrano 2021 (to SM, UPV Ministerio de Universidades, Plan de Recuperación, Transformación y Resiliencia - Financiado por la Unión Europea – NextGenerationEU), PID2021-128148OB-I00 (MCIN/AEI/ 10.13039/501100011033 and “ERDF Away of making Europe”), PDC2022-133513-I00 (MCIN/AEI /10.13039/501100011033 and European Union NextGenerationEU/ PRTR), CIPROM/2021/020 (Generalitat Valenciana, Spain), FPU21/02288 (to MM-L; Spanish Ministerio de Universidades) and RYC2021-031999-I (to PG;MCIN/AEI /10.13039/501100011033 and the European Union through NextGenerationEU/PRTR)and Primeros Proyectos UPV (PAID-06-2023 to SM). Acknowledgements Long-styled flower Short-styled flower Flowers with short styles behave as male-only flowers as reduced development of stigma and style prevents ovule fertilization Eggplant displays functional andromonoecy In the presence of heat stress the amount of functionallymale flowers increases, leading to fertility reduction We have developed a dwarf, early flowering eggplant variety that facilitates molecular studies (Martínez-López et al., in preparation) MIcromel Control !25ᴼC, "20ᴼC Heat stress !35ᴼC, "30ᴼC DAS = days after stress Micromel shows the same response to heat stress regarding andromonoecy as other eggplant varieties. The response pattern suggests it might be an adaptation to resource limitation Heat stress (!35ᴼC, "30ᴼC) Control (!25ᴼC, "20ᴼC) T1 T2 T3 Control Heat stress (enrichment) Transcriptomic analysis of pistils in control and heat stress conditions reveals extensive metabolic reprogramming along with changes in other ”house-keeping” processes like translation of photosynthesis (enrichment) UP-REGULATED DOWN-REGULATED KEGG PATHWAYS ENRICHMENT ANALYSIS (T3) LOW ENERGY SYNDROME LOW ENERGY SYNDROME Modified from Tome et al., 2014 ⇈⇈ TAKE-HOME MESSAGE: ▪Transcriptomic data shows that plants are adapting to low energy conditions caused by heat stress, aprocess regulated by TOR and SnRK1pathways. ▪In this context, the transient increase in andromonoecy would be part of this “low energy syndrome” contributing to plant adaptation to stress conditions Transcriptomic analysis sampling