The Compound 2-Hexyl, 5-Propyl Resorcinol has a Key Role in Biofilm Formation by the Biocontrol Rhizobacterium Pseudomonas chlororaphis PCL1606
Tienda Serrano, Sandra,Heredia Ponce, Zaira María,Arrebola-Díez, Eva María,Calderón, Claudia E.,Cárcamo-Oyarce, Gerardo,Eberl, Leo,De-Vicente-Moreno, Antonio,Cazorla-López, Francisco Manuel
- Published
- 2019
- Publisher
- IS-MPMI
- Language
- en
Abstract
The production of the compound 2-hexyl-5-propyl resorcinol (HPR) by the rhizobacterium Pseudomonas chlororaphis PCL1606 (PcPCL1606) is crucial for fungal antagonism and biocontrol activity against the phytopathogenic fungis. The production of HPR is also involved in plant root colonization. This pleiotrophic response prompted us to study the potential role of HPR production in biofilm formation. The swimming motility of PcPCL1606 is enhanced by the disruption of HPR production. Mutants deficient in HPR production, revealed that colony morphology, adhesion and typical air–liquid interphase pellicles were all dependent on HPR production. The role of HPR production in biofilm architecture was analyzed at the initial stage of biofilm maturation using flow-through flow cells chambers . These experiments revealed that the HPR mutant cells had less tight unions than those producing HPR, suggesting an involvement of HPR in the production of the biofilm matrix by PcPCL1606.
Full text
The Compound 2-Hexyl, 5-P opyl Reso cinol has a Key Role in Bio ilm Fo ma ion
by he Biocon ol Rhizobac e ium Pseudomonas chlo o aphis PCL1606
Sand a Tienda, Zai a He edia-Ponce, E a A ebola, Claudia E. Calde ón, Ge a do
Cá camo-Oya ce , Leo Ebe l, An onio De Vicen e and F ancisco M. Cazo la
The p oduc ion o he compound 2-hexyl-5-p opyl eso cinol (HPR) by he
hizobac e ium Pseudomonas chlo o aphis PCL1606 (PcPCL1606) is c ucial o ungal
an agonism and biocon ol ac i i y agains he phy opa hogenic ungis. The p oduc ion
o HPR is also in ol ed in plan oo coloniza ion. This pleio ophic esponse p omp ed
us o s udy he po en ial ole o HPR p oduc ion in bio ilm o ma ion. The swimming
mo ili y o PcPCL1606 is enhanced by he dis up ion o HPR p oduc ion. Mu an s
de icien in HPR p oduc ion, e ealed ha colony mo phology, adhesion and ypical
ai –liquid in e phase pellicles we e all dependen on HPR p oduc ion. The ole o HPR
p oduc ion in bio ilm a chi ec u e was analyzed a he ini ial s age o bio ilm ma u a ion
using low- h ough low cells chambe s . These expe imen s e ealed ha he HPR
mu an cells had less igh unions han hose p oducing HPR, sugges ing an in ol emen
o HPR in he p oduc ion o he bio ilm ma ix by PcPCL1606.