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Morphological traits of surveillant/activated microglia during an experimentally induced acute neuroinflammatory process

Fernández-Arjona, María del Mar,López-Ávalos, María Dolores,Mateos-Grondona, Jesús,Fernández-Llebrez, Pedro

Published
2016-07-01
Repository
riuma.uma.es
Language
en

Abstract

Several studies show that morphological changes of microglia over the course of inflammation are tightly coupled to function. However the progressive transformation into activated microglia is poorly characterized. AIMS: This study aimed to establish a spatiotemporal correlation between quantifiable morphological parameters of microglia and the spread of an acute ventricular inflammatory process. METHODS: Inflammation was induced by a single injection of the enzyme neuraminidase within the lateral ventricle of rats. Animals were sacrificed 2, 4 and 12 hours after injection. Coronal slices were immunostained with Iba1 to label microglia and with IL1β to delimit the spread of inflammation. Digital images were obtained by scanning the labelled sections. Single microglia images were randomly selected from periventricular areas of caudate putamen, hippocampus and hypothalamus. FracLac for ImageJ software was used to measure the following morphological parameters: fractal dimension, lacunarity, area, perimeter and density. RESULTS: Significant differences were found in fractal dimension, lacunarity, perimeter and density of microglia cells of neuraminidase injected rats compared to sham animals. However no differences were found in the parameter “area”. In hipoccampus there was a delay in the significant change of the measured parameters. These morphological changes correlated with IL1β-expression in the same areas. CONCLUSIONS: Ventricular inflammation induced by neuraminidase provokes quantifiable morphological changes in microglia restricted to areas labelled with IL1β. Morphological parameters of microglia such as fractal dimension, lacunarity, perimeter and density are sensitive and valuable tools to quantify activation. However, the extensively used parameter “area” did not change upon microglia activation.

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12/5/2016 RoundCube::AbstractSubmissionFENS2016 https://gw.uma.es/roundcube/?_task=mail&_action=print&_uid=2412&_mbox=INBOX&_safe=1&_extwin=1 1/3 Subject AbstractSubmissionFENS2016 From FENS2016Secretariat<[email protected]> To <[email protected]> Date 02.02.201613:14 Priority Highest DearMsMarFernandezArjona, ThankyouverymuchforusingtheFENSForum2016AbstractSubmissionSystem. YourAbstracthasbeensaved.ThenumberofyourabstractisFENS2281. Pleasenotethatyourabstractissavedbutnotsubmitted.Youmaymakechangesupuntiltheabstractsubmissiondeadlineof2 February2016,23:59hoursCET. After2February2016,23:59hoursCET,theabstractswillautomaticallybesubmittedtotheForumandnofurtherchanges willbepossible. Ifyouhavequestionsorremarkspleasecontact:[email protected] Inordertoedityoursubmittedabstract,pleasefollowthislink IfyouwishtocontinuetotheFENSIBRO/PERCtravelgrantapplication,pleasefollowthislink 12/5/2016 RoundCube::AbstractSubmissionFENS2016 https://gw.uma.es/roundcube/?_task=mail&_action=print&_uid=2412&_mbox=INBOX&_safe=1&_extwin=1 2/3 The"Abstractsubmission"andthe"TravelGrant"buttonscanbefoundatthebottomofthepageafterloggingintoyourregistration overviewpage. KindRegards, FENSForum2016Secretariat ThisemailisautomaticallygeneratedonbehalfofFENSForum2016,pleasedonotreplytothisemail.Forfurtherinformation regardingthecontentofthisemailpleasecontact[email protected] Incaseyouwishtodeleteorwithdrawyourabstract,pleasesendtherequest(mentioningtheabstractnumber)tothe AbstractTeamat:[email protected] Pleasefindanoverviewofyoursavedabstractbelow: Theme C.Disordersofthenervoussystem Topic C13.Neurotoxicity,inflammation,andneuroprotection Presentation preference Posterpresentation Abstracttitle Morphologicaltraitsofsurveillant/activatedmicrogliaduringanexperimentallyinducedacute neuroinflammatoryprocess Severalstudiesshowthatmorphologicalchangesofmicrogliaoverthecourseofinflammationaretightly coupledtofunction.Howevertheprogressivetransformationintoactivatedmicrogliaispoorlycharacterized. AIMS:Thisstudyaimedtoestablishaspatiotemporalcorrelationbetweenquantifiablemorphological parametersofmicrogliaandthespreadofanacuteventricularinflammatoryprocess. METHODS:Inflammationwasinducedbyasingleinjectionoftheenzymeneuraminidasewithinthelateral ventricleofrats.Animalsweresacrificed2,4and12hoursafterinjection.Coronalsliceswereimmunostained withIba1tolabelmicrogliaandwithIL1βtodelimitthespreadofinflammation.Digitalimageswereobtainedby scanningthelabelledsections.Singlemicrogliaimageswererandomlyselectedfromperiventricularareasof caudateputamen,hippocampusandhypothalamus.FracLacforImageJsoftwarewasusedtomeasurethe followingmorphologicalparameters:fractaldimension,lacunarity,area,perimeteranddensity. RESULTS:Significantdifferenceswerefoundinfractaldimension,lacunarity,perimeteranddensityofmicroglia cellsofneuraminidaseinjectedratscomparedtoshamanimals.Howevernodifferenceswerefoundinthe parameter"area".Inhipoccampustherewasadelayinthesignificantchangeofthemeasuredparameters. ThesemorphologicalchangescorrelatedwithIL1βexpressioninthesameareas. 12/5/2016 RoundCube::AbstractSubmissionFENS2016 https://gw.uma.es/roundcube/?_task=mail&_action=print&_uid=2412&_mbox=INBOX&_safe=1&_extwin=1 3/3 CONCLUSIONS:Ventricularinflammationinducedbyneuraminidaseprovokesquantifiablemorphological changesinmicrogliarestrictedtoareaslabelledwithIL1β.Morphologicalparametersofmicrogliasuchas fractaldimension,lacunarity,perimeteranddensityaresensitiveandvaluabletoolstoquantifyactivation. However,theextensivelyusedparameter"area"didnotchangeuponmicrogliaactivation.   AllrightsReservedtoKenesInternational