Deep Eutectic Solvents as Catalysts in the Synthesis of Active Pharmaceutical Ingredients and Precursors
Abstract
Deep Eutectic Solvents (DESs) have appeared in recent years as an appealing alternative to classical organic solvents, due to their valuable environmental properties. In addition, these compounds, formed by the combination of one hydrogen bond donor with a hydrogen bond acceptor at a defined stoichiometric ratio, present other valuable activities not only as a reaction medium. DESs can also be employed as catalysts through hydrogen-bond interactions in different chemical transformations, thus substituting hazardous reagents and solvents. The search for novel and more environmentally friendly catalysts is an area of interest of pharmaceutical chemists, and therefore, the efforts made in the application of DESs as catalysts in the synthesis of APIs or its precursors are described, focusing mainly on condensations, nucleophilic additions to carbonyl moieties, and multicomponent reactions.
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Citation: Falcini, C.; Gonzalo, G.d. Deep Eutectic Solvents as Catalysts in the Synthesis of Active Pharmaceutical Ingredients and Precursors. Catalysts 2024,14, 120. https://doi.org/10.3390/catal14020120 Academic Editor: Antonio Salomone Received: 12 January 2024 Revised: 30 January 2024 Accepted: 31 January 2024 Published: 2 February 2024 Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). catalysts Review Deep Eutectic Solvents as Catalysts in the Synthesis of Active Pharmaceutical Ingredients and Precursors Chiara Falcini and Gonzalo de Gonzalo * Departamento de Química Orgánica, Universidad de Sevilla, c/Profesor García González 1, 41012 Sevilla, Spain; [email protected] *Correspondence: [email protected] Abstract: Deep Eutectic Solvents (DESs) have appeared in recent years as an appealing alternative to classical organic solvents, due to their valuable environmental properties. In addition, these compounds, formed by the combination of one hydrogen bond donor with a hydrogen bond acceptor at a defined stoichiometric ratio, present other valuable activities not only as a reaction medium. DESs can also be employed as catalysts through hydrogen-bond interactions in different chemical transformations, thus substituting hazardous reagents and solvents. The search for novel and more environmentally friendly catalysts is an area of interest of pharmaceutical chemists, and therefore, the efforts made in the application of DESs as catalysts in the synthesis of APIs or its precursors are described, focusing mainly on condensations, nucleophilic additions to carbonyl moieties, and multicomponent reactions. Keywords: deep eutectic solvents; APIs; catalysis; multicomponent reactions; condensations 1. Introduction The chemical industry, especially the pharmaceutical one, has a great impact on the environment. Several tons of waste are generated to produce Active Pharmaceutical Ingredients (APIs) and their precursors [ 1 ], thus leading to high E-factor values for these processes (from 25 to higher than 100), expressed as kg of waste generated by kg of product obtained [ 2 , 3 ]. The main part of these wastes is formed by the solvent(s), employed both as the reaction medium and for the product downstream. When referring to the synthesis of APIs and their precursors, it can be estimated that more than 80% of the wastes generated correspond to solvents [ 4 ]. As stated by the Twelve Principles of Green Chemistry [ 5 , 6 ], which establish a set of rules for the development of more sustainable chemical synthesis, the use of safer solvents is an area of main interest to develop sustainable procedures. Thus, classical organic solvents commonly employed in organic synthesis are not environmentally friendly, as they have relatively low boiling points and are highly flammable, toxic, and harmful for human health. The replacement of these hazardous solvents represents a challenge for chemists [ 7 ], with some more sustainable alternatives appearing in recent years such as biobased solvents [ 8 – 10 ], supercritical fluids [ 11 , 12 ], or neoteric solvents. In this last category, we can include ionic liquids (ILs) [ 13 – 15 ], widely used at the beginning of this century as solvents for several valuable transformations. Nowadays, studies performed on these compounds have shown that they present serious environmental issues, and Deep Eutectic Solvents (DESs) have appeared as a valuable alternative [16–20]. The term “Deep Eutectic Solvent” was introduced for the first time in 2003 by Abbott and collaborators [ 21 ], with the idea of preparing liquid mixtures at room temperature, using solid compounds with high melting points as the starting material. Since this seminal work, interest in this type of solvent has increased considerably both at the industrial and academic level, as demonstrated by the number of publications on this topic in the last Catalysts 2024,14, 120. https://doi.org/10.3390/catal14020120 https://www.mdpi.com/journal/catalysts
Catalysts 2024,14, 120 2 of 16 two decades. When they appeared in the literature, DESs were considered as a new class of ILs, due to their similar characteristics and properties. However, classic ILs and DESs represent two different types of solvents [ 22 ]. A DES, in fact, is a combination of two or more solid components which, through the formation of hydrogen bonds, create an eutectic mixture, defined as a mixture of substances that has a lower melting point than that of the individual components; by contrast, classic ILs are compounds completely composed of ions. In most cases, DESs are obtained by mixing a hydrogen bond acceptor (HBA), typically ammonium, phosphonium, and natural amino acid salts, with a hydrogen bond donor (HBD) such as sugars, polyols, ureas, and natural carboxylic acids; therefore, all raw materials can be derived from renewable sources [23–25]. DESs present valuable properties such as high thermal stability, low vapor pressure, very low toxicity, and high biodegradability. These solvents are easy to prepare, with a complete atom economy, presenting tunability of their physicochemical properties. Therefore, DESs are appropriate for employment in pharmaceutical chemistry with different purposes, thus also demonstrating a high versatility. Of course, DESs can be employed as solvents with improved properties for both the synthesis of APIs and precursors [ 26 , 27 ] and for the solubilization of drugs [ 28 ]. In addition, the recent development of the so-called therapeutic DESs (THEDES) has been observed, in which one of the components is a bioactive compound or a pharmaceutical ingredient which can be released in the appropriate environment [ 29 ]. Apart from these roles, DESs have demonstrated their activity as catalysts in different reactions [ 30 – 34 ]. As these compounds are formed by hydrogen bond donors and acceptors (Figure 1), they can mediate a wide set of transformations that occur through hydrogen bond catalysis, an area of high interest in organic chemistry [ 35 – 37 ]. Hydrogen bonding to an electrophile increases the reactivity of this compound, as it is more activated to a nucleophilic attack. Hydrogen bonding catalysis is widely found in nature and can be employed for electrophile activation in several reactions. Thus, a set of processes including cycloadditions, condensations, multicomponent reactions, and redox can be carried out in the presence of different amounts of DESs acting as hydrogen-bond catalysts and as cosolvents. In the present review, we have focused on the efforts made for the application of different DESs as catalysts in the synthesis of molecules with pharmaceutical interest. Catalysts2024,14,xFORPEERREVIEW2of17 lasttwodecades.Whentheyappearedintheliterature,DESswereconsideredasanew classofILs,duetotheirsimilarcharacteristicsandproperties.However,classicILsand DESsrepresenttwodifferenttypesofsolvents[22].ADES,infact,isacombinationoftwo ormoresolidcomponentswhich,throughtheformationofhydrogenbonds,createan eutecticmixture,definedasamixtureofsubstancesthathasalowermeltingpointthan thatoftheindividualcomponents;bycontrast,classicILsarecompoundscompletely composedofions.Inmostcases,DESsareobtainedbymixingahydrogenbondacceptor (HBA),typicallyammonium,phosphonium,andnaturalaminoacidsalts,withahydrogenbonddonor(HBD)suchassugars,polyols,ureas,andnaturalcarboxylicacids;therefore,allrawmaterialscanbederivedfromrenewablesources[23–25]. DESspresentvaluablepropertiessuchashighthermalstability,lowvaporpressure, verylowtoxicity,andhighbiodegradability.Thesesolventsareeasytoprepare,witha completeatomeconomy,presentingtunabilityoftheirphysicochemicalproperties.Therefore,DESsareappropriateforemploymentinpharmaceuticalchemistrywithdifferent purposes,thusalsodemonstratingahighversatility.Ofcourse,DESscanbeemployedas solventswithimprovedpropertiesforboththesynthesisofAPIsandprecursors[26,27] andforthesolubilizationofdrugs[28].Inaddition,therecentdevelopmentofthesocalledtherapeuticDESs(THEDES)hasbeenobserved,inwhichoneofthecomponentsis abioactivecompoundorapharmaceuticalingredientwhichcanbereleasedintheappropriateenvironment[29].Apartfromtheseroles,DESshavedemonstratedtheiractivityas catalystsindifferentreactions[30–34].Asthesecompoundsareformedbyhydrogenbond donorsandacceptors(Figure1),theycanmediateawidesetoftransformationsthatoccur throughhydrogenbondcatalysis,anareaofhighinterestinorganicchemistry[35–37]. Hydrogenbondingtoanelectrophileincreasesthereactivityofthiscompound,asitis moreactivatedtoanucleophilicattack.Hydrogenbondingcatalysisiswidelyfoundin natureandcanbeemployedforelectrophileactivationinseveralreactions.Thus,asetof processesincludingcycloadditions,condensations,multicomponentreactions,andredox canbecarriedoutinthepresenceofdifferentamountsofDESsactingashydrogen-bond catalystsandascosolvents.Inthepresentreview,wehavefocusedontheeffortsmade fortheapplicationofdifferentDESsascatalystsinthesynthesisofmoleculeswithpharmaceuticalinterest. Figure1.Structureofthehydrogenbonddonor(HBD)andacceptor(HBA)componentsoftheDESs employedascatalystsinthepreparationofAPIsandprecursors. 2.DESsasCatalystsinCondensationProcesses β-Hydroxy-functionalizedderivativesarevaluableprecursorstoawidevarietyof compoundssuchasnitroalkenes,2-aminoalcohols,or2-nitroketones.Amongthoseβ-hy- droxycompounds,nitroaldolsorβ-nitroalcoholscanbeemployedasbuildingblocksin Figure 1. Structure of the hydrogen bond donor (HBD) and acceptor (HBA) components of the DESs employed as catalysts in the preparation of APIs and precursors. 2. DESs as Catalysts in Condensation Processes β -Hydroxy-functionalized derivatives are valuable precursors to a wide variety of compounds such as nitroalkenes, 2-aminoalcohols, or 2-nitroketones. Among those β - hydroxy compounds, nitroaldols or β -nitroalcohols can be employed as building blocks in pharmaceutical chemistry [ 38 ] and as synthons for the synthesis of natural products [ 39 ]. Thus, β -nitroalcohols can be easily reduced to 1,2-amino alcohols which are contained in many APIs [ 40 ]. Generally, nitroaldols are prepared using the Henry reaction (nitroaldol reaction), involving C–C bond formation between a nucleophilic nitroalkane and an elec-
Catalysts 2024,14, 120 3 of 16 trophilic aldehyde or ketone, a process developed at room temperature in the presence of a base [ 41 ]. However, the use of bases often leads to the formation of side products. In 2012, Singh et al. employed DESs as catalysts in the Henry reaction for the first time [ 42 ]. The choline chloride/urea [ChCl:U] (1:2) DES was tested as a catalyst (20% v/v) in methanol as the reaction medium, due to the ability of urea of catalyzing this type of process. A set of nitroaldols containing different aromatic and heteroaromatic groups were synthesized with yields between 69 and 95% after 5–60 min (Scheme 1). The recycling of the DES in the reaction of 4-nitrobenzaldehyde with nitromethane was studied, and after five cycles, the yield was observed to only decrease from 95% to 90%. The authors also investigated the synthesis of other β -hydroxy derivatives including β -hydroxy nitriles and β -hydroxy carboxylic acids employing DESs as catalysts. Different percentages of DESs for both syntheses were analyzed, and it was observed that for the preparation of β -hydroxy nitriles, a 30% v/vDES led to a 95% yield after 2 h and, for the preparation of β -hydroxy carboxylic acids, the best DES percentage was 40% v/v, achieving a 91% yield after 3 h. Catalysts2024,14,xFORPEERREVIEW3of17 pharmaceuticalchemistry[38]andassynthonsforthesynthesisofnaturalproducts[39]. Thus,β-nitroalcoholscanbeeasilyreducedto1,2-aminoalcoholswhicharecontainedin manyAPIs[40].Generally,nitroaldolsarepreparedusingtheHenryreaction(nitroaldol reaction),involvingC–Cbondformationbetweenanucleophilicnitroalkaneandanelectrophilicaldehydeorketone,aprocessdevelopedatroomtemperatureinthepresenceof abase[41].However,theuseofbasesoftenleadstotheformationofsideproducts.In 2012,Singhetal.employedDESsascatalystsintheHenryreactionforthefirsttime[42]. Thecholinechloride/urea[ChCl:U](1:2)DESwastestedasacatalyst(20%v/v)inmethanolasthereactionmedium,duetotheabilityofureaofcatalyzingthistypeofprocess.A setofnitroaldolscontainingdifferentaromaticandheteroaromaticgroupsweresynthesizedwithyieldsbetween69and95%after5–60min(Scheme1).TherecyclingoftheDES inthereactionof4-nitrobenzaldehydewithnitromethanewasstudied,andafterfivecycles,theyieldwasobservedtoonlydecreasefrom95%to90%.Theauthorsalsoinvestigatedthesynthesisofotherβ-hydroxyderivativesincludingβ-hydroxynitrilesandβhydroxycarboxylicacidsemployingDESsascatalysts.DifferentpercentagesofDESsfor bothsyntheseswereanalyzed,anditwasobservedthatforthepreparationofβ-hydroxy nitriles,a30%v/vDESledtoa95%yieldafter2hand,forthepreparationofβ-hydroxy carboxylicacids,thebestDESpercentagewas40%v/v,achievinga91%yieldafter3h. Scheme1.Henryreactionbetween(hetero)aromaticaldehydesandnitromethanecatalyzedby urea–basedDESs. α-Diazocarbonylcompoundsarewidelyusedasbuildingblocksfororganicsynthesis[43];moreover,theycanbeusedforthestructure-selectivealkylationofDNAandRNA [44].Amongthedifferentmethodologiesreportedintheliteratureforthepreparationof thesecompounds,theclassicmethodologyconsistsofthecondensationbetweenaldehydesandacyldiazomethanesinthepresenceofastrongbasesuchasLDAorsodium hydride.Thesemethodsrequireharshconditions,andothersyntheticroutesemploying heterogeneouscatalystshavebeeninvestigated,butthework-upprocessinthesereactionshaspresentedsomedrawbacks.Thus,in2017,Mirakietal.testedsomeDESsas catalystsandsolventsinthealdol-typecondensationofaromaticaldehydescontaining electron-withdrawingor-donatinggroups,heterocyclicaldehydes,andaliphaticaldehydeswithethyldiazoacetate(EDA)atroomtemperature(Scheme2)[45].Thesyntheses wereperformedusingpotassiumcarbonateasthebasemixedwithglyceroltoformthe DESatdifferentmolarratios,observingthebestresultsinthepresenceofa1:5ratioof basetoglycerol,leadingtofinalproductyieldsbetween60and96%.Whenaromaticand heterocyclicaldehydesweretested,thereactionswerecompletedafter2h,whereasaliphaticaldehydesrequired4h.Theuseofanα,β-unsaturatedaldehydesuchascinnamaldehydeledtothefinalproductwithmoderateyield(73%). Scheme2.DES-catalyzedcondensationofaldehydeswithethyldiazoacetate. Scheme 1. Henry reaction between (hetero)aromatic aldehydes and nitromethane catalyzed by urea–based DESs. α -Diazocarbonyl compounds are widely used as building blocks for organic synthesis [ 43 ]; moreover, they can be used for the structure-selective alkylation of DNA and RNA [ 44 ]. Among the different methodologies reported in the literature for the preparation of these compounds, the classic methodology consists of the condensation between aldehydes and acyldiazomethanes in the presence of a strong base such as LDA or sodium hydride. These methods require harsh conditions, and other synthetic routes employing heterogeneous catalysts have been investigated, but the work-up process in these reactions has presented some drawbacks. Thus, in 2017, Miraki et al. tested some DESs as catalysts and solvents in the aldol-type condensation of aromatic aldehydes containing electron-withdrawing or -donating groups, heterocyclic aldehydes, and aliphatic aldehydes with ethyl diazoacetate (EDA) at room temperature (Scheme 2) [ 45 ]. The syntheses were performed using potassium carbonate as the base mixed with glycerol to form the DES at different molar ratios, observing the best results in the presence of a 1:5 ratio of base to glycerol, leading to final product yields between 60 and 96%. When aromatic and heterocyclic aldehydes were tested, the reactions were completed after 2 h, whereas aliphatic aldehydes required 4 h. The use of an α , β -unsaturated aldehyde such as cinnamaldehyde led to the final product with moderate yield (73%). Catalysts2024,14,xFORPEERREVIEW3of17 pharmaceuticalchemistry[38]andassynthonsforthesynthesisofnaturalproducts[39]. Thus,β-nitroalcoholscanbeeasilyreducedto1,2-aminoalcoholswhicharecontainedin manyAPIs[40].Generally,nitroaldolsarepreparedusingtheHenryreaction(nitroaldol reaction),involvingC–Cbondformationbetweenanucleophilicnitroalkaneandanelectrophilicaldehydeorketone,aprocessdevelopedatroomtemperatureinthepresenceof abase[41].However,theuseofbasesoftenleadstotheformationofsideproducts.In 2012,Singhetal.employedDESsascatalystsintheHenryreactionforthefirsttime[42]. Thecholinechloride/urea[ChCl:U](1:2)DESwastestedasacatalyst(20%v/v)inmethanolasthereactionmedium,duetotheabilityofureaofcatalyzingthistypeofprocess.A setofnitroaldolscontainingdifferentaromaticandheteroaromaticgroupsweresynthesizedwithyieldsbetween69and95%after5–60min(Scheme1).TherecyclingoftheDES inthereactionof4-nitrobenzaldehydewithnitromethanewasstudied,andafterfivecycles,theyieldwasobservedtoonlydecreasefrom95%to90%.Theauthorsalsoinvestigatedthesynthesisofotherβ-hydroxyderivativesincludingβ-hydroxynitrilesandβhydroxycarboxylicacidsemployingDESsascatalysts.DifferentpercentagesofDESsfor bothsyntheseswereanalyzed,anditwasobservedthatforthepreparationofβ-hydroxy nitriles,a30%v/vDESledtoa95%yieldafter2hand,forthepreparationofβ-hydroxy carboxylicacids,thebestDESpercentagewas40%v/v,achievinga91%yieldafter3h. Scheme1.Henryreactionbetween(hetero)aromaticaldehydesandnitromethanecatalyzedby urea–basedDESs. α-Diazocarbonylcompoundsarewidelyusedasbuildingblocksfororganicsynthesis[43];moreover,theycanbeusedforthestructure-selectivealkylationofDNAandRNA [44].Amongthedifferentmethodologiesreportedintheliteratureforthepreparationof thesecompounds,theclassicmethodologyconsistsofthecondensationbetweenaldehydesandacyldiazomethanesinthepresenceofastrongbasesuchasLDAorsodium hydride.Thesemethodsrequireharshconditions,andothersyntheticroutesemploying heterogeneouscatalystshavebeeninvestigated,butthework-upprocessinthesereactionshaspresentedsomedrawbacks.Thus,in2017,Mirakietal.testedsomeDESsas catalystsandsolventsinthealdol-typecondensationofaromaticaldehydescontaining electron-withdrawingor-donatinggroups,heterocyclicaldehydes,andaliphaticaldehydeswithethyldiazoacetate(EDA)atroomtemperature(Scheme2)[45].Thesyntheses wereperformedusingpotassiumcarbonateasthebasemixedwithglyceroltoformthe DESatdifferentmolarratios,observingthebestresultsinthepresenceofa1:5ratioof basetoglycerol,leadingtofinalproductyieldsbetween60and96%.Whenaromaticand heterocyclicaldehydesweretested,thereactionswerecompletedafter2h,whereasaliphaticaldehydesrequired4h.Theuseofanα,β-unsaturatedaldehydesuchascinnamaldehydeledtothefinalproductwithmoderateyield(73%). Scheme2.DES-catalyzedcondensationofaldehydeswithethyldiazoacetate. Scheme 2. DES-catalyzed condensation of aldehydes with ethyl diazoacetate. Methylthiotriazolo[1,5-a]pyrimidines are compounds with antimicrobial activity [ 46 ], and different synthetic approaches have been performed for their preparation. In most cases, long reaction times are required and difficult work-ups must be performed using non-environmentally friendly catalysts. Therefore, a novel approach has recently been developed by employing DESs as catalysts for the preparation of these compounds through
Catalysts 2024,14, 120 4 of 16 a condensation process between 4-dimethylamino-1-aryl-3-alken-2-ones and 3-amino-5- methylthio-1H-1,2,4-triazole [ 47 ], as shown in Scheme 3. DESs containing ChCl as a hydrogen bond acceptor and different hydrogen bond donors were tested in this reaction. It was observed that by using the DES formed by ChCl and p-toluensulfonic acid (p-TsOH) in a molar ratio of 1:2, the desired product can be obtained with 90% yield after 5 min at 120 ◦ C. It is important to note that the molar ratio of the DES was essential for a high reactivity, as when ChCl:p-TsOH in a 1:1 ratio was tested, an incomplete conversion was observed. The reaction scope was checked by employing several enaminones as starting materials. After 5 min at 120 ◦ C, excellent yields of the final cyclic products were achieved (82–98%). The authors also performed mechanistic studies on this reaction, establishing that acid catalysis and solvation effects on the activated complex determined the regioselectivity of the process. Catalysts2024,14,xFORPEERREVIEW4of17 Methylthiotriazolo[1,5-a]pyrimidinesarecompoundswithantimicrobialactivity [46],anddifferentsyntheticapproacheshavebeenperformedfortheirpreparation.In mostcases,longreactiontimesarerequiredanddifficultwork-upsmustbeperformed usingnon-environmentallyfriendlycatalysts.Therefore,anovelapproachhasrecently beendevelopedbyemployingDESsascatalystsforthepreparationofthesecompounds throughacondensationprocessbetween4-dimethylamino-1-aryl-3-alken-2-onesand3- amino-5-methylthio-1H-1,2,4-triazole[47],asshowninScheme3.DESscontainingChCl asahydrogenbondacceptoranddifferenthydrogenbonddonorsweretestedinthisreaction.ItwasobservedthatbyusingtheDESformedbyChClandp-toluensulfonicacid (p-TsOH)inamolarratioof1:2,thedesiredproductcanbeobtainedwith90%yieldafter 5minat120°C.ItisimportanttonotethatthemolarratiooftheDESwasessentialfora highreactivity,aswhenChCl:p-TsOHina1:1ratiowastested,anincompleteconversion wasobserved.Thereactionscopewascheckedbyemployingseveralenaminonesasstartingmaterials.After5minat120°C,excellentyieldsofthefinalcyclicproductswere achieved(82–98%).Theauthorsalsoperformedmechanisticstudiesonthisreaction,establishingthatacidcatalysisandsolvationeffectsontheactivatedcomplexdetermined theregioselectivityoftheprocess. Scheme3.Synthesisofmethylthiotriazolo[1,5-a]pyrimidinesbyaDES-catalyzedcondensationbetween4-dimethylamino-1-aryl-3-alken-2-onesand3-amino-5-methylthio-1H-1,2,4-triazole. 3.DESsasCatalystsinMulticomponentReactions Multicomponentreactions(MCRs)arepowerfulsyntheticmethodologiesthatinvolvethesimultaneousreactionofthreeormorereactantstoproduceasingle,morecomplexproduct.MCRsofferseveraladvantagesinorganicsynthesis,suchashighatomeconomy,stepeconomy,andarapidgenerationofmolecularcomplexity.Thesereactionshave becomepopularinmedicinalchemistry,combinatorialchemistry,andthesynthesisof complexnaturalproducts[48–50].Sincetheirdiscoveryatthebeginningofthiscentury, DESshavebeendescribedasmediatorsforthistypeofreaction. 2,3-Dihydroquinazolinonesareheterocycliccompoundswidelyusedinthepreparationofpharmaceuticalssuchasmetolazone,quinethazone,raltitrexed,andfenquizone, duetothebiologicalandpharmaceuticalactivitiesthattheseheterocyclespresent[51,52]. Generally,thesemoleculesaresynthesizedbyamulticomponentreactionbetweenisatoic anhydride,analdehydeoraketone,andaromaticamines,usingacidcatalystsinorganic media.Thisprocedurepresentsseverallimitations,duetotheuseofnon-environmentally friendlycatalystsandsolvents,strongacidicconditions,prolongedreactiontimes,and lowyields.In2012,Loboetal.testeddifferentDESsat20%v/vascatalystsinthereaction of4-chlorobenzaldehydewith4-methylanilineandisaoticanhydride,developingamore sustainableprocess[53].AmongtheeutecticsolventsanalyzedwereChCl:malonicacid (MA),ChCl:glycerol(Gly),ChCl:U,andChCl:ZnCl2,usingmethanolasreactionmediaat 65°Cfor2h(Scheme4A).ThebestresultwasachievedinthepresenceofChCl:MA(1:1) witha94%yield.TheuseofthisDESwasextendedforthepreparationofother2,3-dihy- droquinazolin-4(1H)-onesstartingfromaromaticandheteroaromaticaldehydesanddifferentanilines,achievingyieldsfrom80to95%atshortreactiontimes(2–3h).DEScould Scheme 3. Synthesis of methylthiotriazolo[1,5-a]pyrimidines by a DES-catalyzed condensation between 4-dimethylamino-1-aryl-3-alken-2-ones and 3-amino-5-methylthio-1H-1,2,4-triazole. 3. DESs as Catalysts in Multicomponent Reactions Multicomponent reactions (MCRs) are powerful synthetic methodologies that involve the simultaneous reaction of three or more reactants to produce a single, more complex product. MCRs offer several advantages in organic synthesis, such as high atom economy, step economy, and a rapid generation of molecular complexity. These reactions have become popular in medicinal chemistry, combinatorial chemistry, and the synthesis of complex natural products [ 48 – 50 ]. Since their discovery at the beginning of this century, DESs have been described as mediators for this type of reaction. 2,3-Dihydroquinazolinones are heterocyclic compounds widely used in the preparation of pharmaceuticals such as metolazone, quinethazone, raltitrexed, and fenquizone, due to the biological and pharmaceutical activities that these heterocycles present [ 51 , 52 ]. Generally, these molecules are synthesized by a multicomponent reaction between isatoic anhydride, an aldehyde or a ketone, and aromatic amines, using acid catalysts in organic media. This procedure presents several limitations, due to the use of non-environmentally friendly catalysts and solvents, strong acidic conditions, prolonged reaction times, and low yields. In 2012, Lobo et al. tested different DESs at 20% v/vas catalysts in the reaction of 4-chlorobenzaldehyde with 4-methylaniline and isaotic anhydride, developing a more sustainable process [ 53 ]. Among the eutectic solvents analyzed were ChCl:malonic acid (MA), ChCl:glycerol (Gly), ChCl:U, and ChCl:ZnCl 2 , using methanol as reaction media at 65 ◦ C for 2 h (Scheme 4A). The best result was achieved in the presence of ChCl:MA (1:1) with a 94% yield. The use of this DES was extended for the preparation of other 2,3-dihydroquinazolin-4(1H)-ones starting from aromatic and heteroaromatic aldehydes and different anilines, achieving yields from 80 to 95% at short reaction times (2–3 h). DES could be recycled up to four times through methanol evaporation after each reaction, with no loss in system activity.
Catalysts 2024,14, 120 5 of 16 Catalysts2024,14,xFORPEERREVIEW5of17 berecycleduptofourtimesthroughmethanolevaporationaftereachreaction,withno lossinsystemactivity. Scheme4.(A)Synthesisof2,3-dihydroquinazolinonesthroughanMCRbetweenisatoicanhydride, aldehydes,andaromaticaminescatalyzedbyChCl:MA(1:1).(B)Preparationof2,3-dihydro- quinazolinonesusingammoniumacetateasnitrogensourceandU:ZnCl2(3.5:1)ascatalyst.(C)SynthesisoftryptanthrincatalyzedbyU:ZnCl2(3.5:1)usingisatoicanhydrideandisatin. TheuseofDESsascatalystsandsolventsforthesynthesisof2,3-dihydroquinazoli- noneswasfurtherinvestigatedin2019byPeña-Solórzanoetal.[54].AsetofDESswere testedinthereactionbetweenanaldehyde(benzaldehyde)andisatoicanhydrideinthe presenceofanitrogensource(NH4OAc)atdifferenttemperatures(60–110°C)for5–120 min.Thebestconditionwasat60°Cfor5mininthepresenceofurea/ZnCl2(3.5:1)asthe DES,thusobtaininga97%yieldinthedesiredproduct(Scheme4B).TheauthorsestablishedthatthebetterperformanceoftheDEScanbeattributedtotheLewischaracteristic ofthemixturethatenhancestheelectrophilicityofbothcarbonylgroupspresentinthe reactionmedium(isatoicanhydrideandaldehyde).Thereactionwasextendedtothesynthesisofother2,3-dihydroquinazolin-4(1H)-onesbearingdifferentsubstituents,achieving excellentyieldsaftershortreactiontimesinallcases. Theauthorsfinallydescribedthepreparationofthebiologicallyactivedihydroquinazolinones.Followingthepreviouslydescribedmethodologyusingaliphaticaldehydes,ammoniumacetateandisatoicanhydridedidnotaffordthefinalcompounds,due totheformationofseveralsideproducts.Thus,forthepreparationofthealkaloidtryptanthrin,thereactionbetweenisatoicanhydrideandisatinfor30mincatalyzedbytheDES ledtothefinalproductwithhighyield(91%,Scheme4C)[54]. In2013,HeandcoworkersdescribedtheuseofaBrönsted-typeDES(cholinechloride:p-TsOHacid1:1)fortheone-potsynthesisof2H-indazolo[2.1.b]phthalazinetriones [55],whicharecompoundsemployedasanticonvulsants,cardiotonics,andvasorelaxants [56].Amongthedifferentsyntheticmethodologiesfortheirpreparation,theMCRof phthalhydrazide,analdehydeanddimedoneinthepresenceofacatalysthaverecently gainedstronginterest.p-TsOHcanbeemployedasacatalystforthisprocess,butitis Scheme 4. (A) Synthesis of 2,3-dihydroquinazolinones through an MCR between isatoic anhydride, aldehydes, and aromatic amines catalyzed by ChCl:MA (1:1). (B) Preparation of 2,3- dihydroquinazolinones using ammonium acetate as nitrogen source and U:ZnCl 2 (3.5:1) as catalyst. (C) Synthesis of tryptanthrin catalyzed by U:ZnCl2(3.5:1) using isatoic anhydride and isatin. The use of DESs as catalysts and solvents for the synthesis of 2,3-dihydroquinazolinones was further investigated in 2019 by Peña-Solórzano et al. [ 54 ]. A set of DESs were tested in the reaction between an aldehyde (benzaldehyde) and isatoic anhydride in the presence of a nitrogen source (NH 4 OAc) at different temperatures (60–110 ◦ C) for 5–120 min. The best condition was at 60 ◦ C for 5 min in the presence of urea/ZnCl 2 (3.5:1) as the DES, thus obtaining a 97% yield in the desired product (Scheme 4B). The authors established that the better performance of the DES can be attributed to the Lewis characteristic of the mixture that enhances the electrophilicity of both carbonyl groups present in the reaction medium (isatoic anhydride and aldehyde). The reaction was extended to the synthesis of other 2,3-dihydroquinazolin-4(1H)-ones bearing different substituents, achieving excellent yields after short reaction times in all cases. The authors finally described the preparation of the biologically active dihydroquinazolinones. Following the previously described methodology using aliphatic aldehydes, ammonium acetate and isatoic anhydride did not afford the final compounds, due to the formation of several side products. Thus, for the preparation of the alkaloid tryptanthrin, the reaction between isatoic anhydride and isatin for 30 min catalyzed by the DES led to the final product with high yield (91%, Scheme 4C) [54]. In 2013, He and coworkers described the use of a Brönsted-type DES (choline chloride:p-TsOH acid 1:1) for the one-pot synthesis of 2H-indazolo[2.1.b]phthalazinetriones [ 55 ], which are compounds employed as anticonvulsants, cardiotonics, and vasorelaxants [ 56 ]. Among the different synthetic methodologies for their preparation, the MCR of phthalhydrazide, an aldehyde and dimedone in the presence of a catalyst have recently gained strong interest. p-TsOH can be employed as a catalyst for this process, but it is deliquescent and difficult to recover. Therefore, the DES formed by ChCl/p-TsOH (1:1) was prepared and employed as the catalyst for this reaction. The best result was achieved when the MCR was developed in methanol containing 15% v/vof the DES at reflux for 4 h, leading
Catalysts 2024,14, 120 6 of 16 to an 88% yield of the benzofused product. The synthetic methodology in the optimized conditions was extended to the preparation of a wide set of final products starting from different substituted aromatic aldehydes, with yields from 85 to 93% (Scheme 5). Catalysts2024,14,xFORPEERREVIEW6of17 deliquescentanddifficulttorecover.Therefore,theDESformedbyChCl/p‐TsOH(1:1) waspreparedandemployedasthecatalystforthisreaction.Thebestresultwasachieved whentheMCRwasdevelopedinmethanolcontaining15%v/voftheDESatrefluxfor4 h,leadingtoan88%yieldofthebenzofusedproduct.Thesyntheticmethodologyinthe optimizedconditionswasextendedtothepreparationofawidesetoffinalproductsstartingfromdifferentsubstitutedaromaticaldehydes,withyieldsfrom85to93%(Scheme5). Scheme5.MCRforthepreparationof2H-indazolo[2.1.b]phthalazinetrionesstartingfromphthalhydrazide,aldehydes,anddimedoneinthepresenceofChCl:p-TsOH(1:1)ascatalyst. TheGroebke–Blackburn–Bienayme(GBB)reactionconsistsofanMCRbetweenan aldehyde,a2-aminoazide,andanisocyanidecatalyzedbyanacid,yieldingthefinalfused imidazolederivatives[57].Onetypeofsuchderivativesaretheimidazo[1,2-a]pyridines, whicharevaluablestructurespresentinseveralcompoundswithpharmaceuticalactivity includingzolpidem,alpidem,andzolimidine[58].Initialstudieswerecarriedoutinthe GBBreactionofbenzaldehyde,2-aminopyridine,andcyclohexylisocyanideinthepresenceofDESspresentingdifferentcompositions,achievingthefinalproductwiththehighestyieldwhenusingChCl:Urea(1:2)at90°C,asthefinalproductwasrecoveredwith80% yield[59](Scheme6).ThesebestconditionswereappliedfortheGBBreactionemploying differentaldehydes,2-aminoheterocyclesandiscocyanides.Thereactionsproceededwith goodyieldsaftershorttimes(1–2h)whenusing2-aminopyrazinesandsubstituted2-ami- nopyridines.Awidesetofaromaticaldehydespresentingelectron-donatingorelectronwithdrawinggroupscanalsobeemployedinthisprocedure,andevenheteroaromatic andpolycyclicaldehydesaresuitableforthereaction,leadingtohighyields.Alkylisocyanidescanalsobeemployedwhilemaintaininggoodactivities.DESrecyclingwasstudied inthemodelreaction.Afteroneprocesswascompleted,thereactionmixturewasdissolvedinwaterandtheproductwasextractedwithethylacetate(EtOAc).Evaporationof thewaterat80°CallowedtheDEStoberecoveredforafurthercycle.Afterfourcycles, onlyaslightdecreaseinthereactionyieldwasobserved. Scheme6.Groebke–Blackburn–Bienaymereactionforsynthesizingimidazo[1,2-a]pyridinesusing ChCl:U(1:2)ascatalyst. 3,4-Dihydropyrimidin-2(1H)-onesorthiones(DHPMs)arevaluablemoleculesthat canbeemployedasantibacterials,antivirals,calciumantagonists,andantihypertensives Scheme 5. MCR for the preparation of 2H-indazolo[2.1.b]phthalazinetriones starting from phthalhydrazide, aldehydes, and dimedone in the presence of ChCl:p-TsOH (1:1) as catalyst. The Groebke–Blackburn–Bienayme (GBB) reaction consists of an MCR between an aldehyde, a 2-aminoazide, and an isocyanide catalyzed by an acid, yielding the final fused imidazole derivatives [ 57 ]. One type of such derivatives are the imidazo[1,2-a]pyridines, which are valuable structures present in several compounds with pharmaceutical activity including zolpidem, alpidem, and zolimidine [ 58 ]. Initial studies were carried out in the GBB reaction of benzaldehyde, 2-aminopyridine, and cyclohexyl isocyanide in the presence of DESs presenting different compositions, achieving the final product with the highest yield when using ChCl:Urea (1:2) at 90 ◦ C, as the final product was recovered with 80% yield [ 59 ] (Scheme 6). These best conditions were applied for the GBB reaction employing different aldehydes, 2-aminoheterocycles and iscocyanides. The reactions proceeded with good yields after short times (1–2 h) when using 2-aminopyrazines and substituted 2-aminopyridines. A wide set of aromatic aldehydes presenting electrondonating or electron-withdrawing groups can also be employed in this procedure, and even heteroaromatic and polycyclic aldehydes are suitable for the reaction, leading to high yields. Alkyl isocyanides can also be employed while maintaining good activities. DES recycling was studied in the model reaction. After one process was completed, the reaction mixture was dissolved in water and the product was extracted with ethyl acetate (EtOAc). Evaporation of the water at 80 ◦ C allowed the DES to be recovered for a further cycle. After four cycles, only a slight decrease in the reaction yield was observed. Catalysts2024,14,xFORPEERREVIEW6of17 deliquescentanddifficulttorecover.Therefore,theDESformedbyChCl/p‐TsOH(1:1) waspreparedandemployedasthecatalystforthisreaction.Thebestresultwasachieved whentheMCRwasdevelopedinmethanolcontaining15%v/voftheDESatrefluxfor4 h,leadingtoan88%yieldofthebenzofusedproduct.Thesyntheticmethodologyinthe optimizedconditionswasextendedtothepreparationofawidesetoffinalproductsstartingfromdifferentsubstitutedaromaticaldehydes,withyieldsfrom85to93%(Scheme5). Scheme5.MCRforthepreparationof2H-indazolo[2.1.b]phthalazinetrionesstartingfromphthalhydrazide,aldehydes,anddimedoneinthepresenceofChCl:p-TsOH(1:1)ascatalyst. TheGroebke–Blackburn–Bienayme(GBB)reactionconsistsofanMCRbetweenan aldehyde,a2-aminoazide,andanisocyanidecatalyzedbyanacid,yieldingthefinalfused imidazolederivatives[57].Onetypeofsuchderivativesaretheimidazo[1,2-a]pyridines, whicharevaluablestructurespresentinseveralcompoundswithpharmaceuticalactivity includingzolpidem,alpidem,andzolimidine[58].Initialstudieswerecarriedoutinthe GBBreactionofbenzaldehyde,2-aminopyridine,andcyclohexylisocyanideinthepresenceofDESspresentingdifferentcompositions,achievingthefinalproductwiththehighestyieldwhenusingChCl:Urea(1:2)at90°C,asthefinalproductwasrecoveredwith80% yield[59](Scheme6).ThesebestconditionswereappliedfortheGBBreactionemploying differentaldehydes,2-aminoheterocyclesandiscocyanides.Thereactionsproceededwith goodyieldsaftershorttimes(1–2h)whenusing2-aminopyrazinesandsubstituted2-ami- nopyridines.Awidesetofaromaticaldehydespresentingelectron-donatingorelectronwithdrawinggroupscanalsobeemployedinthisprocedure,andevenheteroaromatic andpolycyclicaldehydesaresuitableforthereaction,leadingtohighyields.Alkylisocyanidescanalsobeemployedwhilemaintaininggoodactivities.DESrecyclingwasstudied inthemodelreaction.Afteroneprocesswascompleted,thereactionmixturewasdissolvedinwaterandtheproductwasextractedwithethylacetate(EtOAc).Evaporationof thewaterat80°CallowedtheDEStoberecoveredforafurthercycle.Afterfourcycles, onlyaslightdecreaseinthereactionyieldwasobserved. Scheme6.Groebke–Blackburn–Bienaymereactionforsynthesizingimidazo[1,2-a]pyridinesusing ChCl:U(1:2)ascatalyst. 3,4-Dihydropyrimidin-2(1H)-onesorthiones(DHPMs)arevaluablemoleculesthat canbeemployedasantibacterials,antivirals,calciumantagonists,andantihypertensives Scheme 6. Groebke–Blackburn–Bienayme reaction for synthesizing imidazo[1,2-a]pyridines using ChCl:U (1:2) as catalyst. 3,4-Dihydropyrimidin-2(1H)-ones or thiones (DHPMs) are valuable molecules that can be employed as antibacterials, antivirals, calcium antagonists, and antihypertensives [ 60 ]. These compounds can be prepared through a Biginelli reaction [ 61 ], employing β -ketoesters, aldehydes, and (thio)urea in the presence of different catalysts, but in almost all the cases, sensitive conditions and hazardous reagents are required [ 62 ]. In 2019, a novel catalytic
Catalysts 2024,14, 120 7 of 16 approach was developed by using DESs for mediating this transformation [ 63 ], as shown in Scheme 7. Several ChCl-based DESs, presenting different types of hydrogen bond donors including p-TsOH, trichloroacetic acid (TCA), monochloroacetic acid (MCA), propionic acid (PA), and ethylene glycol (EG), were tested in the reaction of benzaldehyde with ethyl acetoacetate and urea at 70 ◦ C. After 40 min, an 88% yield of the final product was achieved in the presence of ChCl:p-TsOH (1:2). The amount of catalysts was analyzed, and a ratio of 0.6 mmol of DES per 2.0 mmol of aldehyde and ethyl acetoacetate was observed, which was optimal in order to obtain the highest yields. If the DES amount increased, the yield decreased, probably due to the high acidity of the catalyst. The scope of the reaction was extended to other aldehydes and β -ketoesters, observing that it was suitable for a wide set of aromatic aldehydes, different β -diketonates, and urea or thiourea to obtain DHPMs with generally high yields (78–92%), except for ethyl benzoylacetate, for which no reactivity was observed. The reaction was also tested with aliphatic aldehydes, observing a 52% yield in the process between n-butylaldehyde, ethyl acetoacetate, and ureas, whereas a 48% yield was achieved in the presence of hexaldehyde. The recycling of ChCl:p-TsOH (1:2) was analyzed in the model reaction with benzaldehyde, ethyl acetate, and urea at 70 ◦ C, evaporating the solvent after each stage and employing the reused DES for a further cycle. Catalysts2024,14,xFORPEERREVIEW7of17 [60].ThesecompoundscanbepreparedthroughaBiginellireaction[61],employingβketoesters,aldehydes,and(thio)ureainthepresenceofdifferentcatalysts,butinalmost allthecases,sensitiveconditionsandhazardousreagentsarerequired[62].In2019,a novelcatalyticapproachwasdevelopedbyusingDESsformediatingthistransformation [63],asshowninScheme7.SeveralChCl-basedDESs,presentingdifferenttypesofhydrogenbonddonorsincludingp-TsOH,trichloroaceticacid(TCA),monochloroaceticacid (MCA),propionicacid(PA),andethyleneglycol(EG),weretestedinthereactionofbenzaldehydewithethylacetoacetateandureaat70°C.After40min,an88%yieldofthefinal productwasachievedinthepresenceofChCl:p-TsOH(1:2).Theamountofcatalystswas analyzed,andaratioof0.6mmolofDESper2.0mmolofaldehydeandethylacetoacetate wasobserved,whichwasoptimalinordertoobtainthehighestyields.IftheDESamount increased,theyielddecreased,probablyduetothehighacidityofthecatalyst.Thescope ofthereactionwasextendedtootheraldehydesandβ-ketoesters,observingthatitwas suitableforawidesetofaromaticaldehydes,differentβ-diketonates,andureaorthiourea toobtainDHPMswithgenerallyhighyields(78–92%),exceptforethylbenzoylacetate,for whichnoreactivitywasobserved.Thereactionwasalsotestedwithaliphaticaldehydes, observinga52%yieldintheprocessbetweenn-butylaldehyde,ethylacetoacetate,and ureas,whereasa48%yieldwasachievedinthepresenceofhexaldehyde.Therecyclingof ChCl:p-TsOH(1:2)wasanalyzedinthemodelreactionwithbenzaldehyde,ethylacetate, andureaat70°C,evaporatingthesolventaftereachstageandemployingthereusedDES forafurthercycle. Scheme7.BiginellireactionforthepreparationofDHPMscatalyzedbyChCl:p-TsOH(1:2). Thepyrazolo[3,4-b]pyridinemoietyappearsinawidesetofvaluablecompounds [64,65].Aclassofthesecompounds,thespirooxindoles,arepartofnaturalalkaloidsand scaffoldsforthedevelopmentofantivirals[66].In2019,theirsynthesiswasdescribedby usingDESsascatalystsinanMCRinvolving1H-pyrazol-5-amine,anisatin,andanenolizableC/H-activatedcompoundinthepresenceofmicrowaveirradiation[67].Different choline-chloride-basedDESsweretestedinthemodelreaction,consistingofthetreatment of1,3-diphenyl-1H-pyrazol-5-amine,isatin,and5,5-dimethylcyclohexane-1,3-dioneat equimolarquantitiesunder500Wofirradiationat60°C(Scheme8).Thehighestyields wereobservedinthepresenceofalacticacid(LA)-basedDES,focusingonChCl:LA.When theChCl:LAratiowas1:1,an86%yieldwasobservedafter20min,whereastheuseof ChCl:LA(1:2)affordedthedesiredproductin95%yieldatthesametime.Thisreaction wasscaledupto100mmol,maintainingsuchexcellentresults.Theauthorsalsoperformedrecyclingofthecatalysts,observingonlyaslightdecreaseinthereactionyield (78%)aftereightcycles.Asetofisatinsreactedwith1,3-diphenyl-1H-pyrazol-5-amineand 5,5-dimethylcyclohexane-1,3-dione,leadingtothefinalcompoundswithhighyields,despitethepresenceofdifferentsubstituentsinthestartingmaterials.Also,1H-pyrazol-5- aminesweretoleratedinthisreaction.OtherenolizableC−H-activatedcompoundssuch asbarbituricacid,cyclopentane-1,3-dione,cyclohexane-1,3-dione,and1,3-indanedione wereanalyzedinthisreaction,recoveringthecorrespondingpyrazolo[3,4-b]quinoline spirooxindolesinexcellentyields. Scheme 7. Biginelli reaction for the preparation of DHPMs catalyzed by ChCl:p-TsOH (1:2). The pyrazolo[3,4-b]pyridine moiety appears in a wide set of valuable compounds [ 64 , 65 ]. A class of these compounds, the spirooxindoles, are part of natural alkaloids and scaffolds for the development of antivirals [ 66 ]. In 2019, their synthesis was described by using DESs as catalysts in an MCR involving 1H-pyrazol-5-amine, an isatin, and an enolizable C/H-activated compound in the presence of microwave irradiation [ 67 ]. Different choline-chloride-based DESs were tested in the model reaction, consisting of the treatment of 1,3-diphenyl-1H-pyrazol- 5-amine, isatin, and 5,5-dimethylcyclohexane-1,3-dione at equimolar quantities under 500 W of irradiation at 60 ◦ C (Scheme 8). The highest yields were observed in the presence of a lactic acid (LA)-based DES, focusing on ChCl:LA. When the ChCl:LA ratio was 1:1, an 86% yield was observed after 20 min, whereas the use of ChCl:LA (1:2) afforded the desired product in 95% yield at the same time. This reaction was scaled up to 100 mmol, maintaining such excellent results. The authors also performed recycling of the catalysts, observing only a slight decrease in the reaction yield (78%) after eight cycles. A set of isatins reacted with 1,3-diphenyl-1H-pyrazol-5-amine and 5,5-dimethylcyclohexane-1,3-dione, leading to the final compounds with high yields, despite the presence of different substituents in the starting materials. Also, 1H-pyrazol-5-amines were tolerated in this reaction. Other enolizable C − H-activated compounds such as barbituric acid, cyclopentane-1,3-dione, cyclohexane-1,3- dione, and 1,3-indanedione were analyzed in this reaction, recovering the corresponding pyrazolo[3,4-b]quinoline spirooxindoles in excellent yields.
Catalysts 2024,14, 120 8 of 16 Catalysts2024,14,xFORPEERREVIEW8of17 Scheme8.Three-componentreactionbetween1H-pyrazol-5-amines,isatins,andenolizableC/H moleculestoachievespirooxindolesusingChCl:LA(1:2)ascatalyst. Benzofusedseven-memberedheterocyclessuchas1,4-benzodiazepinesand1,4-ben- zoxazepinespresentstronginterestinmedicinalchemistryduetotheirbiologicalproperties[68,69].Someexamplesofthesestructuresarediazepamandchlordiazepoxide(antianxietydrugs),andothercompoundspresentactivitysuchashistaminereceptoragonists, calciumagonists,andanalgesics.In2016,astudydescribedthepreparationofthesecompoundsviaathree-componentreactioninvolvingano-phenylenediamineor2-aminophe- nol,2-dimedone,andsomearomaticaldehydesinthepresenceofChCl:U(1:2)asacatalystandsolvent[70](Scheme9).Ingeneral,benzodiazepineswereachievedinhigher yields(80–94%)andshorterreactiontimesthanthebenzoxazepines(68–88%yields).It wasalsoobservedthattheuseofaromaticaldehydescontainingelectron-withdrawing groupsledtobetterresultswithrespecttothosewithelectron-donatinggroups.TheDES canbesimplyrecycledbydissolvingthereactionmixtureinwaterandfilteringtheinsolubleproduct.Afterwaterevaporation,theDESwasemployedagain,showingthatit maintainsitsactivityforfourcycles. Scheme9.Three-componentreactionforthepreparationofbenzodiazepinesandbenzoxazepines catalyzedbyChCl:U(1:2). Imidazoindolesandpyrroloimidazolesarestructuresthatpresentawidesetofpharmacologicalproperties,beingemployedasantimicrobial,antiviral,orimmunosuppressivecompounds[71,72].ThesemoleculescanbepreparedusingPd-catalyzedreactions, butthismethodpresentssomelimitations.SomeDESshavebeenrecentlyemployedas catalystsandsolventsinthethree-componentMCRprocedureusinganaldehyde,a1,3- diketone,andhydantoin,achievingthebestresultwhenusingguanidiniumchloride (GuHCl)/urea(1:2)at80°C[73],asshowninScheme10.After30min,a95%yieldofthe desiredcompoundwasobtained.ArecyclingstudyoftheDESshowedthatthesolvent/catalystcanbeemployedforsixreactionswithoutlossincatalyticactivity. Scheme 8. Three-component reaction between 1H-pyrazol-5-amines, isatins, and enolizable C/H molecules to achieve spirooxindoles using ChCl:LA (1:2) as catalyst. Benzofused seven-membered heterocycles such as 1,4-benzodiazepines and 1,4-benzoxazepines present strong interest in medicinal chemistry due to their biological properties [ 68 , 69 ]. Some examples of these structures are diazepam and chlordiazepoxide (anti-anxiety drugs), and other compounds present activity such as histamine receptor agonists, calcium agonists, and analgesics. In 2016, a study described the preparation of these compounds via a three-component reaction involving an o-phenylenediamine or 2- aminophenol, 2-dimedone, and some aromatic aldehydes in the presence of ChCl:U (1:2) as a catalyst and solvent [ 70 ] (Scheme 9). In general, benzodiazepines were achieved in higher yields (80–94%) and shorter reaction times than the benzoxazepines (68–88% yields). It was also observed that the use of aromatic aldehydes containing electron-withdrawing groups led to better results with respect to those with electron-donating groups. The DES can be simply recycled by dissolving the reaction mixture in water and filtering the insoluble product. After water evaporation, the DES was employed again, showing that it maintains its activity for four cycles. Catalysts2024,14,xFORPEERREVIEW8of17 Scheme8.Three-componentreactionbetween1H-pyrazol-5-amines,isatins,andenolizableC/H moleculestoachievespirooxindolesusingChCl:LA(1:2)ascatalyst. Benzofusedseven-memberedheterocyclessuchas1,4-benzodiazepinesand1,4-ben- zoxazepinespresentstronginterestinmedicinalchemistryduetotheirbiologicalproperties[68,69].Someexamplesofthesestructuresarediazepamandchlordiazepoxide(antianxietydrugs),andothercompoundspresentactivitysuchashistaminereceptoragonists, calciumagonists,andanalgesics.In2016,astudydescribedthepreparationofthesecompoundsviaathree-componentreactioninvolvingano-phenylenediamineor2-aminophe- nol,2-dimedone,andsomearomaticaldehydesinthepresenceofChCl:U(1:2)asacatalystandsolvent[70](Scheme9).Ingeneral,benzodiazepineswereachievedinhigher yields(80–94%)andshorterreactiontimesthanthebenzoxazepines(68–88%yields).It wasalsoobservedthattheuseofaromaticaldehydescontainingelectron-withdrawing groupsledtobetterresultswithrespecttothosewithelectron-donatinggroups.TheDES canbesimplyrecycledbydissolvingthereactionmixtureinwaterandfilteringtheinsolubleproduct.Afterwaterevaporation,theDESwasemployedagain,showingthatit maintainsitsactivityforfourcycles. Scheme9.Three-componentreactionforthepreparationofbenzodiazepinesandbenzoxazepines catalyzedbyChCl:U(1:2). Imidazoindolesandpyrroloimidazolesarestructuresthatpresentawidesetofpharmacologicalproperties,beingemployedasantimicrobial,antiviral,orimmunosuppressivecompounds[71,72].ThesemoleculescanbepreparedusingPd-catalyzedreactions, butthismethodpresentssomelimitations.SomeDESshavebeenrecentlyemployedas catalystsandsolventsinthethree-componentMCRprocedureusinganaldehyde,a1,3- diketone,andhydantoin,achievingthebestresultwhenusingguanidiniumchloride (GuHCl)/urea(1:2)at80°C[73],asshowninScheme10.After30min,a95%yieldofthe desiredcompoundwasobtained.ArecyclingstudyoftheDESshowedthatthesolvent/catalystcanbeemployedforsixreactionswithoutlossincatalyticactivity. Scheme 9. Three-component reaction for the preparation of benzodiazepines and benzoxazepines catalyzed by ChCl:U (1:2). Imidazoindoles and pyrroloimidazoles are structures that present a wide set of pharmacological properties, being employed as antimicrobial, antiviral, or immunosuppressive compounds [ 71 , 72 ]. These molecules can be prepared using Pd-catalyzed reactions, but this method presents some limitations. Some DESs have been recently employed as catalysts and solvents in the three-component MCR procedure using an aldehyde, a 1,3-diketone, and hydantoin, achieving the best result when using guanidinium chloride (GuHCl)/urea (1:2) at 80 ◦ C [ 73 ], as shown in Scheme 10. After 30 min, a 95% yield of the desired compound was obtained. A recycling study of the DES showed that the solvent/catalyst can be employed for six reactions without loss in catalytic activity.
Catalysts 2024,14, 120 9 of 16 Catalysts2024,14,xFORPEERREVIEW9of17 Scheme10.Modelreactionforthethree-componentpreparationofapyrroloimidazolebetweenhydantoin,analdehyde,anda1,3-diketonecatalyzedbyGuHCl:U(1:2). In2015,Huetal.describedtheDES-catalyzedsynthesisofasetoffunctionalized pyrrolesthroughamulticomponentreactioninvolvingfourstartingmaterials[74].The pyrrolemoietyispresentinseveralrelevantcompounds,includingAPIs[75].Therefore, severalapproacheshavebeenperformedthroughtheirsynthesis,withMCRsalsobeing described.In2010,apaperdescribedthepreparationofhighlyfunctionalizedpyrrolesby combining1,3-dicarbonylcompounds,amines,aromaticaldehydes,andnitroalkanesin thepresenceofFeCl3asacatalyst[76].Thismethodpresentssomedrawbackssuchaslow yieldsandtediouswork-upprocedures,withothercatalyticmethodologiesbeingdevelopedwithbetteryieldsusingtransitionmetals[77]orothernon-metalliccatalysts[78].All theprocessesdescribedrequiredlongreactiontimes.Therefore,theMCRofaniline,4- chlorobenzaldehyde,acetylacetone,andnitromethanewastestedinthepresenceof ChCl:MA(1:1)asacatalystandsolvent(Scheme11).At80°C,88%ofthefinalpyrrolewas recoveredaftershortreactiontimes.Thereactionwasscaledupto50mmol,observingan 89%yieldafter1h.Thescopeofthereactionwasextendedtootheramines,aldehydes, and1,3-dicarbonylcompounds.Awiderangeofaminescanbeemployed,leadingtomoderate-to-goodyields.Aromaticandheteroaromaticaldehydesalsoaffordedthefinalcompoundswithgoodresults,butunfortunately,alkylaldehydesledtocomplexreactionmixtures.Also,asetof1,3-dicarbonylcompoundsincludingmethyl,ethylor2-methoxyethyl acetoacetate,isobutyl3-oxobutanoate,andmethyl3-oxopentanoatebutylacetatewereanalyzed,achievedhighyields.DESrecyclingforthemodelreactionwasstudied,observing thatthereactioncanbeconductedforfivecycleswithonlyaminimallossintheyield (84%vs.88%). Scheme11.Preparationoffunctionalizedpyrrolesinafour-componentreactioncatalyzedby ChCl:MA(1:1). Thepyranopyrimidinemoietyispresentinawidesetofcompoundspresenting pharmacologicalactivity(antibronchitis,antitumoral,antihypertensive,orantimicrobial) [79,80].Severalmethodologieshavebeendescribedfortheirsynthesis[81,82],butin2023, thesynthesisofcompoundspresentingapyranopyrimidineringoutinamulticomponent reactioncatalyzedbyDESswasdescribed(Scheme12).Thus,aDESformedbymethyltriphenyl-phosphoniumbromide(MTPPBr)and3,4-dihydroxybenzoicacid(protocatechuic Scheme 10. Model reaction for the three-component preparation of a pyrroloimidazole between hydantoin, an aldehyde, and a 1,3-diketone catalyzed by GuHCl:U (1:2). In 2015, Hu et al. described the DES-catalyzed synthesis of a set of functionalized pyrroles through a multicomponent reaction involving four starting materials [ 74 ]. The pyrrole moiety is present in several relevant compounds, including APIs [ 75 ]. Therefore, several approaches have been performed through their synthesis, with MCRs also being described. In 2010, a paper described the preparation of highly functionalized pyrroles by combining 1,3-dicarbonyl compounds, amines, aromatic aldehydes, and nitroalkanes in the presence of FeCl 3 as a catalyst [ 76 ]. This method presents some drawbacks such as low yields and tedious work-up procedures, with other catalytic methodologies being developed with better yields using transition metals [ 77 ] or other non-metallic catalysts [ 78 ]. All the processes described required long reaction times. Therefore, the MCR of aniline, 4-chlorobenzaldehyde, acetylacetone, and nitromethane was tested in the presence of ChCl:MA (1:1) as a catalyst and solvent (Scheme 11). At 80 ◦ C, 88% of the final pyrrole was recovered after short reaction times. The reaction was scaled up to 50 mmol, observing an 89% yield after 1 h. The scope of the reaction was extended to other amines, aldehydes, and 1,3-dicarbonyl compounds. A wide range of amines can be employed, leading to moderate-to-good yields. Aromatic and heteroaromatic aldehydes also afforded the final compounds with good results, but unfortunately, alkyl aldehydes led to complex reaction mixtures. Also, a set of 1,3-dicarbonyl compounds including methyl, ethyl or 2-methoxyethyl acetoacetate, isobutyl 3-oxobutanoate, and methyl 3-oxopentanoate butyl acetate were analyzed, achieved high yields. DES recycling for the model reaction was studied, observing that the reaction can be conducted for five cycles with only a minimal loss in the yield (84% vs. 88%). Catalysts2024,14,xFORPEERREVIEW9of17 Scheme10.Modelreactionforthethree-componentpreparationofapyrroloimidazolebetweenhydantoin,analdehyde,anda1,3-diketonecatalyzedbyGuHCl:U(1:2). In2015,Huetal.describedtheDES-catalyzedsynthesisofasetoffunctionalized pyrrolesthroughamulticomponentreactioninvolvingfourstartingmaterials[74].The pyrrolemoietyispresentinseveralrelevantcompounds,includingAPIs[75].Therefore, severalapproacheshavebeenperformedthroughtheirsynthesis,withMCRsalsobeing described.In2010,apaperdescribedthepreparationofhighlyfunctionalizedpyrrolesby combining1,3-dicarbonylcompounds,amines,aromaticaldehydes,andnitroalkanesin thepresenceofFeCl3asacatalyst[76].Thismethodpresentssomedrawbackssuchaslow yieldsandtediouswork-upprocedures,withothercatalyticmethodologiesbeingdevelopedwithbetteryieldsusingtransitionmetals[77]orothernon-metalliccatalysts[78].All theprocessesdescribedrequiredlongreactiontimes.Therefore,theMCRofaniline,4- chlorobenzaldehyde,acetylacetone,andnitromethanewastestedinthepresenceof ChCl:MA(1:1)asacatalystandsolvent(Scheme11).At80°C,88%ofthefinalpyrrolewas recoveredaftershortreactiontimes.Thereactionwasscaledupto50mmol,observingan 89%yieldafter1h.Thescopeofthereactionwasextendedtootheramines,aldehydes, and1,3-dicarbonylcompounds.Awiderangeofaminescanbeemployed,leadingtomoderate-to-goodyields.Aromaticandheteroaromaticaldehydesalsoaffordedthefinalcompoundswithgoodresults,butunfortunately,alkylaldehydesledtocomplexreactionmixtures.Also,asetof1,3-dicarbonylcompoundsincludingmethyl,ethylor2-methoxyethyl acetoacetate,isobutyl3-oxobutanoate,andmethyl3-oxopentanoatebutylacetatewereanalyzed,achievedhighyields.DESrecyclingforthemodelreactionwasstudied,observing thatthereactioncanbeconductedforfivecycleswithonlyaminimallossintheyield (84%vs.88%). Scheme11.Preparationoffunctionalizedpyrrolesinafour-componentreactioncatalyzedby ChCl:MA(1:1). Thepyranopyrimidinemoietyispresentinawidesetofcompoundspresenting pharmacologicalactivity(antibronchitis,antitumoral,antihypertensive,orantimicrobial) [79,80].Severalmethodologieshavebeendescribedfortheirsynthesis[81,82],butin2023, thesynthesisofcompoundspresentingapyranopyrimidineringoutinamulticomponent reactioncatalyzedbyDESswasdescribed(Scheme12).Thus,aDESformedbymethyltriphenyl-phosphoniumbromide(MTPPBr)and3,4-dihydroxybenzoicacid(protocatechuic Scheme 11. Preparation of functionalized pyrroles in a four-component reaction catalyzed by ChCl:MA (1:1). The pyranopyrimidine moiety is present in a wide set of compounds presenting pharmacological activity (antibronchitis, antitumoral, antihypertensive, or antimicrobial) [ 79 , 80 ]. Several methodologies have been described for their synthesis [ 81 , 82 ], but in 2023, the synthesis of compounds presenting a pyranopyrimidine ring out in a multicomponent reaction catalyzed by DESs was described (Scheme 12). Thus, a DES formed by methyltriphenyl-phosphonium bromide (MTPPBr) and 3,4-dihydroxybenzoic acid (protocatechuic acid, PCAT) at molar ratio 1:1 was employed in the reaction between barbituric acid, 4-hydroxyhydrocoumarin, and
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