Synthesis of fluorescent dendrimeric antigen efficiently internalized by human dendritic cells
Abstract
A new fluorescent dendrimeric antigen (DeAn) based on a dendron with amoxicilloyl terminal groups has been synthetized. The synthesis implies a novel class of all-aliphatic polyamide dendrimer (BisAminoalkylPolyAmide Dendrimers, or BAPAD).[1] The introduction of a cystamine core allows the incorporation of this dendrons into a 1,8-naphthalimide fluorofore functionalized with a maleimide group. The fluorescence properties of this DeAn has been studied and compared with the properties of an equivalent dendron possessing amino-terminal groups. This DeAn has been used as a synthetic antigen in a biomedical assay that tests the amoxicillin sensitivity of dendritic cells (DC) from tolerant and allergic patients.
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SYNTHESIS OF FLUORESCENT DENDRIMERIC ANTIGEN EFFICIENTLY INTERNALIZED BY HUMAN DENDRITIC CELLS Pablo Mesa-Antúnez,1,2 Daniel Collado,1,2 Yolanda Vida,1,2 Francisco Najera,1,2 Tahia Fernandez.3 Maria Jose Torres,3,4 and Ezequiel Perez-Inestrosa.1,2 1University of Malaga, IBIMA, Depart. of Organic Chemistry, 29071 Malaga, Spain 2Andalusian Centre for Nanomedicine and Biotechnology, BIONAND, Malaga, Spain 3Research Laboratory, Regional University Hospital of Malaga-IBIMA, Malaga, Spain 4Allergy Service, Regional University Hospital of Malaga-IBIMA, Malaga, Spain [email protected] A new fluorescent dendrimeric antigen (DeAn) based on a dendron with amoxicilloyl terminal groups has been synthetized. The synthesis implies a novel class of allaliphatic polyamide dendrimer (BisAminoalkylPolyAmide Dendrimers, or BAPAD).[1] The introduction of a cystamine core allows the incorporation of this dendrons into a 1,8-naphthalimide fluorofore functionalized with a maleimide group. The fluorescence properties of this DeAn has been studied and compared with the properties of an equivalent dendron possessing amino-terminal groups. This DeAn has been used as a synthetic antigen in a biomedical assay that tests the amoxicillin sensitivity of dendritic cells (DC) from tolerant and allergic patients. [1] Ruiz-Sanchez, A.J.; Mesa-Antunez, P.; Barbero, N.; Collado, D.; Vida, Y.; Najera, F.; Perez-Inestrosa, E.; Polymer Chemistry 2015, 6, 3031.