Inclusion of carotenoids in broodstock diets for gilthead seabream

-:ro ISfNF May 28 - June 1, 2006 Biarrilz, France lIlARRITZ :200b INCLUSION OF CAROTENOIDS IN BROODSTOCK DIETS FOR GILTHEAD SEABREAM (Sparus 4urata L .. 1758) : EFFECTS ON EGG ANO SPAWNLNG QUALlTY Scabini, V. IJ , Fermíndez-Palacios, H.· and Izquierdo. M.S! I Grupo de Investigación en Acuicultura. P.O. Box 56. 35200. Telde, Las Palmas. Canary Islands, Spain. 2 Facultad de Ciencias, Un i\'ersidad de Magaltanes, Punta Arenas, CHILE Carotenoids are known to be necessary in diclS fOf broodstock of sc"cral specics including sparids. Howcver, thi~ al>pt:!,;l uf brouúslol;k nuLriliun hiClll lIul lx::t:n yl:l ~ludil;:d ¡1I :'1."llLrt:alll. TIIU:', 1I,]!: ubjl;:~livl;: uf 1111: pl1:l>1:1I1 experiment 'Nas lo invcstiga le the ctlecl 0 1' supplementation 01" practical diels tor gilthead seabream wi th two different camten(1id_~ concentration levels (30 and 60 mgfkg fmm a paprika source) in combinalion with tv.',1 n-3 HUFA lc\-cls (2.5 and 4 % dry wcight, from fish oil) on cgg and spawning quulity. Thirty si" matUTe scabrearns (Spams a:/r(J(a L., 1758) of 1.20 kg average body wdght fur th t: females and 0.460 kg lar th e males wert: divided i nlO 12 groups of 2 males and 1 lemale in I m3 tibre tanks and fed onc 01' ¡he Ibllowing diets: 3012.5 (30 mglkg total carote"loids Md 2.50/0 HUFA), 30 /4 (10 mg/lcg tOlal carolenoids anrl4.0 HU FA), 60 /2.5 (60 mglkg total carotcnoids flnd 2.5 HUFA) flnd 601 4 (60 mg/kg 10lel cflrolenoids and 4 HUFA). Each diet was tested by triplicates. Fish were fed three times a day for 7 wceks l.~% bodyweightlday. Fi ve spawning qualit}' paramelers were daily recorded for 30 da) ·s . 8esides, samples ofeggs were taken alonj! me c:\perimental period and analyzed for proximale aOO fally atid composilion. After 60 days of feeding, spawning quality was signilicanlly higher in Ih e broodslock fed 60 /4 die!. In average, along !he wholc fccding pcriCKI egg viability and halching mtes, together wilh total number of viable eggs, jusI hatched out larvae and number ofalive lanae al mouth op~n : ng were significantly higher lar broodstock fed this 60/4 diel. Elevation of either carotenoids content of n-3 HUFA, only sl ighlly, but nol significanlly improved cenain sp awning quality puameters. Analysis showed that earoleno id s from paprika wcre succcssfully incorporatcd inlo eggs, suggcsting the good utilization of this ingredient by gilthead seabream broodstod:: and its effectiveness lo improve sp~""ning quality in this species. Kcywords : carotenoids; broodslock ; spawning; quality ; HU), '/\.