Assessment of the Effects of Anatoxin-a In Vitro: Cytotoxicity and Uptake
Abstract
Anatoxin-a (ATX-a) is a cyanotoxin whose toxicological profile has been underinvestigated in comparison to other cyanotoxins such as microcystins (MCs) or cylindrospermopsin (CYN). However, its wide distribution, occurrence, and toxic episodes justify more attention. It is classified as a neurotoxin, but it has also been reported to affect other organs and systems. Thus, the aim of this study was to establish, as a first tier in its toxicological evaluation, its cytotoxicity in a wide range of cell lines representative of potential target organs (N2a, SH-SY5Y, HepG2, Caco2, L5178Y Tk+/−, THP-1 and Jurkat). As limited effects were observed after exposure to up to 200 µg/mL of ATX-a for 24 h (only Jurkat and THP-1 cells showed reduced cell viability), cell uptake experiments were performed by ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). The results showed that the immune system cells had the highest percentage of ATX-a in the intracellular fraction, followed by neuronal cells and finally Caco-2 and HepG2 cells. Moreover, the expression of genes related to cell death mechanisms in THP-1 cells was also analyzed by polymerase chain reaction (PCR) and showed no changes under the conditions tested. Further research is required on ATX-a’s toxic effects and toxicokinetics to contribute to its risk assessment.
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Citation: Plata-Calzado, C.; Prieto, A.I.; Cameán, A.M.; Jos, A. Assessment of the Effects of Anatoxin-a In Vitro: Cytotoxicity and Uptake. Toxins 2024,16, 541. https:// doi.org/10.3390/toxins16120541 Received: 25 October 2024 Revised: 2 December 2024 Accepted: 12 December 2024 Published: 13 December 2024 Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Article Assessment of the Effects of Anatoxin-a In Vitro: Cytotoxicity and Uptake Cristina Plata-Calzado , Ana I. Prieto * , Ana M. Cameán and Angeles Jos Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González 2, 41012 Seville, Spain; [email protected] (C.P.-C.); [email protected] (A.M.C.); [email protected] (A.J.) *Correspondence: [email protected] Abstract: Anatoxin-a (ATX-a) is a cyanotoxin whose toxicological profile has been underinvestigated in comparison to other cyanotoxins such as microcystins (MCs) or cylindrospermopsin (CYN). However, its wide distribution, occurrence, and toxic episodes justify more attention. It is classified as a neurotoxin, but it has also been reported to affect other organs and systems. Thus, the aim of this study was to establish, as a first tier in its toxicological evaluation, its cytotoxicity in a wide range of cell lines representative of potential target organs (N2a, SH-SY5Y, HepG2, Caco2, L5178Y Tk +/− , THP-1 and Jurkat). As limited effects were observed after exposure to up to 200 µ g/mL of ATX-a for 24 h (only Jurkat and THP-1 cells showed reduced cell viability), cell uptake experiments were performed by ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLCMS/MS). The results showed that the immune system cells had the highest percentage of ATX-a in the intracellular fraction, followed by neuronal cells and finally Caco-2 and HepG2 cells. Moreover, the expression of genes related to cell death mechanisms in THP-1 cells was also analyzed by polymerase chain reaction (PCR) and showed no changes under the conditions tested. Further research is required on ATX-a’s toxic effects and toxicokinetics to contribute to its risk assessment. Keywords: anatoxin-a; cytotoxicity; in vitro; uptake; gene expression; risk assessment Key Contribution: ATX-a only induced cytotoxicity in THP-1 and Jurkat cells at high concentrations. ATX-a was detected inside the established cell lines at different levels. No relationship between cytotoxicity and toxin uptake was observed. ATX-a did not change apoptosis/necrosis-related gene expression in THP-1 cells. 1. Introduction Cyanobacterial blooms can pose a risk to the environment and public health as a result of their ability to produce cyanotoxins. Factors such as climate change and pollution from anthropogenic activity can lead to an increase in the frequency of such blooms. Therefore, the investigation of the toxic effects produced by cyanotoxins such as microcystins (MCs), cylindrospermopsin (CYN) or anatoxin-a (ATX-a) is a priority [ 1 , 2 ]. Of all these cyanotoxins, ATX-a is the least studied. According to the World Health Organization (WHO), more toxicological data are needed in order to update the provisional reference values established for ATX-a [3]. ATX-a is a secondary amine alkaloid produced by numerous cyanobacteria genders such as Anabaena,Aphanizomenon,Oscillatoria, or Microcystis [4]. ATX-a has been reported in Africa [ 5 , 6 ], Asia [ 7 , 8 ], Europe [ 9 – 12 ], America [ 13 , 14 ] and Oceania [ 15 , 16 ]. Furthermore, its presence is not limited to freshwater, but several studies have reported ATX-a in brackish water [ 17 , 18 ]. In terms of detected concentrations of ATX-a, this cyanotoxin typically remains below 10 µ g/L in open water [ 3 ], although concentrations higher than 1000 µ g/L have been documented in surface water [ 19 ]. Moreover, Wood et al. [ 20 ] detected up to 100-fold variations in the amount of ATX-a in toxic strains isolated from the same mat. These findings reveal that there is a large variability in the ATX-a Toxins 2024,16, 541. https://doi.org/10.3390/toxins16120541 https://www.mdpi.com/journal/toxins
Toxins 2024,16, 541 2 of 16 content in different environmental samples, so toxic effects also need to be investigated in a wide concentration range. Regarding its toxicity, it is important to note that several studies have described animal poisonings, mainly in dogs, due to exposure to this cyanotoxin [ 21 – 25 ]. In humans, the routes of exposure to ATX-a include inhalation, dermal, and oral routes, with the latter being the most relevant due to the consumption of contaminated water or food (mainly fish and vegetables) [ 26 ]. In this regard, human intoxication has recently been described as the result of the ingestion of ATX-a-contaminated sea figs [ 27 ], as well as the presence of ATX-a in bivalve samples at the Ingril site and at the Le Scorésite [ 28 ]. The results of these studies highlight the need to carry out ATX-a toxicity studies to clarify the consequences of the exposure to this cyanotoxin. Early toxicity studies of ATX-a in isolated organs focused on demonstrating the mechanism of action, observing a high isomer-dependent affinity for nicotinic acetylcholine receptors (AChR) [ 29 – 31 ], with the (+)-ATX-a isomer being more than 100 times more potent than the ( − )-ATX-a isomer [ 30 ]. Binding of ATX-a to AChR produces a neuromuscular blockade that results in symptoms such as fatigue, convulsions, paralysis, and respiratory failure, among others [ 3 ]. For this reason, ATX-a is classified mainly as a neurotoxin. Nevertheless, few studies have explored the toxic effects of this cyanotoxin using neuronal cell lines and have shown conflicting results. Thus, Takser et al. [ 32 ] studied the toxic effects of ATX-a on the N2a cell line. This study showed a significant reduction in N2a viability compared to the control group by MTT assay after exposure for 24, 48 and 72 h to 0.1 and 10 µ M ATX-a. However, Kubickova et al. [ 33 ] did not observe ATX-a cytotoxicity in any of the assays performed (neutral red uptake, reduction in resazurin, lactate dehydrogenase release, acetoxymethyl ester cleavage and carboxyfluorescein diacetate) in human neural stem cells derived from the H9 cell line after exposure to concentrations of up to 20 µ M ATX-a for 4 days. In addition, interestingly, the effects of ATX-a on other tissues and systems have been reported. Thus, ATX-a has been shown to exhibit immunotoxic [ 32 , 34 – 37 ], cytotoxic [ 38 , 39 ], genotoxic [ 40 ] or cardiovascular effects [ 41 – 43 ]. However, most of these studies have been carried out on isolated organs or cells or used in vivo models. Overall, although in vitro studies are one of the first steps in the toxicity evaluation of compounds, studies focused on the toxic effects of ATX-a in established cell lines are limited. Therefore, the aim of the present study was to investigate the potential cytotoxicity of ATX-a in different cell lines as the first tier in the process to elucidate its toxicological profile. Specifically, neuronal cell lines (N2a, SH-SY5Y), immune system cell lines (THP-1, Jurkat and L5178Y Tk +/− ), and cell lines representative of an oral exposure (HepG2 and Caco-2) were employed, in which the possible cytotoxic effects of this cyanotoxin have been scarcely or not yet explored. Furthermore, in order to correctly interpret the results obtained in cell viability assays, the ATX-a uptake by the seven cell lines used was also analyzed, and for comparative purposes, CYN cytotoxicity and uptake in THP-1 cells were also included. Finally, the effects on the expression of genes related to apoptosis and necrosis mechanisms were explored in the cell line in which the highest cytotoxic effect of ATX-a was observed (THP-1 cell line). 2. Results 2.1. Cytotoxicity Assays The results obtained in the cytotoxicity assays with ATX-a are shown in Figure 1. A non-significant reduction in viability was observed in neuronal cells (SH-SY5Y and N2a) after 24 h of exposure to 0–200 µ g/mL of ATX-a fumarate (equivalent to 0–117.5 µ g/mL pure ATX-a) (Figure 1a). Similar results were obtained in the Caco-2 and HepG2 cell lines after the same exposure conditions (Figure 1b). A decrease in viability of the Jurkat cell line was observed after exposure to ATX-a fumarate for 24 h at all tested concentrations (Figure 1c). However, a significant decrease in cell viability was only obtained in the THP-1
Toxins 2024,16, 541 3 of 16 cell line after exposure to the maximum concentration tested (200 µ g/mL) compared to the control group. Toxins2024,16,xFORPEERREVIEW3of17 linewasobservedafterexposuretoATX-afumaratefor24hatalltestedconcentrations (Figure1c).However,asignificantdecreaseincellviabilitywasonlyobtainedintheTHP- 1celllineafterexposuretothemaximumconcentrationtested(200µg/mL)comparedto thecontrolgroup. Figure1.ReductionintetrazoliumsaltMTSin(a)neuronalcells(SH-SY5YandN2a),(b) Caco-2andHepG2cellsand(c)immunesystemcells(THP-1,JurkatandL5178YTk+/−) after24hofexposureto0–200µg/mLATX-afumarate.Inaddition,0.3%v/vTritonX- 100wasusedasthepositivecontrol.Allexperimentswereperformedatleastintriplicateperconcentration.Dataareexpressedasmean±SDcomparedtonegativecontrol group.Note:*p<0.05and***p<0.001indicatedsignificantdifferencefromnegative control. AsforCYN,areductionincellviabilitywasobservedatallconcentrationsemployed. Specifically,anEC20wasobtainedinTHP-1cellsafterexposureto1.1µg/mLoftoxinfor 24h.Theotherconcentrationsassayed(2.5and3µg/mLCYN)showedaviabilityofTHP- 1cells69.05±8.7%and57.67±3.5%,respectively,comparedtothenegativecontrol(Figure 2). Figure 1. Reduction in tetrazolium salt MTS in (a) neuronal cells (SH-SY5Y and N2a), (b) Caco-2 and HepG2 cells and (c) immune system cells (THP-1, Jurkat and L5178Y Tk +/− ) after 24 h of exposure to 0–200 µ g/mL ATX-a fumarate. In addition, 0.3% v/vTriton X-100 was used as the positive control. All experiments were performed at least in triplicate per concentration. Data are expressed as mean ±SD compared to negative control group. Note: * p< 0.05 and *** p< 0.001 indicated significant difference from negative control. As for CYN, a reduction in cell viability was observed at all concentrations employed. Specifically, an EC20 was obtained in THP-1 cells after exposure to 1.1 µ g/mL of toxin for 24 h. The other concentrations assayed (2.5 and 3 µ g/mL CYN) showed a viability of THP-1 cells 69.05 ± 8.7% and 57.67 ± 3.5%, respectively, compared to the negative control (Figure 2). Toxins2024,16,xFORPEERREVIEW4of17 Figure2.ReductionintetrazoliumsaltMTSinTHP-1cellsexposedto0–3µg/mLCYN for24h.Inaddition,0.3%v/vTritonX-100wasusedasthepositivecontrol.Experiments wereperformedintriplicateperconcentration.Dataareexpressedasmean±SDcomparedtonegativecontrolgroup.Note:*p<0.05and***p<0.001indicatesignificantdifferencefromthenegativecontrol. 2.2.UptakeofATX-a AnanalysisofATX-auptakebythecelllinesusedinthecytotoxicityassayswasperformedbyUHPLC-MS/MS.Thelimitsofdetection(LOD)andquantification(LOQ)ofthe analyticalmethodwere1.88and6.28µg/L,respectively.Asanexample,ATX-auptake chromatogramsintheTHP-1celllineafterexposureto50µg/mLATX-afumaratefor24 hareshown(Figure3).Chromatogramscorrespondingtotheothersixcelllinesexposed tothesameconcentrationofATX-afumarateareavailableintheSupplementaryMaterial (FiguresS1–S3). Figure 2. Reduction in tetrazolium salt MTS in THP-1 cells exposed to 0–3 µ g/mL CYN for 24 h. In addition, 0.3% v/vTriton X-100 was used as the positive control. Experiments were performed in triplicate per concentration. Data are expressed as mean ± SD compared to negative control group. Note: * p< 0.05 and *** p< 0.001 indicate significant difference from the negative control.
Toxins 2024,16, 541 4 of 16 2.2. Uptake of ATX-a An analysis of ATX-a uptake by the cell lines used in the cytotoxicity assays was performed by UHPLC-MS/MS. The limits of detection (LOD) and quantification (LOQ) of the analytical method were 1.88 and 6.28 µ g/L, respectively. As an example, ATX-a uptake chromatograms in the THP-1 cell line after exposure to 50 µ g/mL ATX-a fumarate for 24 h are shown (Figure 3). Chromatograms corresponding to the other six cell lines exposed to the same concentration of ATX-a fumarate are available in the Supplementary Material (Figures S1–S3). Toxins2024,16,xFORPEERREVIEW5of17 Figure3.ChromatogramsobtainedbyUHPLC-MS/MSofATX-ainTHP-1cellsexposedto50µg/mL ATX-afumaratefor24h.(a)Negativecontrol,(b)intracellularfractionand(c)extracellularfraction. TheresultsobtainedinthedeterminationofATX-ainthecelllinesassayedareshown inTable1.Inthisway,takingintoaccounttheamountoftoxinadded,between43.55and 129.84%ofATX-awasdetectedintheanalysis.TheresultsshowedATX-aintheintracellularfractionofallcellsanalyzed.However,theamountsdetectedineachofthecelltypes weredifferent.Specifically,ahigheruptakewasobservedinthefollowingcelllinesbelongingtotheimmunesystem:L5178YTk+/−(6.45–9.70%),Jurkat(10.58–13.96%)andTHP- 1(6.62–14.72%)oftotalATX-aadded,followedbyneuronalcelllines(between1.52and 2.94%)andfinally,inCaco-2andHepG2(between0.30and0.65%oftotaltoxinadded) (Table1). (a) (b) (c) Figure 3. Chromatograms obtained by UHPLC-MS/MS of ATX-a in THP-1 cells exposed to 50 µ g/mL ATX-a fumarate for 24 h. (a) Negative control, (b) intracellular fraction and (c) extracellular fraction. The results obtained in the determination of ATX-a in the cell lines assayed are shown in Table 1. In this way, taking into account the amount of toxin added, between 43.55
Toxins 2024,16, 541 5 of 16 and 129.84% of ATX-a was detected in the analysis. The results showed ATX-a in the intracellular fraction of all cells analyzed. However, the amounts detected in each of the cell types were different. Specifically, a higher uptake was observed in the following cell lines belonging to the immune system: L5178Y Tk +/− (6.45–9.70%), Jurkat (10.58–13.96%) and THP-1 (6.62–14.72%) of total ATX-a added, followed by neuronal cell lines (between 1.52 and 2.94%) and finally, in Caco-2 and HepG2 (between 0.30 and 0.65% of total toxin added) (Table 1). Table 1. Mean ± SD (%) of intracellular and extracellular ATX-a in N2a, SH-SY5Y, L5178YTk +/− , Jurkat, THP-1, HepG2, and Caco-2 cell lines after exposure to 50 or 100 µ g/mL ATX-a fumarate for a period of 24 h by triplicate. n.d.: not detected. ATX-a Fumarate (µg/mL) % Intracellular Mean ±SD % Extracellular Mean ±SD % Total ATX-a Detected Mean ±SD N2a C−n.d. n.d. n.d. 50 µg/mL 1.71 ±1.15 59.90 ±4.32 61.61 ±3.59 100 µg/mL 1.52 ±0.66 42.03 ±11.73 43.55 ±11.36 SH-SY5Y C−n.d. n.d. n.d. 50 µg/mL 1.99 ±0.67 127.85 ±8.71 129.84 ±9.38 100 µg/mL 2.94 ±1.91 106.91 ±39.49 109.85 ±40.62 L5178YTk+/− C−n.d. n.d. n.d. 50 µg/mL 9.70 ±4.78 72.61 ±9.54 82.31 ±10.34 100 µg/mL 6.45 ±1.31 69.44 ±10.50 75.89 ±9.45 Jurkat C−n.d. n.d. n.d. 50 µg/mL 10.58 ±0.74 75.21 ±23.23 85.78 ±23.85 100 µg/mL 13.96 ±3.48 74.14 ±15.58 88.11 ±15.99 THP-1 C−n.d. n.d. n.d. 50 µg/mL 14.72 ±4.55 93.90 ±3.35 108.62 ±7.46 100 µg/mL 6.62 ±1.37 56.35 ±5.35 62.98 ±4.80 HepG2 C−n.d. n.d. n.d. 50 µg/mL 0.65 ±0.05 104.03 ±2.82 104.72 ±2.87 100 µg/mL 0.30 ±0.02 62.52 ±4.87 62.81 ±4.89 Caco-2 C−n.d. n.d. n.d. 50 µg/mL 0.46 ±0.15 83.72 ±30.85 84.18 ±30.75 100 µg/mL 0.52 ±0.22 72.02 ±21.88 72.54 ±21.88 2.3. Uptake CYN The results of CYN uptake in the THP-1 cell line expressed as % of CYN detected with respect to the total amount of toxin added are shown in Table 2. The percentages of detected CYN ranged from 57.81 to 65.85%. An increase in the percentage of intracellular CYN was observed as the concentration of exposure to this cyanotoxin increased. Table 2. Mean ± SD (%) of intracellular and extracellular CYN in THP-1 cells after exposure to 0. 1.1, 2.5 and 3 µg/mL CYN for a period of 24 h by triplicate. n.d.: not detected. CYN (µg/mL) % Intracellular Mean ±SD % Extracellular Mean ±SD % Total CYN Detected Mean ±SD THP-1 C−n.d. n.d. n.d. 1.1 µg/mL 0.38 ±0.05 59.74 ±1.01 60.12 ±1.06 2.5 µg/mL 0.71 ±0.16 57.10 ±2.19 57.81 ±2.21 3µg/mL 1.30 ±0.58 64.56 ±4.29 65.85 ±4.83
Toxins 2024,16, 541 6 of 16 2.4. Gene Expression Analysis by Quantitative Real-Time PCR The effects of ATX-a exposure for 24 h on mRNA expression of genes involved in cell death mechanisms were analyzed in the THP-1 cell line by RT-qPCR (Figure 4). ATX-a did not produce changes in the expression of the genes investigated at either of the two concentrations assayed (5 and 50 µ g/mL ATX-a fumarate) under the conditions tested. Changes in gene expression were only observed after exposure to the positive control: upregulation in BAX and downregulation in BCL2. Toxins2024,16,xFORPEERREVIEW7of17 Figure4.EffectsofATX-aontheexpressionofmRNAofgenesinvolvedinapoptosis/necrosisin THP-1cells.Cellswereexposedto5or50µg/mLATX-afumaratefor24h.CPT(0.5µM)wasused aspositivecontrolandchloroform/methanol(4:1)assolventcontrol.ResultsareexpressedasrelativemRNAexpressionnormalizedtothenegativecontrolgroup.*p<0.05and****p<0.0001indicatesignificantlydifferencefromnegativecontrols. 3.Discussion AlthoughATX-aisawell-knowncyanotoxin,theamountofstudiesandexperimental datadealingwithitislowercomparedtoothercyanotoxinssuchasmicrocystins[44]or cylindrospermopsin[45],asitwasevidencedbythereviewperformedbyPlata-Calzado etal.[46].However,itisawidelyreportedtoxininimportantcasesofdeathsofdogs, livestockandwildanimalspoisoning,withconvulsions,ataxiaandparesthesiaofcerebral hypoxiabeingsomeofthemaintoxiceffectsobservedafterATX-aexposure[3].Inaddition,theWHOpointedoutthatthetoxicologicaldatabaseonATXswasnotadequateto supportderivationofaformalguidelinevalue[3].Thus,tofurtherexplorethemechanismsofATX-atoxicity,cellviabilitystudieswithdifferentestablishedcelllineswereperformed.Theseassaysareabasicapproachintoxicity-testingstrategiesandcouldsupport theestablishmentoftheadverseoutcomepathways(AOPs)ofthestudiedchemical.As ATX-aisprimarilyclassifiedasaneurotoxin,twodifferentneuronalcells,SHSY-5Yand N2a,werefirstselected.BothhavebeenreportedtoexpressAChR,ofwhichATX-aisan agonist[47,48].Surprisingly,noevidentcytotoxicitywasobservedatlowconcentrations onSH-SY5Y,butevenathighconcentrations(upto200µg/mL)inbothcelllines.Onthe contrary,CYN,althoughnotspecificallyclassifiedasaneurotoxin,wasreportedtoinduce ahighercytotoxicityonSHSY-5Ycultures,withanEC50-24hvalueofonly0.87±0.13 µg/mLusingthesameassay[49].ThenumberofstudiesofATX-ainneuronalstablished celllinesislimited.OnlyTakseretal.[32]reportedcytotoxicityinN2acellsafterexposure to0.1and10µMATX-a(equivalentto≈0.028and2.8µg/mL,respectively)intheMTT assay,althoughtheeffectswereofsimilarmagnitude,withoutaconcentration-dependent pattern.Inaddition,anEC50wasonlyreachedafter72hofexposuretoATX-a.Incontrast, theseATX-aconcentrationsdidnotinducecytotoxicityinBV-2cells(murinemicroglial cells). Ascyanotoxinsarereportedtoinducetoxicityatdifferentlevels,otherestablished celllinesofadditionaltargetorganswereselectedtotestATX-ainordertocheckcytotoxicityresponses.Thus,HepG2andCaco-2cellswereusedtakingintoaccountthatoral exposureisthemainexposureforcyanotoxinsinthegeneralpopulation.Butresultsprovidedasimilaroutcome,noevidentcytotoxicity.Toourknowledge,nostudiesonthe possibleinvitroadverseeffectsofATX-aonthegastrointestinalsystemhavebeenpublished,althoughalterationsinenzymebiomarkershavebeendetectedinfishliverafter intraperitonealadministrationofATX-a[50]. Figure 4. Effects of ATX-a on the expression of mRNA of genes involved in apoptosis/necrosis in THP-1 cells. Cells were exposed to 5 or 50 µ g/mL ATX-a fumarate for 24 h. CPT (0.5 µ M) was used as positive control and chloroform/methanol (4:1) as solvent control. Results are expressed as relative mRNA expression normalized to the negative control group. * p< 0.05 and **** p< 0.0001 indicate significantly difference from negative controls. 3. Discussion Although ATX-a is a well-known cyanotoxin, the amount of studies and experimental data dealing with it is lower compared to other cyanotoxins such as microcystins [ 44 ] or cylindrospermopsin [ 45 ], as it was evidenced by the review performed by Plata-Calzado et al. [ 46 ]. However, it is a widely reported toxin in important cases of deaths of dogs, livestock and wild animals poisoning, with convulsions, ataxia and paresthesia of cerebral hypoxia being some of the main toxic effects observed after ATX-a exposure [ 3 ]. In addition, the WHO pointed out that the toxicological database on ATXs was not adequate to support derivation of a formal guideline value [ 3 ]. Thus, to further explore the mechanisms of ATX-a toxicity, cell viability studies with different established cell lines were performed. These assays are a basic approach in toxicity-testing strategies and could support the establishment of the adverse outcome pathways (AOPs) of the studied chemical. As ATX-a is primarily classified as a neurotoxin, two different neuronal cells, SHSY-5Y and N2a, were first selected. Both have been reported to express AChR, of which ATX-a is an agonist [ 47 , 48 ]. Surprisingly, no evident cytotoxicity was observed at low concentrations on SH-SY5Y, but even at high concentrations (up to 200 µ g/mL) in both cell lines. On the contrary, CYN, although not specifically classified as a neurotoxin, was reported to induce a higher cytotoxicity on SHSY-5Y cultures, with an EC 50 -24h value of only 0.87 ± 0.13 µ g/mL using the same assay [ 49 ]. The number of studies of ATX-a in neuronal stablished cell lines is limited. Only Takser et al. [ 32 ] reported cytotoxicity in N2a cells after exposure to 0.1 and 10 µ M ATX-a (equivalent to ≈ 0.028 and 2.8 µ g/mL, respectively) in the MTT assay, although the effects were of similar magnitude, without a concentration-dependent pattern. In addition, an EC 50 was only reached after 72 h of exposure to ATX-a. In contrast, these ATX-a concentrations did not induce cytotoxicity in BV-2 cells (murine microglial cells). As cyanotoxins are reported to induce toxicity at different levels, other established cell lines of additional target organs were selected to test ATX-a in order to check cytotoxicity responses. Thus, HepG2 and Caco-2 cells were used taking into account that oral exposure is
Toxins 2024,16, 541 7 of 16 the main exposure for cyanotoxins in the general population. But results provided a similar outcome, no evident cytotoxicity. To our knowledge, no studies on the possible in vitro adverse effects of ATX-a on the gastrointestinal system have been published, although alterations in enzyme biomarkers have been detected in fish liver after intraperitoneal administration of ATX-a [50]. Additionally, cells lines related to the immune system (monocytes and lymphoblasts) were selected. In this case, L5178Y Tk +/− cells did not experience toxic effects, Jurkat cells reduced its viability in a non-significant way, and THP-1 cells significantly reduced its viability at the highest concentration tested (200 µ g/mL). Scientific literature reports toxic effects of ATX-a on the immune system. Thus, Takser et al. [ 32 ] observed cytotoxicity in the RAW246.7 murine macrophage-like cell line exposed to 0.1 and 10 µ M ATX-a. Moreover, ATX-a has shown its toxicity on immunological models from fish [ 36 , 37 ] but using isolated cells and not established cell lines, making the comparison more difficult. Regarding mammalian models, apart from Takser et al. [ 32 ], the most relevant studies to compare with would be those by Rao et al. [ 34 ] and Teneva et al. [ 35 ]. Rao et al. [ 34 ] exposed thymocytes isolated from 4- to 8-week-old rats to (+)-ATX-a and observed a strong concentration-dependent decrease in cell viability with a total reduction at 50 µ g/mL. Teneva et al. [ 35 ] also observed a significant cytotoxicity in spleen cells isolated from male 8-week-old BALB/c mice exposed to 0.1 µ g/mL for 24 h, with no specification of the isomer used. These differences observed with respect to our study could be due to the ATX-a isomer used. Thus, Zhang et al. [ 30 ] found that (+)-ATX-a was 160-fold more potent than ( − )-ATX-a, and 20-fold more potent than ( ± )-ATX-a in inhibiting [ 3 H]ACh binding in rat brains. However, Molloy et al. [ 31 ] used synthetic ( ± )-ATX-a (up to 100 µ g/mL) in bovine adrenal chromaffin cells showing that ATX-a was a potent nicotinic acetylcholine receptor agonist in these cells. Similarly, Liu et al. [ 51 ] also used the racemic mixture, and they reported agonist activity in the yeast oestrogen screen assay in Saccharomyces cerevisiae. In our study, the concentrations of (+)-ATX-a contained in the racemic mixture exceed those used by Rao et al. [ 34 ] and Teneva et al. [ 35 ], so the experimental model (primary culture versus established cell line) could be the main reason for the differences found with respect to our results. Thus, considering the cytotoxicity data obtained from a general point of view, it has been evidenced that ATX-a showed a limited cytotoxicity in the established cell lines tested, and that the type of experimental model used could play a role. However, Takser et al. [ 32 ] used the same cell line (N2a), a similar assay (MTT assay, based also on the mitochondrial enzyme succinate dehydrogenase activity), and the same toxin (ATX-a from Enzo) and they observed cytotoxicity. More recently, Kubickova et al. [ 33 ] have tested ATX-a using a differentiating in vitro human neural stem cell model and observed that ATX-a (up to 20 µM ) did not cause distinct developmental neurotoxicity alone, and that its (excito-)toxicity to acetylcholinergic neurons occurred only in co-exposure to all-trans retinoic acid. The results could also be explained due to the non-effective uptake of ATX-a by the cells. Thus, other widely studied cyanotoxins, such as MCs [ 2 ], are known to require specific transporters to enter the cells. They are uptaken by the organic acid transporter polypeptides (OATPs) and limited toxicity on established cell lines has been explained by the lack of these specific transporters [ 52 ]. Unfortunately, data regarding ATX-a kinetics are limited, and no reports suggest the participation of transporters. Thus, Testai [ 2 ] stated that ATX-a is passively and rapidly absorbed after ingestion and widely disseminated to different tissues, including the brain. Thus, to check the uptake of ATX-a by the cells, the determination of the toxin was performed in the supernatants and inside the cells after 24 h of exposure to the toxin. The results showed that the uptake in all cell lines was limited. Cells of immunological nature showed a higher intracellular presence than those from the nervous system and from the gastrointestinal system. This was in agreement with the cytotoxicity results. Also, an interesting observation derived from the data obtained is that the uptake (intracellular recovery) was concentration-dependent in all cell lines, with the
Toxins 2024,16, 541 8 of 16 exception of the THP-1 and HepG2 cell lines, where the amount of toxin detected in the intracellular fraction was similar at both concentrations. Therefore, at least a small part of ATX-a could be taken up by passive diffusion, which would explain why it is detected inside all cell lines and that the amount was concentration-dependent. In addition, the main aim of the present work was to analyze whether the content of uptaken toxin was relevant to induce cytotoxicity. However, the results do not seem to show a relationship between uptake and the observed toxic effects, and with that aim, a similar experiment with CYN was performed for comparative purposes. Thus, cytotoxicity-causing CYN concentrations were used, and the percentage of cytotoxicity and uptake was analyzed. The cytotoxicity assays performed by Casas-Rodriguez et al. [ 53 ] were replicated in the THP-1 cell line, obtaining similar results (EC 20 = 1.1 µ g/mL and approximately EC 50 at a concentration of 3µg/mL CYN). Regarding toxin uptake, a concentration-dependent increase in intracellular % was also observed. However, the % intracellular recovery was very low and even much lower than in the case of ATX-a, and despite this, an evident cytotoxicity was observed. Therefore, the absence of ATX-a cytotoxicity is not only a consequence of low toxin uptake. A different hypothesis to try to explain the absence of effects is that cytotoxicity is a non-very sensitive biomarker of cell damage, and very high concentrations are required. This argument was used by Menezes et al. [ 54 ] for the toxic effects induced by MC-LR in the kidney Vero-E6 cell line. They concluded that the type and extent of effects were largely influenced by the toxin concentration, noting that certain responses, like cell death by apoptosis or necrosis, need much higher concentration compared to others, such as the expression of mitogen-activated protein kinase (MAPK). In order to test this hypothesis, the expression of genes involved in cell death was analyzed in the cell line most sensitive to cytotoxic effects, THP-1 cells. Also, it is known that for other cyanotoxins, such as CYN, low concentrations (EC 20 values) can already induce important changes at a genetic level [ 53 ]. However, in this case, again, no effects were observed. Nevertheless, we cannot dismiss the possibility that the lack of direct cytotoxicity of ATX-a could imply a potential ATX-a toxicity mediated by other responses such as interactions with certain molecular receptors or altering cellular processes that do not result in immediate cell death. On the other hand, in comparison to CYN, it might happen that the limited in vitro cytotoxicity observed for ATX-a is just a matter of toxic potency. Thus, CYN has proved to be more cytotoxic than ATX-a on the different cell lines studied [ 49 , 53 , 55 , 56 ]. Regarding in vivo data, median lethal dose values after acute exposure reported by the WHO [ 3 , 57 ] were lower for CYN than for ATX-a, but a direct comparison is not possible due to differences in exposure, purity and concentrations of toxin used, etc. Overall, the results obtained in this research reveal important issues regarding the selection of the most suitable experimental models and biomarkers to use in ATX-a toxicological research. However, the observation that ATX-a is taken up by all cell lines tested suggests that this toxin can potentially affect various tissue types and that there is no cytotoxic potential in these cell models under the conditions tested. In view of the results obtained, further research with more advanced and sensitive experimental models is needed to elucidate their possible absorption pathways and toxicity mechanisms, in order to clarify their toxic potential to support an adequate risk assessment. 4. Conclusions The cytotoxicity of ATX-a in the cell lines evaluated was low even at high concentrations (200 µ g/mL). This may be due to different reasons among which the experimental model, isomer specificity of toxin, toxin uptake or the cell damage marker used stand out. The ATX-a uptake results showed toxin inside all the cell lines studied, concluding that the absence of cytotoxicity was not due to a limited uptake. In contrast, CYN with a lower uptake induced higher cytotoxicity. Also, no response was observed in the expression of genes related to the apoptosis and necrosis response. The results seem to indicate that the established cell lines might not be a suitable model to study ATX-a toxicity, and that ATX-a
Toxins 2024,16, 541 9 of 16 toxic potency is lower than for CYN. Thus, although the established cell lines are a first step in the investigation of the toxic effects of substances, the present study shows that in the case of ATX-a, common cell models such as N2a, SH-SY5Y, THP-1, Jurkat, L5178Y Tk +/− , HepG2 and Caco-2 cell lines were not the most appropriate. Studies with more advanced models such as primary cell cultures or in vivo systems are needed to better understand the mechanisms and toxic effects of ATX-a. 5. Materials and Methods 5.1. Chemicals ( ± ) Anatoxin-a fumarate standard with a purity greater than 98.0% and cylindrospermopsin with a purity of 95% were provided by Enzo Life Sciences (Lausen, Switzerland). The reagents needed for cell culture were sourced from Gibco (Biomol, Sevilla, Spain). Reagents for cyanotoxin extraction and LC-MS/MS analysis were obtained from Sigma– Aldrich (Madrid, Spain). Finally, the chemical reagents used for RT-qPCR were provided by Qiagen (Madrid, Spain) and Bio-Rad Laboratories (Hercules, CA, USA). 5.2. Cell Lines and Culture Conditions The cell lines as well as the culture conditions used are detailed in Table 3. The recommendations of the supplier of the cell lines (the American Type Culture Collection (ATCC)) for their maintenance were followed. Table 3. Cell lines employed in the study and culture conditions for the maintenance. Cell Line Cell Type Tissue Type Model Organism Culture Media Culture Conditions N2a Neuroblast Brain Mouse 43.5% OptiMEM + 43.5% DMEM high glucose + 10% FBS, 1% piruvate 1% L-glutamine and 1% penicillin–streptomycin Incubator at 37 ◦C with 95% relative humidity and 5% CO2 SH-SY5Y Neuroblast Brain Human 43% MEM + 43% Ham’s F12 + 10% FBS, 1% piruvate 1% L-glutamine, 1% NEAA and 1% penicillin–streptomycin Jurkat T lymphoblast Peripheral blood Human RPMI 1640 medium containing high glucose (R8005, Sigma Aldrich) + 10% FBS, 1% penicillin–streptomycin and 2g/L sodium bicarbonate THP-1 Monocyte Peripheral blood Human L5178Y Tk+/−Lymphoblast Lymph node Mouse RPMI 1640 medium + 10% horse serum, 1% pyruvate, 1% L-glutamine and 1% penicillin–streptomycin Caco-2 Epithelial cell Colon Human MEM medium + 10% FBS, 1% NEAA, 1% pyruvate, 1% L-glutamine and 1% penicillin–streptomycin HepG2 Hepatocyte Liver Human MEM medium + 10% FBS, 1% L-glutamine and 1% penicillin–streptomycin DMEM: Dulbecco’s modified eagle; MEM: minimum essential medium FBS: fetal bovine serum; RPMI: Roswell Park Memorial Institute; NEAA: non-essential amino acids. 5.3. Test Solutions Stock solutions of ATX-a fumarate (1000 µ g/mL) and CYN (1000 µ g/mL) were prepared in milli-Q sterile water and maintained at − 20 ◦ C. Toxin concentrations were prepared in the corresponding cell culture medium.
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