Self-Assembling Lipopeptide Hydrogels as Functional Scaffolds for Tissue Regeneration

PROGRAM AND BOOK OF ABSTRACTS 3 - 5 December 2025 Campus CNRS, Pôle Balard, Montpellier https://scf-gs2025.sciencesconf.org/ JOURNÉES SCF GRAND SUD 2025 JOURNÉES SCF GRAND SUD 2025 SCF and RJ-SCF Occitanie-Méditerranée Detailed program 11:35 OC50 Marie JOYEUX IBMM - Montpellier Synthesis and Biological Evaluation of DNA Decoys Bearing Modified Internucleosidic Linkages OC57 Nathan MICHEL ICGM - Montpellier Sustainable Synthesis of Non-Isocyanate Polyurethanes from Biobased Phenols and N,N-disubstituted Formamides via Copper Catalysis OC64 Sedik ILLOUL ICSM - Marcoule Palladium recovery from electronic waste with malonamide-impregnated supports 11:50 OC51 Pierre PLAZA-Joly IEM - Montpellier Design and synthesis of alumina-supported Prussian blue analogue for efficient NH adsorption 3 OC58 Laura NICOLLET IBMM - Montpellier Ligation of oligonucleotides induced by Charge-Transfer interactions OC65 Anne CLISSON Univ Côte d'Azur - Nice Gold(I)-Catalyzed Enantioselective Cycloisomerization of 1,6-Oxygenated Enynes: an Entry to Hedonic Derivatives 12:10 - 13:00 PL7 Victor MOUGEL - Transforming Essential Small Molecules Through Bio-inspired Approaches Chair: Raphaël El Bekri Saudain 13:00 - 14:00 Lunch & Posters session 14:00 - 15:00 Oral communications session #9 Oral Communications Session #9A Balard Amphitheatre Chairs: Xavier Bantreil - Adrien Gallego Oral Communications Session #9B Balard 1 Chair: Armelle Ouali - Michael Carboni Oral Communications Session #9C DR13 Amphitheatre Chair: Marie Lopez - Jordan Lehoux 14:00 OC66 Léa JOLY ICGM - Montpellier N-type and p-type organic materials for thermoelectric applications OC70 Patrizia RUSSO IBMM - Montpellier Disaggregation Strategies and Source-Dependent Behavior of Aβ42: A Taylor Dispersion Analysis Approach OC74 Yves-Marie LEGRAND ChimEco - Montpellier Multistep green synthesis of BODIPY fluorescent dyes using ecocatalysis® 14:15 OC67 Dhriti SHENOY IBMM - Montpellier Self-Assembling Lipopeptide Hydrogels as Functional Scaffolds for Tissue Regeneration OC71 Ranim GARROUCH ICGM - Montpellier Tanning and Functionalization of Leather by Sol-Gel Process OC75 Chunyan XIANG ICGM/IEM - Montpellier Chirality-Induced Spin Selectivity Enables Efficient Urea Oxidation on NiCo-LDHs 14:30 OC68 Ioan STROIA IEM - Montpellier CH vs. NH vs. Synergistic NH/OH Donors: A Lesson of Selectivity in Anion Transmembrane Transport OC72 Jean ROUVEYROLLES ICGM - Montpellier Novel chloride electrolytes for high-performance sodium solid-state batteries OC76 François SALBERT IBMM - Montpellier New water-soluble peptide aromatic foldamers: synthesis, characterization and potential applications 14:45 OC69 Naoual BAKHROU ICGM - Montpellier Tandem Rhodium-Catalyzed C-O Bond Cleavage of Lignin Models Followed by Copper-Catalyzed Biarylether Synthesis OC73 Leon SCHÜTTE ICGM - Montpellier In-situ characterization of Tetragonal Na Si Phase under High Pressure-HighTemperature 2 3 OC77 Tao YANG ICGM - Montpellier Racemic & Enantioselective copper-catalyzed insertion of α-diazoalkanes into Nu-H bond: P-S-C(*)-CF , P-S-C(*)-P bond formation 3 15:00 - 15:50 PL8 Cédric-Olivier TURRIN Anti-inflammatory dendrimers. The life cycle of a research program, from academic research to preclinical development within a biotech company Chair: Marie Lopez - Jordan Lehoux 15:50 - 16:00 Poster & oral communication awards 16:00 Closing ceremony Self-Assembling Lipopeptide Hydrogels as Functional Scaffolds for Tissue Regeneration Authors – Dhriti Shenoy1,2, Michela Pesenti1, Kaliroi Peqini1, Emeline Perrier-Groult3, Cécile Echalier2, Gilles Subra2, and Sara Pellegrino1 1 Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, Italy 2 IBMM, University of Montpellier, CNRS, ENSCM, France 3 IRMB, University of Montpellier, INSERM, CHU Montpellier, France Email of the presenting author [email protected] Lipopeptides are a class of bio-inspired, self-assembling peptides amphiphiles that have attracted significant attention over the past decade due to their biocompatibility and self-organization into diverse nanostructures in response to stimuli. These materials often exhibit both thermodynamically and kinetically driven self-organization, leading to the formation of hierarchically ordered nanostructures with specific properties. 1 Here, we developed lipopeptide-based hydrogel designed to support tissue regeneration. The hydrogel architecture comprises of 3 domains: a hydrophobic lipid (stearic acid) tail that drives the self-assembly, a central gelation peptide (FQFQFK) that stabilizes the gel matrix, and a terminal bioactive peptide (GFOGER) that promotes cellular interaction. While the FQFQFK sequence facilitates gel formation through β-sheet-driven selfassembly2, the stearic acid moiety helps to improve the structural stability and gel stiffness3. Meanwhile, the bioactive GFOGER motif, derived from collagen, is incorporated to enhance cell adhesion, osteogenesis, chondrogenesis, and mineralization, making the hydrogel well-suited for regenerative medicine applications4. This modular design enables precise control over self-assembly, structural integrity and bioactivity. The resultant hydrogel represents a versatile platform for the development of nanostructured scaffolds with tunable properties achievable through sequence or lipid modification. These findings highlight the potential of lipopeptide-based hydrogels as customizable materials for regenerative medicine and tissue engineering applications. Figure: Design of bioactive scaffolds for tissue regeneration using lipopeptide hydrogels Acknowledgements This project has received funding from the European Union’s research and innovation programme under the Marie Skłodowska-Curie grant agreement No 101072645 1 W. Hamley, I, Chem. Commun. 2015, 51, 8574-8583. 2 Bertouille J, et al.Mater. Today Chem. 2025, 44, 102593. 3 Almeida M., et al. Journal of Colloid and Interface Science, 2025, 639, 137571. 4 Ha, M.Y., Yand, D.H., You, S.J. et al. Regen Med, 2023, 8, 2. OC67 Self-assembling lipopeptide hydrogels as functional scaffolds for tissue regeneration SCF Grand Sud 2025 Dhriti Shenoy Supervisors: Prof. ssa Sara Pellegrino (UniMi) Prof. Gilles Subra and Dr. Cécile Echalier (UM) THE PROBLEM CURRENT SOLUTION THE CHALLENGE Background Inability of tissue to heal Synthetic tissue scaffold Mimicking the native tissue environment High biocompatibility-short protein fragments Peptide materials Easy to modify bioactivity Sequence modification-tune structural, confirmational & chemical properties AIM To design, synthesize and characterize novel bioactive peptide nanomaterials for applications in tissue engineering Sequence engineering To use N-terminal modification strategy to obtain bioactive peptide hydrogels BIOACTIVE PEPTIDE SELF-ASSEMBLING SEQUENCE Sequence engineering To use N-terminal modification strategy to obtain bioactive peptide hydrogels BIOACTIVE PEPTIDE SELF-ASSEMBLING SEQUENCE GFOGER Peptide Synthesise Microwave-assisted Solid Phase Peptide synthesis Peptide Synthesise Microwave-assisted Solid Phase Peptide synthesis Peptide hydrogel Peptide sequences Peptide conc. For hydrogel (w/v%) Hydrogel Stearic acid -FQFQFKGFOGER-NH22% FQFQFKGFOGER -NH26% Stearic acid -FQFQFKGFPGER-NH24% FQFQFKGFPGER -NH26% Circular Dichroism Sample is analysed using circularly polarized light. Light interacts with peptide bond and is refracted based on associated structure Circular Dichroism Rheology All hydrogels were tested at 6% w/v concentration. The following tests were done: 1. Frequency sweep at 1% strain and 37°C 2. Amplitude sweep at 1Hz frequency and 37°C 3. Time sweep at 1Hz frequency, 1% strain and 37°C 4. Flow sweep at 37°C Rheology All hydrogels were tested at 6% w/v concentration. The following tests were done: 1. Frequency sweep at 1% strain and 37°C 2. Amplitude sweep at 1Hz frequency and 37°C 3. Time sweep at 1Hz frequency, 1% strain and 37°C 4. Flow sweep at 37°C Rheology Cytotoxicity(LDH assay) Mouse MSC culture Well-plates with inserts Plate reader Assay result Insert containing hydrogel Well seeded with mouse MSCs Cytotoxicity(LDH assay)