Principles of protein targeting to the nucleolus

Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=kncl20 Nucleus ISSN: 1949-1034 (Print) 1949-1042 (Online) Journal homepage: https://www.tandfonline.com/loi/kncl20 Principles of protein targeting to the nucleolus Robert M Martin, Gohar Ter-Avetisyan, Henry D Herce, Anne K Ludwig, Gisela Lättig-Tünnemann & M Cristina Cardoso To cite this article: Robert M Martin, Gohar Ter-Avetisyan, Henry D Herce, Anne K Ludwig, Gisela Lättig-Tünnemann & M Cristina Cardoso (2015) Principles of protein targeting to the nucleolus, Nucleus, 6:4, 314-325, DOI: 10.1080/19491034.2015.1079680 To link to this article: https://doi.org/10.1080/19491034.2015.1079680 © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC© Robert M Martin, Gohar Ter-Avetisyan, Henry D Herce, Anne K Ludwig, Gisela LättigTünnemann, and M Cristina Cardoso View supplementary material Published online: 01 Sep 2015. Submit your article to this journal Article views: 2742 View related articles View Crossmark data Citing articles: 31 View citing articles Principles of protein targeting to the nucleolus Robert M Martin 1 , Gohar Ter-Avetisyan 2 , Henry D Herce 3 , Anne K Ludwig 3 , Gisela L€ attig-T€ unnemann 4 , and M Cristina Cardoso 3, * 1 Instituto de Medicina Molecular; Faculdade de Medicina; Universidade de Lisboa; Lisboa, Portugal; 2 Max Delbrueck Center for Molecular Medicine; Berlin, Germany; 3 Department of Biology; Technische Universit€ at Darmstadt; Darmstadt, Germany; 4 Center for Stroke Research Berlin Charit e; Universit€ atsmedizin Berlin; Campus Mitte; Berlin, Germany Keywords: fluorescence microscopy, GFP, nucleolus, nucleolar localization sequence, protein targeting Abbreviations: aa, amino acid; CPP, cell penetrating peptide; DDC, dense fibrillar compartment; FC, fibrlllar centers; FITC, fluorescein isothiocyanate; GBP, GFP binding protein; GC, granular compartment; GFP, green fluorescent protein; NoLS, nucleolar localization sequence; NLS, nuclear localization sequence; PI, propidium iodide; TAMRA, 5-carboxytetramethylrhodamine; TAT, transactivator of transcription. The nucleolus is the hallmark of nuclear compartmentalization and has been shown to exert multiple roles in cellular metabolism besides its main function as the place of rRNA synthesis and assembly of ribosomes. Nucleolar proteins dynamically localize and accumulate in this nuclear compartment relative to the surrounding nucleoplasm. In this study, we have assessed the molecular requirements that are necessary and sufficient for the localization and accumulation of peptides and proteins inside the nucleoli of living cells. The data showed that positively charged peptide entities composed of arginines alone and with an isoelectric point at and above 12.6 are necessary and sufficient for mediating significant nucleolar accumulation. A threshold of 6 arginines is necessary for peptides to accumulate in nucleoli, but already 4 arginines are sufficient when fused within 15 amino acid residues of a nuclear localization signal of a protein. Using a pH sensitive dye, we found that the nucleolar compartment is particularly acidic when compared to the surrounding nucleoplasm and, hence, provides the ideal electrochemical environment to bind poly-arginine containing proteins. In fact, we found that oligo-arginine peptides and GFP fusions bind RNA in vitro. Consistent with RNA being the main binding partner for arginines in the nucleolus, we found that the same principles apply to cells from insects to man, indicating that this mechanism is highly conserved throughout evolution. Introduction Proteins inside the highly compartmentalized cell nucleus often localize in correlation with their function to discrete subnuclear regions also referred to as subnuclear bodies. 1,2 These nuclear substructures were identified as sites where specific biochemical reactions take place, e.g., DNA replication foci, or as sites of storage and modification for different proteins like in Cajal bodies and nuclear speckles. 3,4 The localization of proteins in the nucleus is often mediated by specialized signals, targeting or interaction motifs of different length and can change over time, cell cycle and with external stimuli. 5-8 The most prominent subnuclear structure is the nucleolus, which appears as a dark region in phase contrast microscopy due to its high density. 9-11 The nucleolus is the place of transcription and storage of the rDNA as well as the assembly and maturation site for ribosomes and, in translationally active cells, filled with pre-ribosomal particles and components. The nucleolar structure is very dynamic and forms around rDNA loci. 12,13 It has further substructures, which are connected to the different steps in ribosome biogenesis and can be best identified by antibody labeling or electron microscopy. The fibrillar centers (FC) harbor the rDNA in the nucleolar center and are surrounded by the dense fibrillar components (DFC) containing the nascent rRNA transcripts. The granular component (GC) is filled with pre ribosomal particles composed of rRNA and proteins, extends into the nucleus and is surrounded by chromatin. 11,14,15 An early study has shown that the nuclear import of several proteins is mediated by short sequences of 6–10 basic amino acid (aa) termed nuclear localization sequences (NLS), some of which also serve as nucleolar targeting sequence (NoLS). 16 Another nucleolar targeting sequence (NoLS) was described in 1990 and contains a minimum of 5 basic aa, often lysines and arginines. 17 The recent systematic analysis of 46 NoLS sequences for their amino acid composition and sequence features has shown greater sequence diversity © Robert M Martin, Gohar Ter-Avetisyan, Henry D Herce, Anne K Ludwig, Gisela L€ attig-T€ unnemann, and M Cristina Cardoso *Correspondence to: M Cristina Cardoso; Email: [email protected] Submitted: 06/24/2015; Revised: 07/29/2015; Accepted: 07/31/2015 http://dx.doi.org/10.1080/19491034.2015.1079680 This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/ by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. 314 Volume 6 Issue 4Nucleus Nucleus 6:4, 314--325; July/August 2015; Published with license by Taylor and Francis Group, LLC RESEARCH PAPER including bipartite sequences as well as overlap with NLS. The study determined also a high content of basic amino acids (48%) and a predominant location in the termini of proteins. 18 Proteins smaller than 60 kDa or up to a diameter of 9 nm can passively diffuse into the nucleus 19 but this process becomes more and more inefficient with increasing size (reviewed in 20,21 ). The nucleus is separated from the cytoplasm by a membrane system and involves an active import mechanism. 22 In contrast, the localization of proteins to the nucleolus has never been shown to involve active transport mechanisms. Nuclear proteins without nucleolar function are often less concentrated or completely excluded from the nucleoli while nucleolar proteins are highly enriched in this nuclear compartment. The localization or exclusion of proteins from the nucleolus is subject to dramatic changes upon cellular stimuli, stress and complete reorganization during cell cycle progression. 23-25 In a previous study, we have shown that a reporter protein traverses nucleoli rapidly with less obstruction compared to nucleoplasmic regions rich in chromatin. 26 In that study, we described a structural feature of the nucleolus with channel like regions void of nucleic acids and proteins that facilitates a fast movement of non-nucleolar proteins through this compartment. Contrastingly, the neighboring densely packed structures in the nucleolus reduce the available space for the localization of non-nucleolar proteins. 26 Still it is unclear, what are the essential properties or requirements of proteins and peptides to accumulate in the nucleoli and what are the features of these molecules that prevent nucleolar accumulation. The systematic identification of NoLS sequences by an artificial neural network trained with confirmed NoLS sequences has found thousands of potential sequences that could serve to mediate nucleolar targeting. Several previously unknown NoLS have been experimentally confirmed thereafter. 18,27 However, a considerably false positive prediction rate especially among sequences from nuclear proteins raises the question of how NLS and NoLS can be distinguished. The difference in the composition of a standard NLS and the peptides that accumulate in nucleoli is rather subtle, with the NLS being a few basic charged amino acids shorter than, e.g., the nucleolar marker peptide of 10 arginines described by us. 16,28 This observation led to the following 2 questions: (a) what are the specific requirements in amino acid composition for peptides and proteins to localize to the nucleoli and (b) what are the key differences between NoLS and NLS sequences? In this study, we systematically investigated the molecular properties of peptides and proteins that result in different distribution levels between nucleoplasm and nucleolus. For this purpose, we tested and analyzed the distribution of a series of fluorescently labeled short peptides with different charge and isoelectric properties between the cytoplasm, nucleoplasm and their accumulation in the nucleolus. The same peptide sequences were genetically fused to the 50end of the open reading frame coding for the tracer protein GFP, with no intracellular binding sites, to test the effect of the same short peptide sequences on the localization of proteins. Materials and Methods Peptides, plasmids and cell lines All peptides were synthesized from L-amino acids except decaarginine (R10), which was made as 2 versions one from Land one from D-amino acids by Peptide Specialty Laboratories GmbH (Heidelberg, Germany). The peptides were coupled directly to fluorescein at the N-terminus, contain a C-terminal amide-group and were purified by HPLC. The TAT peptide was synthetized with D-amino acids and conjugated with TAMRA as described. 29 It can be excluded that different amounts of fluorescent labeling on the different peptides play a role in the intracellular distribution, since the in vitro synthesis of peptides coupled to a resin material allows only in line addition of amino acids and the fluorophore. The pI values of the peptides and proteins were calculated using the ProtParam tool on http://web.expasy. org/protparam/. The GFP based mammalian expression constructs were generated by using oligonucleotides encoding the respective poly-D, poly-G and poly-R sequences (supplementary Table 1). The oligos with overhanging 30and 50AgeI sites were annealed and ligated into the AgeI site of an NLS-GFP construct based on the pEVRF vector. 30 This vector is identical to the one described before for the CMV-driven expression of GFP-tagged PCNA. 31 In addition, the pEGFP-C1 vector (Clontech, Heidelberg, Germany) without a NLS sequence was used. Human HeLa and HEK 293-EBNA cells, as well as mouse C2C12 myoblasts were cultured as described before. 32,33 Pac2 zebrafish cells were grown at 28C in Leibovitz L-15 medium supplemented with 15% fetal calf serum in presence of 1% penicillin-streptomycin. Sf9 insect cells were maintained in EXCELL TM 420 Insect Serum Free (SAFC) medium with L-glutamine (Biosciences) and 10% fetal calf serum and cultured with shaking at 100 rpm and 28C. The L40ccua yeast strain used for the current study has the following genetic background: (MATa his3_200 trp1–901 leu2–3,112 LYS2::(lexAop)4-HIS3 ura3:: (lexAop)8lacZ ADE2::(lexAop)8-URA3 GAL4 gal80 can1 cyh2). 34 Yeast cells were grown at 30C in yeast extract/peptone/ dextrose (YPD) and were supplemented with 2% glucose as a carbon source. Live cell peptide loading and transfections All peptide experiments were performed exclusively with living cells plated on 8-well Ibidi chambers. The different polylysine (poly-K) and poly-arginine (poly-R) peptides were scratch loaded into living cells. For the scratch loading of peptides we used syringes and scratched over the chamber bottom in different directions. 35,36 The size of the peptides permitted passive diffusion into the cells and nuclei after delivery. After the scratch loading the cells were allowed to recover for about half an hour at 37C before exchanging the medium to remove unloaded peptides. Microscopy and image acquisition was started immediately after the procedures described above to circumvent peptide shortening by proteases and, hence, mislocalization. Peptide concentrations from 10 to 100 mm were initially tested and no apparent changes in cell viability were observed during the course of the www.tandfonline.com 315Nucleus experiments. As low concentrations of 20 mM were sufficient for quantitative imaging, these conditions were used for the subsequent quantification. Scratch loaded cells did not show dramatic differences in the level of intracellular fluorescence, likely because the peptide concentration in the medium was the same in all cases. Only cells with normal cellular physiology comparable to non-scratch loaded cells were imaged. The criteria for the selection of cells that repaired after scratch loading were: i) cells are completely attached and stretched out on the chamber bottom surface; ii) no membrane lesions or leakage of cytoplasmic constituents visible in phase contrast; iii) round or oval shaped nuclei with smooth outline and nucleoli visible in phase contrast. The transfection of cells with plasmid constructs was performed using the CaPO 4 -DNA co-precipitation protocol as described before, 37 except for HEK 293-EBNA cells, which were transfected using poly-ethylenimine (1 mg/mL in ddH 2 O, pH 7; Sigma-Aldrich, St. Louis, MO, USA) as described before. 38 Cell fixation and antibody staining Cells grown on glass coverslips for 24 h were incubated with 10 mM FITC-R10 for 1 h. After washing with 1xPBS, cells were fixed in 3.7% formaldehyde in PBS for 10 min and permeabilized with 0.25% Triton X100 in PBS for 10 min. Immunostaining with anti-B23 mouse monoclonal antibody (clone FC82291; Sigma) was followed by detection with donkey anti-mouse IgG antibody conjugated with TexasRed (Jackson). Coverslips were mounted with Moviol (Sigma). Microscopy, image acquisition and analysis Live cell microscopy was performed with a Zeiss LSM510Meta confocal setup mounted on an Axiovert 200 M inverted microscope using a 63x phase contrast plan-apochromat oil objective NA1.4. The microscope was placed in an incubation chamber heated to 37C to maintain the cell incubation conditions (Okolab, Italy). For the measurements of plasmid transfected cells, a Leica TCS SP confocal microscope was used. For all acquisition settings the main beam splitter was HFT UV/488/ 543/633. FITC was excited with the 488 nm line of an argon laser and fluorescence detected with a band-pass filter 500– 530 nm. Phase contrast or differential interference contrast images were recorded simultaneously with FITC fluorescence in the transmission channel. The live-cell DNA dye DRAQ5 was used and imaged as described. 32 Cells loaded with peptides were chosen for imaging by having low but easily detectable intracellular fluorescence intensity. The same criteria were applied to cells transfected with plasmid constructs. Imaging of live cells was performed using as much as possible similar settings per instrument. The SNARF 4F cell-permeant dye (Sigma) was loaded into cells by incubating for 30 min at a final concentration of 5 mM in growth medium. Fluorescence was excited at 561 nm and emission detected at 587 nm and 640 nm. For image analysis, the nuclei were identified in the corresponding phase contrast images by means of the nuclear membrane delineation and nucleoli correspond to the dark dense structures inside the cell nucleus. Identification of nucleoli was confirmed in earlier experiments using a nucleolar marker for living cells. 28 The selection of regions (cytoplasm, nucleus, nucleolus) and measurements of mean signal intensity were performed in ImageJ. Measurements of mean fluorescence intensities were performed by excluding non-interest areas from the measurement, e.g. the nucleoli areas excluded from nucleoplasm and, correspondingly, for all other regions intensities were determined. In each experiment the average was calculated for 10 cells for each peptide and protein. Data were normalized to 1.0 (100%) with respect to the nucleoplasmic fluorescence (excluding the nucleoli) to directly illustrate the relative accumulation of fluorescence in the nucleoli. Cytoplasmic fluorescence levels were normalized correspondingly (excluding nucleus and nucleoli). Data analysis and statistical tests were performed and displayed with Origin 7 software. The complete list of predicted nucleolar localization sequences was downloaded from http://www.compbio.dundee.ac.uk/wwwnod/ and analyzed for mean length, mean numbers of amino acids and frequencies in Excel and Origin 7. Protein preparation GFP and NLS-R7-GFP were prepared from HEK cells 48 h post transfection using an ice-cold buffer composed of 20 mM Tris-HCl, 1.5 mM MgCl2, 0.2 mM EDTA, 1 M NaCl and 0.4% NP40. After homogenization by mechanical shearing through a 23 gauge syringe, lysates were incubated for 10 min on ice. Following centrifugation (14,000 rpm for 12 min at 4C) supernatants were incubated for one hour with GFP binding protein (GBP) coupled to sepharose beads at 4C on a rotary shaker as described. 39 GBP bound proteins were washed 3 times with ice-cold HEPES buffer (140 mM NaCl, 2.5 mM KCl, 5 mM HEPES, 5 mM glycine, pH 7.4) and used for the in vitro RNA pull-down assay. All buffers were supplemented with 1 mM of protease inhibitor PMSF (Carl Roth, Karlsruhe, Germany). RNA preparation Total RNA was isolated from HEK cells using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. To remove traces of genomic DNA, RNA was treated with RNase-free, recombinant DNaseI (Macherey Nagel, Dueren, Germany) for 30 min at 37Cand further purified with the Qiagen RNeasy Mini Kit. To assess the concentration and purity of RNA, the ratio of absorbance at 260 nm and 280 nm was measured on a TECAN infinite M200 plate reader (Tecan Group Ltd., Maennedorf, Switzerland). To further verify RNA quality, total RNA was denatured for 5 minutes at 99C, separated on a 1.5% Tris-acetate-EDTA/agarose gel supplemented with 0.05 mL/mL Roti-Safe GelStain (Carl Roth, Karlsruhe, Germany) by electrophoresis and imaged on an Amersham Imager 600 (GE Healthcare, Freiburg, Germany). In vitro RNA binding assays For RNA slot blots, total RNA was mixed with RNase-free ddH 2 O at a final concentration of 500 ng/mL and blotted on pre-equilibrated (5 min methanol, 5 min 20x saline sodium 316 Volume 6 Issue 4Nucleus citrate) PVDF membranes (0.45 mm pore size; Pall GmbH, Dreieich, Germany). After air-drying, membranes were blocked for 30 min with 5% milk in PBS supplemented with 1x ProtectRNA RNase inhibitor (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 1 h with either TAT, D-R10, or L-R10 (all fluorescently labeled), respectively, on a rotary shaker. Following three washing steps (5 min each) with HEPES buffer, remaining fluorescent signals were detected on an Amersham Imager 600 (GE Healthcare, Freiburg, Germany). Quantities of blotted RNA were monitored in parallel by methylene blue (0.02%) staining (Carl Roth, Karlsruhe, Germany) in 0.3 M sodium acetate (Merck, Darmstadt, Germany) pH 5.5. For the pulldown assay, immobilized GFP and NLS-R7-GFP, respectively, were incubated with equal amounts (3.4 mg) of total RNA in 20 mL HEPES buffer for 1.5 h at 4C on a rotary shaker. After three washing steps with HEPES buffer, RNA was labeled with 3.3 mg/mL propidium iodide (Sigma-Aldrich, St. Louis, MO, USA). Following three washing steps in HEPES buffer, remaining fluorescent signals were measured on a TECAN infinite M200 plate reader (Tecan Group Ltd., Maennedorf, Switzerland) using excitation/emission at 488/555 nm and 515/617 nm for GFP and propidium iodide, respectively. To control for the amount of GFP and NLS-R7-GFP, fluorescent signals of propidium iodide were normalized to GFP signals. In addition, the same beads were imaged in an UltraVIEW VoX spinning disc confocal system (PerkinElmer), mounted on a Nikon TI microscope. Images were taken with a 20x/0.7 NA objective. GFP and propidium iodide or TAMRA were imaged with 488 and 561 nm laser excitation and 527 §55 and 612 § 70 nm emission filters, respectively. Results Short specific sequence motifs in proteins are well known to play a key role in localizing proteins to specific cellular and subcellular compartments. We have shown earlier that short peptides of 10 arginines with a strong basic charge have the potential to accumulate inside the nucleoli of living cells and can be used to label this subnuclear compartment. 28 Now, we wished to investigate the specific molecular requirements of peptides and proteins that are necessary and sufficient to reach and accumulate inside of the nucleolus. Therefore, our first aim was to determine the properties of charge, length and composition of basic charged peptides necessary for nucleolar targeting and accumulation. We used short synthetic peptides labeled with FITC for visualization, which were scratch loaded into living C2C12 mouse myoblast cells. The peptides, either composed of arginines (R) or lysines (K) with a length varying from 5 to 12 amino acids, were allowed to enter the cells and distribute in the cytoplasm and nucleus. The observation of the cells by confocal microscopy started after a recovery time of 30 min after scratching loading. For each peptide at least 10 cells were selected and imaged with respect to similar intracellular fluorescence intensity levels. The microscopic observation of the cells shows for all peptides a homogeneous distribution in the cytoplasm, where only vesicular structures were free of labeled peptides. Similarly, in the nucleoplasm the peptides distributed without enriching at any foci. All L-amino acid peptides tested here, K5 to K12 and R5 to R12 accumulated predominantly in the nucleus compared to the cytoplasm, as shown by the fluorescence intensity in mouse C2C12 cells (Fig. 1A and B). The peptides R6 to R12 clearly showed higher fluorescence intensity in areas inside the cell nucleus, which were identified as nucleoli correlating with the dark dense structures visible in the phase contrast images (Fig. 1A). The peptide R5 did not accumulate in nucleoli (Fig. 1A), which was also the case for all poly-K peptides (Fig. 1B). This indicates that charge alone is not the only determinant but rather the isoelectric properties, which differ between K and R polymers. To quantitatively analyze the intensity and distribution patterns, we determined for 10 cells the mean fluorescence intensity in the cytoplasm, nucleus (excluding nucleoli) and in the nucleoli. The regions for fluorescence intensity measurements were chosen with reference to the phase contrast images and overlayed with the fluorescence image as shown in Figure 1C. Data were normalized to the nucleoplasmic levels to reflect the accumulation potential of molecules in the nucleolus over the nucleoplasmic levels (Fig. 1C). The numerical values are given in supplementary Tables 2 and 3. The comparison of the mean fluorescence intensity shows, for all poly-K peptides, an average cytoplasmic level of 0.74 fold less compared to the nucleoplasm. For poly-R peptides the cytoplasmic level is on average 0.68 fold less than in the nucleoplasm (Fig. 1A, B and supplementary Table 2). These data already demonstrate that poly-R peptides have the potential for stronger accumulation in the nucleus than poly-K peptides. Although all peptides tested are small enough to passively diffuse in and out of the nucleus and should distribute evenly, all of them accumulate in the nucleoplasm. Strikingly, only the peptides R6 to R12 show a further visible accumulation inside nucleoli with an average of 1.16-fold over the nucleoplasmic level (Fig. 1C). The statistical analysis with a non-parametric Kruskal-Wallis ANOVA test shows significance for the accumulation of R6 to R12 in the nucleolus. The poly-K peptides show only slightly higher level in the nucleoli with values between 1.02 and 1.06 fold over the nucleoplasm. An increase of the peptide length from R6 to R12 and thus the positive charge and isoelectric properties does not linearly increase the nucleolar accumulation level of the peptides. The highest level of nucleolar accumulation was found for the R9 peptide with 1.22 fold over the nucleoplasmic level, while the mean accumulation of R11 and R12 rather decreases slightly to 1.12 and 1.14-fold respectively (Fig. 1A, B and supplementary Table 2). This suggests that there is a saturation/plateau level at and above 9 arginine residues. From yeast to human cells, main features of the nucleolus structure and function are conserved as it forms around clusters of rRNA genes and is the sites of ribosome biogenesis. We were now interested if the nucleolar localization and accumulation of proteins and peptides is similarly conserved. Therefore, we tested the nucleolar accumulation potential of the R10 peptide in cultured cells of different species. We scratch www.tandfonline.com 317Nucleus loaded the FITC labeled R10 into cells from yeast (S. cerevisiae), insects (D. melanogaster), fish (D. rerio), mouse (M.musculus)andhuman(H. sapiens) cells and analyzed the localization of the peptides by confocal microscopy (Fig. 1D). In yeast cells the nucleus and nucleolus are more difficult to identify and clear accumulations inthecellsarenotasvisibleas forthecellsofhighereukaryotes. Regions with less peptide inside the yeast cells could correspond to the vacuole. In the cells from insect, fish, mouse and human, on the other hand, the nucleoli are visible in the phase contrast images and show clearly an accumulation of the R10 peptide compared to the nucleus and cytoplasm of the cells (Fig. 1D). To clarify the localization of the FITC-R10 in yeast we performed a co-labeling of yeast cells with the live cell DNA dye DRAQ5. 32 In this experiment the yeast nucleus is visible as intense labeled round compartment in the cells. In some cells we could identify a crescent shaped region in the periphery of the nucleus with higher FITC-R10 intensity compared to the nuclear interior and the cytoplasm. A corresponding fluorescence intensity linescan analysis confirms an accumulation of the peptide in a crescent shaped region in the nuclear periphery (Fig. 1E). Early studies on the composition of yeast nuclei have shown that, depending on the orientation a round or crescent shaped peripheral nuclear compartment harbors the nucleolus. 40-42 Allcelltypesofmulticellular organisms tested here show a similar enrichment of the labeled poly-R peptides in the nucleoli. Some yeast cells show a similar accumulation in the nuclear periphery. The accumulation of peptides like R10 inside nucleoli is, thus, evolutionary conserved at least from insects to humans. Notably, HeLa cells in metaphase having no cell nucleus and with the nucleoli disassembled lack also the nucleolar accumulation of peptides found in interphase cells. Thus, the accumulation of Figure 1. Distribution of poly-(R)and poly-(K)peptides in living cells. Intracellular distribution of poly-R peptides in (A) and poly-K peptides (B) in living C2C12 mouse cells. In each panel, the fluorescence image is on top of the corresponding phase contrast image. Nucleoli are clearly visible as dark round structures within the nuclei in the phase contrast images. The bar diagrams in (C) show the quantification of the poly-K and poly-R peptide mean fluorescence in cytoplasm, nucleoplasm and nucleoli averaged for 10 cells from 2 independent experiments. The nucleoplasmic values were used for normalization. Areas for quantification were defined as described in methods and overlayed with fluorescence images as shown. Intracellular distribution of R10 peptide in living cells of different species as indicated is shown in (D). In (E) living yeast cells were further stained with DRAQ5 for better visualization of the nucleus and a line intensity profile in arbitrary units (a.u.) of both DNA and peptide is shown. The intracellular distribution of (D) and L-R10 peptides in living C2C12 cells and the corresponding quantification of mean fluorescence intensities is shown in (F). Scalebars: 5 mm. 318 Volume 6 Issue 4Nucleus poly-R peptides is directly linked to the structure and function of the nucleolus (supplementary Fig. 1D). All amino acids incorporated into proteins by translation in living organisms are L-enantiomers and only post-translational enzymatic reactions in various organisms convert some to Damino acids. 43,44 Besides that, free D-amino acids were discovered only in brain tissue. 45 We argue that a poly D-arginine peptide may not exhibit the specific binding to the same cellular target components, e.g., proteins or nucleic acids, as the L-enantiomer. In contrast, the peptide charge and isoelectric properties are not altered when composed of D-amino acids. This phenomenon is exploited in mirror image phage displays used in the search for therapeutic peptides with novel targets and improved properties. 46,47 The latter exploits the stability of the D-amino acid peptides to proteolytic degradation. Hence, we compared the distribution of FITC-R10 composed of D and L amino acids (Fig. 1F). Inside living cells both Dand L-R10 peptides distribute homogenously in the cytoplasm and accumulated in the nucleoli. The main difference in the distribution is that D-R10 had a much lower concentration in the nucleoplasm compared to the L-R10, therefore the measurement of relative accumulation of D-R10 over the nucleoplasmic level appears much stronger (Fig. 1F). However, the absolute detected fluorescence intensities were comparable between both types of peptides using the same microscopic detection settings. This finding indicates either a binding of the L-peptide to a nucleoplasmic component, which is not the case for the D-peptide, and/or the lack of degradation of the D-R10. Interestingly though, the mean intensity of fluorescence in the nucleoli is at similar levels for both types of peptides. Next, we tested the role of various peptides in the targeting of proteins inside the cell nucleus. We used peptides fused to the neutral tracer protein GFP to determine localization patterns. For this purpose, we used GFP already fused to a NLS sequence (SV40 T antigen derived; PKKKRKV 48 ) and added downstream further basic, acidic and neutral peptides of different lengths followed by the enhanced GFP coding sequence. The different GFP fusions were expressed in C2C12 mouse myoblast cells (Fig. 2A) and in human HeLa cells (supplementary Fig. 1) and imaged with a confocal microscope. We also tested the distribution of an uncoupled GFP and the NLS-GFP alone as comparison and as controls for cytoplasmic and nucleoplasmic localization with no expected nucleolar localization and accumulation. The microscopic images of the GFP versions in Figure 2A show for the GFP without NLS a homogeneous distribution throughout the cell with similar levels in cytoplasm and nucleus with significant reduction in the nucleoli (p D0.01 see supplementary Table 2). The slightly lower mean intensity in the cytoplasm is the result of membranous compartments devoid of GFP protein (Fig. 2A and C; supplementary Table 2). The homogeneous distribution between nucleus and cytoplasm is the result of passive diffusion into the nucleus. Molecules with adiameterofupto»9nmare capable of entering the cell nucleus by passive diffusion, which has been measured for fluorescent molecules the size of GFP to occur within a few minutes. 19,49 The NLS coupled GFP, though, shows more than 2-fold accumulation in the nucleus over the cytoplasmic level but with a similar reduction in the nucleoli, visible as round elevated objects in the DIC images. This distribution pattern is similar for all NLS-GFP variants with additional acidic (aspartate) and neutral (glycine) amino acid series (Fig. 2A and C). The nucleolar level of the different proteins in Figure 2A is significantly reduced in the range of 0.7 to 0.5-fold compared to the nucleoplasmic level as determined by measuring the mean fluorescence intensity (Fig. 2C and supplementary Table 2). Figure 2. Intracellular distribution of peptide tagged GFP tracer proteins in living cells. (A) Representative microscopic images of live C2C12 cells transfected with GFP, NLS-GFP and a fusion of NLS-GFP with 8 glycines (8G) as example for the addition of neutral amino acids is shown in the first panel. The second panel shows images of cells with fusions of an increasing number of aspartates as examples for acidic amino acid fusions to NLS-GFP. The GFP fluorescence is depicted in the upper row with the corresponding differential interference contrast (DIC) images below. (B) Microscopic Images of the distribution of the NLS-GFP fused to 4 to 7 additional arginines. The graphs in (C) represent the mean distribution of the GFP constructs depicted in (A) and (B) in cytoplasm and nucleoli in relation to the nucleoplasm for an average of 10 cells from 2 independent experiments. Scalebars: 5 mm. www.tandfonline.com 319Nucleus Contrastingly, all NLS-GFP versions coupled to poly-R peptides from R4 to R7 show a clear accumulation in the nucleoli around 2 to 3-fold over the nucleoplasmic level. The nucleolar accumulation is lowest for NLS-R4-GFP at 1.7-fold and increases with the growing number of arginine residues up to 2.7-fold for NLS-R7-GFP (Fig. 2B and C and supplementary Table 2). Interestingly, the comparison of the data for peptides and proteins shows that in contrast to the FITC-R5 peptide, we see already for the R4 fused to NLS-GFP a nucleolar accumulation. This may reflect the proteolytic degradation of the L-amino acids containing peptides versus the more stable corresponding GFP fusion proteins. On the other hand, the additional arginine within the NLS sequence could account for this apparent difference. Noteworthy, peptides without nucleolar accumulation like K5 and R5 show no reduced concentration inside nucleoli. Already in an earlier study testing functional peptides not accumulating in nucleoli, we observed a similar homogenous nuclear and nucleolar distribution of a fluorophore labeled peptide. 50 These observations exemplify the dense structure of the nucleoli resulting in a size dependent sieving effect for molecules without potential for accumulation therein. Larger molecules like GFP (with dimensions of around 2.4 £4.2 nm) 51 are significantly excluded compared to much smaller peptides. Interestingly, the R5 to R7 coupled NLS-GFP molecules show in addition an even higher efficiency of nuclear import with levels up to 10-fold over the cytoplasm compared to NLS-GFP alone (Fig. 2B, C and supplementary Table 2). This observation shows that the addition of arginine residues to a minimal NLS increases the efficiency of nuclear import largely exceeding the passive leakage efflux of small protein molecules through nuclear pores. Alternatively, their active retention in the nucleus by binding, e.g., to the nucleolus, prevents their exit from the nucleus. The data presented here so far demonstrate that peptides and proteins with a strong positive charge and high isoelectric point have the potential to accumulate in the nucleolus of live cells. The threshold for strong nucleolar accumulation in peptides is R6, while in proteins bearing an NLS (with one additional R) already 4 additional consecutive arginines are sufficient. In addition, the nuclear import is enhanced by the addition of at least 5 arginine residues in proximity to a minimal NLS. We conclude that the charge and isoelectric properties of the peptides and protein domains responsible for nucleolar accumulation is key in this process because first, the difference between nuclear import and nucleolar accumulation has never been shown to rely on an active mechanism. Second, even peptides synthesized with D-amino acids show nucleolar accumulation, which may fail to specifically bind to the same cellular targets as their L-enantiomer counterparts. The evidence for a charge and isoelectric point dependency for peptide and protein accumulation in nucleoli led us to postulate the presence of an electrochemical component. Electrochemical interactions that would accumulate positively charged basic peptides should be mediated by highly abundant or strong negatively charged acidic nucleolar components. These have to be confined to nucleoli as are the accumulated basic peptides and proteins. Such molecules could be nucleic acids, which are highly abundant in the nucleoli in form of rRNA. Therefore, we wanted to determine the pH distribution inside the nucleus in living cells. We used the live cell permeable fluorescent dye SNARF-4F, which has 2 pH dependent ratiometric emission peaks at around 587 and 640 nm. The calculation of the ratio of the fluorescence intensity at the 2 emission peaks gives information on the pH of subcellular structures. The ratiometric images of HeLa cells show in the fluorescence channel view as well as in the intensity ratio view different cellular structures highlighted (Fig. 3A top row). The magnified image detail shows structures at the nuclear periphery and in the center of the nucleus highlighted as more acidic (Fig. 3A bottom row). Although the cytoplasm is generally considered to have a neutral pH, the more acidic regions in the cytoplasm do most likely represent parts of the Golgi network and organelles like peroxisomes. 52 The central nuclear region with more acidic pH is reminiscent of the nucleoli in HeLa cells shown in the phase contrast images in Figure 1D as well as the DIC images in Figure 2 and in supplementary Figure 1. Almost Figure 3. Intracellular pH landscape by ratiometric fluorescence microscopy toward the generation of an intracellular pH landscape. The figure in (A) displays fluorescence microscopy images of the pH sensitive dye SNARF-4F in live HeLa cells (color). The green channel shows the emission at 587 and the red channel the emission of the dye at 640 nm during excitation with the same wavelength. The resulting intensity ratio image (gray scale) shows ratiometric differences between the intensities of the 2 fluorescence emission peaks of the dye due to pH variations in subcellular structures. The fluorescence color scale and the ratio scale in gray indicate the relative range from more acidic to more basic pH. Panel (B) illustrates a map of the intracellular pH landscape by measuring the pH in various cellular compartments and substructures like nucleoplasm and nucleolus (modified from 53 ). Scalebars: 5 mm. 320 Volume 6 Issue 4Nucleus every cell in the overview image has the nucleoli highlighted as more acidic compared to the nucleoplasmic surrounding (Fig. 3A top row). Although precise measurements of subcellular pH values are not possible with the SNARF-4F dye, it allows us to conclude that the nucleolar environment is more acidic than the neutral pH of the nucleoplasm. The subcellular pH landscape was measured in various studies before and is reviewed in. 53 Figure 3B summarizes the reviewed data and was adapted to now incorporate the differentiated neutral pH of the nucleoplasm and more acidic pH of the nucleolus obtained from our measurements. Next, we wanted to test whether the peptides and proteins with the sequence requirements sufficient to accumulate in nucleoli do bind RNA. To test our hypothesis, we purified total RNA (Fig. 4A) and performed slot blot analysis (Fig. 4B). We blotted increasing amounts of total RNA on a PVDF membrane followed by incubation with either fluorescently labeled L-R10, or D-R10, respectively (Fig. 4B). The fluorescently tagged Dand L-R10 were only detected on slots spotted with RNA (Fig. 4B, rows 4 and 6) but not in control slots without RNA (Fig. 4B, rows 3 and 5). As a loading control, membranes were stained in parallel with methylene blue (Fig. 4B, rows 1 and 2). To test the RNA binding of basic peptides fused to GFP, we performed an in vitro RNA pulldown assay using NLS-R7GFP immobilized to sepharose beads via the GFP binding protein (GBP) 39 (Fig. 4C, scheme). This assay allowed us to observe (Fig. 4C, confocal image) and quantify (Fig. 4C, bar plot), the binding of RNA to NLS-R7GFP. The accumulation of peptides in nucleoli is a common feature of arginine-rich cell penetrating peptides (CPPs). 54 Consistently, we found that the TAT peptide, one of the best known CPPs derived from HIV1 TAT protein, 55 also binds to RNA (supplementary Fig. 2). Defining experimentally the requirements of nucleolar peptide and protein accumulation has shown that several arginines in sequence are required. At least 6 arginine residues in a peptide and 4 arginines in combination with an NLS provide sufficient localized surface charge for nucleolar accumulation. Importantly, charge alone cannot explain the difference between poly-R and poly-K, which may additionally rely on the higher number of functional amino groups in arginines vs. lysines. Now we asked, how our findings relate to NoLS sequences found in the human proteome? A previous comprehensive study of nucleolar targeting sequences has systematically evaluated 46 nucleolar targeting sequences curated from the literature. Based on this analysis an algorithm was developed to predict nucleolar targeting based on peptide amino acid composition. The algorithm was then run against the International Protein Index database (IPI 3.4) and predicted >10,000 putative nucleolar targeting sequences. We have used this list to perform further analysis of the amino acid composition of predicted nucleolar targeting sequences. An overall statistics shows that the predicted NoLS have an average length of 27 amino acids with a most frequent length of 20 amino acids. The vast majority (83%) of predicted NoLS sequences has a length of 30 amino acids or less. For these NoLS the mean ratio of the number of basic (lysine, arginine and histidine) versus the total number of amino acids is 0.34. The shortest and most frequently predicted NoLS sequences of 20 amino acids therefore contain on average at least 7 basic amino acids. The 15 Figure 4. Analyses of RNA binding to poly-(R)peptides and GFP fusions. (A) Total RNA used for in vitro RNA binding assays separated by size showing the absence of genomic DNA contamination, as well as characteristic bands for the 28S and 18S rRNA, which are primarily synthesized and localized in nucleoli. The slot blot in (B) (representative from 2 independent experiments), rows 1 and 2, show a methylene blue stained PDVF membrane in the absence (1) and presence (2) of different amounts of total RNA (0.5 mg and 1 mg) and were used as a loading control. The rest of the blot shows the binding of fluorescently tagged L-R10 (3 and 4) and D-R10 (5 and 6) in the absence (3 and 5) and presence (4 and 6) of total RNA. Slots lacking RNA (rows 5 and 6) do not show binding of Land D-R10, respectively. In contrast, slots probed with increasing amounts of RNA show increasing Land D-R10 binding. (C)In vitro RNA pulldown assay using NLS-R7-GFP immobilized to sepharose beads via the GFP binding protein (GBP) (scheme). RNA was stained using propidium iodide (PI) and GFP alone was used as a negative control. RNA binding was measured on a fluorescent plate reader (bar plot) and imaged by confocal microscopy (microscopic images). Plots represent the average plus standard deviation of 2 independent experiments. Scalebar: 100 mm. www.tandfonline.com 321Nucleus