Dual 5-HT3and 5-HT6Receptor Antagonist FPPQ Normalizes Phencyclidine-Induced Disruption of Brain Oscillatory Activity in Rats

Received: November 8, 2021; Revised: December 22, 2021; Accepted: January 11, 2022 425 Copyedited by: © The Author(s) 2022. Published by Oxford University Press on behalf of CINP. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact [email protected] Rapid Communication Dual 5-HT3 and 5-HT6 Receptor Antagonist FPPQ Normalizes Phencyclidine-Induced Disruption of Brain Oscillatory Activity inRats AnnaCastañé, MontserratCano, LuisRuiz-Avila, LluísMiquel-Rio, PauCelada, FrancescArtigas, Maurizio S.Riga Departament de Neurociències i Terapèutica Experimental, CSIC-Institut d’Investigacions Biomèdiques de Barcelona, Barcelona, Spain (Drs Castañé, Riga, MrMiquel-Rio, Celada, and Artigas); Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain (Drs Castañé, Riga, MrMiquel-Rio, Celada, and Artigas); Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Instituto de Salud Carlos III, Madrid, Spain (Drs Castañé, Riga, MrMiquel-Rio, Celada, and Artigas); Spherium Biomed, Barcelona,Spain(Drs Cano and Ruiz-Avila). Present address: Facultat de Medicina, Universitat de Vic-Universitat Central de Catalunya (UVic-UCC), Vic, Spain (Dr Castañé). Present address: Andalusian Center for Molecular Biology and Regenerative Medicine-CABIMER, CSIC, Seville 41092, Spain (Dr Riga). Correspondence: Anna Castañé, PhD, Facultat de Medicina, Carretera de Roda, 70, 08500 Vic, Spain ([email protected]). Authors A.C.and M.S.R.contributed equally to this article. Abstract Schizophrenia is a severe mental disorder featuring psychotic, depressive, and cognitive alterations. Current antipsychotic drugs preferentially target dopamine D2-R and/or serotonergic 5-HT2A/1A-R. They partly alleviate psychotic symptoms but fail to treat negative symptoms and cognitive deficits. Here we report on the putative antipsychotic activity of (1-[(3-fluorophenyl) sulfonyl]-4-(piperazin-1-yl)-1H-pyrrolo[3,2-c]quinoline dihydrochloride) (FPPQ), a dual serotonin 5-HT3-R/5-HT6-R antagonist endowed with pro-cognitive properties. FPPQ fully reversed phencyclidine-induced decrease of low-frequency oscillations in the medial prefrontal cortex of anaesthetized rats, a fingerprint of antipsychotic activity. This effect was mimicked by the combined administration of the 5-HT3-R and 5-HT6-R antagonists ondansetron and SB-399 885, respectively, but not by either drug alone. In freely moving rats, FPPQ countered phencyclidine-induced hyperlocomotion and augmentation of gamma and high-frequency oscillations in medial prefrontal cortex, dorsal hippocampus, and nucleus accumbens. Overall, this supports that simultaneous blockade of 5-HT3R and 5-HT6-R-like that induced by FPPQ-can be a new target in antipsychotic drug development. Keywords: Schizophrenia, NMDA-R antagonists, 5-HT3-R, 5-HT6-R, antipsychotics applyparastyle "fig//caption/p[1]" parastyle "FigCapt" applyparastyle "fig" parastyle "Figure" International Journal of Neuropsychopharmacology (2022) 25(5): 425–431 https://doi.org/10.1093/ijnp/pyac003 Advance Access Publication: January 12, 2022 Rapid Communication Downloaded from https://academic.oup.com/ijnp/article/25/5/425/6504018 by Secretaria General Adjunta de Informatica user on 30 November 2022 426 | International Journal of Neuropsychopharmacology, 2022 Introduction Schizophrenia is a chronic and highly disruptive psychiatric disorder characterized by the presence of positive and negative symptoms and an important cognitive decline. Available drugs are partly effective for positive symptoms but fail in treating negative/cognitive symptomatology (Leucht et al., 2013). Firstand second-generation antipsychotic drugs preferentially antagonize dopamine D2-R and serotonin 5-HT2A-R, respectively. A third generation of drugs showing partial agonism at D2-R and 5-HT1A-R together with 5-HT2A-R antagonism has also been developed (Newman-Tancredi and Kleven, 2011). Despite new improvements, difficulties for an adequate treatment of schizophrenia persist. Novel strategies targeting the glutamatergic system, including mGluR2/3-R agonists, failed despite initial expectations (Adams etal., 2014; Bugarski-Kirola etal., 2017). Recently, Zajdel et al. (2021) proposed a novel mechanism to achieve antipsychotic and pro-cognitive properties by a nondopaminergic action. Based on evidence that 5-HT3-R and 5-HT6-R antagonists improve cognition when administered as adjuvant therapy to antipsychotics, they developed and tested a dualacting 5-HT3-R/5-HT6-R antagonist, FPPQ (1-[(3-fluorophenyl) sulfonyl]-4-(piperazin-1-yl)-1H-pyrrolo[3,2-c]quinoline dihydrochloride). The simultaneous blockade of 5-HT3-R/5-HT6-R by FFPQ dose-dependently prevented phencyclidine (PCP)- induced short-term memory deficits in the novel object recognition test (NORT) and reversed PCP-induced hyperlocomotion. PCP showed to activate thalamo-cortical circuits through a preferential blockade of NMDA-R in the reticular nucleus of the thalamus (Santana et al., 2011; Troyano-Rodriguez et al., 2014). This nucleus is composed exclusively of GABA neurons that tonically inhibit the rest of thalamic nuclei. In parallel with enhancing the activity of principal neurons in thalamo-cortical areas, PCP markedly reduced the power of low-frequency oscillations (LFO; 0.15–4 Hz) in the medial prefrontal cortex (mPFC) of anesthetized rats. All antipsychotic drugs tested so far, irrespectively of their pharmacological profile, reverse the decrease in LFO induced by PCP (Kargieman et al., 2007; Lladó-Pelfort etal., 2016; Van den Munkhof etal., 2017). Therefore, we extended the study by Zajdel etal. (2021) to test the ability of FPPQ to prevent or reverse PCP-induced effects on (1) LFO in the mPFC of anesthetized rats, and (2) hyperlocomotion and high-frequency oscillation (HFO) bands in awake freely moving rats. METHODS Animals Male albino Wistar rats (Charles River, France) weighing 250– 300g were used. Animals were kept in a controlled environment (12-hour-light/-dark cycle and 21°C ± 1°C) with food and water ad libitum. Experiments were in accordance with the NIH Guide for the Care and Use of Laboratory Animals, Animal (Scientific Procedure) Act 1986 and in accordance with the ARRIVE guideline (Kilkenny etal., 2010). Animal care followed European Union regulations (directive 2010/63 of September 22, 2010)and was approved by the Institutional Animal Care and Use Committees. Drugs PCP hydrochloride and ondansetron (OND) hydrochloride dihydrate (Sigma/RBI, Natick, MA, USA) were dissolved in saline and stored at −20°C until used. FPPQ (Zajdel etal., 2021) solutions were prepared daily in saline (i.v.), and in hydroxyl-propylßcyclodextrin 10% + glucose monohydrate 4.4% adjusted to pH 6–6.5 with NaOH 1M (i.p.). FPPQ was discovered by the scientific consortium of the Faculty of Pharmacy Jagiellonian University Medical College, University of Montpellier, and Institute of Pharmacology Polish Academy of Sciences (Zajdel etal., 2015) and licensed to Spherium Biomed (Barcelona, Spain). SB-399 885 (Sigma/RBI) solutions were prepared freshly in 1% Tween 80 (i.v.). Buprenorfine (Buprex®) and enrofloxacine (Enrovet®) were dissolved in saline. Doses are expressed as free base. All compounds were administrated in a final volume of 0.25–2mL/kg. In Vivo Electrophysiology in AnaesthetizedRats PCP (0.25–0.5mg/kg), OND (1.28mg/kg), SB-399 885 (10mg/kg), and FPPQ (3mg/kg) were administered i.v. Rats were anesthetized with chloral hydrate (induction: 400mg/kg i.p; maintenance: 50–70 mg/kg/h). Local field potentials were recorded in mPFC (coordinates AP + 3.4, L-0.7, DV-2.8 from bregma and skull; Paxinos and Watson, 2005) as previously described (Kargieman etal., 2007). After recording stable baseline activity (5 minutes), 1 or 2 doses of PCP were administered. After 2 minutes of stable PCP effect, FPPQ, OND, SB-399 885, or the combination of the latter 2 drugs were slowly administrated. Recordings were kept for 10 minutes after drug administration. Simultaneous Recordings and Locomotor Activity Measures in Freely MovingRats Rats were implanted with Plastics One electrodes (Roanoke, VA, USA) under isoflurane anesthesia (induction: 5%; maintenance: 1.5%–2%) as previously described (Riga et al., 2017). Animals were pretreated (30 minutes before anesthesia) with an analgesic (buprenorfine 0.05mg/kg, s.c.). Recording electrodes were implanted at mPFC (AP + 3.2, L-0.5, DV-2.1), nucleus accumbens (NAc; AP + 1.7, L-1.0, DV-6.8), and dorsal hippocampus (dHPC; AP-3.7, L-2.0, DV-2.1) (coordinates from bregma and skull; Paxinos and Watson, 2005). Aground screw (AP-8.7 L + .4) and 3 stabilizer screws were also implanted. Buprenorfine (0.05mg/kg s.c.) and a prophylactic antibiotic (enrofloxacine 7.5mg/kg s.c.) were given during 3days after surgery. Local field potential recordings were performed in a 40- × 40-cm open field using a digital Lynx system and Cheetah software (Neuralynx, Westlake Village, CA, USA). The signal was obtained at 3.2kHz sampling rate and filtered between 0.1 and 200 Hz. All recordings were subsequently down sampled 10 times before analysis. Simultaneously, locomotor activity was recorded during electrophysiological recordings and analyzed offline by the SMART video-tracking software (Harvard-Panlab, Barcelona, Spain). Recordings were performed 1 week after surgery. On the recording day, after 20 minutes of baseline recording, FPPQ (or vehicle [VEH]) and PCP were injected 45 minutes apart, and recordings were performed for 45 minutes after last injection. Finally, rats were killed by an anesthetic overdose, and the histological localization of electrodes was performed. Data and Statistical Analyses In anaesthetized rats, the power of LFO (0.15–4 Hz) was calculated for the last 2-minute period before PCP or drug administration and in 3 consecutive 2-minute periods before the end Downloaded from https://academic.oup.com/ijnp/article/25/5/425/6504018 by Secretaria General Adjunta de Informatica user on 30 November 2022 Copyedited by: Castañé et al. | 427 of recording (see below). Recordings were analyzed off-line with Spike2 software using built-in and self-developed scripts. Mean LFO power was quantified by averaging 6 consecutive 10-second periods (for each minute) and then generating the mean of 2 minutes. Fast Fourier Transformation with a resolution of 0.15 Hz was used. LFO power is expressed as μV2. For recordings in freely moving rats, raw data were imported to MatLab environment (MathWorks, MA, USA) for off-line power wavelet analysis using built-in and self-developed routines. The frequency bands analyzed were delta (0.2–4 Hz), theta (4–10 Hz), beta (10–30 Hz), gamma (30–80 Hz), and HFO (140–180 Hz). Data were averaged in 5-minute periods. Injection periods (5 minutes post-drug administrations) were excluded from analysis. Data were expressed as percentage of basal values in each time period. Oneor 2-way ANOVA (with treatment and time factors) were performed, followed by Duncan’s tests for post-hoc analysis, as appropriate. Locomotor activity data were analyzed by MannWhitney test. Data are shown as mean ± SEM. Statistical analysis was conducted by using GraphPad Prism version 6.Statistical significance has been set at the 95% confidence level (two-tailed). RESULTS Concurrent 5-HT3 and 5-HT6 Receptor Blockade Reverses PCP-Induced Decrease of LFO in mPFC PCP administration induced a dramatic decrease of LFO in the mPFC of anesthetized rats. Given the capacity of FPPQ to attenuate PCP-induced hyperactivity and recognition memory deficits (Zajdel etal., 2021), we examined its ability as well as that of OND (5-HT3-R antagonist) and SB-399 885 (5-HT6-R antagonist) to reverse PCP effects onLFO. FPPQ fully reversed the PCP-induced decrease in mPFC LFO (Fig. 1A,E,J), similarly to atypical antipsychotics (Fig. 1K, modified from Lladó-Pelfort etal., 2016). In contrast, OND (Fig. 1B,F,I) and SB-399 885 (Fig. 1C,G,I) failed to reverse the PCP-induced disruption of LFO in mPFC. Interestingly, the combination of both drugs (OND + SB-399 885) fully countered PCP effects on LFO (Fig. 1D,H,J), mimicking the effect of FPPQ. Figure 1A–D shows representative examples of the ability of these drugs to reverse the PCP-induced loss of LFO in mPFC (see statistical analyses in legend to Fig. 1). FPPQ Pretreatment Prevents PCP-Induced Alterations in Brain Oscillations and Hyperlocomotion in Freely MovingRats Acute FPPQ treatment did not evoke major changes on brain oscillatory activity in the mPFC, NAc, and dHPC of freely moving rats. No differences between VEHand FPPQ-pretreated values (from 25 to 65 minutes) were found in any case (Fig. 2A–C). PCP administration increased the power of beta, gamma, and HFO bands in the mPFC of VEH-treated rats (n = 5) (Fig. 2A). Moreover, PCP enhanced theta, beta, and HFO oscillations in NAc (Fig. 2B) and gamma and HFO in dHPC (Fig. 2C) (n = 6 for each area). PCPinduced changes in mPFC theta and NAc delta bands were also found. Significant differences between basal and PCP values in the VEH + PCP group were found at different time points. Remarkably, FPPQ pretreatment avoided all PCP-induced effects on oscillatory activity in freely moving rats except on the HFO band in dHPC (n = 5 for each area). Significant differences between the VEH + PCP and FPPQ + PCP groups were found in all cases but at different times (see statistical analyses in legend to Fig. 2). In parallel, PCP administration significantly increased locomotor activity in VEH pretreated rats (VEH + PCP; n = 6) as shown by the increased distance moved in the open field and the movement pattern representations (Fig. 2D). This effect was markedly attenuated in FPPQ pretreated rats (FPPQ + PCP; n = 5) (*P < .05; Mann Whitney test). Discussion The present work shows that the dual 5-HT3-R/5-HT6-R antagonist FPPQ reverses or prevents PCP-induced alterations of mPFC LFO and brain oscillatory activity. Both effects are predictive of potential clinical antipsychotic activity. Interestingly, antipsychotic effects appear to depend on the simultaneous blockade of 5-HT3-R and 5-HT6-R. As observed for firstand second-generation antipsychotics, including DA D2-R blockers and preferential 5-HT2A-R antagonists, FPPQ markedly reduced PCP-induced hyperlocomotion (Zajdel etal., 2021; present study). Likewise, FPPQ also reversed the decrease in LFO (0.15–4 Hz) induced by PCP in the mPFC of anesthetized rats. The decrease in LFO power induced by PCP is associated with the activation of thalamo-cortical circuits (Kargieman etal., 2007; Santana etal., 2011) after the preferential blockade of NMDA-R in GABAergic neurons of the reticular thalamic nucleus (Troyano-Rodríguez etal., 2014). Interestingly, firstand second-generation antipsychotic drugs, with varying affinities for D2-R and 5-HT2A-R, together with new agents also acting at 5-HT1A-R reverse the decrease in LFO induced by PCP, making this reversal a fingerprint of potential clinical antipsychotic action (Kargieman et al., 2007; Lladó-Pelfort et al., 2016; Van den Munkhof etal., 2017). Remarkably, the reversal by FPPQ appears to depend on the concurrent additive or synergistic blockade of 5-HT3-R and 5-HT6-R, since the respective antagonists OND and SB-399 885 failed to induce any significant change when administered alone but fully reversed PCP effects when administered together. The exact mechanism(s) responsible for this effect are unknown but may depend on the expression of 5-HT3-R and 5-HT6-R in brain elements involved in the control of oscillatory activity. Hence, in addition to the medullary nuclei involved in vomit control, such as the area postrema, 5-HT3-Rs are exclusively located in GABAergic interneurons of the neocortex and the hippocampal formation (Morales and Bloom, 1997; Puig etal., 2004). In the neocortex, 5-HT3-R–expressing GABA interneurons represent the largest population of GABA interneurons in upper layers (Puig etal., 2004; Santana and Artigas, 2017). The physiological stimulation of the serotonergic raphe nuclei evokes fast ionotropic excitatory responses in GABA cells expressing 5-HT3-R in the mPFC (Puig etal., 2004) and hippocampus (Varga etal., 2009), respectively. Despite their preferential localization in layers I– III, 5-HT3-R–expressing GABA interneurons tightly control the activity of layer V neurons projecting to subcortical structures, such as the midbrain monoamine nuclei (Santana and Artigas, 2017). Hence, the administration of the selective 5-HT3-R antagonist OND or the antidepressant drug vortioxetine (also blocking 5-HT3-R with high affinity) increased the discharge rate of approximately 70% of midbrain–projecting layer V pyramidal neurons in rat mPFC (Riga et al., 2016). A large population of pyramidal neurons in layer V of the same mPFC areas also project to dorsal and ventral striatum (caudate-putamen and NAc) (Gabbott etal., 2005), which show the highest density of 5-HT6-R in rat brain (Helboe etal., 2015). Therefore, although not tested experimentally, it is possible that GABA cells expressing 5-HT3-R Downloaded from https://academic.oup.com/ijnp/article/25/5/425/6504018 by Secretaria General Adjunta de Informatica user on 30 November 2022 428 | International Journal of Neuropsychopharmacology, 2022 also control the activity of layer V pyramidal neurons projecting to caudate-putamen and NAc. Hence, 5-HT3-R blockade would increase the cortical excitatory input onto medium-size spiny GABAergic striatal neurons expressing 5-HT6-R. The simultaneous blockade of both receptors would then affect nerve transmission in cognitive and affective basal ganglia circuits, which feed back to the neocortex via the thalamus, thus attenuating the increased thalamo-cortical activity induced byPCP. Additionally, 5-HT6-R are located in a much lower density in the neocortex and HPC, where they are expressed in pyramidal neurons and in 15% of GABAergic interneurons, most of which also express 5-HT3-R (Helboe etal., 2015). This regional and cellular localization represents an additional level of interaction between both receptors, which may add to the above-mentioned control of basal ganglia circuits. Interestingly, without altering oscillatory activity by itself, FPPQ was also able to prevent the increase in the power of various oscillatory bands evoked by PCP in the 3 forebrain areas examined (mPFC, NAc, and dHPC), where 5-HT3-R and 5-HT6-R are expressed. In the PFC, PCP moderately increased the power of theta and beta oscillations and markedly increased gamma and HFO powers. FPPQ pretreatment fully prevented PCP effect on theta, beta, and HFO and markedly attenuated the gamma elevation. In NAc, PCP significantly increased the power of theta, beta, and HFO, an effect fully prevented by FPPQ in all frequency bands. Likewise, in dHPC, PCP evoked a moderate yet nonsignificant decrease of the delta band and markedly increased the power of the gamma and high-frequency bands, both effects significantly attenuated by FPPQ pretreatment. These effects agree with previous data indicating an increase of gamma oscillations by NMDA-R antagonists, including PCP (Hakami etal. 2009; Jones et al., 2012), an effect partly antagonized by antipsychotic drugs, which by themselves reduced gamma power (Jones etal., 2012). Recently, Zajdel et al. (2021) found that FPPQ dosedependently prevented PCP-induced short-term memory deficits in the NORT. The effects of FPPQ were similar to those produced by the 5-HT6-R antagonist intepirdine. There is compelling evidence that the blockade of 5-HT3-R by OND also produces pro-cognitive effects in preclinical and clinical trials Figure 1. Representative recordings showing the reversal of phencyclidine (PCP)-induced disruption of low frequency oscillations (LFO; 0.15–4 Hz) in the medial prefrontal cortex (mPFC) by FPPQ and the combination ondansetron (OND) + SB399885, but not by OND or SB399885 alone (A–D). Black bars at the bottom of the panels show the time-periods when the power of LFO was calculated. (E) Effect of the i.v. administration of FPPQ (3mg/kg) on PCP-induced disruption of LFO in the mPFC (1-way ANOVA, reversal effect: F(4,16) = 14.33, P < .01; n = 5). (F–H) Effect of i.v. administration of OND (1.28mg/kg i.v.) (1-way ANOVA, PCP effect: F(4,12) = 153.01, P < .001; n = 4) (F), SB-399 885 (10mg/kg i.v.) (1-way ANOVA, PCP effect: F(4,12) = 29.34, P < .001; n = 4) (G), and their combination (H) (1-way ANOVA, reversal effect: F(4,16) = 8.17, P < .001; n = 5) on PCP-induced disruption of mPFC LFO. Note that only the combination of OND + SB399885 (but not either drug alone) reversed PCP effects. (I) Comparison between PCP + OND, PCP + SB-399 885, and PCP + OND + SB-399 885 groups. Two-way ANOVA (treatment [T] and time [t] as factors): T (F(2,10) = 7.15; P < .05), t (F(4,40) = 48.94; P < .001), Txt interaction (F(8,40) = 4.88; P < .001). (J) Comparison between PCP + FPPQ and PCP + OND + SB-399 885 groups. Two-way ANOVA (treatment [T] and time [t] as factors): T (F(1,8) = 1.18; ns), t (F(4,32) = 19.32; P < .001), Txt interaction (F(4,32) = 0.55; ns). Note that no differences between groups were found. *P < .05 vs basal values; #P < .05 vs PCP values (Duncan’s post-hoc tests). (K) Bar graph showing average effects of PCP on LFO in the mPFC as well as the reversal by the atypical antipsychotics clozapine (CLZ), olanzapine (OLZ), quetiapine (QTP), risperidone (RSP), ziprasidone (ZPS) and aripiprazole (ARI) (modified from Lladó-Pelfort etal., 2016). *P < .005 vs basal values; #P < .02 vs PCP. (L) Neutral-Red stained coronal section of the rat brain at the level of the site of recording in the mPFC (Paxinos and Watson, 2005). Downloaded from https://academic.oup.com/ijnp/article/25/5/425/6504018 by Secretaria General Adjunta de Informatica user on 30 November 2022 Copyedited by: Castañé et al. | 429 Figure 2. Oscillatory activity in (A) the medial prefrontal cortex (mPFC), (B) nucleus accumbens (NAc), and (C) dorsal hippocampus (dHPC) was measured after PCP administration (5mg/kg) in vehicle (VEH) and FPPQ (3mg/kg)-treated rats. Left arrow signals VEH/FPPQ administration, right arrow signals phencyclidine (PCP) administration. The figure includes Neutral-Red stained coronal sections of the rat brain at the level of the site of recording (Paxinos and Watson, 2005). Abbreviations: b, beta; d, delta; g, gamma; HFO, high-frequency oscillations; t, theta. Data are expressed as percentage of basal values (mean ± SEM). Two-way ANOVAs with treatment (T) and time (t) as factors were: (A) d: T (F(1,8) = 1.20; ns), t (F(19,152) = 1.68; P < .05), Txt (F(19,152) = 0.50; ns); t: T (F(1,9) = 9.94; P < .05), t (F(19,171) = 1.92; P < .05), Txt (F(19,171) = 1.39; ns); b: T (F(1,9) = = 3.09; ns), t (F(19,171) = 4.02; P < .001), Txt (F(19,171) = 1.72; P < .05); g: T (F(1,9) = 18.30; P < .01), t (F(19,171) = 49.06; P < .001), Txt (F(19,171) = 10.45; P < .001); HFO: T (F(1,9) = 17.41; P < .01), t (F(19,171) = 13.72; P< .001), Txt (F(19,171) = 6.58; P < .001). (B) d: T (F(1,8) = 0.51; ns), t (F(19,152) = 1.76; P < .05), Txt (F(19,152) = 1.08; ns); t: T (F(1,9) = 10.02; P < .05), t (F(19,171) = 7.18; P < .001), Txt (F(19,171) = 3.87; P < .001); b: T (F(1,9) = 6.77; P < .05), t (F(19,171) = 7.13; P < .001), Txt (F(19,171) = 4.24; P < .001); g: T (F(1,9) = 2.26; ns), t (F(19,171) = 2.33; P < .01), Txt (F(19,171) = 1.61; P = .057); HFO: T (F(1,9) = 13.41; P < .01), t (F(19,171) = 15.40; P < .001), Txt (F(19,171) = 9.53; P < .001). (C) d: T (F(1,8) = 0.00; ns), t (F(19,152) = 4.41; P < .001), Txt (F(19,152) = 0.62; ns); t: T (F(1,9) = .88; ns), t (F(19,171) = 2.74; P < .001), Txt (F(19,171) = 1.25; ns); b: T (F(1,9) = .00; ns), t (F(19,171) = 4.45; P < .001), Txt (F(19,171) = .65; ns); g: T (F(1,9) = 1.77; ns), t (F(19,171) = 25.71; P < .001), Txt (F(19,171) = 2.42; P < .01); HFO: T Downloaded from https://academic.oup.com/ijnp/article/25/5/425/6504018 by Secretaria General Adjunta de Informatica user on 30 November 2022 430 | International Journal of Neuropsychopharmacology, 2022 (Garay etal., 2016). In addition, the pro-cognitive effect of the antidepressant drug vortioxetine results from its blockade of 5-HT3-R in GABAergic interneurons of the mPFC (Sanchez etal., 2015). Therefore, synergistic or additive effects of the blockade of both 5-HT3-R and 5-HT6-R may also contribute to the procognitive effects ofFPPQ. In summary, and notwithstanding the limitation of using only male rats, the present observations indicate that FPPQ reverses the PCP-induced alterations in brain oscillatory activity though an entirely novel mechanism, that is, the simultaneous blockade of 5-HT3-R and 5-HT6-R. These observations, together with its pro-cognitive properties in the NORT (Zajdel etal., 2021), support the validity of FPPQ as a potential candidate for the treatment of schizophrenia. Acknowledgments We thank Verónica Paz for technical support. This work was supported by a grant agreement between IDIBAPS and Spherium, the CERCA Programme (Generalitat de Catalunya), Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), and Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya (2017SGR717). Interest Statement P.C.was PI of a grant agreement between IDIBAPS and Spherium. 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