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Lower prokaryotic leucine incorporation rates under in situ pressure than under decompressed conditions in the deep north Atlantic

Amano-Sato, C.,Sintes, Eva,Reinthaler, T.,Varela, Marta María,Utsumi, M.,Herndl, G.J.

Abstract

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LOWER PROKARYOTIC LEUCINE INCORPORATION RATES UNDER IN SITU PRESSURE THAN UNDER DECOMPRESSED CONDITIONS IN THE DEEP NORTH ATLANTIC P oka yo ic ac i i y and communi y composi ion is highly dep h-s a i ied in he oceanic wa e column e lec ing he inc easing ecalci ance o dissol ed o ganic ma e and dec easing empe a u e wi h dep h. The ole o inc easing hyd os a ic p essu e in con olling deep ocean mic obial ac i i y is less well-s udied. To de e mine he in luence in hyd os a ic p essu e on he e o ophic mic obial ac i i y, an in si u incuba o was deployed in he No h A lan ic Ocean a a dep h be ween 500 o 2000 m. The in si u incuba o was p og ammed o collec and incuba e p oka yo es unde he wa e a e adding 3H-leucine and o ix a ce ain olume o he incuba ed samples a speci ic ime in e als (3 o 10 h depending on he dep h). P oka yo ic leucine inco po a ion ob ained unde in si u p essu e condi ions was gene ally lowe han ha on decomp essed samples incuba ed on boa d. Ra ios o in si u p oka yo ic leucine inco po a ion o decomp essed condi ions dec eased wi h inc easing dep h. Ou esul s sugges ha bulk he e o ophic p oka yo ic p oduc ion in he deep sea migh be lowe han expec ed.