Draft Genome Sequence of the Tyramine Producer Enterococcus durans Strain IPLA 655

D a Genome Sequence o he Ty amine P oduce En e ococcus du ans S ain IPLA 655 Vic o Lade o, Daniel M. Lina es, Bea iz del Rio, Ma ia Fe nandez, M. C uz Ma in, Miguel A. Al a ez Ins i u o de P oduc os Lác eos de As u ias, IPLA-CSIC, Paseo Rio Lina es s/n, Villa iciosa, As u ias, Spain We he e epo a 3.059-Mbp d a assembly o he genome o En e ococcus du ans s ain IPLA 655. This dai y isola e p o ides a model o s udying he egula ion o he biosyn hesis o y amine (a oxic compound). These esul s should aid ou unde s and- ing o y amine p oduc ion and allow y amine accumula ion in ood o be educed. Recei ed 3 Ap il 2013 Accep ed 4 Ap il 2013 Published 16 May 2013 Ci a ion Lade o V, Lina es DM, del Rio B, Fe nandez M, Ma in MC, Al a ez MA. 2013. D a genome sequence o he y amine p oduce En e ococcus du ans s ain IPLA 655. Genome Announc. 1(3):e00265-13. doi:10.1128/genomeA.00265-13. Copy igh © 2013 Lade o e al. This is an open-access a icle dis ibu ed unde he e ms o he C ea i e Commons A ibu ion 3.0 Unpo ed license. Add ess co espondence o Miguel A. Al a ez, [email p o ec ed]. En e ococcus du ans is a mino componen o he in es inal mi- c obio a o humans and domes ic animals (1). I is commonly ound in animal-de i ed ood p oduc s, especially milk and cheese (2). This bac e ium p oduces y amine—a biogenic amine (BA) equen ly p esen in e men ed oods—by deca boxyla ing y osine. The inges ion o ood wi h high y amine concen a ions causes an in oxica ion, known as he cheese eac ion, cha ac e - ized by mig aine, hype ension, e c. (3). Ty amine p oduc ion in bac e ia has been ela ed o ene gy p oduc ion and he acidic s ess esponse (4). E.du ans s ain IPLA 655, which was isola ed om a adi- ional cheese (5), p o ides a model o esea ch in o y amine p oduc ion (6–9). A genomic lib a y o 0.5 kbp was cons uc ed and subjec ed o pai ed-end sequencing (p o iding app oxi- ma ely 150- old co e age) using a HiSeq 1000 Sys em sequence (Illumina) a he Beijing Genomics Ins i u e (China). Quali y- il e ed eads we e assembled in o con igs using Vel e so wa e (h p://www.ebi.ac.uk/~ze bino/ el e /). Anno a ion was pe - o med using he P oka yo ic Genomes Anno a ion Pipeline (PGAAP) a he NCBI (h p://www.ncbi.nlm.nih.go /genomes /s a ic/Pipeline.h ml), and imp o ed wi h esul s ob ained om BLAST analysis (h p://blas .ncbi.nlm.nih.go ). The d a genome sequence o E. du ans IPLA 655 in ol es 3,059,052 bp, a GC con en o 37.7%, and 141 con igs anging om 201 o 203,388 bp. I encodes 2,853 p edic ed coding sequences. Single p edic ed copies o 16S, 23S, and 5S RNA genes we e ound, as well as 51 genes o RNAs. In ag eemen wi h i s dai y o igin, E. du ans IPLA 655 may me abolize lac ose; a lac ose phospho ans e ase ope on (lacXGEFDCBAR) was iden i ied in i s genome. Howe e , no ex a- cellula caseinoly ic p o ease genes we e ound. The genome con ains he y amine p oduc ion clus e , bu no o he BA clus e s. BA p oduc ion clus e s gene ally include a gene simila o hose encoding he co esponding aminoacyl- RNA syn he ase, bu wi h unp o en unc ion. Su p isingly, analysis o he E. du ans IPLA 655 genome showed no o he y osyl- RNA syn he ase gene besides he y osyl- RNA syn he ase-like gene ( y S) in he y- amine clus e . This con i ms ha y S encodes a y osyl- RNA syn he ase. Up egula ed a a low pH (9), when y osine is deca - boxyla ed o coun e ac acidic s ess, he inc eased p oduc would ensu e he supply o y osine o p o ein biosyn hesis. No e idence o i ulence- ela ed genes o an ibio ic esis ance genes was seen. A leas h ee p ophages we e de ec ed; hese we e mos homologouswi hp ophageso E. aecium.Somegenesin ol ed in plasmid mobiliza ion o eplica ion we e de ec ed, along wi h wo pu a i e oxin/an i- oxin plasmid s abiliza ion sys ems, in ag eemen wi h he p esence o a leas h ee plasmids (da a no shown). The genome sequence o E. du ans IPLA 655 should aid ou unde s anding o he gene ic egula ion and physiological signi - icance o y amine biosyn hesis, and pe haps sugges ways o e- ducing y amine accumula ion in ood. Nucleo ide sequence accession numbe s. The esul s o his whole-genome sho gun p ojec we e deposi ed in he DDBJ/ EMBL/GenBankda abaseunde accession numbe AOSM00000000. The e sion o he genome desc ibed he e has he accession num- be AOSM01000000. ACKNOWLEDGMENTS This wo k was unded by he Spanish Minis y o Economy and Compe - i i eness (AGL2010-18430) and CSIC (201270E144). B.D.R. and D.M.L. a e bene icia ies o JAE-DOC con ac s (CSIC). 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