A Psl-like polysaccharide has a key role in the biofilm architecture of two plant-associated Pseudomonas
Heredia Ponce, Zaira María,Purtschert, G.,Gutiérrez Barranquero, José Antonio,Eberl, Leo,De-Vicente-Moreno, Antonio,Cazorla-López, Francisco Manuel
- Published
- 2019
- Publisher
- IS-MPMI
- Language
- en
Abstract
The Psl polysaccharide has only been studied in Pseudomonas aeruginosa, and its role in bacteria that interact with plants is still unknown. On the one hand, P. syringae is a model of study of plant-pathogen interactions. On the other hand, P. chlororaphis is a biocontrol agent of plant-fungal diseases, as the white root rot caused by Rosellinia necatrix. In silico analysis have let us identify in both species a genomic region which is ortholog to the Psl-encoding region of P. aeruginosa PAO1. We constructed mutants of this region in both strains and analysed its phenotype in biofilm formation, both in static microwell plates and dynamic flow-cell chamber experiments. The results revealed a different and an important role of this polysaccharide in the biofilm architecture of both strains. Furthermore, in P. syringae this Psl-like polysaccharide has a very important role in swarming motility. All these striking phenotypes led us further study the implication of this polysaccharide in the lifestyles of both models of plant-bacteria interaction.
Full text
A Psl-like polysaccha ide has a key ole in he bio ilm a chi ec u e o wo plan -
associa ed Pseudomonas
He edia-Ponce, ZM; Pu sche , G; Gu ié ez-Ba anque o, JA; G; Ebe l, L.; de Vicen e,
A; Cazo la, FM
The Psl polysaccha ide has only been s udied in Pseudomonas ae uginosa, and i s ole
in bac e ia ha in e ac wi h plan s is s ill unknown. On he one hand, P. sy ingae is a
model o s udy o plan -pa hogen in e ac ions. On he o he hand, P. chlo o aphis is a
biocon ol agen o plan - ungal diseases, as he whi e oo o caused by Rosellinia
neca ix. In silico analysis ha e le us iden i y in bo h species a genomic egion which is
o holog o he Psl-encoding egion o P. ae uginosa PAO1. We cons uc ed mu an s o
his egion in bo h s ains and analysed i s pheno ype in bio ilm o ma ion, bo h in s a ic
mic owell pla es and dynamic low-cell chambe expe imen s. The esul s e ealed a
di e en and an impo an ole o his polysaccha ide in he bio ilm a chi ec u e o bo h
s ains. Fu he mo e, in P. sy ingae his Psl-like polysaccha ide has a e y impo an ole
in swa ming mo ili y. All hese s iking pheno ypes led us u he s udy he implica ion o
his polysaccha ide in he li es yles o bo h models o plan -bac e ia in e ac ion.