INAUGURAL-DISSERTATION
zur
Erlangung der Doktorwürde
der
Naturwissenschaftlich-Mathematischen Gesamtfakultät
der
Ruprecht-Karls-Universität
Heidelberg
vorgelegt von
Hui Shen
aus
Wuhan, Volksrepublik China
Tag der mündlichen Prüfung:
Regulation der Transkription des hspA Gens
in Stigmatella aurantiaca
und
Analyse der Funktion des HspA Proteins
Gutachter: Prof. Dr. Hans Ulrich Schairer
Prof. Dr. Richard Herrmann
Dissertation
submitted to the
Combined Faculties for the Natural Sciences and for Mathematics
of the Rupertus Carola University of
Heidelberg, Germany
for the degree of
Doctor of Natural Sciences
presented by
Hui Shen
born in Wuhan, People’s republic of China
Heidelberg, 1999
Transcriptional regulation of hspA gene
in Stigmatella aurantiaca
and
Function analysis of HspA protein
Examiners: Prof. Dr. Hans Ulrich Schairer
Prof. Dr. Richard Herrmann
Acknowledgments
Sincere thanks to my supervisor Prof. Dr. Hans Ulrich Schairer for setting up the project and offering
me the working place. I greatly appreciate his scientific supervision and practical advice throughout
my work and his important comments on the manuscript.
I would like to thank the team of Prof. Dr. Johannes Buchner in Regensburg University, Germany, to
offer me the opportunity to perform the chaperone assays with HspA protein in their lab. I specially
thank Monika Ehrnsperger, Holger Graller and Christian Myr who greatly helped me during the
work.
Many thanks to Dr. Heinrich Lünsdorf in GBF institute, Germany, who carefully examined the
ultrastructure of S. aurantiaca wild type and hspA deletion mutant cells and spores.
My special thanks go to the team of Prof. Dr. Richard Herrmann for the sequence proof of some PCR
products, and also go to the team of Dr. Rainer Frank for the sequence proof, oligo synthesis, and for
the mass spectrometry measurement.
Thanks are given to the whole team of Prof. Dr. Hans Ulrich Schairer, my colleagues, especially to
Wulf Plaga and Irmela Stamm for sharing the electroporation protocol for S. aurantiaca before it was
published, to Bianca Maxl for providing plasmid #9, to Barbara Silakowski for providing the sigma B
mutant strain BS53, to Susanne Müller and Andreas Leclerque for proofreading this manuscript, for
many fruitful discussion, and for organizing the lab.
Also I would like to thank Giacomo Cavalli for introducing me the techniques in RNA manipulation,
thank Inhua Chen to correct my English manuscript, thank the all people of ZMBH who helped me
during my thesis work.
A thousand thanks to Jianbing Zhang, my husband, for his help throughout my whole life not only the
whole time of this work, to my parents and my sisters for their support of my work and taking care of
my daughter, and to my daughter Yaxin for excusing her mother who often has not enough time for
her.
