Potential protective role of reactive astrocytes in the periventricular parenchyma in congenital hydrocephalus
Abstract
Background Cerebrospinal fluid accumulation in hydrocephalus produces an elevation of intraventricular pressure with pathological consequences on the periventricular brain parenchyma including ischemia, oedema, oxidative stress, and accumulation of metabolic waste products. Here we studied in the hyh mouse, an animal model of congenital hydrocephalus, the role of reactive astrocytes in this clinical degenerative condition. Materials and Methods Wild type and hydrocephalic hyh mice at 30 days of postnatal age were used. Three metabolites related to the oxidative and neurotoxic conditions were analysed in ex vivo samples (glutathione, glutamine and taurine) using High Resolution Magic Angle Spinning (HR-MAS). Glutathione synthetase and peroxidase, glutamine synthetase, kidney-type glutaminase (KGA), and taurine/taurine transporter were immunolocated in brain sections. Results Levels of the metabolites were remarkably higher in hydrocephalic conditions. Glutathione peroxidase and synthetase were both detected in the periventricular reactive astrocytes and neurons. Taurine was mostly found free in the periventricular parenchyma and in the reactive astrocytes, and the taurine transporter was mainly present in the neurons located in such regions. Glutamine synthetase was found in reactive astrocytes. Glutaminase was also detected in the reactive astrocytes and in periventricular neurons. These results suggest a possible protective response of reactive astrocytes against oxidative stress and neurotoxic conditions. Conclusions Astrocyte reaction seems to trigger an anti-oxidative and anti-neurotoxic response in order to ameliorate pathological damage in periventricular areas of the hydrocephalic mice.
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Programme of the Stirling Meeting Regulations for Poster Presenters Fees and Travel Arrangements Please note! Early registration with reduced rates ends on May 15th 2016. Programme SRHSB Stirling Scotland 2016 Wednesday 29th June to Saturday 2nd July 2016 University of Stirling Wednesday 29th June Executive Timings only 10.30 – 13.30 Executive Meeting (with Lunch) ……………………………………………………………………………………………………………………………………………… Conference opens for delegates REGISTRATION 12.30 – 19.00 (Local Organizing Committee/SBHScotland) 13.00 – 17.00 Pre Meetings Experimental Hydrocephalus (Hazel Jones) Clinical Cases (Roger Bayston; Marcus Pauly) 18:30 Coaches leave for Dan Young Building (The Spina Bifida Hydrocephalus Scotland Family Support Centre) 19.00 Welcome Reception by the Minister for Health Quality, Scottish Government and Informal Presentations by SBHScotland staff. 21.00 Coaches return to Stirling Court Hotel Thursday 30th June REGISTRATION 08.00 – 13.30 (Local Organizing Committee/SBHScotland)
8.45 Opening Remarks Andy Wynd and Rhoda Abel 9.00 – 11.00 Special Symposium: Genetics of Hydrocephalus and Spina Bifida Chair: Jo Glazier, Margo Whiteford Dr. Kate Storey - Mechanisms regulating neural differentiation Prof Andrew Copp - Genetic requirements for fusion of the neural folds as the neural tube closes 11.00 - 11.15 Coffee 11.15 - 12.30 Session 1 NTD: From embryogenesis to clinical aspects Chairs: Carole Sobkowiak, Andrew Copp 11.15 – 11.30 (Paper 1) Inositol for prevention of neural tube defects: the PONTI pilot randomised controlled trial Andrew J. Copp, Kit-Yi Leung, Victoria Gay, Katie Burren, Kevin Mills, Lyn S. Chitty, Nicholas D. E. Greene, London, UK 11.30 – 11.45 (Paper 2) Fusion of the neural folds during neural tube closure: defining the genetic basis Andrew J. Copp, Ana Rolo, Dawn Savery, Sarah Escuin, Sandra C. De Castro, Hannah E. J. Armer, Peter M. G. Munro, Matteo A. Mole, Nicholas D. E. Greene, London, UK 11.45 – 12.00 (Paper 3) Incidence of Tethered cord in children with Sacral Dimple Kashif Chauhan, Mayriam Guessoum, Gulwish Moghul , Nia Fraser, Alan Williams, Sharon Scriven, Manoj Shenoy, Nottingham, UK 12.00 – 12.15 (Paper 4) Health Care Problems of Refugees with Spina Bifida in Germany Reinhold Cremer, Cologne, Germany 12.20 Society Photograph 12.40 – 13.40 Lunch 14:00 Coaches Leave 15:00 Society Outing and Loch Lomond Cruise 17:15 Coaches leave to return to Stirling Court Hotel 19.00 Dinner at Stirling Court Hotel Friday 1st July REGISTRATION 08.00 – 13.30 (Local Organizing Committee/SBHScotland)
09.00 – 09.45 Special Lecture 1: Mark Hamilton – The Role for Endoscopic Third Ventriculostomy in the Adult Hydrocephalus Patient Chair: Pat McAllister 10.00 -10.45 Session 2 Neurosurgery Chair: Mark Hamilton, Maria Cartmill 10.00 – 10.15 (Paper 5) Endoscopic Third Ventriculostomy in the Under Ones is a Successful Strategy for Managing Hydrocephalus Maya Kommer, Emer Campbell, Roddy O'Kane, Glasgow, UK 10.15 – 10.30 (Paper 6) Flexible Neuroendoscopy: A Buyer’s Guide Aditaya Kumar, Emer Campbell, Roddy O'Kane, Glasgow, UK 10.30 – 10.45 (Paper 7) Title: Lumbar-Peritoneal Shunting in Hydrocephalus: Preventing Chiari Malformations and Decreasing Shunt Revisions Carolyn Harris, Neena I. Marupudi, Tanya Pavri, Rasanjeet Singh, Abilash Haridas, Steven D. Ham D.O., Sandeep Sood,, Detroit, Michigan, USA 10.45 – 11.00 Coffee 11.00 – 12.30 Session 3 Guthkelch Award Finalists Chairs: Carolyn Harris; Montserrat Guerra 11.00 - 11.15 (Paper 8) Mortality of patients with myelomeningocele - a population based study Joel Haakon Borgstedt-Bakke, Mikkel Mylius Rasmussen Aarhus,Denmark 11.15 – 11.30 (Paper 9) Corpus callosum structure and auditory interhemispheric transfer in spina bifida myelomeningocele Kailyn A. Bradley, Jenifer Juranek, Anna Romanowska-Pawliczek, H. Julia Hannay, Paul T. Cirino, Maureen Dennis, Larry A. Kramer, and Jack M. Fletcher Houston, TX, USA; New York, NY, USA; Toronto, ON, Canada 11.30 – 11.45 (Paper 10) Aquaporin-1 expression in human choroid plexus development and its variation in Arnold Chiari type I and lissencephaly pathologies Leandro Castañeyra-Ruiz Agustín Castañeyra-Perdomo, Ibrahim González-Marrero, Emilia M.Carmona-Calero, Gundela Meyer, Leandro Castañeyra-Ruiz La Laguna, Tenerife, Islas Canarias, Spain; Puerto del Rosario, Fuerteventura, Spain; La Laguna, Tenerife, Islas Canarias, Spain 11.45 – 12.00 (Paper 11) Potential protective role of reactive astrocytes in the periventricular parenchyma in congenital hydrocephalus
María García-Bonilla*, Kirill Shumilov, Ana Peñalver, Laura Castilla, Francisco J. Alonso, Javier Márquez, María-Luisa García-Martín, Carmen Muñoz, Antonia Gutierrez, Antonio J. Jimenez Malaga, Spain 12.00 – 12.15 (Paper 12) Neonatal hydrocephalus is a result of a block in folate handling and metabolism involving 10 formyl tetrahydrofolate dehydrogenase. Naila Naz, Alicia Requena Jimenez, Anna Sanjuan-Vilaplana, Megan Gurney, Jaleel Miyan Manchester, UK 12.15 – 12.30 (Paper 13) Cognitive rehabilitation of executive functions in adults with spina bifida: efficacy of goal management training Jan Stubberud, Donna Langenbahn, Brian Levine, Johan Stanghelle, Anne-Kristine Schanke Nesoddtangen, Norway; New York,NY, USA; Toronto, Canada; Oslo, Norway 12.30 – 13.30 Lunch 13.30 - 14.15 AGM 14.30 – 15.45 Session 4 Hydrocephalus: experimental and clinical aspects Chair: Hazel Jones; Jaleel Miyan 14.15 – 14.30 (Paper 14) Novel method for assessing pressure-induced neuronal injury patterns in vitro. Michael E. Smith[1], Ramin Eskandari[1,2] Charleston, South Carolina, USA 14.30 - 14.45 (Paper 15) Ventricular zone disruption in human neonates with posthemorrhagic hydrocephalus James P. (Pat) McAllister II , Maria Montserrat Guerra , Esteban M.Rodriguez , Diego M. Morales , and David D. Limbrick Jr, Saint Louis, Missouri, USA and Valdivia, Chile 14.45 – 15.00 (Paper 16) Subcommissural organ (SCO) encapsulated cells to promote neurogenesis in hydrocephalic HTx rats Maryoris Jara, Mario Flores, Karin Vío, Ignacio Moreno, Sara Rodríguez, Esteban M Rodríguez1, Montserrat Guerra, Valdivia, Chile 15.00 - 15.15 (Paper 17) Neural stem cells differentiate into multiciliated ependymal cells in vitro and after grafting into the cerebrospinal fluid of hydrocephalic HTx rats Roberto Henzi, Karin Vío, Montserrat Guerra, Pat McAllister, Conrad Johanson, Esteban M. Rodríguez, Saint Louis, Missouri, USA; Providence, Rhode Island, USA; Valdivia, Chile 15.15 - 15.30 (Paper 18) Brain viscoelasticity and headache in chronically shunted hydrocephalus using Magnetic Resonance Elastography
Kristy Tan, Avital Meiri, Adam L. Sandler, Rick Abbott, James T. Goodrich, Eric Barnhill, Mark E. Wagshul Bronx, NY, USA; Berlin, Germany; 15.30 – 15.45 Coffee (Delegates encouraged to final view Posters) 15.45 -16.30 Special Lecture 2: Roger Bayston – The Zika Virus and Microcephaly: Influence of External Infectious Agents on the Fetus Chair: Helen Williams 16.30 – 17.15 Session 5 Oral Poster Presentations 3-5 minutes each (6 posters) Moderator: Andy Wynd; Waheed Ashraf 16.30 - 16.37 (Poster 1) Changes in clinical practice regarding brain imaging for patients with myelomeningocele and hydrocephalus due to increased awareness of risks from medical radiation Gregory Smookler, Alexis Daevenport, Holly Lai, Los Angeles, USA 16.37 - 16.44 (Poster 2) Spinal dysraphism – experience of a southern Italy department and analysis of 141 cases Carmen D'Aleo, Caltanissetta, Italy 16.44 - 16.51 (Poster 3) Enhanced attentional performance following pediatric goal management training in children with spina bifida: a pilot study Jan Stubberud Anne-Kristine Schanke, Lisbeth C. Brøndberg, Marie Hoff, Karen Grimsrud, Wenche F. Wilhelmsen, Pål-Erik Plaum, Trond Haagensen, Tove Helland, Julie B. Berge, Astri Å. Andersen, Marianne Løvstad, Oslo, Norway 16.51 - 16.58 (Poster 4) A pilot study of pediatric goal management training in children with spina bifida: informant and self reports six months post-training Jan Stubberud, Anne-Kristine Schanke, Lisbeth C. Brøndberg, Marie Hoff, Karen Grimsrud, Wenche F. Wilhelmsen, Pål-Erik Plaum, Trond Haagensen, Tove Helland, Julie B. Berge, Astri Å. Andersen, Marianne Løvstad, Oslo, Norway 16.58 - 17.05 (Poster 5) Closing the complex: a neurosurgical unit’s experience with open myelomeningoceles Aditaya Kumar, Roddy O'Kane, Emer Campbell, Glasgow, UK 17.05 - 17.15 Coffee
17.15 - 17.45 Session 6 History Chair: Reinhold Cremer 17.15 - 17.30 60th SRHSB Archivist Presentation - Ewen A. McKinnon 17.30 – 17.45 Scotland’s Contribution to Spina Bifida over the years – Roddy O’Kane 19.00 Coaches leave for Annual Dinner and presentation of Guthkelch Award 19.30 Annual Dinner and with Guthkelch Award Winner Presentation at Stirling Castle 23:45 Coaches return to Stirling Court Hotel Saturday 2nd July REGISTRATION 09.00 – 13.30 (LOC/SBHScotland) 09.30 – 10.45 Session 7 Hydrocephalus Chairs: Ian Pople, Laurence Watkins 09.30 - 09.45 (Paper 19) Ventriculosubgaleal shunts can be used to delay permanent csf drainage in premature and low birth-weight babies Maya Kommer, Roddy O'Kane, Emer Campbell, Glasgow, UK 09.45 - 10.00 (Paper 20) An audit of ventriculitis in external ventricular drainage after introduction of an antibacterial catheter over a seven year period. Liam Cain, Roger Bayston, Shazia Javed, Nottingham, UK 10.00 - 10.15 (Paper 21) Reducing ventricular shunt malfunction in the adult patient Mark G. Hamilton, Geberth Urbaneja, Aaron Hockley, Albert Isaacs, Calgary, Canada 10.15 - 10.30 (Paper 22) Investigating glial mechanisms of shunt obstruction in hydrocephalus Carolyn Harris, William Shain, Brian Hanak, M.D., Steven Ham, D.O. Detroit, Michigan, USA; Seattle, Washington, USA 10.30 - 10.45 (Paper 23) Is cerebral ventricle filling a low pressure phenomenon? Helen Williams, London, UK. 10.4511.00 (Paper 24) Surgery for intracranial arachnoid cysts in children—a prospective longterm study Katrin Rabiei, Mats Johansson Högfeldt, Roberto Doria-Medina, MagnusTisell Gothenburg, Sweden 11.00 – 11.15 Coffee 11.15 – 12.30 Session 8 Urology and Psychology Chair: Chris Driver; David Nash
11.15 - 11.30 (Paper 25) Experience of intradetrusor injection of Botulinum toxin A in children: a 10-year retrospective review Peeraully R, Lam C, Mediratta N, Williams A, Shenoy M, Fraser N, Nottingham, UK 11.30 - 11.45 (Paper 26) Outcomes of urinary incontinence surgeries in spina bifida patients. Vinod Raj, Varun Hatiramani, Swathi Krishna, Santosh Karmarkar, Mumbai, India 11.45 - 12.00 (Paper 27) Stronger links, stronger families; supporting families affected by spina bifida and hydrocephalus to be all they can be Sophie Teear, Rachel Lawson, Kim McKay, Vikki Rothero, Cumbernauld, UK 12.00 - 12.15 (Paper 28) Developing a screening methodology for improving quality of life in children and young people with hydrocephalus. Chloe Geagan, Kirsten Verity, Edinburgh, UK 12.15 – 12.45 Break – (Packed Lunch collection) (judges discussion) 12.45 -13.00 Welcome to St. Louis – Pat McAllister 13.00 – 13.15 Announcement of prize winners (to be announced by Helen Williams, Marcus Pauly and Reinhold Cremer and Pat McAllister) Best Clinical Presentation Best Scientific Presentation Poster Prize President’s Prize 13.15 – 13.30 Hosts and President Closing Remarks 13.30 Conference Close …………………………………………………………………………………………………………………………………….. Executive Timings only 14.00 - 16.00 Executive Meeting Regulations for poster presenters: Posters will be presented in a special poster session on Friday afternoon, not in a poster walk. Posters may be presented for 1-2 minutes by a designated reviewer, followed by a statement and answers to questions from the author (max. 5 minutes per poster). Posters will also be presented on panels for viewing during the breaks. Authors are asked be present at their posters for discussion. Posters should be in place during Thursday morning and removed Saturday at close of conference. Fastening strips will be provided. Preferred poster measures: height 1180 mm, width 841 mm.
Conference registration and housing details: STIRLING 2016 - SRHSB Conference Registration and Accommodation Package The SRHSB has a proud history of combining a high quality international clinical and scientific research conference with a truly “research family” focus for all those who work in and around hydrocephalus and spina bifida. Not only will you be able to hear the latest research news across scientific and clinical areas but you will be part of a growing body of specialists in the field. Couple this with an ideal opportunity to network worldwide and this year hear about the history and achievements of the SRHSB as it celebrates its 60th anniversary and to visit Scotland the home of Spina Bifida Hydrocephalus Scotland which also celebrates its 50th anniversary. www.sbhscotland.org.uk Early Bird Rates All packages are fully inclusive (no hidden additional charges or taxes) and have early Bird rates automatically discounted on the price you pay dependent on date when you pay online. By May 15, 2016 After May 15, 2016 After June 15, 2016 Registration Member £450 £500 £550 on site Non-Member £525 £550 £600 on site Student £325 £350 £400 on site Student Day Rate £120 £120 £140 on site Accompanying Person £310 £360 £400 on site Accommodation per night Stirling Court Hotel (meeting site) By May 15, 2016 After May 15, 2016 Single £79 £79 based on availability Willow Court (Campus) Housing (5 minute walk to meeting hotel) Single £44 £44 based on availability Colessio Hotel (5 star, 12 minute taxi to meeting hotel) Club Room £109 £109 based on availability Premium Room £149 £149 based on availability The full Conference and accommodation package includes on site accommodation at the Stirling Court Hotel (http://www.stirlingcourthotel.com/) which is also the venue for the main conference sessions. In addition the cost includes an Opening Reception, Society Outing to Loch Lomond and Gala Dinner in a stunning venue for all resident delegates.
Detailed meeting information and program access: All attendees and members are encouraged to use the Whova app! This is a new feature that will be running throughout the conference and can be used immediately to find travel details, for example. It is now live and everyone who has a smart phone (either Android or IOS) should be able to download the app, register and gain access to the latest programme, (http://www.whova.com/portal/sicss_201606) and also access to all travel arrangements (under the logistics icon). For those who have a blackberry or want to access a web version then the URL is https://whova.com/webapp/e/sicss_201606. The travel information is presented as hyperlinks to the visit Scotland site and others so if you want to copy these to the SRHSB website as well then that will be fine.