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Effect of pH on the production of hydrolytic enzymes of industrial interest by Bacillus licheniformis using sewage sludge as a low-cost culture médium

Rodríguez Morgado, Bruno; Caballero Jiménez, Pablo; Carballo Álvarez, Modesto José; Tejada Moral, Manuel; Aragón Cruz, Carlos Alberto; García Martínez, A.; Salas Liñán, Joaquín Jesús; Parrado Rubio, Juan

Abstract

Management of sewage sludge has become a topic of increasing importance. This is because the treatment and final destination of these wastes are not growing at the same rate at which generated what is necessary to add the limited storage capacity of wastewater treatment plants or waste transfer centers. Current policies of different governments aims, among others, promote proecological management of sewage sludge, replacing traditional methods by new methods that lead to their stabilization and safe recycling. These methods can lead to the recovery of valuable raw materials from potentially dangerous others in order to permit their use in agriculture, industry or energy production. Moreover, exists a great enzyme market, mainly those with hydrolytic activities such as proteases, lipases or cellulases. Although its use has many advantages, the competitiveness of the enzymes compared with chemicals is limited by its high production costs. The use of a less expensive culture media and the stimulation of the production of these enzymes can significantly reduce production costs. Therefore, the use of tertiary materials as alternative substrates, available in large quantities and at lower cost, is an interesting option. In this work we have studied the effect of pH on the fermentation of sewage sludge by Bacillus licheniformis, a hydrolytic enzymes producer microorganism, in order to obtain several products for specific agronomic applications.

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Page 81 Beneficial Microbes 2015 August 25-27, 2015 Notes: August 25-27, 2015 Valencia, Spain World Congress on Beneficial Microbes: Food, Pharma, Aqua & Beverages Industry Volume 3 Issue 2 Ferment Technol 2015 ISSN: 2167-7972, FMT an open access journal Effect of pH on the production of hydrolytic enzymes of industrial interest by Bacillus licheniformis using sewage sludge as a low-cost culture medium Bruno Rodríguez-Morgado1, P. Caballero1, M. Carballo2, M. Tejada3, C. Aragón4, A. García-Martínez4, J.J. Salas4, J. Parrado1 1Departamento de Bioquímica y Biología Molecular, Universidad de Sevilla. C/Profesor García González 2, 41012 Sevilla. 2Centro de Investigación, Tecnología e Innovación de la Universidad de Sevilla. Edificio Celestino Mutis. Avda. Reina Mercedes, s/n. 41012 Sevilla. 3Departamento de Cristalografía, Mineralogía y Química Agrícola, Universidad de Sevilla, Cta. Utrera Km 1. 41089 Sevilla. 4Fundación Centro de las Nuevas Tecnologías del Agua. Autovía Sevilla-Huelva (A-49), Km. 2841820 Carrión de los Céspedes (Sevilla).Spain Management of sewage sludge has become a topic of increasing importance. This is because the treatment and final destination of these wastes are not growing at the same rate at which generated what is necessary to add the limited storage capacity of wastewater treatment plants or waste transfer centers. Current policies of different governments aims, among others, promote proecological management of sewage sludge, replacing traditional methods by new methods that lead to their stabilization and safe recycling. These methods can lead to the recovery of valuable raw materials from potentially dangerous others in order to permit their use in agriculture, industry or energy production. Moreover, exists a great enzyme market, mainly those with hydrolytic activities such as proteases, lipases or cellulases. Although its use has many advantages, the competitiveness of the enzymes compared with chemicals is limited by its high production costs. The use of a less expensive culture media and the stimulation of the production of these enzymes can significantly reduce production costs. Therefore, the use of tertiary materials as alternative substrates, available in large quantities and at lower cost, is an interesting option. In this work we have studied the effect of pH on the fermentation of sewage sludge by Bacillus licheniformis, a hydrolytic enzymes producer microorganism, in order to obtain several products for specific agronomic applications. Biography Bruno Rodríguez Morgado is a PhD student with a experience of eight years in enzyme production by microbes and the production of enzymatic extracts of several agroindustrial by-products like feathers, sewage sludges, grape pomace, etc. [email protected] Bruno Rodríguez Morgado, Ferment Technol 2015, 3:2 http://dx.doi.org/10.4172/2167-7972.S1.003