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Bacterial meningitis in Finland, 1995-2014: a population-based observational study

Polkowska, Aleksandra,Toropainen, Maija,Ollgren, Jukka,Lyytikäinen, Outi,Nuorti, Pekka J

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1 Polkowska A, e al. BMJ Open 2017;0:e015080. doi:10.1136/bmjopen-2016-015080 Open Access Abs Ac Objec i es Bac e ial meningi is emains an impo an cause o mo bidi y and mo ali y wo ldwide. I s epidemiological cha ac e is ics, howe e , a e changing due o new accines and secula ends. Conjuga e accines agains Haemophilus in luenzae ype b and S ep ococcus pneumoniae (10- alen ) we e in oduced in 1986 and 2010 in Finland. We assessed he disease bu den and long- e m ends o i e common causes o bac e ial meningi is in a popula ion-based obse a ional s udy. Me hods A case was de ined as isola ion o S. pneumoniae, Neisse ia meningi idis, S ep ococcus agalac iae, Lis e ia monocy ogenes o H. in luenzae om ce eb ospinal luid and epo ed o na ional, popula ion- based labo a o y su eillance sys em du ing 1995–2014. We e alua ed changes in incidence a es (Poisson o nega i e binomial eg ession), case a ali y p opo ions (χ2) and age dis ibu ion o cases (Wilcoxon ank-sum). Resul s Du ing 1995–2014, S. pneumoniae and N. meningi idis accoun ed o 78% o he o al 1361 epo ed bac e ial meningi is cases. H. in luenzae accoun ed o 4% o cases (92% o isola es we e non- ype b). Du ing he s udy pe iod, he o e all a e o bac e ial meningi is pe 1 00 000 pe son-yea s dec eased om 1.88 cases in 1995 o 0.70 cases in 2014 (4% annual decline (95% CI 3% o 5%). This was p ima ily due o a 9% annual educ ion in a es o N. meningi idis (95% CI 7% o 10%) and 2% dec ease in S. pneumoniae (95% CI 1% o 4%). The median age o cases inc eased om 31 yea s in 1995–2004 o 43 yea s in 2005–2014 (p=0.0004). O e all case a ali y p opo ion (10%) did no change om 2004 o 2009 o 2010–2014. Conclusions Subs an ial dec eases in bac e ial meningi is we e associa ed wi h in an conjuga e accina ion agains pneumococcal meningi is and secula end in meningococcal meningi is in he absence o accina ion p og amme. Ongoing epidemiological su eillance is needed o iden i y ends, e alua e se o ype dis ibu ion, assess accine impac and de elop u u e accina ion s a egies. In oduc Ion Despi e he a ailabili y o accines, an i- bio ics and ad ances in in ensi e ca e, bac e ial meningi is emains an impo an cause o mo bidi y and mo ali y wo ldwide. Pe sis en neu ological sequelae including hea ing loss, neu opsychological impai - men o seizu es a e epo ed in 10%–30% o su i o s.1 The case a ali y p opo ion (CFP) anges om 5% o 30% o di e en bac e ia.2 3 Globally, S ep ococcus pneumoniae, Neis- se ia meningi idis and Haemophilus in luenzae a e he mos impo an causes o bac e ial meningi is, pa icula ly in young child en.4 5 Among neona es, he mos common cause o bac e ial meningi is is S. agalac iae,2 6 while Lis e ia monocy ogenes is impo an in newbo ns and elde ly pe sons wi h como - bidi ies.7 Howe e , he leading o ganisms causing bac e ial meningi is a y by age o he pa ien , ime and geog aphical loca ion.5 As he choice o empi ical an imic obial ea men o bac e ial meningi is should be based on local epidemiology, pa ien ’s age, p esence o isk ac o s and egional esis ance pa e ns,8–10 popula ion-based Bac e ial meningi is in Finland, 1995– 2014: a popula ion-based obse a ional s udy Aleksand a Polkowska,1 Maija To opainen,2 Jukka Ollg en,2 Ou i Lyy ikäinen,2 J. Pekka Nuo i1,2 o ci e: PolkowskaA, To opainenM, Ollg enJ, e al. Bac e ial meningi is in Finland, 1995–2014: a popula ion-based obse a ional s udy. BMJ Open 2017;0:e015080. doi:10.1136/ bmjopen-2016-015080 ►P epublica ion his o y and addi ional ma e ial a e a ailable. To iew, please isi he jou nal (h p:// dx. doi. o g/ 10. 1136/ bmjopen- 2016- 015080). Recei ed 9 No embe 2016 Re ised 27 Ma ch 2017 Accep ed 13 Ap il 2017 1School o Heal h Sciences, Uni e si y o Tampe e, Lääkä inka u, Tampe e, Finland 2Depa men o In ec ious Diseases, Na ional Ins i u e o Heal h and Wel a e (THL), Manne heimin ie, Helsinki, Finland Co espondence o P o . J. Pekka Nuo i; pekka. nuo i@ u a. i Resea ch S eng hs and limi a ions o his s udy ►This s udy desc ibes he epidemiological cha ac e is ics o  >1300 cases o bac e ial meningi is epo ed o na ional su eillance du ing 20 yea s in Finland. ►The s udy p o ides clinically impo an in o ma ion on he changing dis ibu ion o pa hogens and age o cases. ►The s udy documen s he sus ained popula ion impac o in an conjuga e accina ion agains Haemophilus in luenzae ype b; and in oduc ion o 10- alen pneumococcal conjuga e accina ion on educing he bu den o bac e ial meningi is, as well as decline in meningococcal meningi is due o secula end. As he da a we e om labo a o y- based su eillance sys em, clinical in o ma ion such as se e i y o ea men was no a ailable. ►Incidence a e o bac e ial meningi is may be unde es ima ed since cases diagnosed by PCR o an igen de ec ion and cul u e-nega i e meningi is cases diagnosed based on clinical symp oms and indings we e no included in he da ase . g oup.bmj.com on Augus 7, 2017 - Published by h p://bmjopen.bmj.com/Downloaded om 2Polkowska A, e al. BMJ Open 2017;0:e015080. doi:10.1136/bmjopen-2016-015080 Open Access su eillance da a a e impo an o help in o mula ing clinical guidelines. The in oduc ion o e ec i e p o ein conjuga e accines agains H. in luenzae ype b (Hib), S. pneumoniae and N. meningi idis has changed he epidemiology o bac e ial meningi is in many coun ies.11 12 In Finland, uni e sal accina ion agains Hib since 1986 esul ed in apid elim- ina ion o he disease13 and in oduc ion o he 10- alen pneumococcal conjuga e accine (PCV10) in Sep embe 2010 has esul ed in subs an ial educ ion in accine- ype in asi e disease.14 15 Meningococcal conjuga e accines (MCVs) ha e no been in oduced in o Finnish Na ional Vaccina ion P og amme (NVP). Howe e , meningo- coccal polysaccha ide accine has been o e ed o mili a y consc ip s since 1982. To p o ide in o ma ion o de eloping u u e p e en- ion s a egies and o help in o mula ing clinical guidelines, we conduc ed a popula ion-based obse a- ional s udy o de e mine he con ibu ion o speci ic pa hogens o he o al bac e ial meningi is disease bu den and o assess long- e m ends in he incidence o common ae iologies in Finland du ing 1995–2014. Ma e Ials and Me hods da a sou ces Since 1995, all clinical mic obiology labo a o ies in Finland ha e had legal obliga ion o epo mic obial isola ions om blood and/o ce eb ospinal luid (CSF) o he Na ional In ec ious Diseases Regis e (NIDR)—a popula ion-based, elec onic labo a o y su eillance sys em main ained by he Na ional Ins i u e o Heal h and Wel a e (THL). Rou inely collec ed in o ma ion include he mic obe, specimen da e, da e o bi h, sex, place o esidence and unique Pe sonal Iden i y Code (PIC). Fo blood o CSF indings conce ning S. pneumo- niae, S. agalac iae, N. meningi idis, L. monocy ogenes o H. in luenzae, mul iple no i ica ions wi h he same PIC and mic obe a e me ged in o one case i hey occu ed wi hin 3 mon hs o he i s no i ica ion. Since 2004, in o ma ion on i al s a us a e episode is ou inely ob ained om he Popula ion In o ma ion Sys em. All clinical mic obiology labo a o ies also submi isola es om epo ed cases o THL e e ence labo a o ies o species e i ica ion and cha ac e isa ion o he isola es including se o yping o se og ouping. Since 2004, se o yping esul s a e linked o NIDR no i ica ions by using he PIC. An imic obial suscep ibili y da a we e no a ailable. case de ini ions We de ined a case o bac e ial meningi is as isola ion o S. pneumoniae, S. agalac iae, N. meningi idis, L. monocy ogenes o H. in luenzae om CSF and no i ied o NIDR om 1995 h ough 2014. Fo cases epo ed du ing 2004–2014, we calcula ed he pa hogen-speci ic 30-day CFP as numbe o cases esul ing in dea h wi hin 30 days om he i s posi i e CSF cul u e, di ided by all cases. We calcula ed he p opo ions o S. pneumoniae, N. meningi idis and H. in luenzae cases due o accine-p e en - able se o ypes/se og oups du ing 2004–2014. Se o ypes co e ed in PCV10 a e he ollowing: 1, 4, 5, 6B, 7F, 9V, 14, 18C, 19F and 23F; he 13- alen PCV13 adds se o ypes 3, 6A and 19A. Vaccine-p e en able meningococcal se o- g oups include hose in he quad i alen MCV (MCV-4, A, C, W and Y) and se og oup B isola es a ge ed by no el p o ein-based accines (MenB). Fo H. in luenzae, ype b was conside ed accine p e en able. s a is ical analysis By using da a om he Popula ion In o ma ion Sys em as denomina o s, we calcula ed pa hogen-speci ic and age-speci ic annual incidence a es. Poisson eg ession was used o es o log-linea end in a es o bac e- ial meningi is du ing 1995–2014. Incidence a e a ios (IRRs), hei 95% CI and p alues o yea ly changes we e calcula ed using ime (yea ) as a con inuous explana o y a iable in he Poisson model. When app op ia e, we used nega i e binomial eg ession o co ec o o e dispe - sion o da a. To compa e age dis ibu ion o cases ac oss yea s, we used Wilcoxon ank-sum es . To assess changes in CFP, we used χ2 analyses; p alue <0.05 was conside ed s a is ically signi ican . All analyses we e done wi h STATA e sion 13 and Mic oso Excel 2013. e hical conside a ions Da a used in he analysis we e collec ed as a pa o na ional ou ine su eillance which alls unde he exis ing manda e o THL. No o mal Ins i u ional Re iew Boa d e iew was equi ed o his s udy. Pe sonal iden i- ie s we e emo ed a e linkage wi h i al s a us da a. esul s o e all incidence a es o bac e ial meningi is F om 1995 o 2014, 1361 cases o bac e ial meningi is caused by S. pneumoniae, N. meningi idis, S. agalac iae, L. monocy ogenes o H. in luenzae we e epo ed (mean inci- dence a e, 1.29 cases/100 000 pe son-yea s, able 1). S. pneumoniae and N. meningi idis we e he mos common ae iologies accoun ing o 78% (1061/1361) o cases. The median age o cases inc eased om 31 yea s in 1995–2004 o 43 yea s in 2005–2014 (p=0.0004). Ra es we e highe in men han women (1.52 s 1.07 cases/100 000 pe son- yea s; IRR 1.4, 95% CI 1.3 o 1.6)). The mean annual a es o all bac e ial meningi is anged om 1.97 in 1996 o 0.70 cases/100 000 pe son- yea s in 2014, wi h an annual dec ease o 4% (95% CI −3% o −5%, able 1). Du ing 2004–2014, 65 pa ien s died wi hin 30 days om cul u e (CFP, 10% (65/633)). The e was no change in 30-day CFP om 2004–2009 (11% (43/402) o 2010–2014 (10% (22/231), p=0.22. cha ac e is ic o bac e ial meningi is by age g oup Child en <2 yea s o age accoun ed o 20% o cases (268/1361) and had he highes incidence a e (11.38 cases/100 000 pe son-yea s, able 1). The mos common g oup.bmj.com on Augus 7, 2017 - Published by h p://bmjopen.bmj.com/Downloaded om 3 Polkowska A, e al. BMJ Open 2017;0:e015080. doi:10.1136/bmjopen-2016-015080 Open Access Table 1 Numbe o cases (n), incidence a es pe 100 000 pe son-yea s (IR) and mean annual ela i e change in incidence o bac e ial meningi is, acco ding o age g oup (yea s), Finland, 1995–2014 1995–1999 2000–2004 2005–2009 2010–2014 1995–2014 1995–2014 n IR n IR n IR n IR n IR % change* 95% CI S ep ococcus pneumoniae <2 26 4.32 25 4.43 18 3.05 14 2.33 83 3.52 −4 −7 o 0 2–4 6 0.63 3 0.35 6 0.69 3 0.33 18 0.50 −1 −8 o7 5–17 13 0.31 11 0.26 7 0.17 2 0.05 33 0.20 −7 −13 o1 18–49 59 0.50 54 0.48 35 0.32 30 0.27 178 0.40 −4 −6 o 1 50–64 41 0.91 52 1.00 56 0.99 37 0.65 186 0.88 −2 −4 o 1 ≥65 25 0.67 23 0.57 39 0.89 26 0.51 113 0.66 −1 −4 o 2 All age g oups 170 0.55 168 0.65 161 0.61 112 0.41 611 0.58 −2 −4 o 1 Neisse ia meningi idis <2 23 3.83 23 4.07 8 1.36 14 2.33 68 2.89 −4 −8 o 0 2–4 19 1.98 11 1.27 12 1.38 6 0.66 48 1.33 −6 −10 o −1 5–17 37 0.88 16 0.38 24 0.60 7 0.18 84 0.52 −8 −14 o −3 18–49 93 0.79 46 0.41 42 0.38 14 0.13 195 0.43 −10 −13 o −8 50–64 15 0.33 15 0.29 7 0.12 2 0.04 39 0.18 −12 −17 o −6 ≥65 6 0.16 3 0.07 4 0.09 3 0.06 16 0.09 −7 −14 o 2 All age g oups 193 0.62 114 0.44 97 0.37 46 0.17 450 0.43 −9 −10 o −7 Haemophilus in luenzae <2 4 0.67 3 0.53 2 0.34 1 0.17 10 0.42 −7 −17 o 4 2–4 0 0.00 3 0.35 0 0.00 0 0.00 3 0.08 NA NA 5–17 5 0.12 4 0.10 0 0.00 2 0.05 11 0.07 −8 −17 o 3 18–49 4 0.03 2 0.02 2 0.02 6 0.05 14 0.03 5 −5 o 15 50–64 3 0.07 3 0.06 2 0.04 2 0.04 10 0.05 −3 −13 o 8 ≥65 2 0.05 0 0.00 7 0.16 1 0.02 10 0.06 1 −9 o 12 All age g oups 18 0.06 15 0.06 13 0.05 12 0.04 58 0.06 −2 −7 o 2 S. agalac iae <2 25 4.16 24 4.25 32 5.43 25 4.16 106 4.50 0 −3 o 5 2–4 1 0.10 0 0.00 0 0.00 0 0.00 1 0.03 NA NA 5–17 0 0.00 1 0.02 0 0.00 0 0.00 1 0.01 NA NA Con inued g oup.bmj.com on Augus 7, 2017 - Published by h p://bmjopen.bmj.com/Downloaded om 4Polkowska A, e al. BMJ Open 2017;0:e015080. doi:10.1136/bmjopen-2016-015080 Open Access 1995–1999 2000–2004 2005–2009 2010–2014 1995–2014 1995–2014 n IR n IR n IR n IR n IR % change* 95% CI 18–49 2 0.02 1 0.01 1 0.01 2 0.02 6 0.01 1 −12 o 16 50–64 4 0.09 2 0.04 7 0.12 3 0.05 16 0.08 1 −9 o 8 ≥65 0 0.00 7 0.17 1 0.02 3 0.06 11 0.06 −2 −11 o 9 All age g oups 32 0.10 35 0.13 41 0.15 33 0.12 141 0.13 0 −3 o 3 Lis e ia monocy ogenes <2 1 0.17 0 0.00 0 0.00 0 0.00 1 0.04 NA NA 2–4 0 0.00 0 0.00 0 0.00 0 0.00 0 0.00 NA NA 5–17 1 0.02 0 0.00 0 0.00 0 0.00 1 0.01 NA NA 18–49 9 0.08 3 0.03 0 0.00 3 0.03 15 0.03 −11 −19 o −3 50–64 10 0.22 3 0.06 6 0.11 4 0.07 23 0.11 −6 −13 o 1 ≥65 14 0.37 13 0.32 13 0.30 21 0.42 61 0.35 0 −4 o 4 All age g oups 35 0.11 19 0.07 19 0.07 28 0.10 101 0.10 −2 −5 o 1 To al bac e ial meningi is <2 79 13.14 75 13.28 60 10.18 54 8.99 268 11.38 −2 −4 o 1 2–4 26 2.71 17 1.97 18 2.07 9 0.98 70 1.94 −5 −10 o 0 5–17 56 1.33 32 0.77 31 0.77 11 0.28 130 0.80 −8 −12 o −4 18–49 167 1.43 106 0.94 80 0.73 55 0.50 408 0.91 −7 −8 o −5 50–64 73 1.63 75 1.44 78 1.37 48 0.84 274 1.30 −4 −6 o −2 ≥65 47 1.25 46 1.15 64 1.46 54 1.07 211 1.23 −1 −4 o 1 All age g oups 448 1.45 351 1.35 331 1.25 231 0.85 1361 1.29 −4 −3 o −5 *Mean annual ela i e change in incidence calcula ed by Poisson eg ession o nega i e binomial eg ession. NA,no applicable. Table 1 Con inued g oup.bmj.com on Augus 7, 2017 - Published by h p://bmjopen.bmj.com/Downloaded om 5 Polkowska A, e al. BMJ Open 2017;0:e015080. doi:10.1136/bmjopen-2016-015080 Open Access pa hogens in his age g oup we e S. agalac iae (4.50 cases/100 000 pe son-yea s) and S. pneumoniae (3.52 cases/100 000 pe son-yea s, igu e 1). F om 1995 o 2014, he a e o bac e ial meningi is in his age g oup dec eased by 2% annually (95% CI −4% o −1%, able 1). The a e age 30-day CFP in 2004–2014 was 2% (3/140). In child en 2–4 yea s o age, 70 cases (5%) o bac e ial meningi is we e epo ed du ing 1995 o 2014 (1.94 cases/100 000 pe son- yea s). The mos common pa hogens in his age g oup we e N. meningi idis (1.33 cases/100 000 pe son-yea s) and S. pneumoniae (0.50 cases/100 000 pe son-yea s, able 1). Du ing he s udy pe iod, he a e o all meningi is did no change signi ican ly ( able 1). The 30-day CFP in 2004–2014 was 14% (4/128); all ou dea hs we e due o N. meningi idis. Child en 5–17 yea s o age accoun ed o 130 cases (9%) o bac e ial meningi is and had he lowes a e (0.80 cases/100 000 pe son-yea s, able 1). N. meningi idis and S. pneumoniae we e he main causes (0.52 and 0.20 cases/100 000 pe son-yea s, espec i ely, igu e 1). F om 1995 o 2014, he a e o bac e ial meningi is dec eased by 8% annually (95% CI −12% o −4%, able 1). The 30-day CFP was 7% (3/45); all h ee a al cases we e due o N. meningi idis. Adul s 18–49 yea s o age accoun ed o 408 cases (30%) o bac e ial meningi is (0.91 cases/100 000 pe son- yea s, able 1). N. meningi idis and S. pneumoniae caused mos o he cases ( igu e 1), wi h incidence a es 0.43 and 0.40 cases/100 000 pe son-yea s, espec i ely. Du ing 1995–2014, he o e all a e dec eased by 7% annually (95% CI −8% o −5%, able 1). The 30-day CFP was 8% (13/152), wi h nine dea hs due o S. pneumoniae in ec ion. Among pe sons 50–64 yea s o age, he e we e 274 cases (20%) o bac e ial meningi is (1.30 cases/100 000 pe son- yea s, able 1), o which 186 cases (68%) we e caused by S. pneumoniae (0.88 cases/100 000 pe son-yea s, igu e 1). Du ing he s udy pe iod, he o e all a e dec eased by 4% annually (95% CI −6% o −2%, able 1). The 30-day CFP was 13% (18/143), wi h mos a al cases a ibu able o S. pneumoniae (16 dea hs). In adul s ≥65 yea s o age, he e we e 211 cases (15%) o bac e ial meningi is (1.23 cases/100 000 pe son- yea s, able 1). S. pneumoniae caused 53% (113/211) o he cases (0.66 cases/100 000 pe son-yea s), ollowed by L. monocy ogenes. The e was no signi ican change in he o e all a e du ing 1995–2014 ( able 1). This age g oup had he highes 30-day CFP (19%, 24/125). Hal o he a al cases we e due o S. pneumoniae (12 dea hs); L. mono- cy ogenes caused 10 dea hs. causes o bac e ial meningi is S ep ococcus pneumoniae F om 1995 o 2014, 611 cases o pneumococcal menin- gi is we e epo ed. Median age was 48 yea s; 57% o cases we e male (male o emale IRR, 1.4 95% CI 1.2 o 1.6, able 2). The o e all annual a e pe 1 00 000 pe son-yea s dec eased om 0.70 in 1995 o 0.26 in 2014 ( igu e 2), a 2% annual dec ease (95% CI −4% o −1%, able 1). The incidence o pneumococcal meningi is dec eased annually by 4% (95% CI −7% o 0%), 7% (95% CI −13% o −1%) and 4% (95% CI −6% o −1%) in age g oups <2 yea s, 5–17 yea s and 18–49 yea s, espec i ely. Du ing 2004–2014, S. pneumoniae accoun ed o 58% (38/65) o a al cases (30-day CFP 12%, 38/308). O he 308 pneumococcal meningi is cases epo ed du ing 2004–2014, in o ma ion on se o ype was a ailable o 296 (96%). The p opo ion o cases caused by PCV10 se o ypes dec eased om 61% (35/57) in 2004–2005 o 15% (9/36) in 2013–2014. PCV13 se o ypes accoun ed o 70% (40/57) cases in 2004–2005 and 44% (16/36) in 2013–2014. In child en less han 2 yea s, p opo ion o meningi is cases caused by PCV10 se o ypes dec eased om 75% (9/12) in 2004–2005 o 20% (1/5) in 2013– 2014. In 2014, no meningi is cases we e caused by PCV10 se o ypes. Neisse ia meningi idis Du ing he s udy pe iod, meningococcal meningi is accoun ed o 450 cases (0.43 cases/100 000 pe son-yea s) ( able 1). Median age was 18 yea s and 60% o cases we e male (male o emale IRR 1.5, 95% CI 1.3 o 1.9, able 2). The o e all annual incidence pe 1 00 000 pe son- yea s dec eased om 0.88 in 1995 o 0.07 in 2014; he annual dec ease was −9% (95% CI −7% o −10%, able 1). The decline occu ed in all age-g oups excep in <2 yea s Figu e 1 P opo ions o bac e ial meningi is cases caused by i e pa hogens acco ding o age g oup, Finland, 1995–2014. g oup.bmj.com on Augus 7, 2017 - Published by h p://bmjopen.bmj.com/Downloaded om 6Polkowska A, e al. BMJ Open 2017;0:e015080. doi:10.1136/bmjopen-2016-015080 Open Access and ≥65 yea s o age. The incidence dec eased annually by 6% (95% CI −1% o −10%), 8% (95% CI −3% o −14%), 10% (95% CI −8% o −13%) and 12% (95% CI −8% o −13%) in age g oups 2–4 yea s, 5–17 yea s, 18–49 yea s and 50–64 yea s, espec i ely. The o e all 30-day CFP was 9% (14/163) and anged om 3% (1/29) among chil- d en aged 0–1 yea s o 21% (4/19) among child en aged 2–4 yea s. Du ing 2004–2014, in o ma ion on N. meningi idis se og oups was a ailable o 99% o cases (161/163). Se og oup B accoun ed o 85% (137/161) o isola es, C 11% (17/161) and Y 4% (7/161). In child en <2 yea s, se og oup B caused 96% (26/27) o cases. MCV-4 and MenB accine se og oups caused 15% (24/161) and 85% (137/161) o all cases, espec i ely. Haemophilus in luenzae F om 1995 o 2014, 58 cases o H. in luenzae we e epo ed (0.06 cases/100 000 pe son-yea s, able 1). Median age was 29 yea s and male o emale IRR was 1.0 (95% CI 0.6 o 1.7, able 2). The incidence a e anged om 0.0 cases pe 1 00 000 pe son-yea s in 2010 o 0.25 cases in 2007 ( igu e 2). Ra es in all age g oups we e s able. F om 2004 o 2014, he e we e no dea hs due o H. in luenzae. In 2004–2014, non-encapsula ed H. in luenzae accoun ed o 69% (18/26) , se o ype 23% (6/26) and ype b 8% (2/26) o isola es. S ep ococcus agalac iae In ec ion wi h S. agalac iae accoun ed o 141 cases o meningi is (0.13 cases/100 000 pe son-yea s), including 24 ea ly-onse cases and 78 la e-onse cases ( able 1). The median age o cases was 30 days; male o emale IRR was 1.03 (95% CI 0.7 o 1.4) ( able 2). Du ing he s udy Table 2 Cha ac e is ics o bac e ial meningi is cases, Finland, 1995–2014 Cha ac e is ics S. pneumoniae N. meningi idis S. agalac iae L. monocy ogenes H. in luenzae To al Gende , no o cases (% o o al) Male 347 (57) 268 (60) 70 (50) 71 (70) 28 (48) 784 (58) Female 264 (43) 182 (40) 71 (50) 30 (30) 30 (52) 577 (42) Age (yea s) Median 48 18 0 68 29 36 IQR 28–62 4–35 0 56–74 6–54 5–58 Case a ali y* No o dea hs (no o cases) 38 (308) 14 (163) 2 (86) 11 (50) 0 (26) 65 (633) Case a ali y p opo ion (%) 12.3 8.6 2.3 22 0 10.3 *Da a a e o cases epo ed du ing 2004–2014. Figu e 2 Incidence a e (pe 100 000 pe son-yea s) o bac e ial meningi is by yea and pa hogen, Finland, 1995–2014. g oup.bmj.com on Augus 7, 2017 - Published by h p://bmjopen.bmj.com/Downloaded om 7 Polkowska A, e al. BMJ Open 2017;0:e015080. doi:10.1136/bmjopen-2016-015080 Open Access pe iod, annual a es anged om 0.06 cases/100 000 pe son-yea s in 1995 o 0.17 cases in 2014 ( igu e 2), bu o e all a es o S. agalac iae did no change signi ican ly (p=0.97, able 1). Du ing 2004–2014, he 30-day CFP was 2% (2/86). Lis e ia monocy ogenes Du ing he s udy pe iod, L. monocy ogenes caused 101 cases o meningi is (0.13 cases/100 000 pe son-yea s), mos ly among elde ly pe sons (median age, 68 yea s). O cases, 70% we e men (male o emale IRR 2.5, 95% CI 1.6 o 3.9, able 2). O e all incidence a es o Lis e ia meningi is did no a y signi ican ly du ing he s udy pe iod, anging om 0.04 o 0.21/100 000 pe son-yea s ( able 1). The o e all 30-day CFP was 22% (11/50) and 28% (10/36) in pe sons ≥65 yea s o age. dIscussIon Du ing 1995–2014, he mos common causes o bac e ial meningi is in Finland we e S. pneumoniae and N. meningi - idis. Howe e , con ibu ion o speci ic pa hogens o he disease bu den a ied subs an ially by age. As in o he de eloped coun ies, S. agalac iae was he mos common cause o bac e ial meningi is in child en <1 yea s o age.6 The mean age o cases inc eased signi ican ly du ing he s udy pe iod mainly because o he dec ease in inci- dence in child en associa ed wi h PCV10 p og amme and declining secula end in meningococcal meningi is. Du ing he s udy pe iod, signi ican declines we e seen in o e all incidence o bac e ial meningi is—p ima ily due o dec eases in a es o N. meningi is and S. pneu- moniae. O in e es , he dec ease in incidence o N. meningi idis was g ea e han o pneumococcal menin- gi is, al hough he e is no ou ine accina ion p og amme o meningococcal disease in Finland. Changes in a es o meningococcal disease ha e also been obse ed in o he coun ies in Eu ope and wo ldwide.16 17 The easons o hese declines in incidence a e no clea bu may be ela ed o popula ion immuni y o ci cula ing s ains, changes in colonising o ganisms in he nasopha ynx o inc easing use o in luenza accine. Also, changes in beha iou al isk ac o s such as lowe p e alence o smoking o c owding migh con ibu e.18 19 In some coun ies, dec eases we e ela ed o meningococcal acci- na ion. A e he in oduc ion o conjuga e se og oup C meningococcal accine, accine se og oup disease nea ly disappea ed in England20 and he Ne he lands.21 Di ec and indi ec (he d p o ec ion) accine e ec s we e also epo ed om o he Eu opean coun ies including Spain, I eland and Belgium.22 23 Immunisa ion o high isk g oups wi h ecen ly licensed p o ein-based accines a ge ed agains meningococcal se og oup B migh also be conside ed in Finland. Howe e , upda ed cos -e ec i e analysis is needed o decision-making abou in oduc- ion o meningococcal accina ion p og ams. Be o e he in oduc ion o PCV10, conside able a i- a ion in pneumococcal meningi is incidence a es was seen. As he e we e no majo changes in su eillance o diagnos ic p ac ices in Finland, hese changes may be ela ed o eme gence o new se o ypes, selec i e p essu e om an ibio ic use o na u al luc ua ion in se o ypes.24–26 The decline in pneumococcal meningi is incidence in child en <2 yea s o age was associa ed wi h in oduc ion o PCV10 in he NVP in 201015; PCV10 se o- ypes in his age g oup we e signi ican ly educed and by 2014 no accine se o ype meningi is cases we e epo ed. In accine-eligible child en, he o e all a e o pneumo- coccal meningi is was educed by 46% as a esul o a 69% educ ion in PCV10- ype meningi is.15 Many s udies in USA and Eu ope ha e also documen ed signi ican declines in he incidence o pneumococcal meningi is in bo h accina ed and un accina ed g oups a e in oduc- ion o PCV p og ammes.11 12 27–29 In Finland, i migh be possible o achie e u he educ ions wi h highe alency conjuga e accine o mula ions. The incidence a e o L. monocy ogenes, N. meningi idis and S. pneumoniae was highe in men han women. L. monocy ogenes meningi is cases we e 2.5 imes mo e likely o be men. Highe a es o lis e iosis in males ha e also been obse ed in o he s udies.7 Howe e , he easons a e unknown, bu may be ela ed o highe p e alence o unde lying condi ions, alcoholism among men and li e diseases (including alcoholic ci hosis).30 In pneumo- coccal and meningococcal meningi is, possible easons may be highe p e alence o unde lying medical condi- ions,smoking and alcoholism.31 As lis e iosis is p ima ily ansmi ed h ough con amina ed ood, impo an p e en ion e o s include heal h educa ion abou die a y guidelines o high isk g oups, such as p egnan women and he elde ly.32 The o e all 30-day CFP o meningi is did no change signi ican ly du ing 1995–2014. Howe e , he unchanged CFP may be ela ed o he al e ed age dis ibu ion o cases. Olde age is associa ed wi h highe isk o poo ou come.33 In addi ion, pa hogen dis ibu ion has changed and he case a ali y o meningococcal menin- gi is is lowe compa ed wi h pneumococcal meningi is. The small numbe o a al cases in ou s udy did no allow assessing changes in CFP by age g oup and pa hogen. The 30-day CFP was highes o L. monocy ogenes (22%), which is compa able wi h esul s om he Ne he lands and Spain.7 34 Mos o he a al cases o bac e ial meningi is in pe sons ≥50 yea s we e a ibu able o S. pneumoniae. Cases who had pneumococcal meningi is we e olde han hose who we e in ec ed wi h o he encapsula ed bac e ia and likely had highe p e alence o como bidi ies inc easing he isk o pneumococcal in ec ion and poo ou come.35 Because o lack o clinical da a, we could no assess he po en ial impac o ea men changes, such as dexa- me hasone use, on case a ali y. The ela i ely high CFP emphasises he impo ance o immedia e ini ia ion o ea men and suppo i e ca e a e diagnosis o imp o e ou come o bac e ial meningi is. As expec ed, H. in luenzae was he leas common cause o bac e ial meningi is. Howe e , he s able numbe o cases g oup.bmj.com on Augus 7, 2017 - Published by h p://bmjopen.bmj.com/Downloaded om 8Polkowska A, e al. BMJ Open 2017;0:e015080. doi:10.1136/bmjopen-2016-015080 Open Access o e 20 yea s sugges exis ence o small g oup o indi id- uals wi h isk ac o s o H. in luenzae (such as ch onic espi a o y disease and impai ed immuni y).36 Conju- ga e accina ion has nea ly elimina ed Hib meningi is in many high-income coun ies.37 38 Howe e , changes in he epidemiology o in asi e H. in luenzae ha e been obse ed and cu en ly mos cases occu in adul s39 and non-encap- sula ed, non- ypable H. in luenzae ha e domina ed since 2004. Because he da a on labo a o y con i med cases a e ansmi ed elec onically di ec ly om he clinical mic o- biology labo a o ies’ da abase o he na ional su eillance da abase, a s eng h o ou s udy is comp ehensi e case asce ainmen . In addi ion, almos all isola es o N. meningi idis, H. in luenzae and S. pneumoniae (98%) we e a ailable o se o yping/g ouping a THL e e ence labo a o y. Howe e , ou s udy has se e al limi a ions. As he da a we e om labo a o y-based su eillance sys em, in o ma ion on clinical p esen a ion o ea men was no a ailable. The e o e, cul u e-nega i e meningi is cases diagnosed on he basis o clinical symp oms and indings we e no included in he analysis da ase . In addi ion, cases diagnosed by PCR o an igen de ec ion we e no included. As CSF cul u es a e nega i e in 11%–30% o pa ien s wi h bac e ial meningi is,40 he o al numbe o meningi is cases is unde es ima ed. Ano he limi a ion is ha NIDR da abase does no include in o ma ion on he cause o dea h. Howe e , mos o dea hs associa ed wi h bac e ial meningi is occu ea ly (wi hin 14 days o admis- sion), sugges ing ha hey we e ela ed o he in ec ion.41 In conclusion, his s udy desc ibes he epidemiolog- ical cha ac e is ics o >1300 cases o bac e ial meningi is epo ed o na ional su eillance o e 20 yea s. I docu- men s he sus ained popula ion impac o in an conjuga e accina ion agains Hib and in oduc ion o PCV on educing bu den o bac e ial meningi is as well as decline in meningococcal meningi is due o secula end. Howe e , disease bu den had shi ed o olde people and no changes in he o e all p opo ion o a al cases we e seen. Da a on changes in causa i e o ganisms and age dis ibu ion o meningi is cases a e impo an o e al- ua ing clinical guidelines o empi ical an ibio ic he apy in bac e ial meningi is. Con inued epidemiological su eillance is necessa y o moni o changing ends and se o ype dis ibu ion, assessing he impac o accina ion p og ams and de eloping u u e accina ion s a egies. Con ibu o s S udy concep and design: AP, OL, PN. Acquisi ion o da a: MT, JO, OL, PN. Analysis and in e p e a ion o da a: AP, MT, JO, OL, PN. D a ing o he manusc ip : AP, PN. C i ical e ision o he manusc ip o impo an in ellec ual con en : AP, MT, JO, OL, PN. S a is ical analysis: AP, JO. Ob ained unding: PN. S udy supe ision: PN. Final app o al: AP, MT, JO, OL, PN. Funding This s udy was suppo ed by he School o Heal h Sciences, Uni e si y o Tampe e and he Na ional Ins i u e o Heal h and Wel a e (THL) in Helsinki, Finland. Compe ing in e es s None decla ed. E hics app o al Da a used in he analysis we e collec ed as a pa o su eillance and in ec ion con ol ac i i ies which alls unde he exis ing manda e o he Na ional Ins i u e o Heal h and Wel a e (THL). P o enance and pee e iew No commissioned; ex e nally pee e iewed. Da a sha ing s a emen No addi ional da a a e a ailable. Open Access This is an Open Access a icle dis ibu ed in acco dance wi h he C ea i e Commons A ibu ion Non Comme cial (CC BY-NC 4.0) license, which pe mi s o he s o dis ibu e, emix, adap , build upon his wo k non-comme cially, and license hei de i a i e wo ks on di e en e ms, p o ided he o iginal wo k is p ope ly ci ed and he use is non-comme cial. See: h p:// c ea i ecommons. o g/ licenses/ by- nc/ 4.0/ © A icle au ho (s) (o hei employe (s) unless o he wise s a ed in he ex o he a icle) 2017. All igh s ese ed. No comme cial use is pe mi ed unless o he wise exp essly g an ed. e e ences 1. Edmond K, Cla k A, Ko czak VS, e al. Global and egional isk o disabling sequelae om bac e ial meningi is: a sys ema ic e iew and me a-analysis. Lance In ec Dis 2010;10:317–28. 2. Sáez-Llo ens X, McC acken GH. Bac e ial meningi is in child en. Lance 2003;361:2139–48. 3. de Jonge RC, an Fu h AM, Wassenaa M, e al. 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