Determinants of blood eosinophil levels in the general population and patients with COPD: a population-based, epidemiological study

Miravitllesetal. Respiratory Research (2022) 23:49 https://doi.org/10.1186/s12931-022-01965-3 RESEARCH Determinants ofblood eosinophil levels inthegeneral population andpatients withCOPD: apopulation-based, epidemiological study Marc Miravitlles1,2* , Juan José Soler‑Cataluña2,3, Joan B. Soriano2,4, Francisco García‑Río2,5, Pilar de Lucas6, Inmaculada Alfageme7, Ciro Casanova8, José Miguel Rodríguez González‑Moro9, M. Guadalupe Sánchez‑Herrero10, Julio Ancochea2,4 and Borja G. Cosío2,11 Abstract Background: Blood eosinophils are considered a biomarker for the treatment of chronic obstructive pulmonary disease (COPD). Population‑based studies are needed to better understand the determinants of the blood eosinophil count (BEC) in individuals with and without COPD. Methods: EPISCAN II is a multicentre, cross‑sectional, population‑based epidemiological study aimed at investigating the prevalence and determinants of COPD in Spain. Study subjects were randomly selected from the general popula‑ tion, and COPD was defined by a post‑bronchodilator FEV1/FVC < 0.7. For the pre‑specified outcomes related to BEC, the first 35 COPD and 35 non‑COPD subjects were consecutively recruited in 12 of the participating centres with the objective of analysing 400 individuals in each group. Baseline BEC and its association with demographic, clinical and functional variables were analysed. Results: A total of 326 COPD and 399 non‑COPD subjects were included in the analysis. The mean age (standard deviation [SD]) was 63.2 years (11.0), 46.3% were male, and 27.6% were active smokers. BEC was significantly higher in individuals with COPD [192 cells/μL (SD: 125) vs. 160 cells/μL (SD: 114); p = 0.0003]. In a stepwise multivariate model, being male, active smoker and having a previous diagnosis of asthma were independently associated with having a higher BEC. Conclusions: This population‑based study estimated the distribution of eosinophils in the healthy adult population and concluded that COPD patients have a significantly higher BEC. Male sex, active smoking and concomitant asthma were significantly associated with a higher BEC. Keywords: Epidemiology, Computed tomography, Imaging population, Biomarkers © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/. The Creative Commons Public Domain Dedication waiver (http:// creat iveco mmons. org/ publi cdoma in/ zero/1. 0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Background Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease with multiple phenotypes and endotypes, which may be associated with different prognoses and response to treatment [1, 2]. One of the most widely studied phenotypes of COPD is that associated with increased eosinophilic inflammation. Although there is Open Access *Correspondence: [email protected] 1 Pneumology Department, Hospital Universitari Vall d’Hebron/Vall d’Hebron Institut de Recerca (VHIR), Vall d’Hebron Barcelona Hospital Campus, P. Vall d’Hebron 119‑129, 08035 Barcelona, Spain Full list of author information is available at the end of the article Page 2 of 10 Miravitllesetal. Respiratory Research (2022) 23:49 still controversy about the role of the blood eosinophil count (BEC) as a marker of risk of exacerbation [3–8], there is wide consensus about the role of the BEC as a biomarker to identify patients who will present better response to inhaled corticosteroids (ICS) [9–11]. In fact, the most recent recommendations of pharmacologic treatment include the BEC as a guide for the indication of treatment with ICS, particularly in patients at risk of exacerbations [12, 13]. In order to better understand the role of blood eosinophils as a biomarker in COPD it is important to generate new data about the distribution of blood eosinophil values in healthy adult populations and different groups of patients with COPD according to different phenotypes, degree of severity and treatments. While the distribution of blood eosinophils has been examined in several United States [3, 14] or North and Central Europe-based COPD populations [14–18], data from Southern European countries are limited. EPISCAN II is a population-based, epidemiological study the main objective of which was to investigate the prevalence and determinants of COPD in all autonomous communities in Spain [19]. One of the pre-specified secondary objectives of the EPISCAN II study was to analyse the distribution of BECs in a subsample of participants with and without COPD in order to estimate BECs in the general adult Spanish population and to investigate factors associated with an elevated BEC [19]. The current article presents the results of the analysis of BECs and their determinants in a subgroup of participants in the EPISCAN II study with and without COPD from centres distributed throughout Spain. Methods EPISCAN II is a national, multicentre, cross-sectional, population-based epidemiological study the main objective of which was to investigate the prevalence of COPD in Spain. The full protocol, the fieldwork and all the methods have been described previously [19, 20]. Briefly, 20 hospitals from the 17 Spanish autonomous communities conducted the study from April 2017 to February 2019. Subjects from the general population who were resident in the postal code areas nearest the participating hospitals were selected. A list of random telephone numbers was obtained, stratified according to these postal codes and quotas for sex and age groups. The inclusion criteria were as follows: men or women aged 40years or more with no physical or cognitive difficulties that would prevent them from completing spirometry or any of the study procedures. The study was approved by the ethics committees of each of the participating centres, and all participants provided informed consent. The EPISCAN II protocol is registered at https:// clini caltr ials. gov (NCT03028207) and at www. gskclini calst udyre gister. com/ study/ 205932. Variables andprocedures Demographic information on age, sex, level of education, family conditions, comorbidities, weight, height and smoking were collected. The number of respiratory exacerbations in the last 12months was also collected. Forced spirometry was performed using a pneumotachograph (Vyntus Spiro, Carefusion, Germany), according to standardized procedures [21], and Global Lung Function Initiative equations were used as the reference value [22]. Spirometry was performed before and 15–30min after inhalation of 400μg of salbutamol. COPD was defined as a post-bronchodilator forced expiratory volume in one second/forced vital capacity (FEV1/FVC) ratio < 0.7. The study population was divided into two cohorts, depending on the results of the post-bronchodilator spirometry: patients with COPD (FEV1/FVC < 0.7) and non-COPD individuals (FEV1/FVC ≥ 0.7). Symptoms were assessed by the COPD Assessment Test (CAT) questionnaire. Physical activity was measured by the Yale Physical Activity Questionnaire (YPAS) validated for the Spanish population and the elderly population. This questionnaire reflects the amount, frequency, and intensity of physical activity that can be used to estimate the effects of physical activity as a continuous parameter, even at the low levels of activity that might be expected in COPD patients [23, 24]. The 6-min walking test (6MWT) was performed according to the American Thoracic Society (ATS) guidelines [25]. Absolute values and percentage of diffusing capacity of the lung for carbon monoxide (DLCO) and alveolar volume were collected by single-breath CO diffusing capacity (MasterScreen diffusion, Carefusion, Germany), according to the ATS/European Respiratory Society (ERS) recommendations [26]. Twenty ml of venous blood were collected from each participant for routine blood analysis, and determination of C-reactive protein (CRP) and fibrinogen. The collection procedure was standardised, and each centre stored the samples at − 80°C until shipping for centralised analysis. Computed tomography (CT) images were acquired during inhalation, without contrast and with lowdose radiation. The images obtained underwent semiautomatic post-processing for determination of the Page 3 of 10 Miravitllesetal. Respiratory Research (2022) 23:49 percentage of emphysema, areas of extension, airway thickness, and other lung parenchyma attenuation and airway wall thickness parameters [27–29]. Statistical analysis Considering the 10.2% prevalence of COPD found in the previous EPISCAN study [28] a priori sample size calculation estimated that with an accuracy of ± 0.6% and a 10% dropout rate, approximately 10,200 eligible individuals should be included in the study for the primary outcome, that is, the prevalence of COPD in Spain. For secondary outcomes related to CT scan and blood analysis, the first 35 COPD and 35 non-COPD subjects were consecutively invited to participate in 12 of the participating centres with the objective of recruiting a total of approximately 400 individuals in each group. Categorical variables were presented as numbers with percentages, and continuous variables as mean with standard deviation (SD). The characteristics of the population in the diagnosis of COPD groups were compared using the Student’st-test, or χ2test. The comparisons of blood eosinophil levels according to clinical, functional and CT characteristics were tested for significance using the Student’st-test and analysis of variance. Correlations between continuous variables were evaluated using Pearson’s correlation coefficient. Univariate and multivariate linear regression analyses were used to investigate factors associated with blood eosinophil levels. Only variables with a level of significance < 0.1 in the univariate analysis were included in a stepwise multivariate linear regression model. The R2 coefficient of determination was calculated for the model. Data were analysed with the Statistical Analysis System (SAS) Enterprise Guide 7.15, considering a statistical significance (p) of 0.05 for all the statistical tests performed. Results Population The 12 participating centres recruited a total of 326 COPD and 399 non-COPD subjects, who constituted the population of our study. The mean age of the participants was 63.2years (SD): 11.0, and 46.3% were male, and 27.6% were active smokers. Most clinical characteristics were significantly different between COPD and non- COPD participants. COPD subjects were older, more frequently male, less frequently never smokers, with lower lung function parameters, less exercise tolerance and higher CAT scores (Table1). Regarding blood analysis, COPD individuals had no significant differences in red blood cells, platelets, fibrinogen (g/L) or CRP (mg/dL) values but did have higher concentrations of total leukocytes, neutrophils and eosinophils (p < 0.05) (Table1). On CT imaging, COPD participants showed a higher percentage of areas of emphysema [10% (11.9) vs. 4.7% (7.5); p < 0.0001] and higher percentages of airway wall area in both primary and secondary bronchi (both p < 0.0001) (Table1). Blood eosinophil counts according tothedifferent subject characteristics The mean BEC was 175 cells/μL (SD: 120; 95% confidence interval [CI]: 166;184) and median 150 cells/ μL. BECs were significantly higher in individuals with COPD (192 cells/μL (SD: 125; median: 177 cells/μL) vs. 160 cells/μL (SD: 114; median: 132 cells/ μL); p = 0.0003). The distribution of blood eosinophils in both groups of subjects is depicted in Fig.1. There were no differences in the proportion of subjects with more than 300 eosinophils/μL (11.5% in non-COPD vs. 16% in COPD, p = 0.08), but there were more non-COPD subjects with less than 100 eosinophils/μL compared with COPD patients (32.8% vs. 20.6%, p = 0.0002). In the 159 subjects with airflow obstruction according to the lower limit of normal of FEV1/FVC the mean BEC was 204 cells/μL (SD: 135; 95% confidence interval [CI]: 182;225) and median 180 cells/μL. BECs were also significantly higher in men, smokers, individuals with more advanced stages of COPD and in those with a previous diagnosis of asthma. The differences in BEC according to CT scan parameters did not achieve statistical significance (Table2). This analysis was repeated separately for the COPD patients and the non-COPD participants, and neither subgroup showed significant differences in BEC between individuals with or without significant emphysema or airway thickness (data not shown). Since the majority of the COPD patients identified were mild, we changed the cut off of emphysema from 10 to 5%, but again no significant differences in BEC were observed (189.5 cells/μL (SD: 134.2) in patients with 0–5% emphysema compared to 202.8 cells/μL (SD: 129.1) in patients with > 5% emphysema; p = 0.18). Figure2 shows the mean and 95%CI of blood eosinophil levels in the different subgroups of participants classified according to the variables showing significant differences in univariate analysis. Page 4 of 10 Miravitllesetal. Respiratory Research (2022) 23:49 Factors independently associated withBEC A stepwise multivariate model was developed with the following variables: age, sex, body mass index, smoking, previous diagnosis of asthma, COPD, CAT scores, FEV1 (%), DLCO (%), YPAS score, 6MWD, exacerbations, ICS treatment, platelets, fibrinogen, CRP, emphysema (% fixed threshold), and airway wall area (for central and peripheral airways in %). Only five variables had a p value < 0.10 in the final model, with three being significant at a level of < 0.05. Being male, active smoker, and having a previous diagnosis of asthma were significantly Table 1 Demographic, clinical, blood, and imaging characteristics of participants according to the presence of airflow limitation compatible with COPD BMI body mass index, CAT COPD assessment test, FEV1 forced expiratory volume in the first second, FVC forced vital capacity, DLCO diffusion capacity of the lung for carbon monoxide, 6MWD 6-min walk distance, YPAS Yale Physical Activity Questionnaire, CRP C-reactive protein COPD (n = 326) Non-COPD (n = 399) All (n = 725) P value Demographic Age, years 66.9 (10.5) 60.1 (10.5) 63.2 (11.0) < 0.0001 Sex, male, % 182 (55.8%) 154 (38.6%) 336 (46.3%) < 0.0001 BMI 27.1 (4.4) 27.1 (4.8) 27.1 (4.6) 0.986 Smoker 114 (35.0%) 86 (21.6%) 200 (27.6%) < 0.0001 Former smoker 151 (46.3%) 152 (38.1%) 303 (41.8%) Never smoker 61 (18.7%) 161 (40.4%) 222 (30.6%) Clinical Asthma diagnosis 42 (12.9%) 32 (8.0%) 74 (10.2%) 0.031 CAT 9.2 (6.9) 7.0 (5.9) 8.0 (6.5) < 0.0001 FEV1, L 2.2 (0.7) 2.9 (0.7) 2.6 (0.8) < 0.0001 FEV1, % 82.0 (18.7) 104.6 (14.6) 94.4 (20.0) < 0.0001 DLCO, % 89.9 (23.1) 98.8 (18.4) 94.8 (21.1) < 0.0001 FEV1/FVC 62.0 (8.4) 79.8 (5.1) 71.8 (11.1) < 0.0001 6 MWD, m 478 (108) 518 (98) 501 (105) < 0.0001 YPAS, score 45.7 (23.1) 48.4 (21.6) 47.2 (22.3) 0.111 Exacerbations last year 0.27 (0.82) 0.05 (0.27) 0.14 (0.60) < 0.0001 Treatment with ICS 61 (18.7%) 16 (4.0%) 77 (10.6%) < 0.0001 Blood Erythrocyte, cells/µl 4.95 (0.52) 4.92 (0.44) 4.93 (0.47) 0.796 Leukocyte, cells/µl 7273 (2194) 6746 (1838) 6983 (2022) 0.0005 Neutrophils, cells/µl 4284 (1603) 3925 (1426) 4086 (1518) 0.001 Neutrophils, % 58.5 (9.2) 57.3 (8.3) 57.9 (8.8) 0.068 Eosinophils, cells/µl 192 (125) 160 (114) 175 (120) 0.0003 Eosinophils < 100 cell/μL 67 (20.6%) 131 (32.8%) 198 (27.3%) 0.0002 Eosinophils > 300 cells/μL 52 (16.0%) 46 (11.5%) 98 (13.5%) 0.080 Eosinophils, % 2.8 (1.8) 2.4 (1.6) 2.6 (1.7) 0.008 Platelets, cells/µl 233 (58) 241 (62) 238 (61) 0.070 Fibrinogen (g/L) 3.9 (0.9) 3.8 (0.9) 3.8 (0.9) 0.208 CRP (mg/dL) 2.0 (4.1) 1.5 (2.8) 1.7 (3.4) 0.0514 Imaging Emphysema, % 10.0 (11.9) 4.7 (7.5) 7.0 (10.0) < 0.0001 Airway wall area, % (Primary bronchi) 18.4 (2.9) 16.9 (3.4) 17.6 (3.3) < 0.0001 Airway wall area, % (Secondary bronchi) 27.5 (5.7) 24.6 (5.6) 25.9 (5.8) < 0.0001 Page 5 of 10 Miravitllesetal. Respiratory Research (2022) 23:49 and independently associated with a higher BEC (Table3). Discussion Given the potential for BEC as a biomarker in COPD [9, 11, 30], it is important to understand the distribution and determinants of BEC in the general adult population and in COPD patients. Our population-based sample of non-COPD adults recruited in the EPISCAN II study had a mean BEC of 160 cells/μL, being significantly higher for COPD patients. We also observed a significantly higher BEC in males, active smokers, and individuals with previous history of asthma and increased levels of eosinophils with increasing severity of airflow obstruction. Since the EPISCAN II was a population-based epidemiological study, it is important to note that the majority of our population of COPD patients had mild disease, with 56% of GOLD stage I, and 75% were undiagnosed and untreated [20]. The mean BEC in non-COPD subjects was 160 cells/ μL, which was significantly lower than the mean count in COPD patients (192 cells/μL). The concentrations observed in our population of non-COPD individuals were intermediate compared to those observed in an Austrian population described by Hartl etal. [17] with a geometric mean of 128 cells/μL, or those reported in a study in the UK (140 cells/μL in smokers and 120 cells/μL in non-smokers) [18], and the concentrations observed in the UK Clinical Practice Research Datalink (182 cells/ μL) [15]. However, they were very similar to the values reported in Copenhagen, with a median value of 150 cells/μL in adult never smokers and 160 cells/μL in ever smokers [16]. Our data replicate previous results showing a significantly higher BEC in subjects with COPD compared with non-COPD controls. In the previously mentioned study by Kolsum etal. [18], the mean BEC was 209 cells/μL in COPD subjects, and in the UK database study, blood eosinophils were a mean of 8% higher in COPD compared with non-COPD subjects [15]. A previous population-based database study in Spain in + 57,000 patients with COPD reported a mean eosinophil count of 253 cells/μL [5], with 28.4% 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% <100 101-200 201-300 >300 percentage COPD Non-COPD Fig. 1 Distribution of BEC in individuals with and without COPD Page 6 of 10 Miravitllesetal. Respiratory Research (2022) 23:49 of individuals with counts > 300 eosinophils/μL, being clearly higher than the 192 cells/μL observed in our study with only 16% with BEC > 300 cells/μL. Similarly, in other Spanish cohorts, Cosio etal. [31] found a mean BEC of 240 cells/μL in a sample of 706 COPD patients without asthma-COPD overlap, and Soler- Cataluña etal. [8] reported a mean BEC of 256 cells/μL in 233 COPD patients, 38.2% with ≥ 300 eosinophils/ μL, while the mean BEC in asthmatic patients was 402 cells/μL. These higher values may be explained by the different characteristics of the participants, since the subjects in these studies had diagnosed COPD with a mean FEV1(%) of between 57 and 64%, and 74% to 85% were men [5, 8, 30] compared with a mean FEV1(%) of 82% and 56% males in our population. Actually, some of these characteristics have been associated with BEC; in our study, male sex, active smoking, previous history of asthma and impaired FEV1(%) were significantly and independently associated with a higher BEC. Moreover, in previous studies, male sex [16, 17], active smoking [17, 18] and asthma diagnosis [15, 17] have been described as being associated with a higher BEC. Other factors associated with increased eosinophils described in large database study are: age < 18years, positive skin prick test, atopy, a positive bronchodilator test, metabolic syndrome and obesity [16, 17]. Our study found not only a significant difference in BEC between COPD and non-COPD individuals, but also an increase in BEC from non-COPD to GOLD stage III/IV COPD, and similarly, a significant negative correlation between eosinophils and FEV1(%) indicating higher levels in more severe disease. Interestingly, we did not observe any significant association between blood eosinophils and exacerbations or ICS treatment, probably due to the characteristics of our population which was mainly made up of mild and untreated patients. Our results concur with those of a previous large database study in patients at low risk of exacerbations in Primary Care that did not observe any influence of treatment with ICS on BEC, and no association was found between blood eosinophils and the frequency of exacerbations [5]. Blood eosinophils were not associated with percent airway wall area for either the central or peripheral airways. Apparently, there was a trend towards a higher BEC in patients with COPD and more emphysema on CT, but the differences were not significant. On the contrary, Papaioannou et al. [32] found that emphysema was associated with low blood eosinophil counts in a group of 98 patients with COPD. Again, these differences may be due to the different characteristics of Table 2 Blood eosinophil levels according to clinical, functional and CT characteristics BMI body mass index, GOLD Global Initiative for Obstructive Lung Disease, COPD chronic obstructive pulmonary disease, YPAS Yale Physical Activity Questionnaire, DLCO diffusion capacity of the lung for carbon monoxide, CI confidence interval, SD standard deviation Blood eosinophil counts P value Mean (SD) (95%CI) n Sex Male 194.8 (127.7) (181.1; 208.5) 336 < 0.0001 Female 158.1 (111.7) (147.0; 169.3) 388 Age 40–50 166.2 (123.4) (139.9; 192.5) 87 0.848 50–60 178.5 (126.8) (161.1; 196.1) 204 60–70 173.0 (118.6) (157.3; 188.8) 219 > 70 177.7 (116.4) (162.0; 193.4) 214 BMI Underweight 166.8 (132.6) (44.2; 289.6) 7 0.928 Normal 171.7 (136.8) (154.4; 189.0) 243 Overweight 178.3 (112.7) (165.6; 191.0) 306 Obese 173.9 (108.5) (157.2; 190.6) 165 Smoking Smoker 189.4 (123.2) (172.2; 206.6) 200 0.025 Former smoker 178.1 (123.3) (164.2; 192.1) 303 Never smoker 158.2 (113.1) (143.2; 173.2) 221 Asthma diagnosis Yes 204.5 (142.0) (171.6; 237.4) 74 0.027 No 171.8 (117.7) (162.8; 180.9) 650 COPD No 160.7 (114.7) (149.4; 172.0) 399 0.001 GOLD I 185.8 (115.9) (168.9; 202.8) 183 GOLD II 197.4 (133.4) (173.3; 221.5) 120 GOLD III/IV 227.0 (158.0) (157.0; 297.1) 22 DLCO > 80% 170.8 (118.5) (160.8; 180.8) 545 0.202 < 80% 184.2 (116.9) (166.2; 202.3) 163 Exacerbations 0 173.5 (119.2) (164.4; 182.5) 667 0.222 1 or more 195.0 (137.1) (158.6; 231.4) 57 YPAS score < 51 sedentary 180.3 (122.1) (168.5; 192.0) 416 0.207 ≥ 51 non‑sedentary 169.2 (118.5) (155.6; 182.7) 295 Emphysema 0–10% 173.0 (123.7) (161.9; 184.1) 480 0.084 > 10% 190.3 (130.5) (167.9; 212.8) 132 Airway Wall thickness (Primary Bronchi) 0–20% 179.9 (124.3) (168.9; 191.0) 485 0.071 > 20% 164.4 (128.4) (141.9; 187.0) 127 Airway Wall thickness (Secondary Bronchi) 0–30% 173.9 (119.5) (163.3; 184.5) 489 0.192 > 30% 190.4 (146.0) (164.2; 216.7) 121 Page 7 of 10 Miravitllesetal. Respiratory Research (2022) 23:49 the populations analysed; they defined emphysema as the presence of emphysema lesions in > 15% of the pulmonary parenchyma, and their emphysema patients had a mean FEV1(%) of 43%. In contrast, there was an underrepresentation of patients with moderate and severe COPD in our sample, and the mean percent emphysema area was 10% in our COPD patients. A recent Canadian study described that patients with COPD and an elevated BEC had thickened central airway walls and a reduction in the total number of visible airways, indicating airway remodelling [33]. More studies are needed to clarify the relationship between BEC and the classic phenotypes of chronic bronchitis and emphysema in COPD. Our study has several limitations, since it was a population-based study of the general population, patients identified with COPD were predominantly mild, undiagnosed and paucisymptomatic, with underrepresentation of patients with other degrees of severity. However, despite these characteristics, there were clear and significant differences in their characteristics compared with non-COPD individuals, including a significantly higher concentration of blood eosinophils. The cross-sectional design did not allow evaluation of the possible prognostic value of BEC for outcomes such as exacerbations. However, the design of the study had the strength to allow the analysis of an unselected population of COPD and non- COPD individuals from all geographic areas of Spain. Finally, we did not systematically assess the presence of possible causes of hypereosinophilia, although they have a low prevalence in the general population. Conclusions The results of our population-based study provide an estimate of the distribution of eosinophils in the healthy adult population in Spain and has demonstrated that COPD patients, even with milder stages, have a significantly higher BEC. In addition to COPD, male sex, active 100 150 200 250 300 350 Male Female Smoker Former smoker Never smoker Yes No No GOLD I GOLD II GOLD III/IV Sex SmokingAsthmaCOPD p=0.0257 p<0.0001 p=0.0274 p=0.0015 Fig. 2 Mean and 95% confidence intervals of BEC of individuals included in the study according to different characteristics. Vertical dotted line denotes mean blood eosinophil value for the whole population Page 8 of 10 Miravitllesetal. Respiratory Research (2022) 23:49 smoking, the presence of asthma and a worse FEV1(%) are significantly associated with a higher BEC. Abbreviations ATS: American Thoracic Society; BEC: Blood eosinophil count; CAT : COPD assessment test; COPD: Chronic obstructive pulmonary disease; CT: Computed tomography; CRP: C‑reactive protein; DLCO: Diffusing capacity of the lung for carbon monoxide; EPISCAN: Epidemiological study of COPD in Spain; ERS: European Respiratory Society; FEV1: Forced expiratory volume in 1 s; FVC: Forced vital capacity; GOLD: Global initiative for obstructive lung disease; ICS: Inhaled corticosteroids; SAS: Statistical analysis system; SD: Standard deviation; 6MWT: Six‑minute walking test; UK: United Kingdom; YPAD: Yale Physical Activ‑ ity Questionnaire. Acknowledgements The collaboration of Monica Sarmiento and Neus Canal of IQVIA, and Carolina Peña and José Julio Jiménez from GSK is explicitly acknowledged. The fol‑ lowing investigators have participated in EPI SCAN II: Scientific Committee: Inmaculada Alfageme, Pilar de Lucas, Julio Ancochea, Marc Miravitlles, Juan José Soler‑Cataluña, Francisco García‑Río, Ciro Casanova, José Miguel Rod‑ ríguez González‑Moro, Borja G. Cosio, Guadalupe Sánchez, and Joan B Soriano. Principal Investigators, collaborators and participating centres: MADRID: H. La Princesa, Julio Ancochea Bermudez (IP) / Elena García Castillo / Claudia Valenzuela / Joan B Soriano. CASTILLA LEÓN: H. U. de Burgos, Ana Pueyo Bastida (IP) / Lourdes Lázaro Asegurado / Luis Rodríguez Pascual / Mª José Mora. ARAGÓN: H. Gral. San Jorge, Luis Borderias Clau (IP) / Lourdes Arizón Mendoza / Sandra García. EXTREMADURA: H. San Pedro de Alcántara, Juan Antonio Riesco Miranda (IP) / Julián Grande Gutiérrez / Jesús Agustín Manzano / Manuel Agustín Sojo González. CASTILLA LEÓN: H. Clínico U. de Salamanca, Miguel Barrueco Ferrero (IP) / Milagros Rosales. GALICIA: H. Álvaro Cunqueiro, José Alberto Fernández Villar (IP) / Cristina Represas / Ana Priegue / Isabel Portela Ferreño / Cecilia Mouronte Roibás / Sara Fernández García. I. BALEARES: H. Son Espases, Borja G Cosío (IP) / Rocío Cordova Díaz / Nuria Toledo Pons / Margalida Llabrés. ARAGÓN: H. U. Miguel Servet, José María Marín Trigo (IP) / Marta Forner / Begoña Gallego / Pablo Cubero / Elisabet Vera. C. VALENCIANA: H. Arnau de Vilanova (Valencia), Juan José Soler Cataluña (IP) / Mª Begoña Picurelli Albero / Noelia González García. ANDALUCÍA: H. Virgen de la Macarena, Agustín Valido Morales (IP) / Carolina Panadero / Cristina Benito Bernáldez/ Laura Martín -Bejarano y Maria Velar. MURCIA: H. Gral. U. Santa Lucía (Cartagena), Antonio Santa Cruz Siminiani (IP) / Carlos Castillo Table 3 Multivariate analysis of the factors associated with the BEC BMI body mass index, CAT COPD assessment test, FEV1 forced expiratory volume in the first second, FVC forced vital capacity, DLCO diffusion capacity of the lung for carbon monoxide, 6MWD 6-min walk distance, YPAS Yale Physical Activity Questionnaire, CRP C-reactive protein. Values in bold are statistically significant. R-Square = 0.067 Parameter Univariate Multivariate P value Estimate (standard error) P value Intercept 208.09 (31.01) < 0.0001 Age (years) 0.4555 Sex Female Reference Reference Male < 0.0001 30.98 (10.65) 0.0038 BMI (kg/m2) 0.8615 Smoking Never smoker Reference Reference Former smoker 0.0620 10.97 (12.73) 0.3892 Active smoker 0.0081 31.21 (13.68) 0.0229 Asthma No Reference Reference Yes 0.0274 36.91 (16.83) 0.0288 CAT score 0.1159 FEV1 (%) 0.0002 − 0.53 (0.28) 0.0543 DLCO (%) 0.0474 YPAS score 0.0493 6 MWD, m 0.3057 Exacerbations last year 0.0331 COPD No Reference Yes 0.0003 Treatment with ICS 0.0099 Platelets, cells/µl 0.1026 Fibrinogen (g/L) 0.9357 CRP (mg/dL) 0.0831 Total emphysema volume (%) 0.0082 0.94 (0.51) 0.0660 % Airway wall area (Primary Bronchi) 0.4094 % Airway wall area (Secondary bronchi) 0.1527 Page 9 of 10 Miravitllesetal. Respiratory Research (2022) 23:49 Quintanilla / Rocío Ibáñez Meléndez / José Javier Martínez Garcerán / Desirée Lozano Vicente / Pedro García Torres / Maria del Mar Valdivia. NAVARRA: Clínica Universidad de Navarra, Juan Pablo de Torres Tajes (IP) / Montserrat Cizur Girones / Carmen Labiano Turrillas. LA RIOJA: H. de San Pedro (Logroño), Carlos Ruiz Martínez (IP)/ Elena Hernando / Elvira Alfaro / José Manuel García / Jorge Lázaro. PAÍS VASCO: H. Santiago Apóstol (H. Txagorritxu), David Bravo (IP) /Laura Hidalgo / Silvia Francisco Terreros / Iñaki Zorrilla / Ainara Alonso Colmenero. ASTURIAS: H. Central de Asturias, Cristina Martínez González (IP) / Susana Margon/ Rosirys Guzman Taveras/ Ramón Fernández / Alicia Álvarez. CANTABRIA: H. de Valdecilla, José Ramón Agüero Balbín (IP) / Juan Agüero Calvo. CATALUÑA: H. U. Vall d’Hebron, Jaume Ferrer Sancho (IP) / Esther Rod‑ ríguez González/ Eduardo Loeb. CASTILLA LA MANCHA: H. U. de Guadalajara, José Luis Izquierdo Alonso (IP) / Mª Antonia Rodríguez García. I. CANARIAS: H. U. de Tenerife, Juan Abreu González (IP)/ Candelaria Martín García/ Rebeca Muñoz/ Haydée Martín García. ASTURIAS: H. U. de San Agustín (Avilés), Miguel Angel Martínez Muñiz (IP) / Andrés Avelino Sánchez Antuña / Jesús Allende González / Jose Antonio Gullón Blanco / Fernando José Alvarez Navascues / Manuel Angel Villanueva Montes / María Rodríguez Pericacho / Concepción Rodríguez García / Juan Diego Alvarez Mavárez. Authors’ contributions The study concept and design: MM, JJS‑C, JBS, FG‑R, PL, IA, CC, JMG‑M, GS, JA, BGC. The acquisition of the data: JJS‑C, JA, BGC. Analysis or interpretation of the data: MM, JJS‑C, JBS, BGC. The drafting of the manuscript: MM. Critical revision and approval for submission: MM, JJS‑C, JBS, FG‑R, PL, IA, CC, JMG‑M, GS, JA, BGC. All authors read and approved the final manuscript. Funding The EPI SCAN II study was funded by an unrestricted grant from GlaxoSmithKline. Availability of data and materials Information on the GSK data sharing commitments and requesting access to anonymized individual participant data and associated documents can be found at www. clini calst udyda tareq uest. com. Declarations Ethics approval and consent to participate The study was approved by the ethics committees of each of the participating centres, and all participants provided informed consent. Consent for publication Not applicable. Competing interests Marc Miravitlles has received speaker or consulting fees from AstraZeneca, Bial, Boehringer Ingelheim, Chiesi, Cipla, CSL Behring, Laboratorios Esteve, Gebro Pharma, Kamada, GlaxoSmithKline, Grifols, Menarini, Mereo Biopharma, Novartis, pH Pharma, Palobiofarma SL, Rovi, TEVA, Spin Therapeutics, Verona Pharma and Zambon, and research grants from Grifols. Juan José Soler‑Cata‑ luña has received speaker fees from AstraZeneca, Bial, Boehringer Ingelheim, Chiesi, Esteve, Ferrer, GlaxoSmithKline, Menarini, Novartis and Teva, and and consulting fees from AstraZeneca, Bial, Boehringer Ingelheim, GlaxoSmith‑ Kline, Ferrer and Novartis. Francisco García‑Río has received speaker or con‑ sulting fees from AstraZeneca, Boehringer Ingelheim, Chiesi, GlaxoSmithKline, Menarini, Novartis, Pfizer and Rovi, and research grants from Chiesi, Esteve, Gebro Pharma, GlaxoSmithKline, Menarini and TEVA. Julio Ancochea has received speaker or consulting fees from Actelion, Air Liquide, Almirall, Astra‑ Zeneca, Boehringer Ingelheim, Carburos Médica, Chiesi, Faes Farma, Ferrer, GlaxoSmithKline, InterMune, Linde Healthcare, Menarini, MSD, Mundipharma, Novartis, Pfizer, Roche, Rovi, Sandoz, Takeda y Teva. Inmaculada Alfageme has no conflicto of interest. Ciro Casanova has received speaker or consulting fees from AstraZeneca, Bial, Boehringer Ingelheim, Chiesi, GlaxoSmithKline, Menarini, Novartis, and research grants from GlaxoSmithKline, Menarini and AstraZeneca. M. Guadalupe Sánches‑Herrero is a GSK employee within the Medical Department. Borja G Cosio has received speaker or consulting fees from AstraZeneca, Boehringer Ingelheim, Chiesi, GlaxoSmithKline, Menarini, Novartis, Sanofi, TEVA, and research grants from Menarini, AstraZeneca and Boehringer‑Ingelheim. Author details 1 Pneumology Department, Hospital Universitari Vall d’Hebron/Vall d’Hebron Institut de Recerca (VHIR), Vall d’Hebron Barcelona Hospital Campus, P. Vall d’Hebron 119‑129, 08035 Barcelona, Spain. 2 CIBER de Enfermedades Respira‑ torias (CIBERES), Instituto de Salud Carlos III (ISCIII), Madrid, Spain. 3 Ser vicio de Neumología, Hospital Arnau de Vilanova‑Lliria, Universidad de la Laguna, Valencia, Spain. 4 Servicio de Neumología, Hospital Universitario la Princesa, Universidad Autónoma de Madrid, Madrid, Spain. 5 Servicio de Neumología, Hospital Universitario la Paz‑IdiPAZ, Madrid, Spain. 6 Servicio de Neumología, Hospital General Gregorio Marañon, Madrid, Spain. 7 Unidad de Gestión Clínica de Neumología, Hospital Universitario Virgen de Valme, Universidad de Sevilla, Sevilla, Spain. 8 Servicio de Neumología, Hospital Universitario Nuestra Señora de Candelaria, Tenerife, Spain. 9 Servicio de Neumología, Hospital Universitario “Príncipe de Asturias”, Alcalá de Henares, Universidad de Alcalá, Madrid, Spain. 10 Departamento Médico, GSK, Tres Cantos, Madrid, Spain. 11 Servicio de Neu‑ mología, Hospital Universitario Son Espases‑IdISBa, Palma de Mallorca, Spain. 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